################################## # NO CUT ENZYMES ( 186 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcsI AcyI AfaI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp509I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AflIII 105 ACATGT A^C[GA][CT]GT BanI 302 GGCACC G^G[CT][GA]CC BanII 156 GGGCTC G[GA]GC[CT]^C BbsI 213 GAAGAC GAAGAC(2/6) BbvI 261 GCTGC GCAGC(8/12) BglII 164 AGATCT A^GATCT BmyI 156 GGGCTC G[AGT]GC[ACT]^C BpmI 310 CTCCAG CTGGAG(16/14) BpuAI 213 GAAGAC GAAGAC(2/6) BsaI 382 GAGACC GGTCTC(1/5) BsaWI 110 TCCGGT [AT]^CCGG[AT] BsgI 133 GTGCAG GTGCAG(16/14) Bsp1286I 156 GGGCTC G[AGT]GC[ACT]^C BspLU11I 105 ACATGT A^CATGT BstF5I 219 CATCC GGATG(2/0) BstXI 326 CCAGCTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 164 AGATCT [GA]^GATC[CT] DsaI 175 CCACGG C^C[GA][CT]GG EarI 466 CTCTTC CTCTTC(1/4) EcoNI 306 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 122 CCAAGG C^C[AT][AT]GG Fnu4HI 261 GCTGC GC^[ACGT]GC FokI 219 CATCC GGATG(9/13) HapII 111 CCGG C^CGG HinfI 280 GAGTC G^A[ACGT]TC HpaII 111 CCGG C^CGG ItaI 261 GCTGC GC^[ACGT]GC Ksp632I 466 CTCTTC CTCTTC(1/4) MaeIII 394 GTGAC ^GT[ACGT]AC MflI 164 AGATCT [GA]^GATC[CT] MspI 111 CCGG C^CGG MwoI 4 GCTCCCAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 77 CATATG CA^TATG PleI 280 GAGTC GAGTC(4/5) SexAI 140 ACCAGGT A^CC[AT]GGT StyI 122 CCAAGG C^C[AT][AT]GG TaqI 356 TCGA T^CGA TseI 261 GCTGC G^C[AT]GC Tsp45I 394 GTGAC ^GT[GC]AC TthHB8I 356 TCGA T^CGA XhoII 164 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AluI 260 AGCT AG^CT 328 AGCT AvaII 138 GGACC G^G[AT]CC 323 GGTCC BseRI 307 CTCCTC GAGGAG(10/8) 256 GAGGAG BsrDI 349 GCAATG GCAATG(2/0) 401 GCAATG BsrI 414 ACTGG ACTGG(1/-1) 248 CCAGT Cfr13I 138 GGACC G^G[ACGT]CC 323 GGTCC DpnII 165 GATC ^GATC 354 GATC HphI 240 GGTGA GGTGA(8/7) 286 GGTGA MboI 165 GATC ^GATC 354 GATC MseI 55 TTAA T^TAA 169 TTAA NdeII 165 GATC ^GATC 354 GATC Sau3AI 165 GATC ^GATC 354 GATC Sau96I 138 GGACC G^G[ACGT]CC 323 GGTCC SfaNI 67 GATGC GCATC(5/9) 446 GCATC Tru9I 55 TTAA T^TAA 169 TTAA TspRI 249 CAGTG CA[GC]TG[ACGT][ACGT]^ 332 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### BsaJI BsiYI BslI BsmAI BssKI BstNI DdeI EcoRII MboII MnlI MvaI NlaIII NlaIV NspI ScrFI