################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcyI AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII BalI BanI BbeI BbrPI BcgI BciVI BclI BglI BsaAI BsaBI BsaHI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CfoI Cfr10I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AccIII 494 TCCGGA T^CCGGA AcsI 760 GAATTC [GA]^AATT[CT] AflII 868 CTTAAG C^TTAAG AflIII 160 ACATGT A^C[GA][CT]GT AocI 268 CCTCAGG CC^T[ACGT]AGG ApaI 274 GGGCCC GGGCC^C ApoI 760 GAATTC [GA]^AATT[CT] Asp700I 429 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 993 CCTAGG C^CTAGG BamHI 938 GGATCC G^GATCC BanII 274 GGGCCC G[GA]GC[CT]^C BbsI 18 GAAGAC GAAGAC(2/6) BbvCI 536 CCTCAGC CCTCAGC(-5/-2) BcnI 913 CCGGG CC^[GC]GG BfrI 868 CTTAAG C^TTAAG BglII 219 AGATCT A^GATCT BlnI 993 CCTAGG C^CTAGG BlpI 571 GCTCAGC GC^T[ACGT]AGC BmyI 274 GGGCCC G[AGT]GC[ACT]^C BpmI 586 CTCCAG CTGGAG(16/14) Bpu1102I 571 GCTCAGC GC^T[ACGT]AGC BpuAI 18 GAAGAC GAAGAC(2/6) BsaI 998 GAGACC GGTCTC(1/5) BseAI 494 TCCGGA T^CCGGA BseRI 454 GAGGAG GAGGAG(10/8) Bsp1286I 274 GGGCCC G[AGT]GC[ACT]^C BspEI 494 TCCGGA T^CCGGA BspHI 1062 TCATGA T^CATGA BspLU11I 160 ACATGT A^CATGT BspMI 356 ACCTGC ACCTGC(4/8) BsrI 855 ACTGG ACTGG(1/-1) BstAPI 231 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 348 GGTGACC G^GT[ACGT]ACC BstPI 348 GGTGACC G^GT[ACGT]ACC BstXI 44 CCATCTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 268 CCTCAGG CC^T[ACGT]AGG CelII 571 GCTCAGC GC^T[ACGT]AGC DraI 130 TTTAAA TTT^AAA DrdI 100 GACGGTGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 618 CCACGG C^C[GA][CT]GG EaeI 177 TGGCCG [CT]^GGCC[GA] EarI 429 GAAGAG CTCTTC(1/4) Eco81I 268 CCTCAGG CC^T[ACGT]AGG EcoO65I 348 GGTGACC G^GT[ACGT]ACC EcoRI 760 GAATTC G^AATTC HphI 348 GGTGA GGTGA(8/7) Ksp632I 429 GAAGAG CTCTTC(1/4) MaeII 208 ACGT A^CGT MroI 494 TCCGGA T^CCGGA NciI 913 CCGGG CC^[GC]GG NspI 160 ACATGT [GA]CATG^[CT] PleI 962 GAGTC GAGTC(4/5) PpuMI 384 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 1062 TCATGA T^CATGA ScaI 125 AGTACT AGT^ACT SmlI 868 CTTAAG C^T[CT][GA]AG StuI 590 AGGCCT AGG^CCT TaqI 138 TCGA T^CGA TfiI 256 GAATC G^A[AT]TC TthHB8I 138 TCGA T^CGA XmnI 429 GAAGAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BsaWI 494 TCCGGA [AT]^CCGG[AT] 516 ACCGGA BstYI 219 AGATCT [GA]^GATC[CT] 938 GGATCC HinfI 256 GAATC G^A[ACGT]TC 962 GAGTC MflI 219 AGATCT [GA]^GATC[CT] 938 GGATCC MspA1I 251 CAGCTG C[AC]G^C[GT]G 831 CAGCTG MwoI 231 GCAGACCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 297 GCGACTACTGC PstI 340 CTGCAG CTGCA^G 376 CTGCAG PvuII 251 CAGCTG CAG^CTG 831 CAGCTG SfaNI 394 GATGC GCATC(5/9) 989 GCATC Tsp45I 349 GTGAC ^GT[GC]AC 370 GTGAC TspRI 374 CACTG CA[GC]TG[ACGT][ACGT]^ 854 CACTG XhoII 219 AGATCT [GA]^GATC[CT] 938 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 53 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BbvI BfaI BsaJI BsgI BsiYI BslI BsmAI BsmFI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HpaII ItaI MaeI MaeIII MboI MboII MnlI MseI MspI MvaI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI StyI Tru9I TseI Tsp509I