Restriction map for
pFN21AE3933
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AlwI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BseAI BseRI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssSI Bst1107I BstBI BstEII BstF5I
BstPI BstYI BstZ17I Bsu36I CelII
ClaI DpnII DraI DrdI Eam1105I
EarI Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I MaeIII MamI MboI
MfeI MflI MluI MroI MseI
MunI NaeI NcoI NdeI NdeII
NgoMIV NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsaI SalI SapI Sau3AI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tsp509I Tth111I
Van91I XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcyI 37 GGCGCC G[GA]^CG[CT]C
Aor51HI 51 AGCGCT AGC^GCT
BalI 296 TGGCCA TGG^CCA
BbeI 37 GGCGCC GGCGC^C
BbvCI 540 GCTGAGG CCTCAGC(-5/-2)
BpmI 562 CTGGAG CTGGAG(16/14)
BsaHI 37 GGCGCC G[GA]^CG[CT]C
BsaI 350 GGTCTC GGTCTC(1/5)
BsaWI 581 TCCGGT [AT]^CCGG[AT]
BsgI 508 GTGCAG GTGCAG(16/14)
BsmAI 351 GTCTC GTCTC(1/5)
BstXI 440 CCATCTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 19 CGGACCG CG^G[AT]CCG
DraII 636 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 481 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 359 CGGCCG C^GGCCG
EclXI 359 CGGCCG C^GGCCG
Eco47III 51 AGCGCT AGC^GCT
Eco52I 359 CGGCCG C^GGCCG
EcoO109I 636 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 518 CCAAGG C^C[AT][AT]GG
HgaI 434 GACGC GACGC(5/10)
Hin1I 37 GGCGCC G[GA]^CG[CT]C
HinfI 532 GAGTC G^A[ACGT]TC
HphI 618 GGTGA GGTGA(8/7)
KasI 37 GGCGCC G^GCGCC
MaeII 82 ACGT A^CGT
MboII 552 GAAGA GAAGA(8/7)
MluNI 296 TGGCCA TGG^CCA
MscI 296 TGGCCA TGG^CCA
MslI 441 CATCTGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 37 GGCGCC GG^CGCC
NlaIII 397 CATG CATG^
NotI 358 GCGGCCGC GC^GGCCGC
PleI 532 GAGTC GAGTC(4/5)
PstI 67 CTGCAG CTGCA^G
PvuII 265 CAGCTG CAG^CTG
RsrII 19 CGGACCG CG^G[AT]CCG
SfcI 67 CTGCAG C^T[GA][CT]AG
SfoI 37 GGCGCC GGC^GCC
StyI 518 CCAAGG C^C[AT][AT]GG
TaqI 125 TCGA T^CGA
TthHB8I 125 TCGA T^CGA
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwNI 138 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
374 CAGCGGCTG
BanI 37 GGCGCC G^G[CT][GA]CC
627 GGCACC
BfaI 49 CTAG C^TAG
97 CTAG
BsiEI 359 CGGCCG CG[GA][CT]^CG
456 CGACCG
BsmFI 12 GGGAC GGGAC(10/14)
647 GGGAC
BsrI 561 ACTGG ACTGG(1/-1)
260 CCAGT
BssHII 209 GCGCGC G^CGCGC
253 GCGCGC
BstAPI 373 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
480 GCACGCCGTGC
DdeI 353 CTCAG C^T[ACGT]AG
541 CTGAG
MaeI 49 CTAG C^TAG
97 CTAG
SacI 100 GAGCTC GAGCT^C
523 GAGCTC
TspRI 224 CACTG CA[GC]TG[ACGT][ACGT]^
238 CAGTG
XcmI 275 CCAACCGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
518 CCAAGGAGCTCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BsiHKAI BsiYI BslI BsoBI
Bsp1286I BsrFI BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DsaI
EaeI EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
ItaI KspI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NlaIV
SacII Sau96I ScrFI SmaI TseI
XmaCI XmaI