################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AgeI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FseI FspI HapII HgaI Hin1I HindIII HpaII KasI KpnI Ksp632I KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 610 GTAGAC GT^[AC][GT]AC AlwI 796 GATCC GGATC(4/5) AocI 883 CCTGAGG CC^T[ACGT]AGG Asp700I 998 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 59 CTCGAG C^[CT]CG[GA]G BalI 552 TGGCCA TGG^CCA BfaI 1010 CTAG C^TAG BlpI 1165 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1165 GCTCAGC GC^T[ACGT]AGC BsaAI 343 CACGTA [CT]AC^GT[GA] BseRI 266 GAGGAG GAGGAG(10/8) BsoBI 59 CTCGAG C^[CT]CG[GA]G BsrDI 952 GCAATG GCAATG(2/0) BstBI 125 TTCGAA TT^CGAA BstXI 737 CCAAGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 795 AGATCC [GA]^GATC[CT] Bsu36I 883 CCTGAGG CC^T[ACGT]AGG CelII 1165 GCTCAGC GC^T[ACGT]AGC DsaI 699 CCATGG C^C[GA][CT]GG EaeI 552 TGGCCA [CT]^GGCC[GA] Eco81I 883 CCTGAGG CC^T[ACGT]AGG EcoNI 1096 CCTCTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 811 GATATC GAT^ATC FauI 325 CCCGC CCCGC(4/6) HincII 381 GTTAAC GT[CT]^[GA]AC HindII 381 GTTAAC GT[CT]^[GA]AC HpaI 381 GTTAAC GTT^AAC MaeI 1010 CTAG C^TAG MfeI 367 CAATTG C^AATTG MflI 795 AGATCC [GA]^GATC[CT] MluNI 552 TGGCCA TGG^CCA MscI 552 TGGCCA TGG^CCA MunI 367 CAATTG C^AATTG NcoI 699 CCATGG C^CATGG NspV 125 TTCGAA TT^CGAA PaeR7I 59 CTCGAG C^TCGAG PleI 79 GAGTC GAGTC(4/5) SfaNI 1140 GATGC GCATC(5/9) SfuI 125 TTCGAA TT^CGAA SphI 1013 GCATGC GCATG^C StuI 887 AGGCCT AGG^CCT TfiI 417 GATTC G^A[AT]TC XhoI 59 CTCGAG C^TCGAG XhoII 795 AGATCC [GA]^GATC[CT] XmnI 998 GAATGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 326 CCGC CCGC(-3/-1) 1157 CCGC AcsI 203 AAATTC [GA]^AATT[CT] 258 GAATTT AhdI 307 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 922 GACCACCAGTC ApoI 203 AAATTC [GA]^AATT[CT] 258 GAATTT AspEI 307 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 922 GACCACCAGTC AspHI 499 GAGCAC G[AT]GC[AT]^C 958 GAGCAC BclI 492 TGATCA T^GATCA 717 TGATCA BmyI 499 GAGCAC G[AGT]GC[ACT]^C 958 GAGCAC BsiHKAI 499 GAGCAC G[AT]GC[AT]^C 958 GAGCAC BsiYI 324 CCCCGCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1096 CCTCTGGCAGG BslI 324 CCCCGCCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1096 CCTCTGGCAGG Bsp1286I 499 GAGCAC G[AGT]GC[ACT]^C 958 GAGCAC BspHI 485 TCATGA T^CATGA 495 TCATGA BspLU11I 84 ACATGT A^CATGT 407 ACATGT BstF5I 547 CATCC GGATG(2/0) 1139 GGATG CfoI 1043 GCGC GCG^C 1163 GCGC Eam1105I 307 GACCCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 922 GACCACCAGTC FbaI 492 TGATCA T^GATCA 717 TGATCA FokI 547 CATCC GGATG(9/13) 1139 GGATG HaeII 1042 AGCGCC [GA]GCGC^[CT] 1162 GGCGCT HhaI 1043 GCGC GCG^C 1163 GCGC HinP1I 1043 GCGC G^CGC 1163 GCGC HinfI 79 GAGTC G^A[ACGT]TC 417 GATTC PvuII 118 CAGCTG CAG^CTG 1151 CAGCTG RcaI 485 TCATGA T^CATGA 495 TCATGA SmlI 59 CTCGAG C^T[CT][GA]AG 208 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AfaI AflIII AluI AvaII BbvI BsaJI BsmFI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PpuMI RsaI Sau3AI Sau96I ScrFI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I