(Circular) (Six-base) MAPSORT of: pFN21AE1571.seq Check: 5884 from: 1 to: 5485 pFN21AE1571 5485 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3295 278 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2828 1937 Size: 891 4594 Acc65I G'GTAC_C Cuts at: 2806 2806 Size: 5485 AclI AA'CG_TT Cuts at: 1563 3612 3985 1563 Size: 2049 373 3063 Fragments arranged by size: 3063 2049 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2485 3548 4932 5200 1632 Size: 853 1063 1384 268 1917 Fragments arranged by size: 1917 1384 1063 853 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5288 AflIII A'CryG_T Cuts at: 1203 4384 5361 1203 Size: 3181 977 1327 Fragments arranged by size: 3181 1327 977 AgeI A'CCGG_T Cuts at: 4316 4316 Size: 5485 AhdI GACnn_n'nnGTC Cuts at: 4214 4214 Size: 5485 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5485 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2529 1184 Size: 1345 4140 AlwNI CAG_nnn'CTG Cuts at: 2410 2491 3228 4800 2410 Size: 81 737 1572 3095 Fragments arranged by size: 3095 1572 737 81 ApaI G_GGCC'C Cuts at: 2629 2629 Size: 5485 ApaLI G'TGCA_C Cuts at: 2353 3542 4698 2353 Size: 1189 1156 3140 Fragments arranged by size: 3140 1189 1156 ApoI r'AATT_y Cuts at: 1493 2794 3020 3172 5281 1493 Size: 1301 226 152 2109 1697 Fragments arranged by size: 2109 1697 1301 226 152 AseI AT'TA_AT Cuts at: 160 1032 4039 160 Size: 872 3007 1606 Fragments arranged by size: 3007 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 AvaI C'yCGr_G Cuts at: 1870 1943 2810 1870 Size: 73 867 4545 Fragments arranged by size: 4545 867 73 BamHI G'GATC_C Cuts at: 2815 3182 2815 Size: 367 5118 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2806 4261 618 Size: 325 217 627 1019 1455 1842 Fragments arranged by size: 1842 1455 1019 627 325 217 BanII G_rGCy'C Cuts at: 1391 2058 2107 2417 2629 2804 1391 Size: 667 49 310 212 175 4072 Fragments arranged by size: 4072 667 310 212 175 49 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2267 928 Size: 971 368 4146 Fragments arranged by size: 4146 971 368 BbvCI CC'TCA_GC Cuts at: 2424 2424 Size: 5485 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3676 3710 1903 Size: 34 1739 34 3678 Fragments arranged by size: 3678 1739 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3377 4587 3377 Size: 1210 4275 BclI T'GATC_A Cuts at: 1548 1548 Size: 5485 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4096 136 Size: 107 122 71 631 605 2424 1525 Fragments arranged by size: 2424 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5480 5480 Size: 5485 BlpI GC'TnA_GC Cuts at: 2902 2902 Size: 5485 Bme1580I G_kGCm'C Cuts at: 1790 2357 2481 2629 3546 4702 1790 Size: 567 124 148 917 1156 2573 Fragments arranged by size: 2573 1156 917 567 148 124 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5485 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2110 3216 4174 Size: 416 53 83 399 24 693 1106 958 Cuts at: 4174 442 Size: 1753 Fragments arranged by size: 1753 1106 958 693 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5485 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2052 2480 4145 1239 Size: 813 428 1665 2579 Fragments arranged by size: 2579 1665 813 428 Bpu10I CC'TnA_GC Cuts at: 2106 2411 2424 2106 Size: 305 13 5167 Fragments arranged by size: 5167 305 13 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3610 4475 4773 3610 Size: 865 298 4322 Fragments arranged by size: 4322 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2215 4148 882 Size: 617 716 1933 2219 Fragments arranged by size: 2219 1933 716 617 BsaAI yAC'GTr Cuts at: 493 1204 3276 493 Size: 711 2072 2702 Fragments arranged by size: 2702 2072 711 BsaBI GATnn'nnATC Cuts at: 5479 5479 Size: 5485 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3292 3674 4309 275 Size: 53 83 186 936 1759 382 635 1451 Fragments arranged by size: 1759 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2764 3918 4316 4590 4737 5148 2764 Size: 1154 398 274 147 411 3101 Fragments arranged by size: 3101 1154 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5260 Fragments arranged by size: 5260 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2735 2738 1186 Size: 1549 3 3933 Fragments arranged by size: 3933 1549 3 BseYI C'CCAG_C Cuts at: 2474 4688 5184 2474 Size: 2214 496 2775 Fragments arranged by size: 2775 2214 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2275 2359 2578 1163 Size: 573 539 84 219 4070 Fragments arranged by size: 4070 573 539 219 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2399 2935 3696 4724 1540 Size: 275 186 398 536 761 1028 2301 Fragments arranged by size: 2301 1028 761 536 398 275 186 BsiHKAI G_wGCw'C Cuts at: 2357 3546 3631 4702 2357 Size: 1189 85 1071 3140 Fragments arranged by size: 3140 1189 1071 85 BsmI GAATG_Cn' Cuts at: 2996 3089 2996 Size: 93 5392 BsmBI CGTCTCn'nnnn_ Cuts at: 2406 2406 Size: 5485 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2058 2107 2357 2417 2481 2629 2804 Size: 399 268 49 250 60 64 148 175 Cuts at: 2804 3546 3631 4702 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 268 250 175 148 85 64 60 49 BspEI T'CCGG_A Cuts at: 5148 5148 Size: 5485 BspHI T'CATG_A Cuts at: 3374 5104 3374 Size: 1730 3755 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2840 844 Size: 1996 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2393 3372 1394 Size: 429 570 979 3507 Fragments arranged by size: 3507 979 570 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2332 3980 4154 65 Size: 1183 1084 1648 174 1396 Fragments arranged by size: 1648 1396 1183 1084 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4129 4316 1700 Size: 110 2319 187 2869 Fragments arranged by size: 2869 2319 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5485 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5485 BssSI C'ACGA_G Cuts at: 2150 3545 4557 2150 Size: 1395 1012 3078 Fragments arranged by size: 3078 1395 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5485 BstBI TT'CG_AA Cuts at: 4289 4289 Size: 5485 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5485 BstXI CCAn_nnnn'nTGG Cuts at: 2022 2022 Size: 5485 BstYI r'GATC_y Cuts at: 1972 2549 2815 3182 3567 3584 5036 5122 5134 Size: 577 266 367 385 17 1452 86 12 Cuts at: 5134 5480 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 577 385 367 346 266 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3994 Fragments arranged by size: 3994 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4746 Fragments arranged by size: 4746 683 56 BtsI GCAGTG_nn' Cuts at: 2298 2342 3001 3795 3815 2298 Size: 44 659 794 20 3968 Fragments arranged by size: 3968 794 659 44 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5485 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5450 DraI TTT'AAA Cuts at: 2789 2789 Size: 5485 DraIII CAC_nnn'GTG Cuts at: 2361 2361 Size: 5485 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2395 4492 809 Size: 507 1079 2097 1802 Fragments arranged by size: 2097 1802 1079 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2012 2537 2619 Size: 54 1425 50 275 18 182 525 82 Cuts at: 2619 2932 3821 5450 8 Size: 313 889 1629 43 Fragments arranged by size: 1629 1425 889 525 313 275 182 82 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2932 1537 Size: 275 1120 4090 Fragments arranged by size: 4090 1120 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2376 3415 1353 Size: 1023 1039 3423 Fragments arranged by size: 3423 1039 1023 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4072 4446 4592 387 Size: 1064 138 2483 374 146 1280 Fragments arranged by size: 2483 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2052 2480 2485 3548 4145 4932 5200 Size: 393 420 428 5 1063 597 787 268 Cuts at: 5200 1239 Size: 1524 Fragments arranged by size: 1524 1063 787 597 428 420 393 268 5 EcoNI CCTnn'n_nnAGG Cuts at: 2153 2153 Size: 5485 EcoO109I rG'GnC_Cy Cuts at: 2043 2407 2625 2626 2043 Size: 364 218 1 4902 Fragments arranged by size: 4902 364 218 1 EcoRI G'AATT_C Cuts at: 2794 3172 2794 Size: 378 5107 FspI TGC'GCA Cuts at: 3991 3991 Size: 5485 HaeII r_GCGC'y Cuts at: 1678 4632 1678 Size: 2954 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4126 4158 4200 4232 1373 Size: 32 135 32 2554 32 42 32 2626 Fragments arranged by size: 2626 2554 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2829 3075 669 Size: 988 281 891 246 3079 Fragments arranged by size: 3079 988 891 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5485 HpaI GTT'AAC Cuts at: 3075 3075 Size: 5485 KpnI G_GTAC'C Cuts at: 2810 2810 Size: 5485 MfeI C'AATT_G Cuts at: 3084 3084 Size: 5485 MluI A'CGCG_T Cuts at: 5361 5361 Size: 5485 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4599 4783 1305 Size: 3294 184 2007 Fragments arranged by size: 3294 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2539 10 Size: 54 1425 343 707 2956 Fragments arranged by size: 2956 1425 707 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2582 3443 3802 3961 518 Size: 648 1416 861 359 159 2042 Fragments arranged by size: 2042 1416 861 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2474 2509 2709 2901 3578 4726 4971 1606 Size: 868 35 200 192 677 1148 245 2120 Fragments arranged by size: 2120 1148 868 677 245 200 192 35 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3994 Fragments arranged by size: 3994 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5485 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5485 NotI GC'GGCC_GC Cuts at: 2932 2932 Size: 5485 NspI r_CATG'y Cuts at: 1132 1229 2485 2843 4388 1132 Size: 97 1256 358 1545 2229 Fragments arranged by size: 2229 1545 1256 358 97 PasI CC'CwG_GG Cuts at: 1348 2715 1348 Size: 1367 4118 PciI A'CATG_T Cuts at: 4384 4384 Size: 5485 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5485 PfoI T'CCnGG_A Cuts at: 2745 2745 Size: 5485 PmeI GTTT'AAAC Cuts at: 2789 2789 Size: 5485 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3554 3586 5067 5099 3554 Size: 32 1481 32 3940 Fragments arranged by size: 3940 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2043 2043 Size: 5485 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5485 PsiI TTA'TAA Cuts at: 3055 3055 Size: 5485 PspOMI G'GGCC_C Cuts at: 2625 2625 Size: 5485 PstI C_TGCA'G Cuts at: 830 2692 2837 830 Size: 1862 145 3478 Fragments arranged by size: 3478 1862 145 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5485 PvuII CAG'CTG Cuts at: 2474 2509 2709 2474 Size: 35 200 5250 Fragments arranged by size: 5250 200 35 SalI G'TCGA_C Cuts at: 1936 2827 1936 Size: 891 4594 SanDI GG'GwC_CC Cuts at: 2043 2043 Size: 5485 SbfI CC_TGCA'GG Cuts at: 2837 2837 Size: 5485 ScaI AGT'ACT Cuts at: 3733 5445 3733 Size: 1712 3773 SfcI C'TryA_G Cuts at: 826 1045 2688 2833 3968 4649 4840 826 Size: 219 1643 145 1135 681 191 1471 Fragments arranged by size: 1643 1471 1135 681 219 191 145 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5485 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5485 SmaI CCC'GGG Cuts at: 1872 2812 1872 Size: 940 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3589 4490 4752 820 Size: 197 926 1646 901 262 1553 Fragments arranged by size: 1646 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3276 493 Size: 2783 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5485 SphI G_CATG'C Cuts at: 1132 2843 1132 Size: 1711 3774 SspI AAT'ATT Cuts at: 5 52 3409 5 Size: 47 3357 2081 Fragments arranged by size: 3357 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5485 StyI C'CwwG_G Cuts at: 513 1065 2004 2924 513 Size: 552 939 920 3074 Fragments arranged by size: 3074 939 920 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2385 2447 3512 3529 3682 3867 4312 Size: 1255 805 62 1065 17 153 185 445 Cuts at: 4312 325 Size: 1498 Fragments arranged by size: 1498 1255 1065 805 445 185 153 62 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3731 5443 96 Size: 274 80 33 51 1443 1754 1712 138 Fragments arranged by size: 1754 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2336 2368 5445 5477 2336 Size: 32 3077 32 2344 Fragments arranged by size: 3077 2344 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5485 XbaI T'CTAG_A Cuts at: 2821 2821 Size: 5485 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2022 2022 Size: 5485 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5485 XmaI C'CCGG_G Cuts at: 1870 2810 1870 Size: 940 4545 XmnI GAAnn'nnTTC Cuts at: 3614 3614 Size: 5485 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3293 276 Size: 53 83 186 936 1759 2468 Fragments arranged by size: 2468 1759 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI