(Linear) (Six-base) MAPSORT of: KIEE5601.seq Check: 7193 from: 1 to: 1452 KIEE5601.orf With 182 enzymes: SgfI * December 5, 2014 13:02 .. AccI GT'mk_AC Cuts at: 0 1124 1452 Size: 1124 328 AflIII A'CryG_T Cuts at: 0 348 1452 Size: 348 1104 AlwNI CAG_nnn'CTG Cuts at: 0 21 400 669 1167 1452 Size: 21 379 269 498 285 Fragments arranged by size: 498 379 285 269 21 ApoI r'AATT_y Cuts at: 0 1038 1452 Size: 1038 414 AvaI C'yCGr_G Cuts at: 0 42 71 1452 Size: 42 29 1381 Fragments arranged by size: 1381 42 29 BamHI G'GATC_C Cuts at: 0 516 1452 Size: 516 936 BanI G'GyrC_C Cuts at: 0 3 120 617 1452 Size: 3 117 497 835 Fragments arranged by size: 835 497 117 3 BanII G_rGCy'C Cuts at: 0 341 410 433 968 1452 Size: 341 69 23 535 484 Fragments arranged by size: 535 484 341 69 23 BbeI G_GCGC'C Cuts at: 0 7 124 621 1452 Size: 7 117 497 831 Fragments arranged by size: 831 497 117 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 351 1452 Size: 351 1101 BglI GCCn_nnn'nGGC Cuts at: 0 215 1027 1180 1438 1452 Size: 215 812 153 258 14 Fragments arranged by size: 812 258 215 153 14 BlpI GC'TnA_GC Cuts at: 0 432 1147 1452 Size: 432 715 305 Fragments arranged by size: 715 432 305 Bme1580I G_kGCm'C Cuts at: 0 1363 1452 Size: 1363 89 BmrI ACTGGGnnnn_n' Cuts at: 0 370 1377 1452 Size: 370 1007 75 Fragments arranged by size: 1007 370 75 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 160 259 271 849 1072 1452 Size: 160 99 12 578 223 380 Fragments arranged by size: 578 380 223 160 99 12 BsaAI yAC'GTr Cuts at: 0 351 1452 Size: 351 1101 BsaHI Gr'CG_yC Cuts at: 0 4 121 618 1452 Size: 4 117 497 834 Fragments arranged by size: 834 497 117 4 BsaWI w'CCGG_w Cuts at: 0 584 1452 Size: 584 868 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 397 427 904 934 1231 1261 1452 Size: 397 30 477 30 297 30 191 Fragments arranged by size: 477 397 297 191 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 268 736 739 846 1452 Size: 268 468 3 107 606 Fragments arranged by size: 606 468 268 107 3 BseYI C'CCAG_C Cuts at: 0 30 65 183 400 884 1355 1452 Size: 30 35 118 217 484 471 97 Fragments arranged by size: 484 471 217 118 97 35 30 BsiEI CG_ry'CG Cuts at: 0 159 201 1452 Size: 159 42 1251 Fragments arranged by size: 1251 159 42 BsiHKAI G_wGCw'C Cuts at: 0 341 654 968 1452 Size: 341 313 314 484 Fragments arranged by size: 484 341 314 313 Bsp1286I G_dGCh'C Cuts at: 0 341 410 433 654 968 1363 1452 Size: 341 69 23 221 314 395 89 Fragments arranged by size: 395 341 314 221 89 69 23 BsrBI CCG'CTC Cuts at: 0 1322 1452 Size: 1322 130 BsrDI GCAATG_nn' Cuts at: 0 912 1452 Size: 912 540 BsrFI r'CCGG_y Cuts at: 0 284 1452 Size: 284 1168 BstEII G'GTnAC_C Cuts at: 0 802 1452 Size: 802 650 BstXI CCAn_nnnn'nTGG Cuts at: 0 798 821 1452 Size: 798 23 631 Fragments arranged by size: 798 631 23 BstYI r'GATC_y Cuts at: 0 516 659 1244 1452 Size: 516 143 585 208 Fragments arranged by size: 585 516 208 143 BtgI C'CryG_G Cuts at: 0 153 275 675 1139 1202 1239 1427 1452 Size: 153 122 400 464 63 37 188 25 Fragments arranged by size: 464 400 188 153 122 63 37 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1381 1452 Size: 1381 71 BtsI GCAGTG_nn' Cuts at: 0 56 1452 Size: 56 1396 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1184 1219 1452 Size: 1184 35 233 Fragments arranged by size: 1184 233 35 DrdI GACnn_nn'nnGTC Cuts at: 0 594 1452 Size: 594 858 EaeI y'GGCC_r Cuts at: 0 53 156 198 1028 1181 1205 1430 1452 Size: 53 103 42 830 153 24 225 22 Fragments arranged by size: 830 225 153 103 53 42 24 22 EagI C'GGCC_G Cuts at: 0 156 198 1452 Size: 156 42 1254 Fragments arranged by size: 1254 156 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 196 1452 Size: 196 1256 EcoICRI GAG'CTC Cuts at: 0 339 966 1452 Size: 339 627 486 Fragments arranged by size: 627 486 339 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 160 259 271 849 1072 1452 Size: 160 99 12 578 223 380 Fragments arranged by size: 578 380 223 160 99 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 526 1452 Size: 526 926 EcoO109I rG'GnC_Cy Cuts at: 0 666 938 1409 1452 Size: 666 272 471 43 Fragments arranged by size: 666 471 272 43 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 435 1452 Size: 435 1017 HaeII r_GCGC'y Cuts at: 0 7 124 621 1452 Size: 7 117 497 831 Fragments arranged by size: 831 497 117 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 397 429 432 464 508 540 1181 1213 Size: 397 32 3 32 44 32 641 32 Cuts at: 1213 1452 Size: 239 Fragments arranged by size: 641 397 239 44 32 32 32 32 3 KasI G'GCGC_C Cuts at: 0 3 120 617 1452 Size: 3 117 497 835 Fragments arranged by size: 835 497 117 3 MscI TGG'CCA Cuts at: 0 1183 1207 1432 1452 Size: 1183 24 225 20 Fragments arranged by size: 1183 225 24 20 MslI CAynn'nnrTG Cuts at: 0 819 1452 Size: 819 633 MspA1I CmG'CkG Cuts at: 0 155 1355 1452 Size: 155 1200 97 Fragments arranged by size: 1200 155 97 NaeI GCC'GGC Cuts at: 0 286 1452 Size: 286 1166 NarI GG'CG_CC Cuts at: 0 4 121 618 1452 Size: 4 117 497 834 Fragments arranged by size: 834 497 117 4 NcoI C'CATG_G Cuts at: 0 275 1427 1452 Size: 275 1152 25 Fragments arranged by size: 1152 275 25 NdeI CA'TA_TG Cuts at: 0 591 1452 Size: 591 861 NgoMIV G'CCGG_C Cuts at: 0 284 1452 Size: 284 1168 NotI GC'GGCC_GC Cuts at: 0 198 1452 Size: 198 1254 NspI r_CATG'y Cuts at: 0 873 1089 1227 1452 Size: 873 216 138 225 Fragments arranged by size: 873 225 216 138 PflMI CCAn_nnn'nTGG Cuts at: 0 330 400 669 1452 Size: 330 70 269 783 Fragments arranged by size: 783 330 269 70 PfoI T'CCnGG_A Cuts at: 0 329 1452 Size: 329 1123 PmlI CAC'GTG Cuts at: 0 351 1452 Size: 351 1101 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 712 744 950 982 1452 Size: 712 32 206 32 470 Fragments arranged by size: 712 470 206 32 32 PpuMI rG'GwC_Cy Cuts at: 0 938 1452 Size: 938 514 PstI C_TGCA'G Cuts at: 0 17 319 1273 1452 Size: 17 302 954 179 Fragments arranged by size: 954 302 179 17 PvuII CAG'CTG Cuts at: 0 1355 1452 Size: 1355 97 SacI G_AGCT'C Cuts at: 0 341 968 1452 Size: 341 627 484 Fragments arranged by size: 627 484 341 SacII CC_GC'GG Cuts at: 0 156 1452 Size: 156 1296 SanDI GG'GwC_CC Cuts at: 0 938 1452 Size: 938 514 SfcI C'TryA_G Cuts at: 0 13 315 1068 1269 1452 Size: 13 302 753 201 183 Fragments arranged by size: 753 302 201 183 13 SfoI GGC'GCC Cuts at: 0 5 122 619 1452 Size: 5 117 497 833 Fragments arranged by size: 833 497 117 5 SmaI CCC'GGG Cuts at: 0 44 1452 Size: 44 1408 SphI G_CATG'C Cuts at: 0 1227 1452 Size: 1227 225 StuI AGG'CCT Cuts at: 0 91 994 1452 Size: 91 903 458 Fragments arranged by size: 903 458 91 StyI C'CwwG_G Cuts at: 0 275 386 1118 1276 1427 1452 Size: 275 111 732 158 151 25 Fragments arranged by size: 732 275 158 151 111 25 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 790 1452 Size: 790 662 TatI w'GTAC_w Cuts at: 0 230 1452 Size: 230 1222 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 335 354 367 375 386 407 644 676 Size: 335 19 13 8 11 21 237 32 Cuts at: 676 904 936 1075 1107 1231 1263 1452 Size: 228 32 139 32 124 32 189 Fragments arranged by size: 335 237 228 189 139 124 32 32 32 32 21 19 13 11 8 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 871 1038 1440 1452 Size: 871 167 402 12 Fragments arranged by size: 871 402 167 12 XmaI C'CCGG_G Cuts at: 0 42 1452 Size: 42 1410 Enzymes that do cut: AccI AflIII AlwNI ApoI AvaI BamHI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmrI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BsrBI BsrDI BsrFI BstEII BstXI BstYI BtgI BtgZI BtsI CspCI DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NspI PflMI PfoI PmlI PpiI PpuMI PstI PvuII SacI SacII SanDI SfcI SfoI SmaI SphI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI BstBI BstZ17I Bsu36I ClaI DraI DraIII EarI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NheI NruI NsiI PacI PasI PciI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI