(Circular) (Six-base) MAPSORT of: pFN21AE1374.seq Check: 9895 from: 1 to: 5989 pFN21AE1374 5989 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3140 3799 278 Size: 53 83 186 936 1604 659 2468 Fragments arranged by size: 2468 1604 936 659 186 83 53 AccI GT'mk_AC Cuts at: 1937 3332 1937 Size: 1395 4594 Acc65I G'GTAC_C Cuts at: 3310 3310 Size: 5989 AclI AA'CG_TT Cuts at: 1563 4116 4489 1563 Size: 2553 373 3063 Fragments arranged by size: 3063 2553 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4052 5436 5704 1632 Size: 2420 1384 268 1917 Fragments arranged by size: 2420 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5792 AflIII A'CryG_T Cuts at: 1203 4888 5865 1203 Size: 3685 977 1327 Fragments arranged by size: 3685 1327 977 AgeI A'CCGG_T Cuts at: 4820 4820 Size: 5989 AhdI GACnn_n'nnGTC Cuts at: 4718 4718 Size: 5989 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5989 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5989 AlwNI CAG_nnn'CTG Cuts at: 3732 5304 3732 Size: 1572 4417 ApaLI G'TGCA_C Cuts at: 3080 4046 5202 3080 Size: 966 1156 3867 Fragments arranged by size: 3867 1156 966 ApoI r'AATT_y Cuts at: 1493 3298 3524 3676 5785 1493 Size: 1805 226 152 2109 1697 Fragments arranged by size: 2109 1805 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4543 160 Size: 872 3511 1606 Fragments arranged by size: 3511 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 AvaI C'yCGr_G Cuts at: 1870 1943 2201 2261 3314 1870 Size: 73 258 60 1053 4545 Fragments arranged by size: 4545 1053 258 73 60 BamHI G'GATC_C Cuts at: 3319 3686 3319 Size: 367 5622 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2305 2766 3310 4765 618 Size: 325 217 627 518 461 544 1455 1842 Fragments arranged by size: 1842 1455 627 544 518 461 325 217 BanII G_rGCy'C Cuts at: 1391 2606 2697 2861 2888 3104 3308 1391 Size: 1215 91 164 27 216 204 4072 Fragments arranged by size: 4072 1215 216 204 164 91 27 BbeI G_GCGC'C Cuts at: 2309 2309 Size: 5989 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2877 2990 928 Size: 971 978 113 3927 Fragments arranged by size: 3927 978 971 113 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2150 2184 4180 4214 1903 Size: 34 213 34 1996 34 3678 Fragments arranged by size: 3678 1996 213 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3881 5091 3881 Size: 1210 4779 BclI T'GATC_A Cuts at: 1548 1548 Size: 5989 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2892 4600 136 Size: 107 122 71 631 605 1220 1708 1525 Fragments arranged by size: 1708 1525 1220 631 605 122 107 71 BglII A'GATC_T Cuts at: 3226 5984 3226 Size: 2758 3231 BlpI GC'TnA_GC Cuts at: 2285 3406 2285 Size: 1121 4868 Bme1580I G_kGCm'C Cuts at: 1790 2769 3084 4050 5206 1790 Size: 979 315 966 1156 2573 Fragments arranged by size: 2573 1156 979 966 315 BmgBI CAC'GTC Cuts at: 1318 2074 2875 1318 Size: 756 801 4432 Fragments arranged by size: 4432 801 756 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3720 4678 442 Size: 416 53 83 399 24 2303 958 1753 Fragments arranged by size: 2303 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5989 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 2815 3028 4649 1239 Size: 1214 362 213 1621 2579 Fragments arranged by size: 2579 1621 1214 362 213 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4114 4979 5277 4114 Size: 865 298 4826 Fragments arranged by size: 4826 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4652 882 Size: 617 3153 2219 Fragments arranged by size: 3153 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3780 493 Size: 711 2576 2702 Fragments arranged by size: 2702 2576 711 BsaBI GATnn'nnATC Cuts at: 5983 5983 Size: 5989 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2222 2306 3137 3796 Size: 53 83 186 936 689 84 831 659 Cuts at: 3796 4178 4813 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 831 689 659 635 382 186 84 83 53 BsaWI w'CCGG_w Cuts at: 4422 4820 5094 5241 5652 4422 Size: 398 274 147 411 4759 Fragments arranged by size: 4759 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3098 3128 1178 Size: 30 165 30 1695 30 4039 Fragments arranged by size: 4039 1695 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 2818 1186 Size: 991 641 4357 Fragments arranged by size: 4357 991 641 BseYI C'CCAG_C Cuts at: 2188 2788 2819 2912 3054 3250 5192 5688 2188 Size: 600 31 93 142 196 1942 496 2489 Fragments arranged by size: 2489 1942 600 496 196 142 93 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2516 1163 Size: 573 780 4636 Fragments arranged by size: 4636 780 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2646 3439 4200 5228 1540 Size: 275 186 645 793 761 1028 2301 Fragments arranged by size: 2301 1028 793 761 645 275 186 BsiHKAI G_wGCw'C Cuts at: 2322 2441 2697 3084 4050 4135 5206 2322 Size: 119 256 387 966 85 1071 3105 Fragments arranged by size: 3105 1071 966 387 256 119 85 BsmI GAATG_Cn' Cuts at: 3500 3593 3500 Size: 93 5896 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2322 2441 2606 2697 2769 2861 2888 Size: 399 532 119 165 91 72 92 27 Cuts at: 2888 3084 3104 3308 4050 4135 5206 1391 Size: 196 20 204 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 532 399 204 196 165 119 92 91 85 72 27 20 BspEI T'CCGG_A Cuts at: 5652 5652 Size: 5989 BspHI T'CATG_A Cuts at: 3878 5608 3878 Size: 1730 4259 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2112 3344 844 Size: 1268 1232 3489 Fragments arranged by size: 3489 1268 1232 BsrBI CCG'CTC Cuts at: 1394 1823 2020 3119 3220 3876 1394 Size: 429 197 1099 101 656 3507 Fragments arranged by size: 3507 1099 656 429 197 101 BsrDI GCAATG_nn' Cuts at: 65 1248 4484 4658 65 Size: 1183 3236 174 1396 Fragments arranged by size: 3236 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2294 3063 4633 4820 1700 Size: 110 484 769 1570 187 2869 Fragments arranged by size: 2869 1570 769 484 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5989 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5989 BssSI C'ACGA_G Cuts at: 2138 2881 2923 4049 5061 2138 Size: 743 42 1126 1012 3066 Fragments arranged by size: 3066 1126 1012 743 42 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5989 BstBI TT'CG_AA Cuts at: 4793 4793 Size: 5989 BstEII G'GTnAC_C Cuts at: 1184 2477 1184 Size: 1293 4696 BstXI CCAn_nnnn'nTGG Cuts at: 2635 3266 2635 Size: 631 5358 BstYI r'GATC_y Cuts at: 1972 3226 3319 3686 4071 4088 5540 5626 5638 Size: 1254 93 367 385 17 1452 86 12 Cuts at: 5638 5984 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1254 385 367 346 93 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2718 513 Size: 552 1653 3784 Fragments arranged by size: 3784 1653 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3058 487 Size: 683 56 1832 3418 Fragments arranged by size: 3418 1832 683 56 BtsI GCAGTG_nn' Cuts at: 2477 3505 4299 4319 2477 Size: 1028 794 20 4147 Fragments arranged by size: 4147 1028 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5989 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5954 DraI TTT'AAA Cuts at: 3293 3293 Size: 5989 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4996 809 Size: 507 3680 1802 Fragments arranged by size: 3680 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2235 2517 3180 Size: 54 1425 50 275 18 405 282 663 Cuts at: 3180 3436 4325 5954 8 Size: 256 889 1629 43 Fragments arranged by size: 1629 1425 889 663 405 282 275 256 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3436 1537 Size: 275 1624 4090 Fragments arranged by size: 4090 1624 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3919 1353 Size: 2566 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2390 4576 4950 5096 387 Size: 1064 138 801 2186 374 146 1280 Fragments arranged by size: 2186 1280 1064 801 374 146 138 EcoICRI GAG'CTC Cuts at: 2695 2695 Size: 5989 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2453 2815 3028 4052 4649 5436 5704 Size: 393 821 362 213 1024 597 787 268 Cuts at: 5704 1239 Size: 1524 Fragments arranged by size: 1524 1024 821 787 597 393 362 268 213 EcoNI CCTnn'n_nnAGG Cuts at: 2030 2417 2459 2030 Size: 387 42 5560 Fragments arranged by size: 5560 387 42 EcoO109I rG'GnC_Cy Cuts at: 2059 2735 3214 2059 Size: 676 479 4834 Fragments arranged by size: 4834 676 479 EcoRI G'AATT_C Cuts at: 3298 3676 3298 Size: 378 5611 FspI TGC'GCA Cuts at: 2246 2469 4495 2246 Size: 223 2026 3740 Fragments arranged by size: 3740 2026 223 HaeII r_GCGC'y Cuts at: 1678 2309 2455 5136 1678 Size: 631 146 2681 2531 Fragments arranged by size: 2681 2531 631 146 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3218 3250 4630 4662 4704 Size: 32 135 32 1646 32 1380 32 42 Cuts at: 4704 4736 1373 Size: 32 2626 Fragments arranged by size: 2626 1646 1380 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3333 3579 669 Size: 988 281 1395 246 3079 Fragments arranged by size: 3079 1395 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5989 HpaI GTT'AAC Cuts at: 3579 3579 Size: 5989 KasI G'GCGC_C Cuts at: 2305 2305 Size: 5989 KpnI G_GTAC'C Cuts at: 3314 3314 Size: 5989 MfeI C'AATT_G Cuts at: 3588 3588 Size: 5989 MluI A'CGCG_T Cuts at: 5865 5865 Size: 5989 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2349 5103 5287 1305 Size: 1044 2754 184 2007 Fragments arranged by size: 2754 2007 1044 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2237 10 Size: 54 1425 343 405 3762 Fragments arranged by size: 3762 1425 405 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 2702 3077 3947 4306 4465 518 Size: 648 1347 189 375 870 359 159 2042 Fragments arranged by size: 2042 1347 870 648 375 359 189 159 MspA1I CmG'CkG Cuts at: 1606 2431 3087 3405 4082 5230 5475 1606 Size: 825 656 318 677 1148 245 2120 Fragments arranged by size: 2120 1148 825 677 656 318 245 NarI GG'CG_CC Cuts at: 2306 2306 Size: 5989 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5437 NdeI CA'TA_TG Cuts at: 387 387 Size: 5989 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5989 NotI GC'GGCC_GC Cuts at: 3436 3436 Size: 5989 NspI r_CATG'y Cuts at: 1132 1229 3347 4892 1132 Size: 97 2118 1545 2229 Fragments arranged by size: 2229 2118 1545 97 PasI CC'CwG_GG Cuts at: 1348 2749 1348 Size: 1401 4588 PciI A'CATG_T Cuts at: 4888 4888 Size: 5989 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5989 PfoI T'CCnGG_A Cuts at: 2652 2708 2652 Size: 56 5933 PmeI GTTT'AAAC Cuts at: 3293 3293 Size: 5989 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4058 4090 5571 5603 4058 Size: 32 1481 32 4444 Fragments arranged by size: 4444 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2735 3214 2735 Size: 479 5510 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5989 PsiI TTA'TAA Cuts at: 3559 3559 Size: 5989 PstI C_TGCA'G Cuts at: 830 2109 2763 3341 830 Size: 1279 654 578 3478 Fragments arranged by size: 3478 1279 654 578 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5989 SacI G_AGCT'C Cuts at: 2697 2697 Size: 5989 SalI G'TCGA_C Cuts at: 1936 3331 1936 Size: 1395 4594 SbfI CC_TGCA'GG Cuts at: 2109 3341 2109 Size: 1232 4757 ScaI AGT'ACT Cuts at: 2126 4237 5949 2126 Size: 2111 1712 2166 Fragments arranged by size: 2166 2111 1712 SexAI A'CCwGG_T Cuts at: 2093 2397 2093 Size: 304 5685 SfcI C'TryA_G Cuts at: 826 1045 2105 2759 3337 4472 5153 5344 826 Size: 219 1060 654 578 1135 681 191 1471 Fragments arranged by size: 1471 1135 1060 681 654 578 219 191 SfoI GGC'GCC Cuts at: 2307 2307 Size: 5989 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5989 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5989 SmaI CCC'GGG Cuts at: 1872 3316 1872 Size: 1444 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4093 4994 5256 820 Size: 197 926 2150 901 262 1553 Fragments arranged by size: 2150 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3780 493 Size: 3287 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5989 SphI G_CATG'C Cuts at: 1132 3347 1132 Size: 2215 3774 SspI AAT'ATT Cuts at: 5 52 3913 5 Size: 47 3861 2081 Fragments arranged by size: 3861 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5989 StyI C'CwwG_G Cuts at: 513 1065 3428 513 Size: 552 2363 3074 Fragments arranged by size: 3074 2363 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2045 2660 3068 4016 4033 4186 4371 Size: 1255 465 615 408 948 17 153 185 Cuts at: 4371 4816 325 Size: 445 1498 Fragments arranged by size: 1498 1255 948 615 465 445 408 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 4235 5947 Size: 274 80 33 51 1443 147 2111 1712 Cuts at: 5947 96 Size: 138 Fragments arranged by size: 2111 1712 1443 274 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2369 2401 3098 3130 5949 5981 2369 Size: 32 697 32 2819 32 2377 Fragments arranged by size: 2819 2377 697 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2399 1316 Size: 1083 4906 XbaI T'CTAG_A Cuts at: 3325 3325 Size: 5989 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2746 2746 Size: 5989 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5989 XmaI C'CCGG_G Cuts at: 1870 3314 1870 Size: 1444 4545 XmnI GAAnn'nnTTC Cuts at: 4118 4118 Size: 5989 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3138 3797 276 Size: 53 83 186 936 1604 659 2468 Fragments arranged by size: 2468 1604 936 659 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI