(Circular) (Six-base) MAPSORT of: pFN21AE0018.seq Check: 2760 from: 1 to: 7273 pFN21AE0018 7273 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3708 3708 Size: 7273 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5083 278 Size: 53 83 186 936 3547 2468 Fragments arranged by size: 3547 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2286 4616 1937 Size: 349 2330 4594 Fragments arranged by size: 4594 2330 349 Acc65I G'GTAC_C Cuts at: 4594 4594 Size: 7273 AclI AA'CG_TT Cuts at: 1563 3009 3018 5400 5773 1563 Size: 1446 9 2382 373 3063 Fragments arranged by size: 3063 2382 1446 373 9 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3800 3815 5336 6720 6988 1632 Size: 2168 15 1521 1384 268 1917 Fragments arranged by size: 2168 1917 1521 1384 268 15 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7076 AflIII A'CryG_T Cuts at: 1203 4455 4570 6172 7149 1203 Size: 3252 115 1602 977 1327 Fragments arranged by size: 3252 1602 1327 977 115 AgeI A'CCGG_T Cuts at: 6104 6104 Size: 7273 AhdI GACnn_n'nnGTC Cuts at: 6002 6002 Size: 7273 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7273 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7273 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2639 2671 2639 Size: 32 7241 AlwNI CAG_nnn'CTG Cuts at: 3663 5016 6588 3663 Size: 1353 1572 4348 Fragments arranged by size: 4348 1572 1353 ApaI G_GGCC'C Cuts at: 2433 2433 Size: 7273 ApaLI G'TGCA_C Cuts at: 5330 6486 5330 Size: 1156 6117 ApoI r'AATT_y Cuts at: 1493 2237 2697 3136 3244 3970 4351 4582 4808 Size: 744 460 439 108 726 381 231 226 Cuts at: 4808 4960 7069 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 744 726 460 439 381 231 226 152 108 AseI AT'TA_AT Cuts at: 160 1032 3738 5827 160 Size: 872 2706 2089 1606 Fragments arranged by size: 2706 2089 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7273 AvaI C'yCGr_G Cuts at: 1870 1943 3390 4598 1870 Size: 73 1447 1208 4545 Fragments arranged by size: 4545 1447 1208 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4296 4329 4296 Size: 33 7240 BamHI G'GATC_C Cuts at: 4603 4970 4603 Size: 367 6906 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4594 6049 618 Size: 325 217 627 2807 1455 1842 Fragments arranged by size: 2807 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2433 4592 1391 Size: 1042 2159 4072 Fragments arranged by size: 4072 2159 1042 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3167 3412 3591 928 Size: 971 1268 245 179 4610 Fragments arranged by size: 4610 1268 971 245 179 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5464 5498 1903 Size: 34 3527 34 3678 Fragments arranged by size: 3678 3527 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3556 5165 6375 3556 Size: 1609 1210 4454 Fragments arranged by size: 4454 1609 1210 BclI T'GATC_A Cuts at: 1548 3471 1548 Size: 1923 5350 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5884 136 Size: 107 122 71 631 605 4212 1525 Fragments arranged by size: 4212 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2662 7268 2662 Size: 4606 2667 BlpI GC'TnA_GC Cuts at: 2837 4690 2837 Size: 1853 5420 Bme1580I G_kGCm'C Cuts at: 1790 2433 5334 6490 1790 Size: 643 2901 1156 2573 Fragments arranged by size: 2901 2573 1156 643 BmgBI CAC'GTC Cuts at: 1318 4456 1318 Size: 3138 4135 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2623 3598 5004 Size: 416 53 83 399 24 1206 975 1406 Cuts at: 5004 5962 442 Size: 958 1753 Fragments arranged by size: 1753 1406 1206 975 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7273 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2586 5933 1239 Size: 1347 3347 2579 Fragments arranged by size: 3347 2579 1347 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5398 6263 6561 5398 Size: 865 298 6110 Fragments arranged by size: 6110 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2123 2707 2765 5936 882 Size: 617 624 584 58 3171 2219 Fragments arranged by size: 3171 2219 624 617 584 58 BsaAI yAC'GTr Cuts at: 493 1204 5064 493 Size: 711 3860 2702 Fragments arranged by size: 3860 2702 711 BsaBI GATnn'nnATC Cuts at: 7267 7267 Size: 7273 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3417 5080 5462 6097 Size: 53 83 186 936 1884 1663 382 635 Cuts at: 6097 275 Size: 1451 Fragments arranged by size: 1884 1663 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3410 5706 6104 6378 6525 6936 3410 Size: 2296 398 274 147 411 3747 Fragments arranged by size: 3747 2296 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2071 2101 4442 4472 1178 Size: 30 165 30 668 30 2341 30 3979 Fragments arranged by size: 3979 2341 668 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7273 BseYI C'CCAG_C Cuts at: 2035 3283 6476 6972 2035 Size: 1248 3193 496 2336 Fragments arranged by size: 3193 2336 1248 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3831 1163 Size: 573 2095 4605 Fragments arranged by size: 4605 2095 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4723 5484 6512 1540 Size: 275 186 2722 761 1028 2301 Fragments arranged by size: 2722 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4058 5334 5419 6490 4058 Size: 1276 85 1071 4841 Fragments arranged by size: 4841 1276 1071 85 BsmI GAATG_Cn' Cuts at: 2163 3200 4784 4877 2163 Size: 1037 1584 93 4559 Fragments arranged by size: 4559 1584 1037 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2433 4058 4592 5334 5419 6490 1391 Size: 399 643 1625 534 742 85 1071 2174 Fragments arranged by size: 2174 1625 1071 742 643 534 399 85 BspEI T'CCGG_A Cuts at: 6936 6936 Size: 7273 BspHI T'CATG_A Cuts at: 5162 6892 5162 Size: 1730 5543 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2930 3107 3708 4628 844 Size: 2086 177 601 920 3489 Fragments arranged by size: 3489 2086 920 601 177 BsrBI CCG'CTC Cuts at: 1394 1823 4298 5160 1394 Size: 429 2475 862 3507 Fragments arranged by size: 3507 2475 862 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5768 5942 65 Size: 1183 4520 174 1396 Fragments arranged by size: 4520 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5917 6104 1700 Size: 110 4107 187 2869 Fragments arranged by size: 4107 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7273 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7273 BssSI C'ACGA_G Cuts at: 3535 5333 6345 3535 Size: 1798 1012 4463 Fragments arranged by size: 4463 1798 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7273 BstBI TT'CG_AA Cuts at: 6077 6077 Size: 7273 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7273 BstXI CCAn_nnnn'nTGG Cuts at: 2150 2150 Size: 7273 BstYI r'GATC_y Cuts at: 1972 2662 4603 4970 5355 5372 6824 6910 6922 Size: 690 1941 367 385 17 1452 86 12 Cuts at: 6922 7268 1972 Size: 346 1977 Fragments arranged by size: 1977 1941 1452 690 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2091 2438 2091 Size: 347 6926 BtgI C'CryG_G Cuts at: 513 1065 2004 2751 2988 513 Size: 552 939 747 237 4798 Fragments arranged by size: 4798 939 747 552 237 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6534 Fragments arranged by size: 6534 683 56 BtsI GCAGTG_nn' Cuts at: 3769 4247 4789 5583 5603 3769 Size: 478 542 794 20 5439 Fragments arranged by size: 5439 794 542 478 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7273 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2377 2412 529 Size: 35 1813 35 5390 Fragments arranged by size: 5390 1813 35 35 DraI TTT'AAA Cuts at: 4577 4577 Size: 7273 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2282 6280 809 Size: 507 966 3998 1802 Fragments arranged by size: 3998 1802 966 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4290 4720 5609 Size: 54 1425 50 275 18 2460 430 889 Cuts at: 5609 7238 8 Size: 1629 43 Fragments arranged by size: 2460 1629 1425 889 430 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4720 1537 Size: 275 2908 4090 Fragments arranged by size: 4090 2908 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2804 3255 3699 5203 1353 Size: 1451 451 444 1504 3423 Fragments arranged by size: 3423 1504 1451 451 444 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2709 5860 6234 6380 387 Size: 1064 138 1120 3151 374 146 1280 Fragments arranged by size: 3151 1280 1120 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2586 3800 3815 5336 5933 6720 6988 Size: 393 954 1214 15 1521 597 787 268 Cuts at: 6988 1239 Size: 1524 Fragments arranged by size: 1524 1521 1214 954 787 597 393 268 15 EcoNI CCTnn'n_nnAGG Cuts at: 2437 2437 Size: 7273 EcoO109I rG'GnC_Cy Cuts at: 2430 2430 Size: 7273 EcoRI G'AATT_C Cuts at: 2697 3136 4582 4960 2697 Size: 439 1446 378 5010 Fragments arranged by size: 5010 1446 439 378 EcoRV GAT'ATC Cuts at: 3154 3154 Size: 7273 FspI TGC'GCA Cuts at: 5779 5779 Size: 7273 HaeII r_GCGC'y Cuts at: 1678 6420 1678 Size: 4742 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2706 2732 2738 2764 3179 Size: 32 135 32 1134 26 6 26 415 Cuts at: 3179 3211 3384 3416 5914 5946 5988 6020 1373 Size: 32 173 32 2498 32 42 32 2626 Fragments arranged by size: 2626 2498 1134 415 173 135 42 32 32 32 32 32 32 26 26 6 HincII GTy'rAC Cuts at: 669 1657 1938 4617 4863 669 Size: 988 281 2679 246 3079 Fragments arranged by size: 3079 2679 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7273 HpaI GTT'AAC Cuts at: 4863 4863 Size: 7273 KpnI G_GTAC'C Cuts at: 4598 4598 Size: 7273 MfeI C'AATT_G Cuts at: 4872 4872 Size: 7273 MluI A'CGCG_T Cuts at: 7149 7149 Size: 7273 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2528 2766 3073 3412 3418 3429 4086 6387 Size: 1223 238 307 339 6 11 657 2301 Cuts at: 6387 6571 1305 Size: 184 2007 Fragments arranged by size: 2301 2007 1223 657 339 307 238 184 11 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 4292 10 Size: 54 1425 343 2460 2991 Fragments arranged by size: 2991 2460 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3277 4530 5231 5590 5749 518 Size: 648 2111 1253 701 359 159 2042 Fragments arranged by size: 2111 2042 1253 701 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2953 4689 5366 6514 6759 1606 Size: 1347 1736 677 1148 245 2120 Fragments arranged by size: 2120 1736 1347 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2751 513 Size: 552 939 747 5035 Fragments arranged by size: 5035 939 747 552 NdeI CA'TA_TG Cuts at: 387 2254 3931 387 Size: 1867 1677 3729 Fragments arranged by size: 3729 1867 1677 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7273 NotI GC'GGCC_GC Cuts at: 4720 4720 Size: 7273 NspI r_CATG'y Cuts at: 1132 1229 3377 4574 4631 6176 1132 Size: 97 2148 1197 57 1545 2229 Fragments arranged by size: 2229 2148 1545 1197 97 57 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7273 PciI A'CATG_T Cuts at: 4570 6172 4570 Size: 1602 5671 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7273 PmeI GTTT'AAAC Cuts at: 4577 4577 Size: 7273 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5342 5374 6855 6887 5342 Size: 32 1481 32 5728 Fragments arranged by size: 5728 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7273 PsiI TTA'TAA Cuts at: 4843 4843 Size: 7273 PspOMI G'GGCC_C Cuts at: 2429 2429 Size: 7273 PstI C_TGCA'G Cuts at: 830 3705 4242 4625 830 Size: 2875 537 383 3478 Fragments arranged by size: 3478 2875 537 383 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7273 PvuII CAG'CTG Cuts at: 2953 2953 Size: 7273 SalI G'TCGA_C Cuts at: 1936 4615 1936 Size: 2679 4594 SapI GCTCTTCn'nnn_ Cuts at: 2804 2804 Size: 7273 SbfI CC_TGCA'GG Cuts at: 4625 4625 Size: 7273 ScaI AGT'ACT Cuts at: 4430 5521 7233 4430 Size: 1091 1712 4470 Fragments arranged by size: 4470 1712 1091 SexAI A'CCwGG_T Cuts at: 2520 2520 Size: 7273 SfcI C'TryA_G Cuts at: 826 1045 3701 4038 4238 4621 5756 6437 6628 Size: 219 2656 337 200 383 1135 681 191 Cuts at: 6628 826 Size: 1471 Fragments arranged by size: 2656 1471 1135 681 383 337 219 200 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7273 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7273 SmaI CCC'GGG Cuts at: 1872 4600 1872 Size: 2728 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5377 6278 6540 820 Size: 197 926 3434 901 262 1553 Fragments arranged by size: 3434 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5064 493 Size: 4571 2702 SpeI A'CTAG_T Cuts at: 152 2176 152 Size: 2024 5249 SphI G_CATG'C Cuts at: 1132 3377 4631 1132 Size: 2245 1254 3774 Fragments arranged by size: 3774 2245 1254 SspI AAT'ATT Cuts at: 5 52 5197 5 Size: 47 5145 2081 Fragments arranged by size: 5145 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7273 StyI C'CwwG_G Cuts at: 513 1065 2004 2643 2751 4712 513 Size: 552 939 639 108 1961 3074 Fragments arranged by size: 3074 1961 939 639 552 108 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2721 3275 3797 5300 5317 5470 5655 Size: 1255 1141 554 522 1503 17 153 185 Cuts at: 5655 6100 325 Size: 445 1498 Fragments arranged by size: 1503 1498 1255 1141 554 522 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2631 2828 3088 Size: 274 80 33 51 1443 654 197 260 Cuts at: 3088 4428 5519 7231 96 Size: 1340 1091 1712 138 Fragments arranged by size: 1712 1443 1340 1091 654 274 260 197 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2071 2103 7233 7265 2071 Size: 32 5130 32 2079 Fragments arranged by size: 5130 2079 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7273 XbaI T'CTAG_A Cuts at: 3937 4300 4609 3937 Size: 363 309 6601 Fragments arranged by size: 6601 363 309 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2782 4489 2782 Size: 1707 5566 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7273 XmaI C'CCGG_G Cuts at: 1870 4598 1870 Size: 2728 4545 XmnI GAAnn'nnTTC Cuts at: 3710 4444 5402 3710 Size: 734 958 5581 Fragments arranged by size: 5581 958 734 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5081 276 Size: 53 83 186 936 3547 2468 Fragments arranged by size: 3547 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI