(Linear) (Six-base) MAPSORT of: KIEE1325.seq Check: 301 from: 1 to: 1506 KIEE1325.orf With 182 enzymes: SgfI * July 15, 2010 18:36 .. AccI GT'mk_AC Cuts at: 0 317 1506 Size: 317 1189 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 1506 Size: 253 1253 AfeI AGC'GCT Cuts at: 0 1298 1364 1506 Size: 1298 66 142 Fragments arranged by size: 1298 142 66 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 52 84 1506 Size: 52 32 1422 Fragments arranged by size: 1422 52 32 AlwNI CAG_nnn'CTG Cuts at: 0 300 652 1123 1506 Size: 300 352 471 383 Fragments arranged by size: 471 383 352 300 ApaI G_GGCC'C Cuts at: 0 13 417 1099 1350 1407 1506 Size: 13 404 682 251 57 99 Fragments arranged by size: 682 404 251 99 57 13 ApaLI G'TGCA_C Cuts at: 0 716 1506 Size: 716 790 ApoI r'AATT_y Cuts at: 0 1322 1506 Size: 1322 184 AvaI C'yCGr_G Cuts at: 0 28 959 1067 1506 Size: 28 931 108 439 Fragments arranged by size: 931 439 108 28 BanI G'GyrC_C Cuts at: 0 923 1257 1506 Size: 923 334 249 Fragments arranged by size: 923 334 249 BanII G_rGCy'C Cuts at: 0 13 417 521 644 1099 1350 1360 1387 Size: 13 404 104 123 455 251 10 27 Cuts at: 1387 1407 1506 Size: 20 99 Fragments arranged by size: 455 404 251 123 104 99 27 20 13 10 BbeI G_GCGC'C Cuts at: 0 927 1506 Size: 927 579 BglI GCCn_nnn'nGGC Cuts at: 0 263 412 1094 1506 Size: 263 149 682 412 Fragments arranged by size: 682 412 263 149 BglII A'GATC_T Cuts at: 0 795 1506 Size: 795 711 BlpI GC'TnA_GC Cuts at: 0 182 1359 1506 Size: 182 1177 147 Fragments arranged by size: 1177 182 147 Bme1580I G_kGCm'C Cuts at: 0 13 417 720 1099 1350 1407 1506 Size: 13 404 303 379 251 57 99 Fragments arranged by size: 404 379 303 251 99 57 13 BmgBI CAC'GTC Cuts at: 0 777 1506 Size: 777 729 BmrI ACTGGGnnnn_n' Cuts at: 0 716 1506 Size: 716 790 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 321 535 1506 Size: 321 214 971 Fragments arranged by size: 971 321 214 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 197 374 548 798 919 1100 1178 Size: 64 133 177 174 250 121 181 78 Cuts at: 1178 1506 Size: 328 Fragments arranged by size: 328 250 181 177 174 133 121 78 64 Bpu10I CC'TnA_GC Cuts at: 0 681 1506 Size: 681 825 BsaI GGTCTCn'nnnn_ Cuts at: 0 542 847 1506 Size: 542 305 659 Fragments arranged by size: 659 542 305 BsaAI yAC'GTr Cuts at: 0 747 1416 1506 Size: 747 669 90 Fragments arranged by size: 747 669 90 BsaHI Gr'CG_yC Cuts at: 0 468 848 924 1506 Size: 468 380 76 582 Fragments arranged by size: 582 468 380 76 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 52 82 1506 Size: 52 30 1424 Fragments arranged by size: 1424 52 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 53 67 527 977 1437 1506 Size: 53 14 460 450 460 69 Fragments arranged by size: 460 460 450 69 53 14 BseYI C'CCAG_C Cuts at: 0 645 663 836 1210 1506 Size: 645 18 173 374 296 Fragments arranged by size: 645 374 296 173 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 442 1506 Size: 442 1064 BsiHKAI G_wGCw'C Cuts at: 0 45 188 720 1360 1387 1506 Size: 45 143 532 640 27 119 Fragments arranged by size: 640 532 143 119 45 27 Bsp1286I G_dGCh'C Cuts at: 0 13 45 188 417 521 644 720 1099 Size: 13 32 143 229 104 123 76 379 Cuts at: 1099 1350 1360 1387 1407 1506 Size: 251 10 27 20 99 Fragments arranged by size: 379 251 229 143 123 104 99 76 32 27 20 13 10 BsrBI CCG'CTC Cuts at: 0 576 1506 Size: 576 930 BssSI C'ACGA_G Cuts at: 0 88 418 621 1506 Size: 88 330 203 885 Fragments arranged by size: 885 330 203 88 BstAPI GCAn_nnn'nTGC Cuts at: 0 1419 1506 Size: 1419 87 BstYI r'GATC_y Cuts at: 0 795 1506 Size: 795 711 BtgI C'CryG_G Cuts at: 0 636 1506 Size: 636 870 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1490 1506 Size: 1490 16 BtsI GCAGTG_nn' Cuts at: 0 140 1021 1138 1506 Size: 140 881 117 368 Fragments arranged by size: 881 368 140 117 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 129 164 1506 Size: 129 35 1342 Fragments arranged by size: 1342 129 35 DraIII CAC_nnn'GTG Cuts at: 0 724 1506 Size: 724 782 EaeI y'GGCC_r Cuts at: 0 137 1506 Size: 137 1369 EarI CTCTTCn'nnn_ Cuts at: 0 497 504 1420 1506 Size: 497 7 916 86 Fragments arranged by size: 916 497 86 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 244 929 1506 Size: 244 685 577 Fragments arranged by size: 685 577 244 EcoICRI GAG'CTC Cuts at: 0 1358 1385 1506 Size: 1358 27 121 Fragments arranged by size: 1358 121 27 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 64 197 253 374 548 798 919 1100 Size: 64 133 56 121 174 250 121 181 Cuts at: 1100 1178 1506 Size: 78 328 Fragments arranged by size: 328 250 181 174 133 121 121 78 64 56 EcoNI CCTnn'n_nnAGG Cuts at: 0 79 421 1506 Size: 79 342 1085 Fragments arranged by size: 1085 342 79 EcoO109I rG'GnC_Cy Cuts at: 0 9 414 559 756 1072 1095 1096 1269 Size: 9 405 145 197 316 23 1 173 Cuts at: 1269 1346 1347 1403 1404 1506 Size: 77 1 56 1 102 Fragments arranged by size: 405 316 197 173 145 102 77 56 23 9 1 1 1 EcoRI G'AATT_C Cuts at: 0 1322 1506 Size: 1322 184 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 264 1506 Size: 264 1242 HaeII r_GCGC'y Cuts at: 0 169 927 1300 1366 1506 Size: 169 758 373 66 140 Fragments arranged by size: 758 373 169 140 66 KasI G'GCGC_C Cuts at: 0 923 1506 Size: 923 583 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1475 1506 Size: 1475 31 MscI TGG'CCA Cuts at: 0 139 1506 Size: 139 1367 MslI CAynn'nnrTG Cuts at: 0 542 1506 Size: 542 964 MspA1I CmG'CkG Cuts at: 0 49 156 667 1329 1506 Size: 49 107 511 662 177 Fragments arranged by size: 662 511 177 107 49 NarI GG'CG_CC Cuts at: 0 924 1506 Size: 924 582 NspI r_CATG'y Cuts at: 0 739 1506 Size: 739 767 PasI CC'CwG_GG Cuts at: 0 582 1342 1506 Size: 582 760 164 Fragments arranged by size: 760 582 164 PflMI CCAn_nnn'nTGG Cuts at: 0 652 1123 1156 1506 Size: 652 471 33 350 Fragments arranged by size: 652 471 350 33 PfoI T'CCnGG_A Cuts at: 0 80 1216 1506 Size: 80 1136 290 Fragments arranged by size: 1136 290 80 PmlI CAC'GTG Cuts at: 0 747 1416 1506 Size: 747 669 90 Fragments arranged by size: 747 669 90 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 52 84 1506 Size: 52 32 1422 Fragments arranged by size: 1422 52 32 PpuMI rG'GwC_Cy Cuts at: 0 756 1072 1506 Size: 756 316 434 Fragments arranged by size: 756 434 316 PspOMI G'GGCC_C Cuts at: 0 9 413 1095 1346 1403 1506 Size: 9 404 682 251 57 103 Fragments arranged by size: 682 404 251 103 57 9 PstI C_TGCA'G Cuts at: 0 147 1370 1506 Size: 147 1223 136 Fragments arranged by size: 1223 147 136 PvuII CAG'CTG Cuts at: 0 156 667 1329 1506 Size: 156 511 662 177 Fragments arranged by size: 662 511 177 156 SacI G_AGCT'C Cuts at: 0 1360 1387 1506 Size: 1360 27 119 Fragments arranged by size: 1360 119 27 SanDI GG'GwC_CC Cuts at: 0 756 1072 1506 Size: 756 316 434 Fragments arranged by size: 756 434 316 SfcI C'TryA_G Cuts at: 0 143 1366 1506 Size: 143 1223 140 Fragments arranged by size: 1223 143 140 SfiI GGCCn_nnn'nGGCC Cuts at: 0 412 1094 1506 Size: 412 682 412 Fragments arranged by size: 682 412 412 SfoI GGC'GCC Cuts at: 0 925 1506 Size: 925 581 SmaI CCC'GGG Cuts at: 0 30 1069 1506 Size: 30 1039 437 Fragments arranged by size: 1039 437 30 SmlI C'TyrA_G Cuts at: 0 959 1506 Size: 959 547 StuI AGG'CCT Cuts at: 0 406 1506 Size: 406 1100 StyI C'CwwG_G Cuts at: 0 194 552 1506 Size: 194 358 954 Fragments arranged by size: 954 358 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 735 1188 1506 Size: 735 453 318 Fragments arranged by size: 735 453 318 TatI w'GTAC_w Cuts at: 0 302 1506 Size: 302 1204 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 761 793 1506 Size: 761 32 713 Fragments arranged by size: 761 713 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 349 1506 Size: 349 1157 XhoI C'TCGA_G Cuts at: 0 959 1506 Size: 959 547 XmaI C'CCGG_G Cuts at: 0 28 1067 1506 Size: 28 1039 439 Fragments arranged by size: 1039 439 28 Enzymes that do cut: AccI AcuI AfeI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI BssSI BstAPI BstYI BtgI BtgZI BtsI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII KasI MmeI MscI MslI MspA1I NarI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SanDI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BmtI BpuEI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI DraI DrdI EagI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XmnI ZraI