Restriction map for pFN21AB6554
 (Circular) (Six-base) MAPSORT of: pFN21AB6554.seq  Check: 4584  from: 1  to: 5878



pFN21AB6554  5878 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:51  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3688     278 
   Size:         53      83     186     936    2152    2468

  Fragments arranged by size: 

                2468    2152     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2616    3221    1937 
   Size:        679     605    4594

  Fragments arranged by size: 

                4594     679     605
 
Acc65I G'GTAC_C

Cuts at:   3199    3199 
   Size:       5878
 
AclI AA'CG_TT

Cuts at:   1563    4005    4378    1563 
   Size:       2442     373    3063

  Fragments arranged by size: 

                3063    2442     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2447    3057    3941    5325    5593    1632 
   Size:        815     610     884    1384     268    1917

  Fragments arranged by size: 

                1917    1384     884     815     610     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5681
 
AflIII A'CryG_T

Cuts at:   1203    2089    2347    2412    4777    5754    1203 
   Size:        886     258      65    2365     977    1327

  Fragments arranged by size: 

                2365    1327     977     886     258      65
 
AgeI A'CCGG_T

Cuts at:   4709    4709 
   Size:       5878
 
AhdI GACnn_n'nnGTC

Cuts at:   2317    2932    4607    2317 
   Size:        615    1675    3588

  Fragments arranged by size: 

                3588    1675     615
 
AleI CACnn'nnGTG

Cuts at:   1166    2984    1166 
   Size:       1818    4060
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5878
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2841    2873    2841 
   Size:         32    5846
 
AlwNI CAG_nnn'CTG

Cuts at:   3621    5193    3621 
   Size:       1572    4306
 
ApaLI G'TGCA_C

Cuts at:   3935    5091    3935 
   Size:       1156    4722
 
ApoI r'AATT_y

Cuts at:   1493    2208    2263    3187    3413    3565    5674    1493 
   Size:        715      55     924     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     924     715     226     152      55
 
AseI AT'TA_AT

Cuts at:    160    1032    4432     160 
   Size:        872    3400    1606

  Fragments arranged by size: 

                3400    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5878
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2064    3203    1870 
   Size:         73     121    1139    4545

  Fragments arranged by size: 

                4545    1139     121      73
 
BamHI G'GATC_C

Cuts at:   3208    3575    3208 
   Size:        367    5511
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3199    4654     618 
   Size:        325     217     627    1412    1455    1842

  Fragments arranged by size: 

                1842    1455    1412     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    3197    1391 
   Size:       1806    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4907
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4069    4103    1903 
   Size:         34    2132      34    3678

  Fragments arranged by size: 

                3678    2132      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3770    4980    3770 
   Size:       1210    4668
 
BclI T'GATC_A

Cuts at:   1548    2497    2722    1548 
   Size:        949     225    4704

  Fragments arranged by size: 

                4704     949     225
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4489     136 
   Size:        107     122      71     631     605    2817    1525

  Fragments arranged by size: 

                2817    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5873    5873 
   Size:       5878
 
BlpI GC'TnA_GC

Cuts at:   3171    3295    3171 
   Size:        124    5754
 
Bme1580I G_kGCm'C

Cuts at:   1790    3939    5095    1790 
   Size:       2149    1156    2573

  Fragments arranged by size: 

                2573    2149    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5878
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2083    2539    3609 
   Size:        416      53      83     399      24     666     456    1070

Cuts at:   3609    4567     442 
   Size:        958    1753

  Fragments arranged by size: 

                1753    1070     958     666     456     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5878
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4538    1239 
   Size:       3299    2579
 
Bpu10I CC'TnA_GC

Cuts at:   2604    2604 
   Size:       5878
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2198    4003    4868    5166    2198 
   Size:       1805     865     298    2910

  Fragments arranged by size: 

                2910    1805     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4541     882 
   Size:        617    3042    2219

  Fragments arranged by size: 

                3042    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2350    3669     493 
   Size:        711    1146    1319    2702

  Fragments arranged by size: 

                2702    1319    1146     711
 
BsaBI GATnn'nnATC

Cuts at:   5872    5872 
   Size:       5878
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3685    4067    4702     275 
   Size:         53      83     186     936    2152     382     635    1451

  Fragments arranged by size: 

                2152    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4311    4709    4983    5130    5541    4311 
   Size:        398     274     147     411    4648

  Fragments arranged by size: 

                4648     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2644    2674    1178 
   Size:         30     165      30    1241      30    4382

  Fragments arranged by size: 

                4382    1241     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2286    1186 
   Size:       1100    4778
 
BseYI C'CCAG_C

Cuts at:   5081    5577    5081 
   Size:        496    5382
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5305
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3328    4089    5117    1540 
   Size:        275     186    1327     761    1028    2301

  Fragments arranged by size: 

                2301    1327    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2508    2967    3939    4024    5095    2508 
   Size:        459     972      85    1071    3291

  Fragments arranged by size: 

                3291    1071     972     459      85
 
BsmI GAATG_Cn'

Cuts at:   3389    3482    3389 
   Size:         93    5785
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2508    2967    3197    3939    4024    5095    1391 
   Size:        399     718     459     230     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     718     459     399     230      85
            
 
BspEI T'CCGG_A

Cuts at:   5541    5541 
   Size:       5878
 
BspHI T'CATG_A

Cuts at:   2490    2500    3767    5497    2490 
   Size:         10    1267    1730    2871

  Fragments arranged by size: 

                2871    1730    1267      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3166    3233     844 
   Size:       2322      67    3489

  Fragments arranged by size: 

                3489    2322      67
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3765    1394 
   Size:        429    1942    3507

  Fragments arranged by size: 

                3507    1942     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2964    4373    4547      65 
   Size:       1183    1716    1409     174    1396

  Fragments arranged by size: 

                1716    1409    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4522    4709    1700 
   Size:        110    2712     187    2869

  Fragments arranged by size: 

                2869    2712     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5878
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5878
 
BssSI C'ACGA_G

Cuts at:   3938    4950    3938 
   Size:       1012    4866
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5878
 
BstBI TT'CG_AA

Cuts at:   2131    4682    2131 
   Size:       2551    3327
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5878
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2749    2749 
   Size:       5878
 
BstYI r'GATC_y

Cuts at:   1972    2800    3208    3575    3960    3977    5429    5515    5527 
   Size:        828     408     367     385      17    1452      86      12

Cuts at:   5527    5873    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     828     408     385     367     346      86
                  17      12
 
Bsu36I CC'TnA_GG

Cuts at:   2889    2889 
   Size:       5878
 
BtgI C'CryG_G

Cuts at:    513    1065    2704     513 
   Size:        552    1639    3687

  Fragments arranged by size: 

                3687    1639     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2857     487 
   Size:        683      56    1631    3508

  Fragments arranged by size: 

                3508    1631     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3394    4188    4208    3394 
   Size:        794      20    5064

  Fragments arranged by size: 

                5064     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5878
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5843
 
DraI TTT'AAA

Cuts at:   3182    3182 
   Size:       5878
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4885     809 
   Size:        507    3569    1802

  Fragments arranged by size: 

                3569    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2557    3325    4214 
   Size:         54    1425      50     275      18     727     768     889

Cuts at:   4214    5843       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     768     727     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3325    1537 
   Size:        275    1513    4090

  Fragments arranged by size: 

                4090    1513     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3808    1353 
   Size:       2455    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4465    4839    4985     387 
   Size:       1064     138    2876     374     146    1280

  Fragments arranged by size: 

                2876    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2447    3057    3941    4538    5325    5593    1239 
   Size:        393     815     610     884     597     787     268    1524

  Fragments arranged by size: 

                1524     884     815     787     610     597     393     268
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3105    3105 
   Size:       5878
 
EcoO109I rG'GnC_Cy

Cuts at:   2012    2276    2431    2600    2012 
   Size:        264     155     169    5290

  Fragments arranged by size: 

                5290     264     169     155
 
EcoRI G'AATT_C

Cuts at:   3187    3565    3187 
   Size:        378    5500
 
EcoRV GAT'ATC

Cuts at:   2818    2818 
   Size:       5878
 
FspI TGC'GCA

Cuts at:   4384    4384 
   Size:       5878
 
HaeII r_GCGC'y

Cuts at:   1678    3051    3171    5025    1678 
   Size:       1373     120    1854    2531

  Fragments arranged by size: 

                2531    1854    1373     120
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2069    2101    2260    2292    2303 
   Size:         32     135      32     497      32     159      32      11

Cuts at:   2303    2335    2918    2950    4519    4551    4593    4625    1373 
   Size:         32     583      32    1569      32      42      32    2626

  Fragments arranged by size: 

                2626    1569     583     497     159     135      42      32
                  32      32      32      32      32      32      32      11
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2388    3222    3468     669 
   Size:        988     281     450     834     246    3079

  Fragments arranged by size: 

                3079     988     834     450     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5878
 
HpaI GTT'AAC

Cuts at:   2388    3468    2388 
   Size:       1080    4798
 
KpnI G_GTAC'C

Cuts at:   3203    3203 
   Size:       5878
 
MfeI C'AATT_G

Cuts at:   2372    3477    2372 
   Size:       1105    4773
 
MluI A'CGCG_T

Cuts at:   5754    5754 
   Size:       5878
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4992    5176    1305 
   Size:       3687     184    2007

  Fragments arranged by size: 

                3687    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2559      10 
   Size:         54    1425     343     727    3329

  Fragments arranged by size: 

                3329    1425     727     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2469    2709    2984    3836    4195    4354     518 
   Size:        648    1303     240     275     852     359     159    2042

  Fragments arranged by size: 

                2042    1303     852     648     359     275     240     159
            
 
MspA1I CmG'CkG

Cuts at:   1606    2125    3158    3164    3294    3971    5119    5364    1606 
   Size:        519    1033       6     130     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1033     677     519     245     130       6
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2704     513 
   Size:        552    1639    3687

  Fragments arranged by size: 

                3687    1639     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5878
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5878
 
NotI GC'GGCC_GC

Cuts at:   3325    3325 
   Size:       5878
 
NspI r_CATG'y

Cuts at:   1132    1229    2093    2416    3022    3236    4781    1132 
   Size:         97     864     323     606     214    1545    2229

  Fragments arranged by size: 

                2229    1545     864     606     323     214      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5878
 
PciI A'CATG_T

Cuts at:   2089    2412    4777    2089 
   Size:        323    2365    3190

  Fragments arranged by size: 

                3190    2365     323
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5878
 
PfoI T'CCnGG_A

Cuts at:   2904    2904 
   Size:       5878
 
PmeI GTTT'AAAC

Cuts at:   3182    3182 
   Size:       5878
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3947    3979    5460    5492    3947 
   Size:         32    1481      32    4333

  Fragments arranged by size: 

                4333    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2012    2276    2600    2012 
   Size:        264     324    5290

  Fragments arranged by size: 

                5290     324     264
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5878
 
PsiI TTA'TAA

Cuts at:   3448    3448 
   Size:       5878
 
PstI C_TGCA'G

Cuts at:    830    3230     830 
   Size:       2400    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5878
 
PvuII CAG'CTG

Cuts at:   2125    3158    2125 
   Size:       1033    4845
 
SalI G'TCGA_C

Cuts at:   1936    3220    1936 
   Size:       1284    4594
 
SanDI GG'GwC_CC

Cuts at:   2012    2012 
   Size:       5878
 
SbfI CC_TGCA'GG

Cuts at:   3230    3230 
   Size:       5878
 
ScaI AGT'ACT

Cuts at:   4126    5838    4126 
   Size:       1712    4166
 
SfcI C'TryA_G

Cuts at:    826    1045    3226    4361    5042    5233     826 
   Size:        219    2181    1135     681     191    1471

  Fragments arranged by size: 

                2181    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5878
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5878
 
SmaI CCC'GGG

Cuts at:   1872    3205    1872 
   Size:       1333    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2064    2213    3982    4883    5145     820 
   Size:        197     926     121     149    1769     901     262    1553

  Fragments arranged by size: 

                1769    1553     926     901     262     197     149     121
            
 
SnaBI TAC'GTA

Cuts at:    493    3669     493 
   Size:       3176    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5878
 
SphI G_CATG'C

Cuts at:   1132    3022    3236    1132 
   Size:       1890     214    3774

  Fragments arranged by size: 

                3774    1890     214
 
SspI AAT'ATT

Cuts at:      5      52    3802       5 
   Size:         47    3750    2081

  Fragments arranged by size: 

                3750    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2894    1517 
   Size:       1377    4501
 
StyI C'CwwG_G

Cuts at:    513    1065    2334    2704    2742    2895    3094    3139    3317 
   Size:        552    1269     370      38     153     199      45     178

Cuts at:   3317     513 
   Size:       3074

  Fragments arranged by size: 

                3074    1269     552     370     199     178     153      45
                  38
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3905    3922    4075    4260    4705     325 
   Size:       1255    2325      17     153     185     445    1498

  Fragments arranged by size: 

                2325    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4124    5836      96 
   Size:        274      80      33      51    1443    2147    1712     138

  Fragments arranged by size: 

                2147    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5838    5870    5838 
   Size:         32    5846
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5878
 
XbaI T'CTAG_A

Cuts at:   3214    3214 
   Size:       5878
 
XhoI C'TCGA_G

Cuts at:   1943    2064    1943 
   Size:        121    5757
 
XmaI C'CCGG_G

Cuts at:   1870    3203    1870 
   Size:       1333    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3007    4007    3007 
   Size:       1000    4878
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3686     276 
   Size:         53      83     186     936    2152    2468

  Fragments arranged by size: 

                2468    2152     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FspI
    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI
     NspI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI
    PshAI     PsiI     PstI     PvuI    PvuII     SalI    SanDI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI    BsiWI    BsmBI  BstZ17I   DraIII  EcoICRI     FalI
     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     NsiI
     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII
     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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