(Circular) (Six-base) MAPSORT of: pFN21AE5601.seq Check: 250 from: 1 to: 6157 pFN21AE5601 6157 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3967 278 Size: 53 83 186 936 2431 2468 Fragments arranged by size: 2468 2431 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3129 3500 1937 Size: 1192 371 4594 Fragments arranged by size: 4594 1192 371 Acc65I G'GTAC_C Cuts at: 3478 3478 Size: 6157 AclI AA'CG_TT Cuts at: 1563 4284 4657 1563 Size: 2721 373 3063 Fragments arranged by size: 3063 2721 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4220 5604 5872 1632 Size: 2588 1384 268 1917 Fragments arranged by size: 2588 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5960 AflIII A'CryG_T Cuts at: 1203 2353 5056 6033 1203 Size: 1150 2703 977 1327 Fragments arranged by size: 2703 1327 1150 977 AgeI A'CCGG_T Cuts at: 4988 4988 Size: 6157 AhdI GACnn_n'nnGTC Cuts at: 4886 4886 Size: 6157 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6157 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6157 AlwNI CAG_nnn'CTG Cuts at: 2026 2405 2674 3172 3900 5472 2026 Size: 379 269 498 728 1572 2711 Fragments arranged by size: 2711 1572 728 498 379 269 ApaLI G'TGCA_C Cuts at: 4214 5370 4214 Size: 1156 5001 ApoI r'AATT_y Cuts at: 1493 3043 3466 3692 3844 5953 1493 Size: 1550 423 226 152 2109 1697 Fragments arranged by size: 2109 1697 1550 423 226 152 AseI AT'TA_AT Cuts at: 160 1032 4711 160 Size: 872 3679 1606 Fragments arranged by size: 3679 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6157 AvaI C'yCGr_G Cuts at: 1870 1943 2047 2076 3482 1870 Size: 73 104 29 1406 4545 Fragments arranged by size: 4545 1406 104 73 29 BamHI G'GATC_C Cuts at: 2521 3487 3854 2521 Size: 966 367 4824 Fragments arranged by size: 4824 966 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2125 2622 3478 4933 Size: 325 217 627 221 117 497 856 1455 Cuts at: 4933 618 Size: 1842 Fragments arranged by size: 1842 1455 856 627 497 325 221 217 117 BanII G_rGCy'C Cuts at: 1391 2346 2415 2438 2973 3476 1391 Size: 955 69 23 535 503 4072 Fragments arranged by size: 4072 955 535 503 69 23 BbeI G_GCGC'C Cuts at: 2012 2129 2626 2012 Size: 117 497 5543 Fragments arranged by size: 5543 497 117 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2356 928 Size: 971 457 4729 Fragments arranged by size: 4729 971 457 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4348 4382 1903 Size: 34 2411 34 3678 Fragments arranged by size: 3678 2411 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4049 5259 4049 Size: 1210 4947 BclI T'GATC_A Cuts at: 1548 1548 Size: 6157 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2220 3032 3185 Size: 107 122 71 631 605 548 812 153 Cuts at: 3185 3443 4768 136 Size: 258 1325 1525 Fragments arranged by size: 1525 1325 812 631 605 548 258 153 122 107 71 BglII A'GATC_T Cuts at: 6152 6152 Size: 6157 BlpI GC'TnA_GC Cuts at: 2437 3152 3574 2437 Size: 715 422 5020 Fragments arranged by size: 5020 715 422 Bme1580I G_kGCm'C Cuts at: 1790 3368 4218 5374 1790 Size: 1578 850 1156 2573 Fragments arranged by size: 2573 1578 1156 850 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6157 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2375 3382 3888 Size: 416 53 83 399 24 958 1007 506 Cuts at: 3888 4846 442 Size: 958 1753 Fragments arranged by size: 1753 1007 958 958 506 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6157 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 2264 2276 2854 3077 4817 1239 Size: 926 99 12 578 223 1740 2579 Fragments arranged by size: 2579 1740 926 578 223 99 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4282 5147 5445 4282 Size: 865 298 4994 Fragments arranged by size: 4994 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4820 882 Size: 617 3321 2219 Fragments arranged by size: 3321 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2356 3948 493 Size: 711 1152 1592 2702 Fragments arranged by size: 2702 1592 1152 711 BsaBI GATnn'nnATC Cuts at: 6151 6151 Size: 6157 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2126 2623 3964 Size: 53 83 186 936 476 117 497 1341 Cuts at: 3964 4346 4981 275 Size: 382 635 1451 Fragments arranged by size: 1451 1341 936 635 497 476 382 186 117 83 53 BsaWI w'CCGG_w Cuts at: 2589 4590 4988 5262 5409 5820 2589 Size: 2001 398 274 147 411 2926 Fragments arranged by size: 2926 2001 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2402 2432 2909 2939 3236 Size: 30 165 30 999 30 477 30 297 Cuts at: 3236 3266 1178 Size: 30 4069 Fragments arranged by size: 4069 999 477 297 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2273 2741 2744 2851 1186 Size: 1087 468 3 107 4492 Fragments arranged by size: 4492 1087 468 107 3 BseYI C'CCAG_C Cuts at: 2035 2070 2188 2405 2889 3360 5360 5856 2035 Size: 35 118 217 484 471 2000 496 2336 Fragments arranged by size: 2336 2000 496 484 471 217 118 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5584 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2164 2206 3607 4368 5396 1540 Size: 275 186 163 42 1401 761 1028 2301 Fragments arranged by size: 2301 1401 1028 761 275 186 163 42 BsiHKAI G_wGCw'C Cuts at: 2346 2659 2973 4218 4303 5374 2346 Size: 313 314 1245 85 1071 3129 Fragments arranged by size: 3129 1245 1071 314 313 85 BsmI GAATG_Cn' Cuts at: 3668 3761 3668 Size: 93 6064 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2346 2415 2438 2659 2973 3368 3476 Size: 399 556 69 23 221 314 395 108 Cuts at: 3476 4218 4303 5374 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 556 399 395 314 221 108 85 69 23 BspEI T'CCGG_A Cuts at: 5820 5820 Size: 6157 BspHI T'CATG_A Cuts at: 4046 5776 4046 Size: 1730 4427 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3512 844 Size: 2668 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3327 4044 1394 Size: 429 1504 717 3507 Fragments arranged by size: 3507 1504 717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2917 4652 4826 65 Size: 1183 1669 1735 174 1396 Fragments arranged by size: 1735 1669 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2289 4801 4988 1700 Size: 110 479 2512 187 2869 Fragments arranged by size: 2869 2512 479 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6157 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6157 BssSI C'ACGA_G Cuts at: 4217 5229 4217 Size: 1012 5145 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6157 BstBI TT'CG_AA Cuts at: 4961 4961 Size: 6157 BstEII G'GTnAC_C Cuts at: 1184 2807 1184 Size: 1623 4534 BstXI CCAn_nnnn'nTGG Cuts at: 2803 2826 2803 Size: 23 6134 BstYI r'GATC_y Cuts at: 1972 2521 2664 3249 3487 3854 4239 4256 5708 Size: 549 143 585 238 367 385 17 1452 Cuts at: 5708 5794 5806 6152 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 585 549 385 367 346 238 143 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2158 2280 2680 3144 3207 3244 Size: 552 939 154 122 400 464 63 37 Cuts at: 3244 3432 513 Size: 188 3238 Fragments arranged by size: 3238 939 552 464 400 188 154 122 63 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3386 487 Size: 683 56 2160 3258 Fragments arranged by size: 3258 2160 683 56 BtsI GCAGTG_nn' Cuts at: 2061 3673 4467 4487 2061 Size: 1612 794 20 3731 Fragments arranged by size: 3731 1612 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6157 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3189 3224 529 Size: 35 2625 35 3462 Fragments arranged by size: 3462 2625 35 35 DraI TTT'AAA Cuts at: 3461 3461 Size: 6157 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2599 5164 809 Size: 507 1283 2565 1802 Fragments arranged by size: 2565 1802 1283 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2161 2203 Size: 54 1425 50 275 18 228 103 42 Cuts at: 2203 3033 3186 3210 3435 3604 4493 6122 8 Size: 830 153 24 225 169 889 1629 43 Fragments arranged by size: 1629 1425 889 830 275 228 225 169 153 103 54 50 43 42 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2161 2203 3604 1537 Size: 275 349 42 1401 4090 Fragments arranged by size: 4090 1401 349 275 42 EarI CTCTTCn'nnn_ Cuts at: 1353 4087 1353 Size: 2734 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2201 4744 5118 5264 387 Size: 1064 138 612 2543 374 146 1280 Fragments arranged by size: 2543 1280 1064 612 374 146 138 EcoICRI GAG'CTC Cuts at: 2344 2971 2344 Size: 627 5530 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2264 2276 2854 3077 4220 4817 Size: 393 533 99 12 578 223 1143 597 Cuts at: 4817 5604 5872 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1143 787 597 578 533 393 268 223 99 12 EcoNI CCTnn'n_nnAGG Cuts at: 2531 2531 Size: 6157 EcoO109I rG'GnC_Cy Cuts at: 2671 2943 3414 2671 Size: 272 471 5414 Fragments arranged by size: 5414 471 272 EcoRI G'AATT_C Cuts at: 3466 3844 3466 Size: 378 5779 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2440 2440 Size: 6157 FspI TGC'GCA Cuts at: 4663 4663 Size: 6157 HaeII r_GCGC'y Cuts at: 1678 2012 2129 2626 5304 1678 Size: 334 117 497 2678 2531 Fragments arranged by size: 2678 2531 497 334 117 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2402 2434 2437 2469 2513 Size: 32 135 32 830 32 3 32 44 Cuts at: 2513 2545 3186 3218 4798 4830 4872 4904 1373 Size: 32 641 32 1580 32 42 32 2626 Fragments arranged by size: 2626 1580 830 641 135 44 42 32 32 32 32 32 32 32 32 3 HincII GTy'rAC Cuts at: 669 1657 1938 3501 3747 669 Size: 988 281 1563 246 3079 Fragments arranged by size: 3079 1563 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6157 HpaI GTT'AAC Cuts at: 3747 3747 Size: 6157 KasI G'GCGC_C Cuts at: 2008 2125 2622 2008 Size: 117 497 5543 Fragments arranged by size: 5543 497 117 KpnI G_GTAC'C Cuts at: 3482 3482 Size: 6157 MfeI C'AATT_G Cuts at: 3756 3756 Size: 6157 MluI A'CGCG_T Cuts at: 6033 6033 Size: 6157 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5271 5455 1305 Size: 3966 184 2007 Fragments arranged by size: 3966 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3188 3212 3437 10 Size: 54 1425 343 1356 24 225 2730 Fragments arranged by size: 2730 1425 1356 343 225 54 24 MslI CAynn'nnrTG Cuts at: 518 1166 2824 4115 4474 4633 518 Size: 648 1658 1291 359 159 2042 Fragments arranged by size: 2042 1658 1291 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2160 3360 3573 4250 5398 5643 1606 Size: 554 1200 213 677 1148 245 2120 Fragments arranged by size: 2120 1200 1148 677 554 245 213 NaeI GCC'GGC Cuts at: 2291 2291 Size: 6157 NarI GG'CG_CC Cuts at: 2009 2126 2623 2009 Size: 117 497 5543 Fragments arranged by size: 5543 497 117 NcoI C'CATG_G Cuts at: 513 1065 2004 2280 3432 513 Size: 552 939 276 1152 3238 Fragments arranged by size: 3238 1152 939 552 276 NdeI CA'TA_TG Cuts at: 387 2596 387 Size: 2209 3948 NgoMIV G'CCGG_C Cuts at: 2289 2289 Size: 6157 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6157 NotI GC'GGCC_GC Cuts at: 2203 3604 2203 Size: 1401 4756 NspI r_CATG'y Cuts at: 1132 1229 2878 3094 3232 3515 5060 1132 Size: 97 1649 216 138 283 1545 2229 Fragments arranged by size: 2229 1649 1545 283 216 138 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6157 PciI A'CATG_T Cuts at: 5056 5056 Size: 6157 PflMI CCAn_nnn'nTGG Cuts at: 1108 2335 2405 2674 1108 Size: 1227 70 269 4591 Fragments arranged by size: 4591 1227 269 70 PfoI T'CCnGG_A Cuts at: 2334 2334 Size: 6157 PmeI GTTT'AAAC Cuts at: 3461 3461 Size: 6157 PmlI CAC'GTG Cuts at: 2356 2356 Size: 6157 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2717 2749 2955 2987 4226 4258 5739 5771 2717 Size: 32 206 32 1239 32 1481 32 3103 Fragments arranged by size: 3103 1481 1239 206 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2943 2943 Size: 6157 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6157 PsiI TTA'TAA Cuts at: 3727 3727 Size: 6157 PstI C_TGCA'G Cuts at: 830 2022 2324 3278 3509 830 Size: 1192 302 954 231 3478 Fragments arranged by size: 3478 1192 954 302 231 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6157 PvuII CAG'CTG Cuts at: 3360 3360 Size: 6157 SacI G_AGCT'C Cuts at: 2346 2973 2346 Size: 627 5530 SacII CC_GC'GG Cuts at: 2161 2161 Size: 6157 SalI G'TCGA_C Cuts at: 1936 3499 1936 Size: 1563 4594 SanDI GG'GwC_CC Cuts at: 2943 2943 Size: 6157 SbfI CC_TGCA'GG Cuts at: 3509 3509 Size: 6157 ScaI AGT'ACT Cuts at: 4405 6117 4405 Size: 1712 4445 SfcI C'TryA_G Cuts at: 826 1045 2018 2320 3073 3274 3505 4640 5321 Size: 219 973 302 753 201 231 1135 681 Cuts at: 5321 5512 826 Size: 191 1471 Fragments arranged by size: 1471 1135 973 753 681 302 231 219 201 191 SfoI GGC'GCC Cuts at: 2010 2127 2624 2010 Size: 117 497 5543 Fragments arranged by size: 5543 497 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6157 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6157 SmaI CCC'GGG Cuts at: 1872 2049 3484 1872 Size: 177 1435 4545 Fragments arranged by size: 4545 1435 177 SmlI C'TyrA_G Cuts at: 820 1017 1943 4261 5162 5424 820 Size: 197 926 2318 901 262 1553 Fragments arranged by size: 2318 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3948 493 Size: 3455 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6157 SphI G_CATG'C Cuts at: 1132 3232 3515 1132 Size: 2100 283 3774 Fragments arranged by size: 3774 2100 283 SspI AAT'ATT Cuts at: 5 52 4081 5 Size: 47 4029 2081 Fragments arranged by size: 4029 2081 47 StuI AGG'CCT Cuts at: 1517 2096 2999 1517 Size: 579 903 4675 Fragments arranged by size: 4675 903 579 StyI C'CwwG_G Cuts at: 513 1065 2004 2280 2391 3123 3281 3432 3596 Size: 552 939 276 111 732 158 151 164 Cuts at: 3596 513 Size: 3074 Fragments arranged by size: 3074 939 732 552 276 164 158 151 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2795 4184 4201 4354 4539 4984 325 Size: 1255 1215 1389 17 153 185 445 1498 Fragments arranged by size: 1498 1389 1255 1215 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2235 4403 6115 Size: 274 80 33 51 1443 258 2168 1712 Cuts at: 6115 96 Size: 138 Fragments arranged by size: 2168 1712 1443 274 258 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2340 2359 2372 2380 2391 2412 2649 2681 2909 Size: 19 13 8 11 21 237 32 228 Cuts at: 2909 2941 3080 3112 3236 3268 6117 6149 2340 Size: 32 139 32 124 32 2849 32 2348 Fragments arranged by size: 2849 2348 237 228 139 124 32 32 32 32 32 21 19 13 11 8 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6157 XbaI T'CTAG_A Cuts at: 3493 3493 Size: 6157 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2876 3043 3445 2876 Size: 167 402 5588 Fragments arranged by size: 5588 402 167 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6157 XmaI C'CCGG_G Cuts at: 1870 2047 3482 1870 Size: 177 1435 4545 Fragments arranged by size: 4545 1435 177 XmnI GAAnn'nnTTC Cuts at: 4286 4286 Size: 6157 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3965 276 Size: 53 83 186 936 2431 2468 Fragments arranged by size: 2468 2431 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI NsiI PacI PspOMI PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI