(Linear) (Six-base) MAPSORT of: KIEE5204.seq Check: 469 from: 1 to: 624 KIEE5204.orf With 182 enzymes: SgfI * January 29, 2014 11:50 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 374 557 624 Size: 374 183 67 Fragments arranged by size: 374 183 67 AlwNI CAG_nnn'CTG Cuts at: 0 225 397 624 Size: 225 172 227 Fragments arranged by size: 227 225 172 ApaI G_GGCC'C Cuts at: 0 415 624 Size: 415 209 AscI GG'CGCG_CC Cuts at: 0 254 624 Size: 254 370 AvaI C'yCGr_G Cuts at: 0 78 357 373 529 583 624 Size: 78 279 16 156 54 41 Fragments arranged by size: 279 156 78 54 41 16 BanI G'GyrC_C Cuts at: 0 348 624 Size: 348 276 BanII G_rGCy'C Cuts at: 0 415 424 584 590 624 Size: 415 9 160 6 34 Fragments arranged by size: 415 160 34 9 6 BbvCI CC'TCA_GC Cuts at: 0 476 624 Size: 476 148 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 338 372 624 Size: 338 34 252 Fragments arranged by size: 338 252 34 BglI GCCn_nnn'nGGC Cuts at: 0 419 624 Size: 419 205 Bme1580I G_kGCm'C Cuts at: 0 240 415 624 Size: 240 175 209 Fragments arranged by size: 240 209 175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 580 624 Size: 580 44 Bpu10I CC'TnA_GC Cuts at: 0 476 624 Size: 476 148 BsaAI yAC'GTr Cuts at: 0 285 624 Size: 285 339 BsaHI Gr'CG_yC Cuts at: 0 340 624 Size: 340 284 BsaWI w'CCGG_w Cuts at: 0 498 570 624 Size: 498 72 54 Fragments arranged by size: 498 72 54 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 7 37 624 Size: 7 30 587 Fragments arranged by size: 587 30 7 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 466 624 Size: 466 158 BseYI C'CCAG_C Cuts at: 0 239 624 Size: 239 385 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 624 Size: 214 410 BsiEI CG_ry'CG Cuts at: 0 22 39 624 Size: 22 17 585 Fragments arranged by size: 585 22 17 BsiHKAI G_wGCw'C Cuts at: 0 119 624 Size: 119 505 BsmBI CGTCTCn'nnnn_ Cuts at: 0 55 624 Size: 55 569 Bsp1286I G_dGCh'C Cuts at: 0 119 240 415 424 584 590 624 Size: 119 121 175 9 160 6 34 Fragments arranged by size: 175 160 121 119 34 9 6 BsrBI CCG'CTC Cuts at: 0 18 45 624 Size: 18 27 579 Fragments arranged by size: 579 27 18 BssHII G'CGCG_C Cuts at: 0 254 624 Size: 254 370 BstAPI GCAn_nnn'nTGC Cuts at: 0 145 624 Size: 145 479 BstXI CCAn_nnnn'nTGG Cuts at: 0 335 624 Size: 335 289 Bsu36I CC'TnA_GG Cuts at: 0 416 624 Size: 416 208 BtgI C'CryG_G Cuts at: 0 248 624 Size: 248 376 BtsI GCAGTG_nn' Cuts at: 0 148 624 Size: 148 476 EaeI y'GGCC_r Cuts at: 0 19 624 Size: 19 605 EagI C'GGCC_G Cuts at: 0 19 624 Size: 19 605 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 88 624 Size: 88 536 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 374 557 580 624 Size: 374 183 23 44 Fragments arranged by size: 374 183 44 23 EcoO109I rG'GnC_Cy Cuts at: 0 411 412 443 624 Size: 411 1 31 181 Fragments arranged by size: 411 181 31 1 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 7 39 624 Size: 7 32 585 Fragments arranged by size: 585 32 7 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 71 624 Size: 71 553 MspA1I CmG'CkG Cuts at: 0 250 624 Size: 250 374 NspI r_CATG'y Cuts at: 0 371 624 Size: 371 253 PfoI T'CCnGG_A Cuts at: 0 439 624 Size: 439 185 PmlI CAC'GTG Cuts at: 0 285 624 Size: 285 339 PpuMI rG'GwC_Cy Cuts at: 0 443 624 Size: 443 181 PspOMI G'GGCC_C Cuts at: 0 411 624 Size: 411 213 PstI C_TGCA'G Cuts at: 0 71 624 Size: 71 553 SacII CC_GC'GG Cuts at: 0 251 624 Size: 251 373 SanDI GG'GwC_CC Cuts at: 0 443 624 Size: 443 181 SfcI C'TryA_G Cuts at: 0 67 624 Size: 67 557 SmaI CCC'GGG Cuts at: 0 375 624 Size: 375 249 StuI AGG'CCT Cuts at: 0 481 624 Size: 481 143 XmaI C'CCGG_G Cuts at: 0 373 624 Size: 373 251 Enzymes that do cut: AcuI AlwNI ApaI AscI AvaI BanI BanII BbvCI BcgI BglI Bme1580I BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BssHII BstAPI BstXI Bsu36I BtgI BtsI EaeI EagI EciI Eco57MI EcoO109I Hin4I MmeI MspA1I NspI PfoI PmlI PpuMI PspOMI PstI SacII SanDI SfcI SmaI StuI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI BsaBI BsiWI BsmI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI