(Circular) (Six-base) MAPSORT of: pFN21AE6114.seq Check: 3142 from: 1 to: 5206 pFN21AE6114 5206 bp With 182 enzymes: SgfI * December 8, 2014 13:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3016 278 Size: 53 83 186 936 1480 2468 Fragments arranged by size: 2468 1480 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2549 1937 Size: 612 4594 Acc65I G'GTAC_C Cuts at: 2527 2527 Size: 5206 AclI AA'CG_TT Cuts at: 1563 3333 3706 1563 Size: 1770 373 3063 Fragments arranged by size: 3063 1770 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 3269 4653 4921 1632 Size: 695 942 1384 268 1917 Fragments arranged by size: 1917 1384 942 695 268 AfeI AGC'GCT Cuts at: 2192 2192 Size: 5206 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5009 AflIII A'CryG_T Cuts at: 1203 4105 5082 1203 Size: 2902 977 1327 Fragments arranged by size: 2902 1327 977 AgeI A'CCGG_T Cuts at: 4037 4037 Size: 5206 AhdI GACnn_n'nnGTC Cuts at: 3935 3935 Size: 5206 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5206 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5206 AlwNI CAG_nnn'CTG Cuts at: 2182 2350 2949 4521 2182 Size: 168 599 1572 2867 Fragments arranged by size: 2867 1572 599 168 ApaLI G'TGCA_C Cuts at: 3263 4419 3263 Size: 1156 4050 ApoI r'AATT_y Cuts at: 1493 2515 2741 2893 5002 1493 Size: 1022 226 152 2109 1697 Fragments arranged by size: 2109 1697 1022 226 152 AseI AT'TA_AT Cuts at: 160 1032 3760 160 Size: 872 2728 1606 Fragments arranged by size: 2728 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5206 AvaI C'yCGr_G Cuts at: 1870 1943 2105 2531 1870 Size: 73 162 426 4545 Fragments arranged by size: 4545 426 162 73 BamHI G'GATC_C Cuts at: 2536 2903 2536 Size: 367 4839 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 2434 2453 2527 3982 Size: 325 217 627 516 131 19 74 1455 Cuts at: 3982 618 Size: 1842 Fragments arranged by size: 1842 1455 627 516 325 217 131 74 19 BanII G_rGCy'C Cuts at: 1391 2525 1391 Size: 1134 4072 BbeI G_GCGC'C Cuts at: 2307 2307 Size: 5206 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4235 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3397 3431 1903 Size: 34 1460 34 3678 Fragments arranged by size: 3678 1460 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3098 4308 3098 Size: 1210 3996 BclI T'GATC_A Cuts at: 1548 1548 Size: 5206 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2069 3817 136 Size: 107 122 71 631 605 397 1748 1525 Fragments arranged by size: 1748 1525 631 605 397 122 107 71 BglII A'GATC_T Cuts at: 5201 5201 Size: 5206 BlpI GC'TnA_GC Cuts at: 2021 2623 2021 Size: 602 4604 Bme1580I G_kGCm'C Cuts at: 1790 2458 3267 4423 1790 Size: 668 809 1156 2573 Fragments arranged by size: 2573 1156 809 668 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5206 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2937 3895 442 Size: 416 53 83 399 24 1520 958 1753 Fragments arranged by size: 1753 1520 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5206 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2134 2134 Size: 5206 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 3866 1239 Size: 1142 1485 2579 Fragments arranged by size: 2579 1485 1142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3331 4196 4494 3331 Size: 865 298 4043 Fragments arranged by size: 4043 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2144 3869 882 Size: 617 645 1725 2219 Fragments arranged by size: 2219 1725 645 617 BsaAI yAC'GTr Cuts at: 493 1204 2997 493 Size: 711 1793 2702 Fragments arranged by size: 2702 1793 711 BsaBI GATnn'nnATC Cuts at: 2219 5200 2219 Size: 2981 2225 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2304 3013 3395 4030 Size: 53 83 186 936 771 709 382 635 Cuts at: 4030 275 Size: 1451 Fragments arranged by size: 1451 936 771 709 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3639 4037 4311 4458 4869 3639 Size: 398 274 147 411 3976 Fragments arranged by size: 3976 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4981 Fragments arranged by size: 4981 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2123 2144 2330 1186 Size: 868 69 21 186 4062 Fragments arranged by size: 4062 868 186 69 21 BseYI C'CCAG_C Cuts at: 4409 4905 4409 Size: 496 4710 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2207 1163 Size: 573 471 4162 Fragments arranged by size: 4162 573 471 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2230 2656 3417 4445 1540 Size: 275 186 229 426 761 1028 2301 Fragments arranged by size: 2301 1028 761 426 275 229 186 BsiHKAI G_wGCw'C Cuts at: 3267 3352 4423 3267 Size: 85 1071 4050 Fragments arranged by size: 4050 1071 85 BsmI GAATG_Cn' Cuts at: 2717 2810 2717 Size: 93 5113 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2458 2525 3267 3352 4423 1391 Size: 399 668 67 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 668 399 85 67 BspEI T'CCGG_A Cuts at: 4869 4869 Size: 5206 BspHI T'CATG_A Cuts at: 3095 4825 3095 Size: 1730 3476 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2561 844 Size: 1717 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2478 3093 1394 Size: 429 655 615 3507 Fragments arranged by size: 3507 655 615 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3701 3875 65 Size: 1183 2453 174 1396 Fragments arranged by size: 2453 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3850 4037 1700 Size: 110 2040 187 2869 Fragments arranged by size: 2869 2040 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5206 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5206 BssSI C'ACGA_G Cuts at: 3266 4278 3266 Size: 1012 4194 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5206 BstBI TT'CG_AA Cuts at: 4010 4010 Size: 5206 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5206 BstYI r'GATC_y Cuts at: 1972 2083 2536 2903 3288 3305 4757 4843 4855 Size: 111 453 367 385 17 1452 86 12 Cuts at: 4855 5201 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 453 385 367 346 111 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2230 513 Size: 552 939 226 3489 Fragments arranged by size: 3489 939 552 226 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4467 Fragments arranged by size: 4467 683 56 BtsI GCAGTG_nn' Cuts at: 2177 2722 3516 3536 2177 Size: 545 794 20 3847 Fragments arranged by size: 3847 794 545 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5206 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5171 DraI TTT'AAA Cuts at: 2510 2510 Size: 5206 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4213 809 Size: 507 2897 1802 Fragments arranged by size: 2897 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2227 2377 2653 Size: 54 1425 50 275 18 397 150 276 Cuts at: 2653 3542 5171 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 397 276 275 150 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2227 2653 1537 Size: 275 415 426 4090 Fragments arranged by size: 4090 426 415 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3136 1353 Size: 1783 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3793 4167 4313 387 Size: 1064 138 2204 374 146 1280 Fragments arranged by size: 2204 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2381 3269 3866 4653 4921 1239 Size: 393 695 54 888 597 787 268 1524 Fragments arranged by size: 1524 888 787 695 597 393 268 54 EcoNI CCTnn'n_nnAGG Cuts at: 2126 2126 Size: 5206 EcoRI G'AATT_C Cuts at: 2515 2893 2515 Size: 378 4828 FspI TGC'GCA Cuts at: 3712 3712 Size: 5206 HaeII r_GCGC'y Cuts at: 1678 2056 2194 2307 2360 4353 1678 Size: 378 138 113 53 1993 2531 Fragments arranged by size: 2531 1993 378 138 113 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3847 3879 3921 3953 1373 Size: 32 135 32 2275 32 42 32 2626 Fragments arranged by size: 2626 2275 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2550 2796 669 Size: 988 281 612 246 3079 Fragments arranged by size: 3079 988 612 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5206 HpaI GTT'AAC Cuts at: 2796 2796 Size: 5206 KasI G'GCGC_C Cuts at: 2303 2303 Size: 5206 KpnI G_GTAC'C Cuts at: 2531 2531 Size: 5206 MfeI C'AATT_G Cuts at: 2805 2805 Size: 5206 MluI A'CGCG_T Cuts at: 5082 5082 Size: 5206 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2016 4320 4504 1305 Size: 711 2304 184 2007 Fragments arranged by size: 2304 2007 711 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2379 10 Size: 54 1425 343 547 2837 Fragments arranged by size: 2837 1425 547 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3164 3523 3682 518 Size: 648 1998 359 159 2042 Fragments arranged by size: 2042 1998 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2251 2263 2275 2422 2622 3299 4447 4692 Size: 645 12 12 147 200 677 1148 245 Cuts at: 4692 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 645 245 200 147 12 12 NarI GG'CG_CC Cuts at: 2304 2304 Size: 5206 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3715 Fragments arranged by size: 3715 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5206 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5206 NotI GC'GGCC_GC Cuts at: 2653 2653 Size: 5206 NspI r_CATG'y Cuts at: 1132 1229 2564 4109 1132 Size: 97 1335 1545 2229 Fragments arranged by size: 2229 1545 1335 97 PasI CC'CwG_GG Cuts at: 1348 2371 1348 Size: 1023 4183 PciI A'CATG_T Cuts at: 4105 4105 Size: 5206 PflMI CCAn_nnn'nTGG Cuts at: 1108 2182 2386 2401 1108 Size: 1074 204 15 3913 Fragments arranged by size: 3913 1074 204 15 PmeI GTTT'AAAC Cuts at: 2510 2510 Size: 5206 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3275 3307 4788 4820 3275 Size: 32 1481 32 3661 Fragments arranged by size: 3661 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5206 PsiI TTA'TAA Cuts at: 2776 2776 Size: 5206 PstI C_TGCA'G Cuts at: 830 2241 2558 830 Size: 1411 317 3478 Fragments arranged by size: 3478 1411 317 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5206 PvuII CAG'CTG Cuts at: 2251 2422 2251 Size: 171 5035 SalI G'TCGA_C Cuts at: 1936 2548 1936 Size: 612 4594 SbfI CC_TGCA'GG Cuts at: 2558 2558 Size: 5206 ScaI AGT'ACT Cuts at: 3454 5166 3454 Size: 1712 3494 SfcI C'TryA_G Cuts at: 826 1045 2237 2554 3689 4370 4561 826 Size: 219 1192 317 1135 681 191 1471 Fragments arranged by size: 1471 1192 1135 681 317 219 191 SfoI GGC'GCC Cuts at: 2305 2305 Size: 5206 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5206 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5206 SmaI CCC'GGG Cuts at: 1872 2533 1872 Size: 661 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3310 4211 4473 820 Size: 197 926 1367 901 262 1553 Fragments arranged by size: 1553 1367 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2997 493 Size: 2504 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5206 SphI G_CATG'C Cuts at: 1132 2564 1132 Size: 1432 3774 SspI AAT'ATT Cuts at: 5 52 3130 5 Size: 47 3078 2081 Fragments arranged by size: 3078 2081 47 StuI AGG'CCT Cuts at: 1517 2063 1517 Size: 546 4660 StyI C'CwwG_G Cuts at: 513 1065 2004 2310 2645 513 Size: 552 939 306 335 3074 Fragments arranged by size: 3074 939 552 335 306 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2153 2363 3233 3250 3403 3588 4033 Size: 1255 573 210 870 17 153 185 445 Cuts at: 4033 325 Size: 1498 Fragments arranged by size: 1498 1255 870 573 445 210 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3452 5164 96 Size: 274 80 33 51 1443 1475 1712 138 Fragments arranged by size: 1712 1475 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5166 5198 5166 Size: 32 5174 Tth111I GACn'n_nGTC Cuts at: 1316 2092 1316 Size: 776 4430 XbaI T'CTAG_A Cuts at: 2542 2542 Size: 5206 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5206 XmaI C'CCGG_G Cuts at: 1870 2531 1870 Size: 661 4545 XmnI GAAnn'nnTTC Cuts at: 3335 3335 Size: 5206 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3014 276 Size: 53 83 186 936 1480 2468 Fragments arranged by size: 2468 1480 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI