(Linear) (Six-base) MAPSORT of: KIEE1374.seq Check: 3147 from: 1 to: 1284 KIEE1374.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AatII G_ACGT'C Cuts at: 0 1135 1284 Size: 1135 149 ApaLI G'TGCA_C Cuts at: 0 1075 1284 Size: 1075 209 AvaI C'yCGr_G Cuts at: 0 196 256 1284 Size: 196 60 1028 Fragments arranged by size: 1028 196 60 BanI G'GyrC_C Cuts at: 0 300 761 1284 Size: 300 461 523 Fragments arranged by size: 523 461 300 BanII G_rGCy'C Cuts at: 0 601 692 856 883 1099 1284 Size: 601 91 164 27 216 185 Fragments arranged by size: 601 216 185 164 91 27 BbeI G_GCGC'C Cuts at: 0 304 1284 Size: 304 980 BbsI GAAGACnn'nnnn_ Cuts at: 0 872 985 1284 Size: 872 113 299 Fragments arranged by size: 872 299 113 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 145 179 1284 Size: 145 34 1105 Fragments arranged by size: 1105 145 34 BglI GCCn_nnn'nGGC Cuts at: 0 887 1284 Size: 887 397 BglII A'GATC_T Cuts at: 0 1221 1284 Size: 1221 63 BlpI GC'TnA_GC Cuts at: 0 280 1284 Size: 280 1004 Bme1580I G_kGCm'C Cuts at: 0 764 1079 1284 Size: 764 315 205 Fragments arranged by size: 764 315 205 BmgBI CAC'GTC Cuts at: 0 69 870 1284 Size: 69 801 414 Fragments arranged by size: 801 414 69 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 448 810 1023 1284 Size: 448 362 213 261 Fragments arranged by size: 448 362 261 213 BsaHI Gr'CG_yC Cuts at: 0 217 301 1132 1284 Size: 217 84 831 152 Fragments arranged by size: 831 217 152 84 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1093 1123 1284 Size: 1093 30 161 Fragments arranged by size: 1093 161 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 172 813 1284 Size: 172 641 471 Fragments arranged by size: 641 471 172 BseYI C'CCAG_C Cuts at: 0 183 783 814 907 1049 1245 1284 Size: 183 600 31 93 142 196 39 Fragments arranged by size: 600 196 183 142 93 39 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 511 1284 Size: 511 773 BsiEI CG_ry'CG Cuts at: 0 641 1284 Size: 641 643 BsiHKAI G_wGCw'C Cuts at: 0 317 436 692 1079 1284 Size: 317 119 256 387 205 Fragments arranged by size: 387 317 256 205 119 Bsp1286I G_dGCh'C Cuts at: 0 317 436 601 692 764 856 883 1079 Size: 317 119 165 91 72 92 27 196 Cuts at: 1079 1099 1284 Size: 20 185 Fragments arranged by size: 317 196 185 165 119 92 91 72 27 20 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 107 1284 Size: 107 1177 BsrBI CCG'CTC Cuts at: 0 15 1114 1215 1284 Size: 15 1099 101 69 Fragments arranged by size: 1099 101 69 15 BsrFI r'CCGG_y Cuts at: 0 289 1058 1284 Size: 289 769 226 Fragments arranged by size: 769 289 226 BssSI C'ACGA_G Cuts at: 0 133 876 918 1284 Size: 133 743 42 366 Fragments arranged by size: 743 366 133 42 BstEII G'GTnAC_C Cuts at: 0 472 1284 Size: 472 812 BstXI CCAn_nnnn'nTGG Cuts at: 0 630 1261 1284 Size: 630 631 23 Fragments arranged by size: 631 630 23 BstYI r'GATC_y Cuts at: 0 1221 1284 Size: 1221 63 BtgI C'CryG_G Cuts at: 0 713 1284 Size: 713 571 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1053 1284 Size: 1053 231 BtsI GCAGTG_nn' Cuts at: 0 472 1284 Size: 472 812 EaeI y'GGCC_r Cuts at: 0 230 512 1175 1284 Size: 230 282 663 109 Fragments arranged by size: 663 282 230 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 385 1284 Size: 385 899 EcoICRI GAG'CTC Cuts at: 0 690 1284 Size: 690 594 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 448 810 1023 1284 Size: 448 362 213 261 Fragments arranged by size: 448 362 261 213 EcoNI CCTnn'n_nnAGG Cuts at: 0 25 412 454 1284 Size: 25 387 42 830 Fragments arranged by size: 830 387 42 25 EcoO109I rG'GnC_Cy Cuts at: 0 54 730 1209 1284 Size: 54 676 479 75 Fragments arranged by size: 676 479 75 54 FspI TGC'GCA Cuts at: 0 241 464 1284 Size: 241 223 820 Fragments arranged by size: 820 241 223 HaeII r_GCGC'y Cuts at: 0 304 450 1284 Size: 304 146 834 Fragments arranged by size: 834 304 146 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1213 1245 1284 Size: 1213 32 39 Fragments arranged by size: 1213 39 32 KasI G'GCGC_C Cuts at: 0 300 1284 Size: 300 984 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 344 1284 Size: 344 940 MscI TGG'CCA Cuts at: 0 232 1284 Size: 232 1052 MslI CAynn'nnrTG Cuts at: 0 508 697 1072 1284 Size: 508 189 375 212 Fragments arranged by size: 508 375 212 189 MspA1I CmG'CkG Cuts at: 0 426 1082 1284 Size: 426 656 202 Fragments arranged by size: 656 426 202 NarI GG'CG_CC Cuts at: 0 301 1284 Size: 301 983 PasI CC'CwG_GG Cuts at: 0 744 1284 Size: 744 540 PfoI T'CCnGG_A Cuts at: 0 647 703 1284 Size: 647 56 581 Fragments arranged by size: 647 581 56 PpuMI rG'GwC_Cy Cuts at: 0 730 1209 1284 Size: 730 479 75 Fragments arranged by size: 730 479 75 PstI C_TGCA'G Cuts at: 0 104 758 1284 Size: 104 654 526 Fragments arranged by size: 654 526 104 SacI G_AGCT'C Cuts at: 0 692 1284 Size: 692 592 SbfI CC_TGCA'GG Cuts at: 0 104 1284 Size: 104 1180 ScaI AGT'ACT Cuts at: 0 121 1284 Size: 121 1163 SexAI A'CCwGG_T Cuts at: 0 88 392 1284 Size: 88 304 892 Fragments arranged by size: 892 304 88 SfcI C'TryA_G Cuts at: 0 100 754 1284 Size: 100 654 530 Fragments arranged by size: 654 530 100 SfoI GGC'GCC Cuts at: 0 302 1284 Size: 302 982 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 40 655 1063 1284 Size: 40 615 408 221 Fragments arranged by size: 615 408 221 40 TatI w'GTAC_w Cuts at: 0 119 1284 Size: 119 1165 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 364 396 1093 1125 1284 Size: 364 32 697 32 159 Fragments arranged by size: 697 364 159 32 32 Tth111I GACn'n_nGTC Cuts at: 0 394 1284 Size: 394 890 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 741 1284 Size: 741 543 ZraI GAC'GTC Cuts at: 0 1133 1284 Size: 1133 151 Enzymes that do cut: AatII ApaLI AvaI BanI BanII BbeI BbsI BcgI BglI BglII BlpI Bme1580I BmgBI BpmI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstEII BstXI BstYI BtgI BtgZI BtsI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MmeI MscI MslI MspA1I NarI PasI PfoI PpuMI PstI SacI SbfI ScaI SexAI SfcI SfoI TaqII TatI TstI Tth111I XcmI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EarI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI XbaI XhoI XmaI XmnI