(Circular) (Six-base) MAPSORT of: pFN21AE1325.seq Check: 593 from: 1 to: 6211 pFN21AE1325 6211 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4021 278 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2322 3554 1937 Size: 385 1232 4594 Fragments arranged by size: 4594 1232 385 Acc65I G'GTAC_C Cuts at: 3532 3532 Size: 6211 AclI AA'CG_TT Cuts at: 1563 4338 4711 1563 Size: 2775 373 3063 Fragments arranged by size: 3063 2775 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2258 4274 5658 5926 1632 Size: 626 2016 1384 268 1917 Fragments arranged by size: 2016 1917 1384 626 268 AfeI AGC'GCT Cuts at: 3303 3369 3303 Size: 66 6145 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6014 AflIII A'CryG_T Cuts at: 1203 5110 6087 1203 Size: 3907 977 1327 Fragments arranged by size: 3907 1327 977 AgeI A'CCGG_T Cuts at: 5042 5042 Size: 6211 AhdI GACnn_n'nnGTC Cuts at: 4940 4940 Size: 6211 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6211 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6211 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2057 2089 2057 Size: 32 6179 AlwNI CAG_nnn'CTG Cuts at: 2305 2657 3128 3954 5526 2305 Size: 352 471 826 1572 2990 Fragments arranged by size: 2990 1572 826 471 352 ApaI G_GGCC'C Cuts at: 2018 2422 3104 3355 3412 2018 Size: 404 682 251 57 4817 Fragments arranged by size: 4817 682 404 251 57 ApaLI G'TGCA_C Cuts at: 2721 4268 5424 2721 Size: 1547 1156 3508 Fragments arranged by size: 3508 1547 1156 ApoI r'AATT_y Cuts at: 1493 3327 3520 3746 3898 6007 1493 Size: 1834 193 226 152 2109 1697 Fragments arranged by size: 2109 1834 1697 226 193 152 AseI AT'TA_AT Cuts at: 160 1032 4765 160 Size: 872 3733 1606 Fragments arranged by size: 3733 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 AvaI C'yCGr_G Cuts at: 1870 1943 2033 2964 3072 3536 1870 Size: 73 90 931 108 464 4545 Fragments arranged by size: 4545 931 464 108 90 73 BamHI G'GATC_C Cuts at: 3541 3908 3541 Size: 367 5844 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2928 3262 3532 4987 618 Size: 325 217 627 1141 334 270 1455 1842 Fragments arranged by size: 1842 1455 1141 627 334 325 270 217 BanII G_rGCy'C Cuts at: 1391 2018 2422 2526 2649 3104 3355 3365 3392 Size: 627 404 104 123 455 251 10 27 Cuts at: 3392 3412 3530 1391 Size: 20 118 4072 Fragments arranged by size: 4072 627 455 404 251 123 118 104 27 20 10 BbeI G_GCGC'C Cuts at: 2932 2932 Size: 6211 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5240 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4402 4436 1903 Size: 34 2465 34 3678 Fragments arranged by size: 3678 2465 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4103 5313 4103 Size: 1210 5001 BclI T'GATC_A Cuts at: 1548 1548 Size: 6211 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2268 2417 3099 Size: 107 122 71 631 605 596 149 682 Cuts at: 3099 4822 136 Size: 1723 1525 Fragments arranged by size: 1723 1525 682 631 605 596 149 122 107 71 BglII A'GATC_T Cuts at: 2800 6206 2800 Size: 3406 2805 BlpI GC'TnA_GC Cuts at: 2187 3364 3628 2187 Size: 1177 264 4770 Fragments arranged by size: 4770 1177 264 Bme1580I G_kGCm'C Cuts at: 1790 2018 2422 2725 3104 3355 3412 4272 5428 Size: 228 404 303 379 251 57 860 1156 Cuts at: 5428 1790 Size: 2573 Fragments arranged by size: 2573 1156 860 404 379 303 251 228 57 BmgBI CAC'GTC Cuts at: 1318 2782 1318 Size: 1464 4747 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2721 3942 4900 Size: 416 53 83 399 24 1304 1221 958 Cuts at: 4900 442 Size: 1753 Fragments arranged by size: 1753 1304 1221 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6211 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2326 2540 2326 Size: 214 5997 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2202 2379 2553 2803 2924 3105 3183 Size: 830 133 177 174 250 121 181 78 Cuts at: 3183 4871 1239 Size: 1688 2579 Fragments arranged by size: 2579 1688 830 250 181 177 174 133 121 78 Bpu10I CC'TnA_GC Cuts at: 2686 2686 Size: 6211 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4336 5201 5499 4336 Size: 865 298 5048 Fragments arranged by size: 5048 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2547 2852 4874 882 Size: 617 1048 305 2022 2219 Fragments arranged by size: 2219 2022 1048 617 305 BsaAI yAC'GTr Cuts at: 493 1204 2752 3421 4002 493 Size: 711 1548 669 581 2702 Fragments arranged by size: 2702 1548 711 669 581 BsaBI GATnn'nnATC Cuts at: 6205 6205 Size: 6211 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2473 2853 2929 4018 Size: 53 83 186 936 940 380 76 1089 Cuts at: 4018 4400 5035 275 Size: 382 635 1451 Fragments arranged by size: 1451 1089 940 936 635 382 380 186 83 76 53 BsaWI w'CCGG_w Cuts at: 4644 5042 5316 5463 5874 4644 Size: 398 274 147 411 4981 Fragments arranged by size: 4981 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2057 2087 1178 Size: 30 165 30 654 30 5302 Fragments arranged by size: 5302 654 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2058 2072 2532 2982 3442 1186 Size: 872 14 460 450 460 3955 Fragments arranged by size: 3955 872 460 460 450 14 BseYI C'CCAG_C Cuts at: 2650 2668 2841 3215 5414 5910 2650 Size: 18 173 374 2199 496 2951 Fragments arranged by size: 2951 2199 496 374 173 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2447 1163 Size: 573 711 4927 Fragments arranged by size: 4927 711 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3661 4422 5450 1540 Size: 275 186 1660 761 1028 2301 Fragments arranged by size: 2301 1660 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2050 2193 2725 3365 3392 4272 4357 5428 2050 Size: 143 532 640 27 880 85 1071 2833 Fragments arranged by size: 2833 1071 880 640 532 143 85 27 BsmI GAATG_Cn' Cuts at: 3722 3815 3722 Size: 93 6118 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2018 2050 2193 2422 2526 2649 2725 Size: 399 228 32 143 229 104 123 76 Cuts at: 2725 3104 3355 3365 3392 3412 3530 4272 4357 Size: 379 251 10 27 20 118 742 85 Cuts at: 4357 5428 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 379 251 229 228 143 123 118 104 85 76 32 27 20 10 BspEI T'CCGG_A Cuts at: 5874 5874 Size: 6211 BspHI T'CATG_A Cuts at: 4100 5830 4100 Size: 1730 4481 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3566 844 Size: 2722 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2581 4098 1394 Size: 429 758 1517 3507 Fragments arranged by size: 3507 1517 758 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4706 4880 65 Size: 1183 3458 174 1396 Fragments arranged by size: 3458 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4855 5042 1700 Size: 110 3045 187 2869 Fragments arranged by size: 3045 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6211 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6211 BssSI C'ACGA_G Cuts at: 2093 2423 2626 4271 5283 2093 Size: 330 203 1645 1012 3021 Fragments arranged by size: 3021 1645 1012 330 203 BstAPI GCAn_nnn'nTGC Cuts at: 794 3424 794 Size: 2630 3581 BstBI TT'CG_AA Cuts at: 5015 5015 Size: 6211 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6211 BstYI r'GATC_y Cuts at: 1972 2800 3541 3908 4293 4310 5762 5848 5860 Size: 828 741 367 385 17 1452 86 12 Cuts at: 5860 6206 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 828 741 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2641 513 Size: 552 1576 4083 Fragments arranged by size: 4083 1576 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3495 487 Size: 683 56 2269 3203 Fragments arranged by size: 3203 2269 683 56 BtsI GCAGTG_nn' Cuts at: 2145 3026 3143 3727 4521 4541 2145 Size: 881 117 584 794 20 3815 Fragments arranged by size: 3815 881 794 584 117 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6211 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2134 2169 529 Size: 35 1570 35 4571 Fragments arranged by size: 4571 1570 35 35 DraI TTT'AAA Cuts at: 3515 3515 Size: 6211 DraIII CAC_nnn'GTG Cuts at: 2729 2729 Size: 6211 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5218 809 Size: 507 3902 1802 Fragments arranged by size: 3902 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 3658 4547 Size: 54 1425 50 275 18 312 1516 889 Cuts at: 4547 6176 8 Size: 1629 43 Fragments arranged by size: 1629 1516 1425 889 312 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3658 1537 Size: 275 1846 4090 Fragments arranged by size: 4090 1846 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2502 2509 3425 4141 1353 Size: 1149 7 916 716 3423 Fragments arranged by size: 3423 1149 916 716 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2249 2934 4798 5172 5318 387 Size: 1064 138 660 685 1864 374 146 1280 Fragments arranged by size: 1864 1280 1064 685 660 374 146 138 EcoICRI GAG'CTC Cuts at: 3363 3390 3363 Size: 27 6184 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2202 2258 2379 2553 2803 2924 Size: 393 437 133 56 121 174 250 121 Cuts at: 2924 3105 3183 4274 4871 5658 5926 1239 Size: 181 78 1091 597 787 268 1524 Fragments arranged by size: 1524 1091 787 597 437 393 268 250 181 174 133 121 121 78 56 EcoNI CCTnn'n_nnAGG Cuts at: 2084 2426 2084 Size: 342 5869 EcoO109I rG'GnC_Cy Cuts at: 2014 2419 2564 2761 3077 3100 3101 3274 3351 Size: 405 145 197 316 23 1 173 77 Cuts at: 3351 3352 3408 3409 2014 Size: 1 56 1 4816 Fragments arranged by size: 4816 405 316 197 173 145 77 56 23 1 1 1 EcoRI G'AATT_C Cuts at: 3327 3520 3898 3327 Size: 193 378 5640 Fragments arranged by size: 5640 378 193 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2269 2269 Size: 6211 FspI TGC'GCA Cuts at: 4717 4717 Size: 6211 HaeII r_GCGC'y Cuts at: 1678 2174 2932 3305 3371 5358 1678 Size: 496 758 373 66 1987 2531 Fragments arranged by size: 2531 1987 758 496 373 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4852 4884 4926 4958 1373 Size: 32 135 32 3280 32 42 32 2626 Fragments arranged by size: 3280 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3555 3801 669 Size: 988 281 1617 246 3079 Fragments arranged by size: 3079 1617 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6211 HpaI GTT'AAC Cuts at: 3801 3801 Size: 6211 KasI G'GCGC_C Cuts at: 2928 2928 Size: 6211 KpnI G_GTAC'C Cuts at: 3536 3536 Size: 6211 MfeI C'AATT_G Cuts at: 3810 3810 Size: 6211 MluI A'CGCG_T Cuts at: 6087 6087 Size: 6211 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3480 5325 5509 1305 Size: 2175 1845 184 2007 Fragments arranged by size: 2175 2007 1845 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 10 Size: 54 1425 343 312 4077 Fragments arranged by size: 4077 1425 343 312 54 MslI CAynn'nnrTG Cuts at: 518 1166 2547 4169 4528 4687 518 Size: 648 1381 1622 359 159 2042 Fragments arranged by size: 2042 1622 1381 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2054 2161 2672 3334 3627 4304 5452 5697 Size: 448 107 511 662 293 677 1148 245 Cuts at: 5697 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 662 511 448 293 245 107 NarI GG'CG_CC Cuts at: 2929 2929 Size: 6211 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5659 NdeI CA'TA_TG Cuts at: 387 387 Size: 6211 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6211 NotI GC'GGCC_GC Cuts at: 3658 3658 Size: 6211 NspI r_CATG'y Cuts at: 1132 1229 2744 3569 5114 1132 Size: 97 1515 825 1545 2229 Fragments arranged by size: 2229 1545 1515 825 97 PasI CC'CwG_GG Cuts at: 1348 2587 3347 1348 Size: 1239 760 4212 Fragments arranged by size: 4212 1239 760 PciI A'CATG_T Cuts at: 5110 5110 Size: 6211 PflMI CCAn_nnn'nTGG Cuts at: 1108 2657 3128 3161 1108 Size: 1549 471 33 4158 Fragments arranged by size: 4158 1549 471 33 PfoI T'CCnGG_A Cuts at: 2085 3221 2085 Size: 1136 5075 PmeI GTTT'AAAC Cuts at: 3515 3515 Size: 6211 PmlI CAC'GTG Cuts at: 2752 3421 2752 Size: 669 5542 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2057 2089 4280 4312 5793 5825 2057 Size: 32 2191 32 1481 32 2443 Fragments arranged by size: 2443 2191 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2761 3077 2761 Size: 316 5895 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6211 PsiI TTA'TAA Cuts at: 3781 3781 Size: 6211 PspOMI G'GGCC_C Cuts at: 2014 2418 3100 3351 3408 2014 Size: 404 682 251 57 4817 Fragments arranged by size: 4817 682 404 251 57 PstI C_TGCA'G Cuts at: 830 2152 3375 3563 830 Size: 1322 1223 188 3478 Fragments arranged by size: 3478 1322 1223 188 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6211 PvuII CAG'CTG Cuts at: 2161 2672 3334 2161 Size: 511 662 5038 Fragments arranged by size: 5038 662 511 SacI G_AGCT'C Cuts at: 3365 3392 3365 Size: 27 6184 SalI G'TCGA_C Cuts at: 1936 3553 1936 Size: 1617 4594 SanDI GG'GwC_CC Cuts at: 2761 3077 2761 Size: 316 5895 SbfI CC_TGCA'GG Cuts at: 3563 3563 Size: 6211 ScaI AGT'ACT Cuts at: 4459 6171 4459 Size: 1712 4499 SfcI C'TryA_G Cuts at: 826 1045 2148 3371 3559 4694 5375 5566 826 Size: 219 1103 1223 188 1135 681 191 1471 Fragments arranged by size: 1471 1223 1135 1103 681 219 191 188 SfiI GGCCn_nnn'nGGCC Cuts at: 2417 3099 2417 Size: 682 5529 SfoI GGC'GCC Cuts at: 2930 2930 Size: 6211 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6211 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6211 SmaI CCC'GGG Cuts at: 1872 2035 3074 3538 1872 Size: 163 1039 464 4545 Fragments arranged by size: 4545 1039 464 163 SmlI C'TyrA_G Cuts at: 820 1017 1943 2964 4315 5216 5478 820 Size: 197 926 1021 1351 901 262 1553 Fragments arranged by size: 1553 1351 1021 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4002 493 Size: 3509 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6211 SphI G_CATG'C Cuts at: 1132 3569 1132 Size: 2437 3774 SspI AAT'ATT Cuts at: 5 52 4135 5 Size: 47 4083 2081 Fragments arranged by size: 4083 2081 47 StuI AGG'CCT Cuts at: 1517 2411 1517 Size: 894 5317 StyI C'CwwG_G Cuts at: 513 1065 2199 2557 3650 513 Size: 552 1134 358 1093 3074 Fragments arranged by size: 3074 1134 1093 552 358 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2740 3193 4238 4255 4408 4593 5038 Size: 1255 1160 453 1045 17 153 185 445 Cuts at: 5038 325 Size: 1498 Fragments arranged by size: 1498 1255 1160 1045 453 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2307 4457 6169 Size: 274 80 33 51 1443 330 2150 1712 Cuts at: 6169 96 Size: 138 Fragments arranged by size: 2150 1712 1443 330 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2766 2798 6171 6203 2766 Size: 32 3373 32 2774 Fragments arranged by size: 3373 2774 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6211 XbaI T'CTAG_A Cuts at: 3547 3547 Size: 6211 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2354 2354 Size: 6211 XhoI C'TCGA_G Cuts at: 1943 2964 1943 Size: 1021 5190 XmaI C'CCGG_G Cuts at: 1870 2033 3072 3536 1870 Size: 163 1039 464 4545 Fragments arranged by size: 4545 1039 464 163 XmnI GAAnn'nnTTC Cuts at: 4340 4340 Size: 6211 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4019 276 Size: 53 83 186 936 2485 2468 Fragments arranged by size: 2485 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SapI SexAI SrfI SwaI