################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AccIII AclI AflII AflIII AhdI Alw44I ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbeI BbrPI BciVI BclI BfrI BglII BlnI BsaAI BsaBI BsaI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BssHII Bst1107I BstEII BstPI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI HincII HindII HindIII HpaI KasI MamI MfeI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PflFI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Tru9I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 2086 GACGTC GACGT^C Acc65I 1019 GGTACC G^GTACC AccI 1921 GTCTAC GT^[AC][GT]AC AcsI 294 GAATTT [GA]^AATT[CT] AgeI 1016 ACCGGT A^CCGGT Aor51HI 1559 AGCGCT AGC^GCT ApaI 2156 GGGCCC GGGCC^C ApoI 294 GAATTT [GA]^AATT[CT] Asp718I 1019 GGTACC G^GTACC AvaI 1695 CTCGGG C^[CT]CG[GA]G BalI 1376 TGGCCA TGG^CCA BcgI 1805 GCAGCTTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 1258 CTAG C^TAG BglI 1348 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsoBI 1695 CTCGGG C^[CT]CG[GA]G Bsp1407I 171 TGTACA T^GTACA BspMI 1022 ACCTGC ACCTGC(4/8) BsrDI 1942 CATTGC GCAATG(2/0) BsrFI 1016 ACCGGT [GA]^CCGG[CT] BsrGI 171 TGTACA T^GTACA BssSI 505 CACGAG CACGAG(-5/-1) BstAPI 1067 GCATCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 298 TTCGAA TT^CGAA Cfr10I 1016 ACCGGT [GA]^CCGG[CT] DrdI 693 GACAGATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1559 AGCGCT AGC^GCT EcoNI 624 CCTGTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 2310 ATGCAT ATGCA^T KpnI 1019 GGTACC GGTAC^C KspI 1042 CCGCGG CCGC^GG MaeI 1258 CTAG C^TAG MluNI 1376 TGGCCA TGG^CCA MscI 1376 TGGCCA TGG^CCA NsiI 2310 ATGCAT ATGCA^T NspI 220 GCATGT [GA]CATG^[CT] NspV 298 TTCGAA TT^CGAA PinAI 1016 ACCGGT A^CCGGT PshAI 1254 GACGCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 532 CTGCAG CTGCA^G SacI 1306 GAGCTC GAGCT^C SacII 1042 CCGCGG CCGC^GG SapI 450 GCTCTTC GCTCTTC(1/4) SfuI 298 TTCGAA TT^CGAA SspBI 171 TGTACA T^GTACA Tsp509I 295 AATT ^AATT XcmI 2129 CCACAAGCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 367 CGCG CG^CG 1043 CGCG AcyI 1498 GACGCC G[GA]^CG[CT]C 2086 GACGTC AocI 513 CCTCAGG CC^T[ACGT]AGG 2096 CCTCAGG Asp700I 969 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1482 GAAGTGCTTC AspHI 115 GTGCTC G[AT]GC[AT]^C 1306 GAGCTC BanII 1306 GAGCTC G[GA]GC[CT]^C 2156 GGGCCC BbvCI 1656 CCTCAGC CCTCAGC(-5/-2) 1683 CCTCAGC BlpI 412 GCTGAGC GC^T[ACGT]AGC 1590 GCTGAGC Bpu1102I 412 GCTGAGC GC^T[ACGT]AGC 1590 GCTGAGC BsaHI 1498 GACGCC G[GA]^CG[CT]C 2086 GACGTC BsaWI 1016 ACCGGT [AT]^CCGG[AT] 1432 ACCGGA BsiHKAI 115 GTGCTC G[AT]GC[AT]^C 1306 GAGCTC BstUI 367 CGCG CG^CG 1043 CGCG BstXI 1223 CCACTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2048 CCAAACCCCTGG Bsu36I 513 CCTCAGG CC^T[ACGT]AGG 2096 CCTCAGG CelII 412 GCTGAGC GC^T[ACGT]AGC 1590 GCTGAGC EarI 451 CTCTTC CTCTTC(1/4) 1582 GAAGAG Eco81I 513 CCTCAGG CC^T[ACGT]AGG 2096 CCTCAGG EcoT14I 1199 CCTTGG C^C[AT][AT]GG 1505 CCAAGG HaeII 1559 AGCGCT [GA]GCGC^[CT] 2013 GGCGCT Hin1I 1498 GACGCC G[GA]^CG[CT]C 2086 GACGTC Ksp632I 451 CTCTTC CTCTTC(1/4) 1582 GAAGAG MaeII 1883 ACGT A^CGT 2087 ACGT MslI 205 CACGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1224 CACTGTGGTG MvnI 367 CGCG CG^CG 1043 CGCG PflMI 209 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1056 CCAGCTGCTGG SexAI 191 ACCTGGT A^CC[AT]GGT 1166 ACCTGGT SmlI 96 CTTGAG C^T[CT][GA]AG 1467 CTTGAG StuI 342 AGGCCT AGG^CCT 389 AGGCCT StyI 1199 CCTTGG C^C[AT][AT]GG 1505 CCAAGG Van91I 209 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1056 CCAGCTGCTGG XmnI 969 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1482 GAAGTGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanI BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoII