################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BamHI BbrPI BbvCI BciVI BclI BfaI BfrI BlnI BsaAI BsaBI BsaI BsaWI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 1131 GACGTC GACGT^C AfaI 120 GTAC GT^AC Alw44I 1075 GTGCAC G^TGCAC AlwI 1085 GGATC GGATC(4/5) ApaLI 1075 GTGCAC G^TGCAC AspI 391 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 230 TGGCCA TGG^CCA BbeI 300 GGCGCC GGCGC^C BcgI 156 CGAGGAGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 881 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1221 AGATCT A^GATCT BlpI 279 GCTGAGC GC^T[ACGT]AGC Bpu1102I 279 GCTGAGC GC^T[ACGT]AGC BsgI 490 GTGCAG GTGCAG(16/14) BsiEI 638 CGACCG CG[GA][CT]^CG BspMI 98 ACCTGC ACCTGC(4/8) BstEII 472 GGTCACC G^GT[ACGT]ACC BstPI 472 GGTCACC G^GT[ACGT]ACC BstYI 1221 AGATCT [GA]^GATC[CT] CelII 279 GCTGAGC GC^T[ACGT]AGC DsaI 713 CCGTGG C^C[GA][CT]GG EcoO65I 472 GGTCACC G^GT[ACGT]ACC HinfI 1024 GACTC G^A[ACGT]TC KasI 300 GGCGCC G^GCGCC MflI 1221 AGATCT [GA]^GATC[CT] MluNI 230 TGGCCA TGG^CCA MscI 230 TGGCCA TGG^CCA NarI 300 GGCGCC GG^CGCC PflFI 391 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1024 GACTC GAGTC(4/5) RsaI 120 GTAC GT^AC SacI 688 GAGCTC GAGCT^C SbfI 99 CCTGCAGG CCTGCA^GG ScaI 119 AGTACT AGT^ACT SfoI 300 GGCGCC GGC^GCC Sse8387I 99 CCTGCAGG CCTGCA^GG Tth111I 391 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 734 CCAACTTCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 1221 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AvaI 196 CCCGAG C^[CT]CG[GA]G 256 CCCGAG AviII 239 TGCGCA TGC^GCA 462 TGCGCA BanI 300 GGCGCC G^G[CT][GA]CC 761 GGCACC BbsI 865 GAAGAC GAAGAC(2/6) 978 GAAGAC BpuAI 865 GAAGAC GAAGAC(2/6) 978 GAAGAC BseRI 822 CTCCTC GAGGAG(10/8) 157 GAGGAG BsmAI 359 GTCTC GTCTC(1/5) 1228 GTCTC BsoBI 196 CCCGAG C^[CT]CG[GA]G 256 CCCGAG BsrFI 289 ACCGGC [GA]^CCGG[CT] 1058 ACCGGC BstXI 623 CCATCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1254 CCAGCAACCTGG Cfr10I 289 ACCGGC [GA]^CCGG[CT] 1058 ACCGGC DpnII 1086 GATC ^GATC 1222 GATC FspI 239 TGCGCA TGC^GCA 462 TGCGCA HaeII 300 GGCGCC [GA]GCGC^[CT] 446 AGCGCC HgaI 217 GCGTC GACGC(5/10) 680 GCGTC MboI 1086 GATC ^GATC 1222 GATC MspA1I 424 CCGCTG C[AC]G^C[GT]G 1080 CAGCGG NdeII 1086 GATC ^GATC 1222 GATC NlaIII 399 CATG CATG^ 948 CATG PpuMI 729 AGGACCC [GA]G^G[AT]CC[CT] 1208 AGGACCC PstI 100 CTGCAG CTGCA^G 754 CTGCAG Sau3AI 1086 GATC ^GATC 1222 GATC SexAI 88 ACCTGGT A^CC[AT]GGT 392 ACCTGGT SfcI 100 CTGCAG C^T[GA][CT]AG 754 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AcyI AluI AspHI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrBI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HpaII HphI ItaI MaeII MaeIII MboII MnlI MslI MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI SfaNI TaqI TseI Tsp45I TspRI TthHB8I