(Linear) (Six-base) MAPSORT of: KIEE6318.seq Check: 2659 from: 1 to: 1152 KIEE6318.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 150 1059 1152 Size: 150 909 93 Fragments arranged by size: 909 150 93 AccI GT'mk_AC Cuts at: 0 287 1152 Size: 287 865 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 688 742 1152 Size: 688 54 410 Fragments arranged by size: 688 410 54 AflIII A'CryG_T Cuts at: 0 194 356 374 1152 Size: 194 162 18 778 Fragments arranged by size: 778 194 162 18 AleI CACnn'nnGTG Cuts at: 0 758 1152 Size: 758 394 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 485 1152 Size: 485 667 AlwNI CAG_nnn'CTG Cuts at: 0 438 666 717 1152 Size: 438 228 51 435 Fragments arranged by size: 438 435 228 51 ApaI G_GGCC'C Cuts at: 0 1097 1152 Size: 1097 55 ApaLI G'TGCA_C Cuts at: 0 241 352 1152 Size: 241 111 800 Fragments arranged by size: 800 241 111 AvaI C'yCGr_G Cuts at: 0 92 205 474 812 1152 Size: 92 113 269 338 340 Fragments arranged by size: 340 338 269 113 92 BamHI G'GATC_C Cuts at: 0 88 1152 Size: 88 1064 BanI G'GyrC_C Cuts at: 0 531 773 988 1152 Size: 531 242 215 164 Fragments arranged by size: 531 242 215 164 BanII G_rGCy'C Cuts at: 0 71 819 1097 1152 Size: 71 748 278 55 Fragments arranged by size: 748 278 71 55 BbvCI CC'TCA_GC Cuts at: 0 431 913 1152 Size: 431 482 239 Fragments arranged by size: 482 431 239 BclI T'GATC_A Cuts at: 0 683 1152 Size: 683 469 BlpI GC'TnA_GC Cuts at: 0 979 1152 Size: 979 173 Bme1580I G_kGCm'C Cuts at: 0 61 245 356 536 813 1097 1139 1152 Size: 61 184 111 180 277 284 42 13 Fragments arranged by size: 284 277 184 180 111 61 42 13 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 606 1152 Size: 606 546 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 694 769 844 892 898 943 1152 Size: 694 75 75 48 6 45 209 Fragments arranged by size: 694 209 75 75 48 45 6 Bpu10I CC'TnA_GC Cuts at: 0 431 505 913 1152 Size: 431 74 408 239 Fragments arranged by size: 431 408 239 74 BsaI GGTCTCn'nnnn_ Cuts at: 0 487 1152 Size: 487 665 BsaAI yAC'GTr Cuts at: 0 351 359 804 1152 Size: 351 8 445 348 Fragments arranged by size: 445 351 348 8 BsaWI w'CCGG_w Cuts at: 0 85 1152 Size: 85 1067 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 634 646 664 676 984 1014 1152 Size: 634 12 18 12 308 30 138 Fragments arranged by size: 634 308 138 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 145 598 745 964 967 1152 Size: 145 453 147 219 3 185 Fragments arranged by size: 453 219 185 147 145 3 BseYI C'CCAG_C Cuts at: 0 393 570 1152 Size: 393 177 582 Fragments arranged by size: 582 393 177 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 34 679 1152 Size: 34 645 473 Fragments arranged by size: 645 473 34 BsiHKAI G_wGCw'C Cuts at: 0 71 245 356 755 1152 Size: 71 174 111 399 397 Fragments arranged by size: 399 397 174 111 71 Bsp1286I G_dGCh'C Cuts at: 0 61 71 245 356 536 755 813 819 Size: 61 10 174 111 180 219 58 6 Cuts at: 819 1097 1139 1152 Size: 278 42 13 Fragments arranged by size: 278 219 180 174 111 61 58 42 13 10 6 BspHI T'CATG_A Cuts at: 0 513 1152 Size: 513 639 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 150 453 638 1059 1152 Size: 150 303 185 421 93 Fragments arranged by size: 421 303 185 150 93 BsrBI CCG'CTC Cuts at: 0 10 135 957 1152 Size: 10 125 822 195 Fragments arranged by size: 822 195 125 10 BssSI C'ACGA_G Cuts at: 0 244 1152 Size: 244 908 BstAPI GCAn_nnn'nTGC Cuts at: 0 1075 1143 1152 Size: 1075 68 9 Fragments arranged by size: 1075 68 9 BstYI r'GATC_y Cuts at: 0 88 218 1152 Size: 88 130 934 Fragments arranged by size: 934 130 88 Bsu36I CC'TnA_GG Cuts at: 0 310 622 1152 Size: 310 312 530 Fragments arranged by size: 530 312 310 BtgI C'CryG_G Cuts at: 0 543 649 1152 Size: 543 106 503 Fragments arranged by size: 543 503 106 BtsI GCAGTG_nn' Cuts at: 0 667 1152 Size: 667 485 DraIII CAC_nnn'GTG Cuts at: 0 249 344 1152 Size: 249 95 808 Fragments arranged by size: 808 249 95 EaeI y'GGCC_r Cuts at: 0 251 368 1152 Size: 251 117 784 Fragments arranged by size: 784 251 117 EarI CTCTTCn'nnn_ Cuts at: 0 568 597 1152 Size: 568 29 555 Fragments arranged by size: 568 555 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 868 952 1152 Size: 868 84 200 Fragments arranged by size: 868 200 84 EcoICRI GAG'CTC Cuts at: 0 69 1152 Size: 69 1083 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 688 694 742 769 844 892 898 943 Size: 688 6 48 27 75 48 6 45 Cuts at: 943 1152 Size: 209 Fragments arranged by size: 688 209 75 48 48 45 27 6 6 EcoO109I rG'GnC_Cy Cuts at: 0 333 405 1093 1094 1152 Size: 333 72 688 1 58 Fragments arranged by size: 688 333 72 58 1 FspI TGC'GCA Cuts at: 0 904 1152 Size: 904 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 675 707 1152 Size: 675 32 445 Fragments arranged by size: 675 445 32 MscI TGG'CCA Cuts at: 0 370 1152 Size: 370 782 MslI CAynn'nnrTG Cuts at: 0 238 758 1152 Size: 238 520 394 Fragments arranged by size: 520 394 238 NspI r_CATG'y Cuts at: 0 198 378 978 1152 Size: 198 180 600 174 Fragments arranged by size: 600 198 180 174 PasI CC'CwG_GG Cuts at: 0 324 1132 1152 Size: 324 808 20 Fragments arranged by size: 808 324 20 PciI A'CATG_T Cuts at: 0 194 374 1152 Size: 194 180 778 Fragments arranged by size: 778 194 180 PflMI CCAn_nnn'nTGG Cuts at: 0 344 393 1152 Size: 344 49 759 Fragments arranged by size: 759 344 49 PfoI T'CCnGG_A Cuts at: 0 301 1152 Size: 301 851 PmlI CAC'GTG Cuts at: 0 351 359 1152 Size: 351 8 793 Fragments arranged by size: 793 351 8 PpuMI rG'GwC_Cy Cuts at: 0 333 405 1152 Size: 333 72 747 Fragments arranged by size: 747 333 72 PshAI GACnn'nnGTC Cuts at: 0 152 1152 Size: 152 1000 PspOMI G'GGCC_C Cuts at: 0 1093 1152 Size: 1093 59 PspXI vC'TCGA_Gb Cuts at: 0 205 1152 Size: 205 947 PstI C_TGCA'G Cuts at: 0 422 464 635 1056 1080 1104 1148 1152 Size: 422 42 171 421 24 24 44 4 Fragments arranged by size: 422 421 171 44 42 24 24 4 SacI G_AGCT'C Cuts at: 0 71 1152 Size: 71 1081 SapI GCTCTTCn'nnn_ Cuts at: 0 568 1152 Size: 568 584 ScaI AGT'ACT Cuts at: 0 736 1152 Size: 736 416 SfcI C'TryA_G Cuts at: 0 418 460 631 1052 1076 1100 1144 1152 Size: 418 42 171 421 24 24 44 8 Fragments arranged by size: 421 418 171 44 42 24 24 8 SmaI CCC'GGG Cuts at: 0 814 1152 Size: 814 338 SmlI C'TyrA_G Cuts at: 0 205 1152 Size: 205 947 SrfI GCCC'GGGC Cuts at: 0 814 1152 Size: 814 338 StuI AGG'CCT Cuts at: 0 589 796 1152 Size: 589 207 356 Fragments arranged by size: 589 356 207 StyI C'CwwG_G Cuts at: 0 638 848 1152 Size: 638 210 304 Fragments arranged by size: 638 304 210 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 794 1029 1077 1152 Size: 794 235 48 75 Fragments arranged by size: 794 235 75 48 TatI w'GTAC_w Cuts at: 0 734 1152 Size: 734 418 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 80 112 634 646 666 678 984 1016 Size: 80 32 522 12 20 12 306 32 Cuts at: 1016 1152 Size: 136 Fragments arranged by size: 522 306 136 80 32 32 20 12 12 XhoI C'TCGA_G Cuts at: 0 205 1152 Size: 205 947 XmaI C'CCGG_G Cuts at: 0 812 1152 Size: 812 340 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbvCI BclI BlpI Bme1580I BplI BpmI Bpu10I BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrBI BssSI BstAPI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I FspI Hin4I MscI MslI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PstI SacI SapI ScaI SfcI SmaI SmlI SrfI StuI StyI TaqII TatI TstI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI BcgI BciVI BfrBI BglI BglII BmgBI BmrI BmtI BpuEI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoNI EcoRI EcoRV FalI FseI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PpiI PsiI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI Tth111I XbaI XcmI XmnI ZraI