(Circular) (Six-base) MAPSORT of: pFN21AE6724.seq Check: 8171 from: 1 to: 6784 pFN21AE6724 6784 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4594 278 Size: 53 83 186 936 3058 2468 Fragments arranged by size: 3058 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2475 2745 3949 4127 1937 Size: 538 270 1204 178 4594 Fragments arranged by size: 4594 1204 538 270 178 Acc65I G'GTAC_C Cuts at: 4105 4105 Size: 6784 AclI AA'CG_TT Cuts at: 1563 2981 3672 4911 5284 1563 Size: 1418 691 1239 373 3063 Fragments arranged by size: 3063 1418 1239 691 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2274 2417 2598 2633 3024 3375 4847 6231 Size: 642 143 181 35 391 351 1472 1384 Cuts at: 6231 6499 1632 Size: 268 1917 Fragments arranged by size: 1917 1472 1384 642 391 351 268 181 143 35 AflII C'TTAA_G Cuts at: 820 1017 2054 820 Size: 197 1037 5550 Fragments arranged by size: 5550 1037 197 AflIII A'CryG_T Cuts at: 1203 2411 3588 5683 6660 1203 Size: 1208 1177 2095 977 1327 Fragments arranged by size: 2095 1327 1208 1177 977 AgeI A'CCGG_T Cuts at: 5615 5615 Size: 6784 AhdI GACnn_n'nnGTC Cuts at: 2997 5513 2997 Size: 2516 4268 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6784 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6784 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2193 2225 2193 Size: 32 6752 AlwNI CAG_nnn'CTG Cuts at: 3326 4527 6099 3326 Size: 1201 1572 4011 Fragments arranged by size: 4011 1572 1201 ApaLI G'TGCA_C Cuts at: 2357 2807 4841 5997 2357 Size: 450 2034 1156 3144 Fragments arranged by size: 3144 2034 1156 450 ApoI r'AATT_y Cuts at: 1493 2163 2310 2617 2877 3264 4093 4319 4471 Size: 670 147 307 260 387 829 226 152 Cuts at: 4471 6580 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 829 670 387 307 260 226 152 147 AseI AT'TA_AT Cuts at: 160 1032 5338 160 Size: 872 4306 1606 Fragments arranged by size: 4306 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6784 AvaI C'yCGr_G Cuts at: 1870 1943 4109 1870 Size: 73 2166 4545 Fragments arranged by size: 4545 2166 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3021 3054 3021 Size: 33 6751 BamHI G'GATC_C Cuts at: 4114 4481 4114 Size: 367 6417 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4105 5560 618 Size: 325 217 627 2318 1455 1842 Fragments arranged by size: 2318 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3195 4103 1391 Size: 1804 908 4072 Fragments arranged by size: 4072 1804 908 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5813 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4975 5009 1903 Size: 34 3038 34 3678 Fragments arranged by size: 3678 3038 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3022 4676 5886 3022 Size: 1654 1210 3920 Fragments arranged by size: 3920 1654 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6784 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5395 136 Size: 107 122 71 631 605 3723 1525 Fragments arranged by size: 3723 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3902 6779 3902 Size: 2877 3907 BlpI GC'TnA_GC Cuts at: 4201 4201 Size: 6784 Bme1580I G_kGCm'C Cuts at: 1790 2361 2811 4845 6001 1790 Size: 571 450 2034 1156 2573 Fragments arranged by size: 2573 2034 1156 571 450 BmgBI CAC'GTC Cuts at: 1318 2712 1318 Size: 1394 5390 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3631 4515 5473 Size: 416 53 83 399 24 2214 884 958 Cuts at: 5473 442 Size: 1753 Fragments arranged by size: 2214 1753 958 884 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6784 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2064 2250 3586 5444 1239 Size: 815 10 186 1336 1858 2579 Fragments arranged by size: 2579 1858 1336 815 186 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2696 4909 5774 6072 2696 Size: 2213 865 298 3408 Fragments arranged by size: 3408 2213 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2043 5447 882 Size: 617 544 3404 2219 Fragments arranged by size: 3404 2219 617 544 BsaAI yAC'GTr Cuts at: 493 1204 3589 4575 493 Size: 711 2385 986 2702 Fragments arranged by size: 2702 2385 986 711 BsaBI GATnn'nnATC Cuts at: 6778 6778 Size: 6784 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4591 4973 5608 275 Size: 53 83 186 936 3058 382 635 1451 Fragments arranged by size: 3058 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5217 5615 5889 6036 6447 5217 Size: 398 274 147 411 5554 Fragments arranged by size: 5554 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 2073 2103 2340 Size: 30 165 30 606 30 34 30 237 Cuts at: 2340 2370 2457 2487 3797 3827 1178 Size: 30 87 30 1310 30 4135 Fragments arranged by size: 4135 1310 606 237 165 87 34 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2061 3098 3227 1186 Size: 875 1037 129 4743 Fragments arranged by size: 4743 1037 875 129 BseYI C'CCAG_C Cuts at: 3818 5987 6483 3818 Size: 2169 496 4119 Fragments arranged by size: 4119 2169 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2174 2681 3454 3581 1163 Size: 573 438 507 773 127 4366 Fragments arranged by size: 4366 773 573 507 438 127 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4234 4995 6023 1540 Size: 275 186 2233 761 1028 2301 Fragments arranged by size: 2301 2233 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2110 2361 2566 2811 3195 3759 4845 4930 6001 Size: 251 205 245 384 564 1086 85 1071 Cuts at: 6001 2110 Size: 2893 Fragments arranged by size: 2893 1086 1071 564 384 251 245 205 85 BsmI GAATG_Cn' Cuts at: 2044 3517 3532 4295 4388 2044 Size: 1473 15 763 93 4440 Fragments arranged by size: 4440 1473 763 93 15 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2110 2361 2566 2811 3195 3759 4103 Size: 399 320 251 205 245 384 564 344 Cuts at: 4103 4845 4930 6001 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 564 399 384 344 320 251 245 205 85 BspEI T'CCGG_A Cuts at: 6447 6447 Size: 6784 BspHI T'CATG_A Cuts at: 3939 4673 6403 3939 Size: 734 1730 4320 Fragments arranged by size: 4320 1730 734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3961 4139 844 Size: 3117 178 3489 Fragments arranged by size: 3489 3117 178 BsrBI CCG'CTC Cuts at: 1394 1823 4671 1394 Size: 429 2848 3507 Fragments arranged by size: 3507 2848 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2799 3973 5279 5453 65 Size: 1183 1551 1174 1306 174 1396 Fragments arranged by size: 1551 1396 1306 1183 1174 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3838 5428 5615 1700 Size: 110 2028 1590 187 2869 Fragments arranged by size: 2869 2028 1590 187 110 BsrGI T'GTAC_A Cuts at: 96 2759 96 Size: 2663 4121 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6784 BssSI C'ACGA_G Cuts at: 4844 5856 4844 Size: 1012 5772 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6784 BstBI TT'CG_AA Cuts at: 3916 5588 3916 Size: 1672 5112 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6784 BstYI r'GATC_y Cuts at: 1972 3902 3972 4114 4481 4866 4883 6335 6421 Size: 1930 70 142 367 385 17 1452 86 Cuts at: 6421 6433 6779 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1930 1452 385 367 346 142 86 70 17 12 BstZ17I GTA'TAC Cuts at: 3950 3950 Size: 6784 BtgI C'CryG_G Cuts at: 513 1065 2004 2960 513 Size: 552 939 956 4337 Fragments arranged by size: 4337 956 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6045 Fragments arranged by size: 6045 683 56 BtsI GCAGTG_nn' Cuts at: 2650 4300 5094 5114 2650 Size: 1650 794 20 4320 Fragments arranged by size: 4320 1650 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6784 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6749 DraI TTT'AAA Cuts at: 4088 4088 Size: 6784 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5791 809 Size: 507 4475 1802 Fragments arranged by size: 4475 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2062 4231 5120 Size: 54 1425 50 275 18 232 2169 889 Cuts at: 5120 6749 8 Size: 1629 43 Fragments arranged by size: 2169 1629 1425 889 275 232 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4231 1537 Size: 275 2419 4090 Fragments arranged by size: 4090 2419 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2128 2850 3000 3288 3474 4714 1353 Size: 775 722 150 288 186 1240 3423 Fragments arranged by size: 3423 1240 775 722 288 186 150 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5371 5745 5891 387 Size: 1064 138 3782 374 146 1280 Fragments arranged by size: 3782 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3193 3193 Size: 6784 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2064 2250 2274 2417 2598 2633 Size: 393 422 10 186 24 143 181 35 Cuts at: 2633 3024 3375 3586 4847 5444 6231 6499 1239 Size: 391 351 211 1261 597 787 268 1524 Fragments arranged by size: 1524 1261 787 597 422 393 391 351 268 211 186 181 143 35 24 10 EcoNI CCTnn'n_nnAGG Cuts at: 2435 2435 Size: 6784 EcoO109I rG'GnC_Cy Cuts at: 2234 2428 2860 3323 3871 2234 Size: 194 432 463 548 5147 Fragments arranged by size: 5147 548 463 432 194 EcoRI G'AATT_C Cuts at: 2877 4093 4471 2877 Size: 1216 378 5190 Fragments arranged by size: 5190 1216 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2603 2603 Size: 6784 FspI TGC'GCA Cuts at: 5290 5290 Size: 6784 HaeII r_GCGC'y Cuts at: 1678 5931 1678 Size: 4253 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2073 2105 2983 3015 3611 Size: 32 135 32 501 32 878 32 596 Cuts at: 3611 3643 4037 4069 5425 5457 5499 5531 1373 Size: 32 394 32 1356 32 42 32 2626 Fragments arranged by size: 2626 1356 878 596 501 394 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3676 4128 4374 669 Size: 988 281 1738 452 246 3079 Fragments arranged by size: 3079 1738 988 452 281 246 HindIII A'AGCT_T Cuts at: 748 2870 748 Size: 2122 4662 HpaI GTT'AAC Cuts at: 3676 4374 3676 Size: 698 6086 KpnI G_GTAC'C Cuts at: 4109 4109 Size: 6784 MfeI C'AATT_G Cuts at: 4383 4383 Size: 6784 MluI A'CGCG_T Cuts at: 6660 6660 Size: 6784 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5898 6082 1305 Size: 4593 184 2007 Fragments arranged by size: 4593 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2064 10 Size: 54 1425 343 232 4730 Fragments arranged by size: 4730 1425 343 232 54 MslI CAynn'nnrTG Cuts at: 518 1166 2073 2399 2804 3180 3252 4742 5101 Size: 648 907 326 405 376 72 1490 359 Cuts at: 5101 5260 518 Size: 159 2042 Fragments arranged by size: 2042 1490 907 648 405 376 359 326 159 72 MspA1I CmG'CkG Cuts at: 1606 4200 4877 6025 6270 1606 Size: 2594 677 1148 245 2120 Fragments arranged by size: 2594 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5293 Fragments arranged by size: 5293 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6784 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6784 NotI GC'GGCC_GC Cuts at: 4231 4231 Size: 6784 NspI r_CATG'y Cuts at: 1132 1229 2415 3664 4142 5687 1132 Size: 97 1186 1249 478 1545 2229 Fragments arranged by size: 2229 1545 1249 1186 478 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6784 PciI A'CATG_T Cuts at: 2411 5683 2411 Size: 3272 3512 PflMI CCAn_nnn'nTGG Cuts at: 1108 3326 1108 Size: 2218 4566 PmeI GTTT'AAAC Cuts at: 4088 4088 Size: 6784 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4853 4885 6366 6398 4853 Size: 32 1481 32 5239 Fragments arranged by size: 5239 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2234 3323 3871 2234 Size: 1089 548 5147 Fragments arranged by size: 5147 1089 548 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6784 PsiI TTA'TAA Cuts at: 4354 4354 Size: 6784 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3910 3942 3910 Size: 32 6752 PstI C_TGCA'G Cuts at: 830 4136 830 Size: 3306 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6784 SacI G_AGCT'C Cuts at: 3195 3195 Size: 6784 SalI G'TCGA_C Cuts at: 1936 4126 1936 Size: 2190 4594 SanDI GG'GwC_CC Cuts at: 3871 3871 Size: 6784 SbfI CC_TGCA'GG Cuts at: 4136 4136 Size: 6784 ScaI AGT'ACT Cuts at: 3763 5032 6744 3763 Size: 1269 1712 3803 Fragments arranged by size: 3803 1712 1269 SfcI C'TryA_G Cuts at: 826 1045 3552 3580 3720 3978 4132 5267 5948 Size: 219 2507 28 140 258 154 1135 681 Cuts at: 5948 6139 826 Size: 191 1471 Fragments arranged by size: 2507 1471 1135 681 258 219 191 154 140 28 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6784 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6784 SmaI CCC'GGG Cuts at: 1872 4111 1872 Size: 2239 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2054 2675 4888 5789 6051 820 Size: 197 926 111 621 2213 901 262 1553 Fragments arranged by size: 2213 1553 926 901 621 262 197 111 SnaBI TAC'GTA Cuts at: 493 4575 493 Size: 4082 2702 SpeI A'CTAG_T Cuts at: 152 3929 152 Size: 3777 3007 SphI G_CATG'C Cuts at: 1132 4142 1132 Size: 3010 3774 SspI AAT'ATT Cuts at: 5 52 2283 3397 4708 5 Size: 47 2231 1114 1311 2081 Fragments arranged by size: 2231 2081 1311 1114 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6784 StyI C'CwwG_G Cuts at: 513 1065 2004 2172 3100 4223 513 Size: 552 939 168 928 1123 3074 Fragments arranged by size: 3074 1123 939 928 552 168 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2145 4811 4828 4981 5166 5611 325 Size: 1255 565 2666 17 153 185 445 1498 Fragments arranged by size: 2666 1498 1255 565 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2759 3761 5030 Size: 274 80 33 51 1443 782 1002 1269 Cuts at: 5030 6742 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1269 1002 782 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2340 2372 6744 6776 2340 Size: 32 4372 32 2348 Fragments arranged by size: 4372 2348 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6784 XbaI T'CTAG_A Cuts at: 4120 4120 Size: 6784 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2579 2579 Size: 6784 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6784 XmaI C'CCGG_G Cuts at: 1870 4109 1870 Size: 2239 4545 XmnI GAAnn'nnTTC Cuts at: 2618 3530 4913 2618 Size: 912 1383 4489 Fragments arranged by size: 4489 1383 912 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4592 276 Size: 53 83 186 936 3058 2468 Fragments arranged by size: 3058 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI