################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaI BseAI BsgI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII Eam1105I EarI Eco47III Eco81I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI Ksp632I MaeI MamI MfeI MluI MroI MseI MunI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccI 1123 GTCTAC GT^[AC][GT]AC AcsI 1038 GAATTT [GA]^AATT[CT] AfaI 231 GTAC GT^AC AflIII 348 ACACGT A^C[GA][CT]GT ApoI 1038 GAATTT [GA]^AATT[CT] BamHI 516 GGATCC G^GATCC BbrPI 349 CACGTG CAC^GTG BbsI 344 GAAGAC GAAGAC(2/6) BpuAI 344 GAAGAC GAAGAC(2/6) BsaAI 349 CACGTG [CT]AC^GT[GA] BsaWI 584 ACCGGA [AT]^CCGG[AT] BsrBI 1320 CCGCTC CCGCTC(-3/-3) BsrDI 905 GCAATG GCAATG(2/0) BsrFI 284 GCCGGC [GA]^CCGG[CT] BstEII 802 GGTGACC G^GT[ACGT]ACC BstPI 802 GGTGACC G^GT[ACGT]ACC Cfr10I 284 GCCGGC [GA]^CCGG[CT] DrdI 588 GACATATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 522 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 802 GGTGACC G^GT[ACGT]ACC KspI 153 CCGCGG CCGC^GG MslI 815 CACCTGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 284 GCCGGC GCC^GGC NdeI 590 CATATG CA^TATG NgoMIV 284 GCCGGC G^CCGGC NotI 197 GCGGCCGC GC^GGCCGC PmaCI 349 CACGTG CAC^GTG PmlI 349 CACGTG CAC^GTG PpuMI 937 GGGACCC [GA]G^G[AT]CC[CT] PvuII 1353 CAGCTG CAG^CTG RsaI 231 GTAC GT^AC SacII 153 CCGCGG CCGC^GG SfaNI 1395 GCATC GCATC(5/9) SmaI 42 CCCGGG CCC^GGG SphI 1223 GCATGC GCATG^C Tsp509I 1039 AATT ^AATT XmaCI 42 CCCGGG C^CCGGG XmaI 42 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AvaI 42 CCCGGG C^[CT]CG[GA]G 71 CCCGAG BlpI 431 GCTCAGC GC^T[ACGT]AGC 1146 GCTGAGC Bpu1102I 431 GCTCAGC GC^T[ACGT]AGC 1146 GCTGAGC BsiEI 156 CGGCCG CG[GA][CT]^CG 198 CGGCCG BsmAI 481 GAGAC GTCTC(1/5) 1054 GAGAC BsoBI 42 CCCGGG C^[CT]CG[GA]G 71 CCCGAG BstXI 791 CCAAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 814 CCACCTGAATGG CelII 431 GCTCAGC GC^T[ACGT]AGC 1146 GCTGAGC EagI 156 CGGCCG C^GGCCG 198 CGGCCG EclXI 156 CGGCCG C^GGCCG 198 CGGCCG Eco52I 156 CGGCCG C^GGCCG 198 CGGCCG HgaI 171 GACGC GACGC(5/10) 203 GCGTC MaeII 350 ACGT A^CGT 1328 ACGT MspA1I 153 CCGCGG C[AC]G^C[GT]G 1353 CAGCTG NcoI 275 CCATGG C^CATGG 1427 CCATGG SacI 337 GAGCTC GAGCT^C 964 GAGCTC StuI 89 AGGCCT AGG^CCT 992 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AcyI AluI AlwI AlwNI AspHI AvaII BalI BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeIII MboI MboII MflI MluNI MnlI MscI MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI Sau3AI Sau96I ScrFI SfcI SfoI StyI TseI Tsp45I TspRI Van91I XcmI XhoII