(Linear) (Six-base) MAPSORT of: KIEE2507.seq Check: 565 from: 1 to: 1320 KIEE2507.orf With 182 enzymes: SgfI * February 14, 2014 11:03 .. Acc65I G'GTAC_C Cuts at: 0 1139 1320 Size: 1139 181 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 856 873 931 1060 1320 Size: 154 702 17 58 129 260 Fragments arranged by size: 702 260 154 129 58 17 AhdI GACnn_n'nnGTC Cuts at: 0 168 321 1320 Size: 168 153 999 Fragments arranged by size: 999 168 153 AleI CACnn'nnGTG Cuts at: 0 1307 1320 Size: 1307 13 AlwNI CAG_nnn'CTG Cuts at: 0 447 675 692 834 1186 1320 Size: 447 228 17 142 352 134 Fragments arranged by size: 447 352 228 142 134 17 ApaI G_GGCC'C Cuts at: 0 68 219 907 1320 Size: 68 151 688 413 Fragments arranged by size: 688 413 151 68 ApaLI G'TGCA_C Cuts at: 0 871 1108 1320 Size: 871 237 212 Fragments arranged by size: 871 237 212 AvaI C'yCGr_G Cuts at: 0 794 1285 1320 Size: 794 491 35 Fragments arranged by size: 794 491 35 BamHI G'GATC_C Cuts at: 0 512 848 1025 1320 Size: 512 336 177 295 Fragments arranged by size: 512 336 295 177 BanI G'GyrC_C Cuts at: 0 1139 1320 Size: 1139 181 BanII G_rGCy'C Cuts at: 0 68 219 907 1121 1320 Size: 68 151 688 214 199 Fragments arranged by size: 688 214 199 151 68 BglI GCCn_nnn'nGGC Cuts at: 0 151 1320 Size: 151 1169 Bme1580I G_kGCm'C Cuts at: 0 68 219 362 460 875 907 1112 1320 Size: 68 151 143 98 415 32 205 208 Fragments arranged by size: 415 208 205 151 143 98 68 32 BmgBI CAC'GTC Cuts at: 0 141 1320 Size: 141 1179 BmrI ACTGGGnnnn_n' Cuts at: 0 1092 1320 Size: 1092 228 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 277 1320 Size: 277 1043 Bpu10I CC'TnA_GC Cuts at: 0 1115 1320 Size: 1115 205 BsaI GGTCTCn'nnnn_ Cuts at: 0 517 1292 1320 Size: 517 775 28 Fragments arranged by size: 775 517 28 BsaBI GATnn'nnATC Cuts at: 0 127 1320 Size: 127 1193 BsaHI Gr'CG_yC Cuts at: 0 41 367 496 1320 Size: 41 326 129 824 Fragments arranged by size: 824 326 129 41 BsaWI w'CCGG_w Cuts at: 0 287 1320 Size: 287 1033 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 391 1000 1320 Size: 391 609 320 Fragments arranged by size: 609 391 320 BseYI C'CCAG_C Cuts at: 0 642 1179 1204 1320 Size: 642 537 25 116 Fragments arranged by size: 642 537 116 25 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 55 96 421 688 918 1320 Size: 55 41 325 267 230 402 Fragments arranged by size: 402 325 267 230 55 41 BsiEI CG_ry'CG Cuts at: 0 329 386 1320 Size: 329 57 934 Fragments arranged by size: 934 329 57 BsiHKAI G_wGCw'C Cuts at: 0 602 725 875 1112 1121 1320 Size: 602 123 150 237 9 199 Fragments arranged by size: 602 237 199 150 123 9 BsmBI CGTCTCn'nnnn_ Cuts at: 0 373 1320 Size: 373 947 Bsp1286I G_dGCh'C Cuts at: 0 68 219 362 460 602 725 875 907 Size: 68 151 143 98 142 123 150 32 Cuts at: 907 1112 1121 1320 Size: 205 9 199 Fragments arranged by size: 205 199 151 150 143 142 123 98 68 32 9 BspEI T'CCGG_A Cuts at: 0 287 1320 Size: 287 1033 BspHI T'CATG_A Cuts at: 0 56 1320 Size: 56 1264 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 683 1320 Size: 683 637 BsrBI CCG'CTC Cuts at: 0 636 1320 Size: 636 684 BsrDI GCAATG_nn' Cuts at: 0 91 341 1320 Size: 91 250 979 Fragments arranged by size: 979 250 91 BsrFI r'CCGG_y Cuts at: 0 419 1320 Size: 419 901 BssSI C'ACGA_G Cuts at: 0 461 874 1320 Size: 461 413 446 Fragments arranged by size: 461 446 413 BstAPI GCAn_nnn'nTGC Cuts at: 0 675 1320 Size: 675 645 BstYI r'GATC_y Cuts at: 0 182 482 512 848 1001 1025 1320 Size: 182 300 30 336 153 24 295 Fragments arranged by size: 336 300 295 182 153 30 24 BtgI C'CryG_G Cuts at: 0 76 362 516 852 1224 1320 Size: 76 286 154 336 372 96 Fragments arranged by size: 372 336 286 154 96 76 BtsI GCAGTG_nn' Cuts at: 0 303 569 705 1320 Size: 303 266 136 615 Fragments arranged by size: 615 303 266 136 DrdI GACnn_nn'nnGTC Cuts at: 0 751 1102 1320 Size: 751 351 218 Fragments arranged by size: 751 351 218 EaeI y'GGCC_r Cuts at: 0 102 143 326 383 1269 1320 Size: 102 41 183 57 886 51 Fragments arranged by size: 886 183 102 57 51 41 EagI C'GGCC_G Cuts at: 0 326 383 1320 Size: 326 57 937 Fragments arranged by size: 937 326 57 EarI CTCTTCn'nnn_ Cuts at: 0 607 685 1320 Size: 607 78 635 Fragments arranged by size: 635 607 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1306 1320 Size: 1306 14 EcoICRI GAG'CTC Cuts at: 0 1119 1320 Size: 1119 201 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 277 856 873 931 1060 1320 Size: 154 123 579 17 58 129 260 Fragments arranged by size: 579 260 154 129 123 58 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 202 838 1320 Size: 202 636 482 Fragments arranged by size: 636 482 202 EcoO109I rG'GnC_Cy Cuts at: 0 27 65 195 215 216 252 672 773 Size: 27 38 130 20 1 36 420 101 Cuts at: 773 831 903 904 1320 Size: 58 72 1 416 Fragments arranged by size: 420 416 130 101 72 58 38 36 27 20 1 1 HaeII r_GCGC'y Cuts at: 0 448 506 571 643 823 1211 1320 Size: 448 58 65 72 180 388 109 Fragments arranged by size: 448 388 180 109 72 65 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 38 70 154 186 307 339 481 513 Size: 38 32 84 32 121 32 142 32 Cuts at: 513 952 984 1320 Size: 439 32 336 Fragments arranged by size: 439 336 142 121 84 38 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1053 1320 Size: 1053 267 KpnI G_GTAC'C Cuts at: 0 1143 1320 Size: 1143 177 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 69 602 860 1320 Size: 69 533 258 460 Fragments arranged by size: 533 460 258 69 MscI TGG'CCA Cuts at: 0 145 1271 1320 Size: 145 1126 49 Fragments arranged by size: 1126 145 49 MslI CAynn'nnrTG Cuts at: 0 976 1307 1320 Size: 976 331 13 Fragments arranged by size: 976 331 13 MspA1I CmG'CkG Cuts at: 0 78 364 518 854 1183 1320 Size: 78 286 154 336 329 137 Fragments arranged by size: 336 329 286 154 137 78 PflMI CCAn_nnn'nTGG Cuts at: 0 1082 1320 Size: 1082 238 PpuMI rG'GwC_Cy Cuts at: 0 195 252 672 1320 Size: 195 57 420 648 Fragments arranged by size: 648 420 195 57 PspOMI G'GGCC_C Cuts at: 0 64 215 903 1320 Size: 64 151 688 417 Fragments arranged by size: 688 417 151 64 PstI C_TGCA'G Cuts at: 0 653 671 1320 Size: 653 18 649 Fragments arranged by size: 653 649 18 PvuII CAG'CTG Cuts at: 0 1183 1320 Size: 1183 137 SacI G_AGCT'C Cuts at: 0 1121 1320 Size: 1121 199 SacII CC_GC'GG Cuts at: 0 79 365 519 855 1320 Size: 79 286 154 336 465 Fragments arranged by size: 465 336 286 154 79 SapI GCTCTTCn'nnn_ Cuts at: 0 607 685 1320 Size: 607 78 635 Fragments arranged by size: 635 607 78 SbfI CC_TGCA'GG Cuts at: 0 671 1320 Size: 671 649 SfcI C'TryA_G Cuts at: 0 649 667 1134 1320 Size: 649 18 467 186 Fragments arranged by size: 649 467 186 18 SfiI GGCCn_nnn'nGGCC Cuts at: 0 151 1320 Size: 151 1169 SmaI CCC'GGG Cuts at: 0 796 1320 Size: 796 524 StuI AGG'CCT Cuts at: 0 583 1320 Size: 583 737 StyI C'CwwG_G Cuts at: 0 527 578 1320 Size: 527 51 742 Fragments arranged by size: 742 527 51 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 936 1093 1320 Size: 936 157 227 Fragments arranged by size: 936 227 157 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 282 314 986 1018 1320 Size: 282 32 672 32 302 Fragments arranged by size: 672 302 282 32 32 Tth111I GACn'n_nGTC Cuts at: 0 545 1320 Size: 545 775 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 252 1320 Size: 252 1068 XmaI C'CCGG_G Cuts at: 0 794 1320 Size: 794 526 Enzymes that do cut: Acc65I AcuI AhdI AleI AlwNI ApaI ApaLI AvaI BamHI BanI BanII BglI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstYI BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I PflMI PpuMI PspOMI PstI PvuII SacI SacII SapI SbfI SfcI SfiI SmaI StuI StyI TaqII TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI BplI BpuEI BsaAI BsaXI BsiWI BsmI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI ScaI SexAI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI XhoI XmnI ZraI