(Circular) (Six-base) MAPSORT of: pFN21AB6554.seq Check: 4584 from: 1 to: 5878 pFN21AB6554 5878 bp With 182 enzymes: SgfI * August 16, 2010 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2616 3221 1937 Size: 679 605 4594 Fragments arranged by size: 4594 679 605 Acc65I G'GTAC_C Cuts at: 3199 3199 Size: 5878 AclI AA'CG_TT Cuts at: 1563 4005 4378 1563 Size: 2442 373 3063 Fragments arranged by size: 3063 2442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2447 3057 3941 5325 5593 1632 Size: 815 610 884 1384 268 1917 Fragments arranged by size: 1917 1384 884 815 610 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 2089 2347 2412 4777 5754 1203 Size: 886 258 65 2365 977 1327 Fragments arranged by size: 2365 1327 977 886 258 65 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 2317 2932 4607 2317 Size: 615 1675 3588 Fragments arranged by size: 3588 1675 615 AleI CACnn'nnGTG Cuts at: 1166 2984 1166 Size: 1818 4060 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5878 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2841 2873 2841 Size: 32 5846 AlwNI CAG_nnn'CTG Cuts at: 3621 5193 3621 Size: 1572 4306 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 2208 2263 3187 3413 3565 5674 1493 Size: 715 55 924 226 152 2109 1697 Fragments arranged by size: 2109 1697 924 715 226 152 55 AseI AT'TA_AT Cuts at: 160 1032 4432 160 Size: 872 3400 1606 Fragments arranged by size: 3400 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 2064 3203 1870 Size: 73 121 1139 4545 Fragments arranged by size: 4545 1139 121 73 BamHI G'GATC_C Cuts at: 3208 3575 3208 Size: 367 5511 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3199 4654 618 Size: 325 217 627 1412 1455 1842 Fragments arranged by size: 1842 1455 1412 627 325 217 BanII G_rGCy'C Cuts at: 1391 3197 1391 Size: 1806 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4907 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4069 4103 1903 Size: 34 2132 34 3678 Fragments arranged by size: 3678 2132 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3770 4980 3770 Size: 1210 4668 BclI T'GATC_A Cuts at: 1548 2497 2722 1548 Size: 949 225 4704 Fragments arranged by size: 4704 949 225 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4489 136 Size: 107 122 71 631 605 2817 1525 Fragments arranged by size: 2817 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5873 5873 Size: 5878 BlpI GC'TnA_GC Cuts at: 3171 3295 3171 Size: 124 5754 Bme1580I G_kGCm'C Cuts at: 1790 3939 5095 1790 Size: 2149 1156 2573 Fragments arranged by size: 2573 2149 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5878 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2083 2539 3609 Size: 416 53 83 399 24 666 456 1070 Cuts at: 3609 4567 442 Size: 958 1753 Fragments arranged by size: 1753 1070 958 666 456 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4538 1239 Size: 3299 2579 Bpu10I CC'TnA_GC Cuts at: 2604 2604 Size: 5878 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2198 4003 4868 5166 2198 Size: 1805 865 298 2910 Fragments arranged by size: 2910 1805 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4541 882 Size: 617 3042 2219 Fragments arranged by size: 3042 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2350 3669 493 Size: 711 1146 1319 2702 Fragments arranged by size: 2702 1319 1146 711 BsaBI GATnn'nnATC Cuts at: 5872 5872 Size: 5878 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3685 4067 4702 275 Size: 53 83 186 936 2152 382 635 1451 Fragments arranged by size: 2152 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4311 4709 4983 5130 5541 4311 Size: 398 274 147 411 4648 Fragments arranged by size: 4648 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2644 2674 1178 Size: 30 165 30 1241 30 4382 Fragments arranged by size: 4382 1241 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2286 1186 Size: 1100 4778 BseYI C'CCAG_C Cuts at: 5081 5577 5081 Size: 496 5382 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5305 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3328 4089 5117 1540 Size: 275 186 1327 761 1028 2301 Fragments arranged by size: 2301 1327 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2508 2967 3939 4024 5095 2508 Size: 459 972 85 1071 3291 Fragments arranged by size: 3291 1071 972 459 85 BsmI GAATG_Cn' Cuts at: 3389 3482 3389 Size: 93 5785 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2508 2967 3197 3939 4024 5095 1391 Size: 399 718 459 230 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 718 459 399 230 85 BspEI T'CCGG_A Cuts at: 5541 5541 Size: 5878 BspHI T'CATG_A Cuts at: 2490 2500 3767 5497 2490 Size: 10 1267 1730 2871 Fragments arranged by size: 2871 1730 1267 10 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3166 3233 844 Size: 2322 67 3489 Fragments arranged by size: 3489 2322 67 BsrBI CCG'CTC Cuts at: 1394 1823 3765 1394 Size: 429 1942 3507 Fragments arranged by size: 3507 1942 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2964 4373 4547 65 Size: 1183 1716 1409 174 1396 Fragments arranged by size: 1716 1409 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4522 4709 1700 Size: 110 2712 187 2869 Fragments arranged by size: 2869 2712 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5878 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 3938 4950 3938 Size: 1012 4866 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5878 BstBI TT'CG_AA Cuts at: 2131 4682 2131 Size: 2551 3327 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstXI CCAn_nnnn'nTGG Cuts at: 2749 2749 Size: 5878 BstYI r'GATC_y Cuts at: 1972 2800 3208 3575 3960 3977 5429 5515 5527 Size: 828 408 367 385 17 1452 86 12 Cuts at: 5527 5873 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 828 408 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2889 2889 Size: 5878 BtgI C'CryG_G Cuts at: 513 1065 2704 513 Size: 552 1639 3687 Fragments arranged by size: 3687 1639 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2857 487 Size: 683 56 1631 3508 Fragments arranged by size: 3508 1631 683 56 BtsI GCAGTG_nn' Cuts at: 3394 4188 4208 3394 Size: 794 20 5064 Fragments arranged by size: 5064 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5843 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4885 809 Size: 507 3569 1802 Fragments arranged by size: 3569 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2557 3325 4214 Size: 54 1425 50 275 18 727 768 889 Cuts at: 4214 5843 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 768 727 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3325 1537 Size: 275 1513 4090 Fragments arranged by size: 4090 1513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3808 1353 Size: 2455 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4465 4839 4985 387 Size: 1064 138 2876 374 146 1280 Fragments arranged by size: 2876 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2447 3057 3941 4538 5325 5593 1239 Size: 393 815 610 884 597 787 268 1524 Fragments arranged by size: 1524 884 815 787 610 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3105 3105 Size: 5878 EcoO109I rG'GnC_Cy Cuts at: 2012 2276 2431 2600 2012 Size: 264 155 169 5290 Fragments arranged by size: 5290 264 169 155 EcoRI G'AATT_C Cuts at: 3187 3565 3187 Size: 378 5500 EcoRV GAT'ATC Cuts at: 2818 2818 Size: 5878 FspI TGC'GCA Cuts at: 4384 4384 Size: 5878 HaeII r_GCGC'y Cuts at: 1678 3051 3171 5025 1678 Size: 1373 120 1854 2531 Fragments arranged by size: 2531 1854 1373 120 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2069 2101 2260 2292 2303 Size: 32 135 32 497 32 159 32 11 Cuts at: 2303 2335 2918 2950 4519 4551 4593 4625 1373 Size: 32 583 32 1569 32 42 32 2626 Fragments arranged by size: 2626 1569 583 497 159 135 42 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 2388 3222 3468 669 Size: 988 281 450 834 246 3079 Fragments arranged by size: 3079 988 834 450 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5878 HpaI GTT'AAC Cuts at: 2388 3468 2388 Size: 1080 4798 KpnI G_GTAC'C Cuts at: 3203 3203 Size: 5878 MfeI C'AATT_G Cuts at: 2372 3477 2372 Size: 1105 4773 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4992 5176 1305 Size: 3687 184 2007 Fragments arranged by size: 3687 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2559 10 Size: 54 1425 343 727 3329 Fragments arranged by size: 3329 1425 727 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2469 2709 2984 3836 4195 4354 518 Size: 648 1303 240 275 852 359 159 2042 Fragments arranged by size: 2042 1303 852 648 359 275 240 159 MspA1I CmG'CkG Cuts at: 1606 2125 3158 3164 3294 3971 5119 5364 1606 Size: 519 1033 6 130 677 1148 245 2120 Fragments arranged by size: 2120 1148 1033 677 519 245 130 6 NcoI C'CATG_G Cuts at: 513 1065 2704 513 Size: 552 1639 3687 Fragments arranged by size: 3687 1639 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5878 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 2093 2416 3022 3236 4781 1132 Size: 97 864 323 606 214 1545 2229 Fragments arranged by size: 2229 1545 864 606 323 214 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5878 PciI A'CATG_T Cuts at: 2089 2412 4777 2089 Size: 323 2365 3190 Fragments arranged by size: 3190 2365 323 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5878 PfoI T'CCnGG_A Cuts at: 2904 2904 Size: 5878 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3947 3979 5460 5492 3947 Size: 32 1481 32 4333 Fragments arranged by size: 4333 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2012 2276 2600 2012 Size: 264 324 5290 Fragments arranged by size: 5290 324 264 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5878 PsiI TTA'TAA Cuts at: 3448 3448 Size: 5878 PstI C_TGCA'G Cuts at: 830 3230 830 Size: 2400 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 PvuII CAG'CTG Cuts at: 2125 3158 2125 Size: 1033 4845 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SanDI GG'GwC_CC Cuts at: 2012 2012 Size: 5878 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 4126 5838 4126 Size: 1712 4166 SfcI C'TryA_G Cuts at: 826 1045 3226 4361 5042 5233 826 Size: 219 2181 1135 681 191 1471 Fragments arranged by size: 2181 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5878 SmaI CCC'GGG Cuts at: 1872 3205 1872 Size: 1333 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2064 2213 3982 4883 5145 820 Size: 197 926 121 149 1769 901 262 1553 Fragments arranged by size: 1769 1553 926 901 262 197 149 121 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 3022 3236 1132 Size: 1890 214 3774 Fragments arranged by size: 3774 1890 214 SspI AAT'ATT Cuts at: 5 52 3802 5 Size: 47 3750 2081 Fragments arranged by size: 3750 2081 47 StuI AGG'CCT Cuts at: 1517 2894 1517 Size: 1377 4501 StyI C'CwwG_G Cuts at: 513 1065 2334 2704 2742 2895 3094 3139 3317 Size: 552 1269 370 38 153 199 45 178 Cuts at: 3317 513 Size: 3074 Fragments arranged by size: 3074 1269 552 370 199 178 153 45 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3905 3922 4075 4260 4705 325 Size: 1255 2325 17 153 185 445 1498 Fragments arranged by size: 2325 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4124 5836 96 Size: 274 80 33 51 1443 2147 1712 138 Fragments arranged by size: 2147 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5838 5870 5838 Size: 32 5846 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5878 XbaI T'CTAG_A Cuts at: 3214 3214 Size: 5878 XhoI C'TCGA_G Cuts at: 1943 2064 1943 Size: 121 5757 XmaI C'CCGG_G Cuts at: 1870 3203 1870 Size: 1333 4545 XmnI GAAnn'nnTTC Cuts at: 3007 4007 3007 Size: 1000 4878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI