################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI AocI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbsI BbvCI BcgI BciVI BclI BfrI BlnI BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tsp45I Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 316 GTCTAC GT^[AC][GT]AC AcsI 1322 GAATTC [GA]^AATT[CT] Alw44I 716 GTGCAC G^TGCAC AlwI 1305 GGATC GGATC(4/5) ApaLI 716 GTGCAC G^TGCAC ApoI 1322 GAATTC [GA]^AATT[CT] BalI 137 TGGCCA TGG^CCA BbeI 923 GGCGCC GGCGC^C BfaI 659 CTAG C^TAG BglII 795 AGATCT A^GATCT BsgI 421 GTGCAG GTGCAG(16/14) BsrBI 574 CCGCTC CCGCTC(-3/-3) BstAPI 1413 GCACGTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 795 AGATCT [GA]^GATC[CT] DraIII 719 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 636 CCGTGG C^C[GA][CT]GG EaeI 137 TGGCCA [CT]^GGCC[GA] EcoRI 1322 GAATTC G^AATTC FauI 370 GCGGG CCCGC(4/6) HphI 842 TCACC GGTGA(8/7) KasI 923 GGCGCC G^GCGCC MaeI 659 CTAG C^TAG MaeIII 1001 GTAAC ^GT[ACGT]AC MflI 795 AGATCT [GA]^GATC[CT] MluNI 137 TGGCCA TGG^CCA MscI 137 TGGCCA TGG^CCA MslI 538 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 923 GGCGCC GG^CGCC NspI 735 GCATGT [GA]CATG^[CT] PaeR7I 959 CTCGAG C^TCGAG SfoI 923 GGCGCC GGC^GCC SmlI 959 CTCGAG C^T[CT][GA]AG StuI 404 AGGCCT AGG^CCT Tsp509I 1323 AATT ^AATT XcmI 342 CCACACCTTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 959 CTCGAG C^TCGAG XhoII 795 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Aor51HI 1296 AGCGCT AGC^GCT 1362 AGCGCT BanI 923 GGCGCC G^G[CT][GA]CC 1257 GGTGCC BbrPI 745 CACGTG CAC^GTG 1414 CACGTG BlpI 181 GCTGAGC GC^T[ACGT]AGC 1358 GCTCAGC Bpu1102I 181 GCTGAGC GC^T[ACGT]AGC 1358 GCTCAGC BsaAI 745 CACGTG [CT]AC^GT[GA] 1414 CACGTG BsaI 548 GAGACC GGTCTC(1/5) 853 GAGACC CelII 181 GCTGAGC GC^T[ACGT]AGC 1358 GCTCAGC Eco47III 1296 AGCGCT AGC^GCT 1362 AGCGCT EcoNI 75 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 417 CCTCGTGCAGG EcoT14I 194 CCAAGG C^C[AT][AT]GG 552 CCAAGG NlaIII 736 CATG CATG^ 1176 CATG PleI 101 GACTC GAGTC(4/5) 804 GAGTC PmaCI 745 CACGTG CAC^GTG 1414 CACGTG PmlI 745 CACGTG CAC^GTG 1414 CACGTG PpuMI 755 GGGACCC [GA]G^G[AT]CC[CT] 1071 GGGACCC PstI 143 CTGCAG CTGCA^G 1366 CTGCAG SacI 1356 GAGCTC GAGCT^C 1383 GAGCTC SfcI 143 CTGCAG C^T[GA][CT]AG 1366 CTGCAG SfiI 405 GGCCTTCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1087 GGCCGAGGGGGCC SmaI 28 CCCGGG CCC^GGG 1067 CCCGGG StyI 194 CCAAGG C^C[AT][AT]GG 552 CCAAGG TaqI 788 TCGA T^CGA 960 TCGA TthHB8I 788 TCGA T^CGA 960 TCGA XmaCI 28 CCCGGG C^CCGGG 1067 CCCGGG XmaI 28 CCCGGG C^CCGGG 1067 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AcyI AfaI AluI AlwNI ApaI AspHI AvaI AvaII BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BssSI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI Ksp632I MaeII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIV PflMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI TfiI TseI TspRI Van91I