Restriction map for
pFN21AB6554
(Linear) (Six-base) MAPSORT of: KIBB6554.seq Check: 613 from: 1 to: 1173
KIBB6554.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AccI GT'mk_AC
Cuts at: 0 611 1173
Size: 611 562
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 1052 1173
Size: 442 610 121
Fragments arranged by size:
610 442 121
AflIII A'CryG_T
Cuts at: 0 84 342 407 1173
Size: 84 258 65 766
Fragments arranged by size:
766 258 84 65
AhdI GACnn_n'nnGTC
Cuts at: 0 312 927 1173
Size: 312 615 246
Fragments arranged by size:
615 312 246
AleI CACnn'nnGTG
Cuts at: 0 979 1173
Size: 979 194
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 836 868 1173
Size: 836 32 305
Fragments arranged by size:
836 305 32
ApoI r'AATT_y
Cuts at: 0 203 258 1173
Size: 203 55 915
Fragments arranged by size:
915 203 55
AvaI C'yCGr_G
Cuts at: 0 59 1173
Size: 59 1114
BclI T'GATC_A
Cuts at: 0 492 717 1173
Size: 492 225 456
Fragments arranged by size:
492 456 225
BlpI GC'TnA_GC
Cuts at: 0 1166 1173
Size: 1166 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 78 534 1173
Size: 78 456 639
Fragments arranged by size:
639 456 78
Bpu10I CC'TnA_GC
Cuts at: 0 599 1173
Size: 599 574
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 1173
Size: 193 980
BsaAI yAC'GTr
Cuts at: 0 345 1173
Size: 345 828
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 639 669 1173
Size: 639 30 504
Fragments arranged by size:
639 504 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 281 1173
Size: 281 892
BsiHKAI G_wGCw'C
Cuts at: 0 503 962 1173
Size: 503 459 211
Fragments arranged by size:
503 459 211
Bsp1286I G_dGCh'C
Cuts at: 0 503 962 1173
Size: 503 459 211
Fragments arranged by size:
503 459 211
BspHI T'CATG_A
Cuts at: 0 485 495 1173
Size: 485 10 678
Fragments arranged by size:
678 485 10
BsrDI GCAATG_nn'
Cuts at: 0 959 1173
Size: 959 214
BstBI TT'CG_AA
Cuts at: 0 126 1173
Size: 126 1047
BstXI CCAn_nnnn'nTGG
Cuts at: 0 744 1173
Size: 744 429
BstYI r'GATC_y
Cuts at: 0 795 1173
Size: 795 378
Bsu36I CC'TnA_GG
Cuts at: 0 884 1173
Size: 884 289
BtgI C'CryG_G
Cuts at: 0 699 1173
Size: 699 474
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 852 1173
Size: 852 321
EaeI y'GGCC_r
Cuts at: 0 552 1173
Size: 552 621
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 1052 1173
Size: 442 610 121
Fragments arranged by size:
610 442 121
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1100 1173
Size: 1100 73
EcoO109I rG'GnC_Cy
Cuts at: 0 7 271 426 595 1173
Size: 7 264 155 169 578
Fragments arranged by size:
578 264 169 155 7
EcoRV GAT'ATC
Cuts at: 0 813 1173
Size: 813 360
HaeII r_GCGC'y
Cuts at: 0 1046 1166 1173
Size: 1046 120 7
Fragments arranged by size:
1046 120 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 64 96 255 287 298 330 913 945
Size: 64 32 159 32 11 32 583 32
Cuts at: 945 1173
Size: 228
Fragments arranged by size:
583 228 159 64 32 32 32 32
11
HincII GTy'rAC
Cuts at: 0 383 1173
Size: 383 790
HpaI GTT'AAC
Cuts at: 0 383 1173
Size: 383 790
MfeI C'AATT_G
Cuts at: 0 367 1173
Size: 367 806
MscI TGG'CCA
Cuts at: 0 554 1173
Size: 554 619
MslI CAynn'nnrTG
Cuts at: 0 464 704 979 1173
Size: 464 240 275 194
Fragments arranged by size:
464 275 240 194
MspA1I CmG'CkG
Cuts at: 0 120 1153 1159 1173
Size: 120 1033 6 14
Fragments arranged by size:
1033 120 14 6
NcoI C'CATG_G
Cuts at: 0 699 1173
Size: 699 474
NspI r_CATG'y
Cuts at: 0 88 411 1017 1173
Size: 88 323 606 156
Fragments arranged by size:
606 323 156 88
PciI A'CATG_T
Cuts at: 0 84 407 1173
Size: 84 323 766
Fragments arranged by size:
766 323 84
PfoI T'CCnGG_A
Cuts at: 0 899 1173
Size: 899 274
PpuMI rG'GwC_Cy
Cuts at: 0 7 271 595 1173
Size: 7 264 324 578
Fragments arranged by size:
578 324 264 7
PvuII CAG'CTG
Cuts at: 0 120 1153 1173
Size: 120 1033 20
Fragments arranged by size:
1033 120 20
SanDI GG'GwC_CC
Cuts at: 0 7 1173
Size: 7 1166
SmlI C'TyrA_G
Cuts at: 0 59 208 1173
Size: 59 149 965
Fragments arranged by size:
965 149 59
SphI G_CATG'C
Cuts at: 0 1017 1173
Size: 1017 156
StuI AGG'CCT
Cuts at: 0 889 1173
Size: 889 284
StyI C'CwwG_G
Cuts at: 0 329 699 737 890 1089 1134 1173
Size: 329 370 38 153 199 45 39
Fragments arranged by size:
370 329 199 153 45 39 38
XhoI C'TCGA_G
Cuts at: 0 59 1173
Size: 59 1114
XmnI GAAnn'nnTTC
Cuts at: 0 1002 1173
Size: 1002 171
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI AloI ApoI AvaI
BclI BlpI BmrI Bpu10I BpuEI BsaAI BsaXI BseRI
BsiHKAI Bsp1286I BspHI BsrDI BstBI BstXI BstYI Bsu36I
BtgI BtgZI EaeI Eco57MI EcoNI EcoO109I EcoRV HaeII
Hin4I HincII HpaI MfeI MscI MslI MspA1I NcoI
NspI PciI PfoI PpuMI PvuII SanDI SmlI SphI
StuI StyI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AlfI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BglII Bme1580I BmgBI BmtI BplI BpmI
BsaI BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstEII BstZ17I BtsI ClaI CspCI DraI
DraIII DrdI EagI EarI EciI EcoICRI EcoRI FalI
FseI FspI FspAI HindIII KasI KpnI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI