################################## # NO CUT ENZYMES ( 217 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI KspI MaeII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI TseI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AcsI 309 AAATTT [GA]^AATT[CT] AfaI 57 GTAC GT^AC AhdI 33 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 309 AAATTT [GA]^AATT[CT] AspEI 33 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BspMI 93 GCAGGT ACCTGC(4/8) DdeI 163 CTCAG C^T[ACGT]AG DrdI 142 GACAAAGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 33 GACCAACAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 205 GAAGAG CTCTTC(1/4) HinfI 159 GACTC G^A[ACGT]TC HphI 361 GGTGA GGTGA(8/7) Ksp632I 205 GAAGAG CTCTTC(1/4) MaeIII 106 GTGAC ^GT[ACGT]AC MboII 205 GAAGA GAAGA(8/7) MnlI 7 GAGG CCTC(7/6) MseI 326 TTAA T^TAA MspA1I 196 CAGCTG C[AC]G^C[GT]G MwoI 10 GCTTTCTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 60 CATG CATG^ PleI 159 GACTC GAGTC(4/5) PstI 224 CTGCAG CTGCA^G PvuII 196 CAGCTG CAG^CTG RsaI 57 GTAC GT^AC SfcI 224 CTGCAG C^T[GA][CT]AG Tru9I 326 TTAA T^TAA Tsp45I 106 GTGAC ^GT[GC]AC TspRI 222 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 86 CCACTAGGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### AluI 29 AGCT AG^CT 197 AGCT BfaI 70 CTAG C^TAG 89 CTAG BsmAI 140 GAGAC GTCTC(1/5) 266 GAGAC BssKI 23 CCTGG ^CC[ACGT]GG 258 CCTGG BstNI 23 CCTGG CC^[AT]GG 258 CCTGG EcoRII 23 CCTGG ^CC[AT]GG 258 CCTGG MaeI 70 CTAG C^TAG 89 CTAG MvaI 23 CCTGG CC^[AT]GG 258 CCTGG ScrFI 23 CCTGG CC^[ACGT]GG 258 CCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 3 enzymes) # ######################################### BstF5I FokI Tsp509I