(Circular) (Six-base) MAPSORT of: pFN21AB6526.seq Check: 5277 from: 1 to: 5782 pFN21AB6526 5782 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2415 3845 5229 5497 1632 Size: 783 1430 1384 268 1917 Fragments arranged by size: 1917 1430 1384 783 268 AflII C'TTAA_G Cuts at: 820 1017 2873 820 Size: 197 1856 3729 Fragments arranged by size: 3729 1856 197 AflIII A'CryG_T Cuts at: 1203 2165 4681 5658 1203 Size: 962 2516 977 1327 Fragments arranged by size: 2516 1327 977 962 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AlwNI CAG_nnn'CTG Cuts at: 2242 2393 2534 3525 5097 2242 Size: 151 141 991 1572 2927 Fragments arranged by size: 2927 1572 991 151 141 ApaI G_GGCC'C Cuts at: 2283 2283 Size: 5782 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 2765 3091 3317 3469 5578 1493 Size: 1272 326 226 152 2109 1697 Fragments arranged by size: 2109 1697 1272 326 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 3107 1870 Size: 73 1164 4545 Fragments arranged by size: 4545 1164 73 AvrII C'CTAG_G Cuts at: 2998 2998 Size: 5782 BamHI G'GATC_C Cuts at: 2943 3112 3479 2943 Size: 169 367 5246 Fragments arranged by size: 5246 367 169 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3103 4558 618 Size: 325 217 627 1316 1455 1842 Fragments arranged by size: 1842 1455 1316 627 325 217 BanII G_rGCy'C Cuts at: 1391 2283 3101 1391 Size: 892 818 4072 Fragments arranged by size: 4072 892 818 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2030 928 Size: 971 131 4680 Fragments arranged by size: 4680 971 131 BbvCI CC'TCA_GC Cuts at: 2542 2542 Size: 5782 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4393 136 Size: 107 122 71 631 605 2721 1525 Fragments arranged by size: 2721 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2224 5777 2224 Size: 3553 2229 BlpI GC'TnA_GC Cuts at: 2577 3199 2577 Size: 622 5160 Bme1580I G_kGCm'C Cuts at: 1790 2283 3843 4999 1790 Size: 493 1560 1156 2573 Fragments arranged by size: 2573 1560 1156 493 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3513 4471 442 Size: 416 53 83 399 24 2096 958 1753 Fragments arranged by size: 2096 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2485 2485 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2576 4442 1239 Size: 1337 1866 2579 Fragments arranged by size: 2579 1866 1337 Bpu10I CC'TnA_GC Cuts at: 2542 2542 Size: 5782 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3907 4772 5070 3907 Size: 865 298 4619 Fragments arranged by size: 4619 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2997 4445 882 Size: 617 1498 1448 2219 Fragments arranged by size: 2219 1498 1448 617 BsaAI yAC'GTr Cuts at: 493 1204 3573 493 Size: 711 2369 2702 Fragments arranged by size: 2702 2369 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3589 3971 4606 275 Size: 53 83 186 936 2056 382 635 1451 Fragments arranged by size: 2056 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2499 2521 4215 4613 4887 5034 5445 2499 Size: 22 1694 398 274 147 411 2836 Fragments arranged by size: 2836 1694 411 398 274 147 22 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5557 Fragments arranged by size: 5557 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2474 1186 Size: 1288 4494 BseYI C'CCAG_C Cuts at: 2254 2938 4985 5481 2254 Size: 684 2047 496 2555 Fragments arranged by size: 2555 2047 684 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2255 2294 2647 1163 Size: 573 519 39 353 4298 Fragments arranged by size: 4298 573 519 353 39 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3232 3993 5021 1540 Size: 275 186 1231 761 1028 2301 Fragments arranged by size: 2301 1231 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3843 3928 4999 3843 Size: 85 1071 4626 Fragments arranged by size: 4626 1071 85 BsmI GAATG_Cn' Cuts at: 3293 3386 3293 Size: 93 5689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2283 3101 3843 3928 4999 1391 Size: 399 493 818 742 85 1071 2174 Fragments arranged by size: 2174 1071 818 742 493 399 85 BspEI T'CCGG_A Cuts at: 2499 5445 2499 Size: 2946 2836 BspHI T'CATG_A Cuts at: 3067 3671 5401 3067 Size: 604 1730 3448 Fragments arranged by size: 3448 1730 604 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2370 3137 844 Size: 1526 767 3489 Fragments arranged by size: 3489 1526 767 BsrBI CCG'CTC Cuts at: 1394 1823 3669 1394 Size: 429 1846 3507 Fragments arranged by size: 3507 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4426 4613 1700 Size: 110 2616 187 2869 Fragments arranged by size: 2869 2616 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 2242 794 Size: 1448 4334 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 2353 1184 Size: 1169 4613 BstXI CCAn_nnnn'nTGG Cuts at: 2056 2056 Size: 5782 BstYI r'GATC_y Cuts at: 1972 2224 2943 3112 3479 3864 3881 5333 5419 Size: 252 719 169 367 385 17 1452 86 Cuts at: 5419 5431 5777 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 719 385 367 346 252 169 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2274 2274 Size: 5782 BtgI C'CryG_G Cuts at: 513 1065 2623 513 Size: 552 1558 3672 Fragments arranged by size: 3672 1558 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3033 487 Size: 683 56 1807 3236 Fragments arranged by size: 3236 1807 683 56 BtsI GCAGTG_nn' Cuts at: 2378 3298 4092 4112 2378 Size: 920 794 20 4048 Fragments arranged by size: 4048 920 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5747 DraI TTT'AAA Cuts at: 2137 3086 2137 Size: 949 4833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2111 4789 809 Size: 507 795 2678 1802 Fragments arranged by size: 2678 1802 795 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2182 3229 4118 Size: 54 1425 50 275 18 352 1047 889 Cuts at: 4118 5747 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1047 889 352 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3229 1537 Size: 275 1417 4090 Fragments arranged by size: 4090 1417 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2429 3712 1353 Size: 1076 1283 3423 Fragments arranged by size: 3423 1283 1076 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2546 4369 4743 4889 387 Size: 1064 138 957 1823 374 146 1280 Fragments arranged by size: 1823 1280 1064 957 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2415 2576 3845 4442 5229 5497 1239 Size: 393 783 161 1269 597 787 268 1524 Fragments arranged by size: 1524 1269 787 783 597 393 268 161 EcoO109I rG'GnC_Cy Cuts at: 2279 2280 2390 2531 2851 2279 Size: 1 110 141 320 5210 Fragments arranged by size: 5210 320 141 110 1 EcoRI G'AATT_C Cuts at: 2765 3091 3469 2765 Size: 326 378 5078 Fragments arranged by size: 5078 378 326 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 4929 1678 Size: 3251 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2619 2651 4423 4455 4497 Size: 32 135 32 1047 32 1772 32 42 Cuts at: 4497 4529 1373 Size: 32 2626 Fragments arranged by size: 2626 1772 1047 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2163 2701 2971 3126 3372 669 Size: 988 281 225 538 270 155 246 3079 Fragments arranged by size: 3079 988 538 281 270 246 225 155 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4896 5080 1305 Size: 3591 184 2007 Fragments arranged by size: 3591 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3740 4099 4258 518 Size: 648 2574 359 159 2042 Fragments arranged by size: 2574 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2258 2838 3198 3875 5023 5268 1606 Size: 652 580 360 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 652 580 360 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5230 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 2169 3140 4685 1132 Size: 97 940 971 1545 2229 Fragments arranged by size: 2229 1545 971 940 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5782 PciI A'CATG_T Cuts at: 2165 4681 2165 Size: 2516 3266 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2390 2390 Size: 5782 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5782 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PspOMI G'GGCC_C Cuts at: 2279 2279 Size: 5782 PstI C_TGCA'G Cuts at: 830 2349 2385 3134 830 Size: 1519 36 749 3478 Fragments arranged by size: 3478 1519 749 36 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2258 2838 2258 Size: 580 5202 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 2132 4030 5742 2132 Size: 1898 1712 2172 Fragments arranged by size: 2172 1898 1712 SfcI C'TryA_G Cuts at: 826 1045 2294 2345 2381 2778 3130 4265 4946 Size: 219 1249 51 36 397 352 1135 681 Cuts at: 4946 5137 826 Size: 191 1471 Fragments arranged by size: 1471 1249 1135 681 397 352 219 191 51 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2873 3886 4787 5049 820 Size: 197 926 930 1013 901 262 1553 Fragments arranged by size: 1553 1013 930 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 2597 1517 Size: 1080 4702 StyI C'CwwG_G Cuts at: 513 1065 2010 2998 3039 3221 513 Size: 552 945 988 41 182 3074 Fragments arranged by size: 3074 988 945 552 182 41 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3809 3826 3979 4164 4609 325 Size: 1255 2229 17 153 185 445 1498 Fragments arranged by size: 2229 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2130 2506 2739 Size: 274 80 33 51 1443 153 376 233 Cuts at: 2739 4028 5740 96 Size: 1289 1712 138 Fragments arranged by size: 1712 1443 1289 376 274 233 153 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5742 5774 5742 Size: 32 5750 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5782 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 2438 3911 2438 Size: 1473 4309 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI