(Linear) (Six-base) MAPSORT of: KIEE4521.seq Check: 5442 from: 1 to: 1671 KIEE4521.orf With 182 enzymes: SgfI * March 22, 2012 18:15 .. AatII G_ACGT'C Cuts at: 0 368 1671 Size: 368 1303 AccI GT'mk_AC Cuts at: 0 368 1337 1671 Size: 368 969 334 Fragments arranged by size: 969 368 334 Acc65I G'GTAC_C Cuts at: 0 1257 1540 1671 Size: 1257 283 131 Fragments arranged by size: 1257 283 131 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 531 1671 Size: 531 1140 AflIII A'CryG_T Cuts at: 0 1340 1671 Size: 1340 331 AleI CACnn'nnGTG Cuts at: 0 382 819 1369 1671 Size: 382 437 550 302 Fragments arranged by size: 550 437 382 302 AlwNI CAG_nnn'CTG Cuts at: 0 182 1671 Size: 182 1489 ApaI G_GGCC'C Cuts at: 0 47 1671 Size: 47 1624 ApoI r'AATT_y Cuts at: 0 573 659 1671 Size: 573 86 1012 Fragments arranged by size: 1012 573 86 AvaI C'yCGr_G Cuts at: 0 942 1671 Size: 942 729 BanI G'GyrC_C Cuts at: 0 153 228 1257 1450 1540 1671 Size: 153 75 1029 193 90 131 Fragments arranged by size: 1029 193 153 131 90 75 BanII G_rGCy'C Cuts at: 0 47 469 1671 Size: 47 422 1202 Fragments arranged by size: 1202 422 47 BciVI GTATCCnnnnn_n' Cuts at: 0 1370 1671 Size: 1370 301 BclI T'GATC_A Cuts at: 0 884 1671 Size: 884 787 BfrBI ATG'CAT Cuts at: 0 718 1671 Size: 718 953 BglII A'GATC_T Cuts at: 0 560 620 1671 Size: 560 60 1051 Fragments arranged by size: 1051 560 60 BlpI GC'TnA_GC Cuts at: 0 73 1671 Size: 73 1598 Bme1580I G_kGCm'C Cuts at: 0 47 233 1671 Size: 47 186 1438 Fragments arranged by size: 1438 186 47 BmgBI CAC'GTC Cuts at: 0 306 1671 Size: 306 1365 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 343 1671 Size: 310 33 1328 Fragments arranged by size: 1328 310 33 Bpu10I CC'TnA_GC Cuts at: 0 172 1671 Size: 172 1499 BsaAI yAC'GTr Cuts at: 0 1341 1671 Size: 1341 330 BsaHI Gr'CG_yC Cuts at: 0 161 365 1671 Size: 161 204 1306 Fragments arranged by size: 1306 204 161 BsaWI w'CCGG_w Cuts at: 0 339 1019 1671 Size: 339 680 652 Fragments arranged by size: 680 652 339 BseYI C'CCAG_C Cuts at: 0 347 1152 1671 Size: 347 805 519 Fragments arranged by size: 805 519 347 BsiEI CG_ry'CG Cuts at: 0 222 1461 1671 Size: 222 1239 210 Fragments arranged by size: 1239 222 210 BsiHKAI G_wGCw'C Cuts at: 0 143 1671 Size: 143 1528 Bsp1286I G_dGCh'C Cuts at: 0 47 143 233 469 1671 Size: 47 96 90 236 1202 Fragments arranged by size: 1202 236 96 90 47 BspEI T'CCGG_A Cuts at: 0 339 1019 1671 Size: 339 680 652 Fragments arranged by size: 680 652 339 BsrFI r'CCGG_y Cuts at: 0 103 251 1671 Size: 103 148 1420 Fragments arranged by size: 1420 148 103 BsrGI T'GTAC_A Cuts at: 0 1343 1671 Size: 1343 328 BstAPI GCAn_nnn'nTGC Cuts at: 0 1188 1671 Size: 1188 483 BstBI TT'CG_AA Cuts at: 0 1005 1671 Size: 1005 666 BstEII G'GTnAC_C Cuts at: 0 18 1026 1671 Size: 18 1008 645 Fragments arranged by size: 1008 645 18 BstXI CCAn_nnnn'nTGG Cuts at: 0 633 1065 1671 Size: 633 432 606 Fragments arranged by size: 633 606 432 BstYI r'GATC_y Cuts at: 0 560 620 996 1671 Size: 560 60 376 675 Fragments arranged by size: 675 560 376 60 BtgI C'CryG_G Cuts at: 0 527 1205 1331 1671 Size: 527 678 126 340 Fragments arranged by size: 678 527 340 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 249 1153 1671 Size: 249 904 518 Fragments arranged by size: 904 518 249 BtsI GCAGTG_nn' Cuts at: 0 202 1211 1239 1671 Size: 202 1009 28 432 Fragments arranged by size: 1009 432 202 28 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 918 953 1671 Size: 918 35 718 Fragments arranged by size: 918 718 35 EaeI y'GGCC_r Cuts at: 0 219 501 1208 1671 Size: 219 282 707 463 Fragments arranged by size: 707 463 282 219 EagI C'GGCC_G Cuts at: 0 219 1671 Size: 219 1452 EarI CTCTTCn'nnn_ Cuts at: 0 866 1225 1249 1671 Size: 866 359 24 422 Fragments arranged by size: 866 422 359 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 783 1671 Size: 783 888 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 310 343 531 1671 Size: 310 33 188 1140 Fragments arranged by size: 1140 310 188 33 EcoO109I rG'GnC_Cy Cuts at: 0 43 44 423 1671 Size: 43 1 379 1248 Fragments arranged by size: 1248 379 43 1 HaeII r_GCGC'y Cuts at: 0 57 80 182 283 787 1200 1671 Size: 57 23 102 101 504 413 471 Fragments arranged by size: 504 471 413 102 101 57 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 836 868 1671 Size: 836 32 803 Fragments arranged by size: 836 803 32 KpnI G_GTAC'C Cuts at: 0 1261 1544 1671 Size: 1261 283 127 Fragments arranged by size: 1261 283 127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 650 1671 Size: 650 1021 MscI TGG'CCA Cuts at: 0 1210 1671 Size: 1210 461 MslI CAynn'nnrTG Cuts at: 0 382 532 819 1033 1063 1117 1369 1384 Size: 382 150 287 214 30 54 252 15 Cuts at: 1384 1480 1618 1671 Size: 96 138 53 Fragments arranged by size: 382 287 252 214 150 138 96 54 53 30 15 MspA1I CmG'CkG Cuts at: 0 146 239 321 486 1254 1671 Size: 146 93 82 165 768 417 Fragments arranged by size: 768 417 165 146 93 82 NcoI C'CATG_G Cuts at: 0 527 1205 1331 1671 Size: 527 678 126 340 Fragments arranged by size: 678 527 340 126 NdeI CA'TA_TG Cuts at: 0 1158 1671 Size: 1158 513 NsiI A_TGCA'T Cuts at: 0 720 1671 Size: 720 951 NspI r_CATG'y Cuts at: 0 537 1671 Size: 537 1134 PspOMI G'GGCC_C Cuts at: 0 43 1671 Size: 43 1628 PstI C_TGCA'G Cuts at: 0 560 1671 Size: 560 1111 PvuII CAG'CTG Cuts at: 0 239 321 486 1254 1671 Size: 239 82 165 768 417 Fragments arranged by size: 768 417 239 165 82 SexAI A'CCwGG_T Cuts at: 0 398 1671 Size: 398 1273 SfcI C'TryA_G Cuts at: 0 556 1283 1671 Size: 556 727 388 Fragments arranged by size: 727 556 388 SphI G_CATG'C Cuts at: 0 537 1671 Size: 537 1134 SspI AAT'ATT Cuts at: 0 686 1186 1671 Size: 686 500 485 Fragments arranged by size: 686 500 485 StyI C'CwwG_G Cuts at: 0 527 1205 1331 1446 1671 Size: 527 678 126 115 225 Fragments arranged by size: 678 527 225 126 115 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 403 442 1216 1372 1671 Size: 403 39 774 156 299 Fragments arranged by size: 774 403 299 156 39 TatI w'GTAC_w Cuts at: 0 1343 1671 Size: 1343 328 XmnI GAAnn'nnTTC Cuts at: 0 1126 1671 Size: 1126 545 ZraI GAC'GTC Cuts at: 0 366 1671 Size: 366 1305 Enzymes that do cut: AatII AccI Acc65I AcuI AflIII AleI AlwNI ApaI ApoI AvaI BanI BanII BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I KpnI MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI PspOMI PstI PvuII SexAI SfcI SphI SspI StyI TaqII TatI XmnI ZraI Enzymes that do not cut: AarI AclI AfeI AflII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BglI BmrI BmtI BplI BpuEI BsaI BsaBI BsaXI BseRI BsgI BsiWI BsmI BsmBI BspHI BspMI BsrBI BsrDI BssHII BssSI BstZ17I Bsu36I ClaI DraI DraIII DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI MfeI MluI NaeI NarI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI