################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII Acc65I AccIII AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BfrI BglI BlnI BsaAI BsaBI BseAI BseRI BsiEI BsiWI BsmBI BsmFI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CfoI Cfr10I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoT22I FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI RcaI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 280 GTCTAC GT^[AC][GT]AC AccII 203 CGCG CG^CG AcyI 1411 GGCGTC G[GA]^CG[CT]C AflIII 2450 ACGTGT A^C[GA][CT]GT AlwNI 1653 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 424 GGGCCC GGGCC^C AseI 1732 ATTAAT AT^TAAT AsnI 1732 ATTAAT AT^TAAT AspHI 2049 GAGCAC G[AT]GC[AT]^C AvaI 1385 CCCGAG C^[CT]CG[GA]G BalI 2285 TGGCCA TGG^CCA BanII 424 GGGCCC G[GA]GC[CT]^C BciVI 1557 GGATAC GTATCC(6/5) BclI 1466 TGATCA T^GATCA BglII 657 AGATCT A^GATCT BlpI 831 GCTTAGC GC^T[ACGT]AGC BpmI 596 CTCCAG CTGGAG(16/14) Bpu1102I 831 GCTTAGC GC^T[ACGT]AGC BsaHI 1411 GGCGTC G[GA]^CG[CT]C BsaWI 1405 ACCGGA [AT]^CCGG[AT] BsgI 1805 GTGCAG GTGCAG(16/14) BsiHKAI 2049 GAGCAC G[AT]GC[AT]^C BsoBI 1385 CCCGAG C^[CT]CG[GA]G BsrBI 2291 CCGCTC CCGCTC(-3/-3) BssSI 1530 CACGAG CACGAG(-5/-1) BstUI 203 CGCG CG^CG BstXI 138 CCATAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 657 AGATCT [GA]^GATC[CT] CelII 831 GCTTAGC GC^T[ACGT]AGC DraII 424 GGGCCCT [GA]G^G[ACGT]CC[CT] DrdI 271 GACAAGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 2285 TGGCCA [CT]^GGCC[GA] EcoNI 428 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 424 GGGCCCT [GA]G^G[ACGT]CC[CT] EcoRV 1147 GATATC GAT^ATC FbaI 1466 TGATCA T^GATCA Hin1I 1411 GGCGTC G[GA]^CG[CT]C MflI 657 AGATCT [GA]^GATC[CT] MluNI 2285 TGGCCA TGG^CCA MscI 2285 TGGCCA TGG^CCA MspA1I 946 CAGCTG C[AC]G^C[GT]G MvnI 203 CGCG CG^CG NcoI 746 CCATGG C^CATGG NspI 1368 GCATGC [GA]CATG^[CT] PshBI 1732 ATTAAT AT^TAAT PvuII 946 CAGCTG CAG^CTG SapI 804 GAAGAGC GCTCTTC(1/4) ScaI 2423 AGTACT AGT^ACT SexAI 515 ACCAGGT A^CC[AT]GGT SpeI 171 ACTAGT A^CTAGT SphI 1368 GCATGC GCATG^C Tsp45I 1655 GTGAC ^GT[GC]AC XhoII 657 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AclI 1003 AACGTT AA^CGTT 1012 AACGTT AlwI 530 GGATC GGATC(4/5) 2240 GGATC AocI 85 CCTAAGG CC^T[ACGT]AGG 432 CCTGAGG Asp700I 1701 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2435 GAATGGATTC AvaII 148 GGACC G^G[AT]CC 1041 GGTCC BcnI 527 CCCGG CC^[GC]GG 1548 CCGGG BmyI 424 GGGCCC G[AGT]GC[ACT]^C 2049 GAGCAC BsmI 158 GCATTC GAATGC(1/-1) 1195 GCATTC Bsp1286I 424 GGGCCC G[AGT]GC[ACT]^C 2049 GAGCAC Bsu36I 85 CCTAAGG CC^T[ACGT]AGG 432 CCTGAGG DsaI 746 CCATGG C^C[GA][CT]GG 983 CCGTGG Eco81I 85 CCTAAGG CC^T[ACGT]AGG 432 CCTGAGG EcoRI 692 GAATTC G^AATTC 1131 GAATTC EcoT14I 638 CCAAGG C^C[AT][AT]GG 746 CCATGG FauI 1897 CCCGC CCCGC(4/6) 41 GCGGG MslI 1268 CATACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2521 CATGTATATG NciI 527 CCCGG CC^[GC]GG 1548 CCGGG NdeI 248 CATATG CA^TATG 1925 CATATG Psp1406I 1003 AACGTT AA^CGTT 1012 AACGTT PstI 1696 CTGCAG CTGCA^G 2233 CTGCAG StyI 638 CCAAGG C^C[AT][AT]GG 746 CCATGG XbaI 1932 TCTAGA T^CTAGA 2295 TCTAGA XcmI 770 CCAAAGAGGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2477 CCATGAAAAATATGG XmnI 1701 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2435 GAATGGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbsI BbvI BfaI BpuAI BsaI BsaJI BsiYI BslI BsmAI BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I