################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AflII AgeI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspI AviII AvrII BalI BanI BbeI BbrPI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BspEI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI Bsu36I CelII Cfr10I CpoI DraI DraII DraIII DrdI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I MaeII MamI MfeI MluI MluNI MroI MscI MseI MspI MunI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tsp509I Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccI 669 GTATAC GT^[AC][GT]AC AccII 902 CGCG CG^CG AflIII 701 ACATGT A^C[GA][CT]GT AhdI 549 GACTCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 384 AGCGCT AGC^GCT Asp700I 774 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 549 GACTCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 800 GAGCTC G[AT]GC[AT]^C AvaI 641 CTCGAG C^[CT]CG[GA]G BbsI 132 GTCTTC GAAGAC(2/6) BbvCI 100 CCTCAGC CCTCAGC(-5/-2) BfaI 952 CTAG C^TAG BpmI 406 CTCCAG CTGGAG(16/14) BpuAI 132 GTCTTC GAAGAC(2/6) BsgI 85 CTGCAC GTGCAG(16/14) BsiHKAI 800 GAGCTC G[AT]GC[AT]^C BsiYI 655 CCAGTCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 655 CCAGTCTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmI 975 GAATGC GAATGC(1/-1) BsoBI 641 CTCGAG C^[CT]CG[GA]G Bsp1407I 884 TGTACA T^GTACA BspDI 488 ATCGAT AT^CGAT BspHI 435 TCATGA T^CATGA BspLU11I 701 ACATGT A^CATGT BspMI 83 ACCTGC ACCTGC(4/8) BsrGI 884 TGTACA T^GTACA BssKI 45 CCAGG ^CC[ACGT]GG Bst1107I 669 GTATAC GTA^TAC BstNI 45 CCAGG CC^[AT]GG BstUI 902 CGCG CG^CG BstXI 371 CCAGCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 669 GTATAC GTA^TAC ClaI 488 ATCGAT AT^CGAT EaeI 59 TGGCCG [CT]^GGCC[GA] Eam1105I 549 GACTCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 384 AGCGCT AGC^GCT EcoRII 45 CCAGG ^CC[AT]GG EcoT14I 962 CCATGG C^C[AT][AT]GG EcoT22I 977 ATGCAT ATGCA^T KspI 901 CCGCGG CCGC^GG MaeI 952 CTAG C^TAG MslI 436 CATGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 901 CCGCGG C[AC]G^C[GT]G MvaI 45 CCAGG CC^[AT]GG MvnI 902 CGCG CG^CG NcoI 962 CCATGG C^CATGG NsiI 977 ATGCAT ATGCA^T NspI 701 ACATGT [GA]CATG^[CT] PaeR7I 641 CTCGAG C^TCGAG PflMI 655 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 435 TCATGA T^CATGA SacI 800 GAGCTC GAGCT^C SacII 901 CCGCGG CCGC^GG ScrFI 45 CCAGG CC^[ACGT]GG SfcI 471 CTACAG C^T[GA][CT]AG SspBI 884 TGTACA T^GTACA StyI 962 CCATGG C^C[AT][AT]GG Van91I 655 CCAGTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 641 CTCGAG C^TCGAG XmnI 774 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AvaII 42 GGTCC G^G[AT]CC 905 GGTCC BanII 403 GGGCTC G[GA]GC[CT]^C 800 GAGCTC BsaI 339 GAGACC GGTCTC(1/5) 646 GGTCTC BsaJI 901 CCGCGG C^C[ACGT][ACGT]GG 962 CCATGG BsmFI 159 GGGAC GGGAC(10/14) 698 GGGAC BsrI 251 ACTGG ACTGG(1/-1) 655 CCAGT DsaI 901 CCGCGG C^C[GA][CT]GG 962 CCATGG HaeII 384 AGCGCT [GA]GCGC^[CT] 788 GGCGCT SfaNI 374 GCATC GCATC(5/9) 396 GCATC SmlI 279 CTTGAG C^T[CT][GA]AG 641 CTCGAG TfiI 486 GAATC G^A[AT]TC 918 GATTC Tsp45I 329 GTGAC ^GT[GC]AC 600 GTCAC TspRI 327 CAGTG CA[GC]TG[ACGT][ACGT]^ 911 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 41 enzymes) # ######################################### AciI AfaI AluI AlwI BamHI BbvI BmyI BsmAI Bsp1286I BstF5I BstYI Cac8I CfoI Cfr13I DdeI DpnII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeIII MboI MboII MflI MnlI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I TaqI TseI TthHB8I XhoII