(Circular) (Six-base) MAPSORT of: pFN21AE3933.seq Check: 7694 from: 1 to: 5368 pFN21AE3933 5368 bp With 182 enzymes: SgfI * March 28, 2012 11:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3178 278 Size: 53 83 186 936 1642 2468 Fragments arranged by size: 2468 1642 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2711 1937 Size: 774 4594 Acc65I G'GTAC_C Cuts at: 2689 2689 Size: 5368 AclI AA'CG_TT Cuts at: 1563 3495 3868 1563 Size: 1932 373 3063 Fragments arranged by size: 3063 1932 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2305 3431 4815 5083 1632 Size: 673 1126 1384 268 1917 Fragments arranged by size: 1917 1384 1126 673 268 AfeI AGC'GCT Cuts at: 2058 2058 Size: 5368 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5171 AflIII A'CryG_T Cuts at: 1203 4267 5244 1203 Size: 3064 977 1327 Fragments arranged by size: 3064 1327 977 AgeI A'CCGG_T Cuts at: 4199 4199 Size: 5368 AhdI GACnn_n'nnGTC Cuts at: 4097 4097 Size: 5368 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5368 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2633 1184 Size: 1449 3919 AlwNI CAG_nnn'CTG Cuts at: 2148 2384 3111 4683 2148 Size: 236 727 1572 2833 Fragments arranged by size: 2833 1572 727 236 ApaLI G'TGCA_C Cuts at: 3425 4581 3425 Size: 1156 4212 ApoI r'AATT_y Cuts at: 1493 2677 2903 3055 5164 1493 Size: 1184 226 152 2109 1697 Fragments arranged by size: 2109 1697 1184 226 152 AseI AT'TA_AT Cuts at: 160 1032 3922 160 Size: 872 2890 1606 Fragments arranged by size: 2890 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5368 AvaI C'yCGr_G Cuts at: 1870 1943 2182 2341 2373 2693 1870 Size: 73 239 159 32 320 4545 Fragments arranged by size: 4545 320 239 159 73 32 BamHI G'GATC_C Cuts at: 2698 3065 2698 Size: 367 5001 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2632 2689 4144 618 Size: 325 217 627 255 590 57 1455 1842 Fragments arranged by size: 1842 1455 627 590 325 255 217 57 BanII G_rGCy'C Cuts at: 1391 2109 2256 2532 2687 1391 Size: 718 147 276 155 4072 Fragments arranged by size: 4072 718 276 155 147 BbeI G_GCGC'C Cuts at: 2046 2046 Size: 5368 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4397 BbvCI CC'TCA_GC Cuts at: 2546 2546 Size: 5368 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3559 3593 1903 Size: 34 1622 34 3678 Fragments arranged by size: 3678 1622 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3260 4470 3260 Size: 1210 4158 BclI T'GATC_A Cuts at: 1548 1548 Size: 5368 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3979 136 Size: 107 122 71 631 605 337 1970 1525 Fragments arranged by size: 1970 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5363 5363 Size: 5368 BlpI GC'TnA_GC Cuts at: 2785 2785 Size: 5368 Bme1580I G_kGCm'C Cuts at: 1790 3429 4585 1790 Size: 1639 1156 2573 Fragments arranged by size: 2573 1639 1156 BmgBI CAC'GTC Cuts at: 1318 2088 1318 Size: 770 4598 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2259 3099 4057 Size: 416 53 83 399 24 842 840 958 Cuts at: 4057 442 Size: 1753 Fragments arranged by size: 1753 958 842 840 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5368 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2588 4028 1239 Size: 1349 1440 2579 Fragments arranged by size: 2579 1440 1349 Bpu10I CC'TnA_GC Cuts at: 2546 2546 Size: 5368 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3493 4358 4656 3493 Size: 865 298 4205 Fragments arranged by size: 4205 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2361 4031 882 Size: 617 862 1670 2219 Fragments arranged by size: 2219 1670 862 617 BsaAI yAC'GTr Cuts at: 493 1204 3159 493 Size: 711 1955 2702 Fragments arranged by size: 2702 1955 711 BsaBI GATnn'nnATC Cuts at: 5362 5362 Size: 5368 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 3175 3557 4192 Size: 53 83 186 936 510 1132 382 635 Cuts at: 4192 275 Size: 1451 Fragments arranged by size: 1451 1132 936 635 510 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2586 3801 4199 4473 4620 5031 2586 Size: 1215 398 274 147 411 2923 Fragments arranged by size: 2923 1215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5143 Fragments arranged by size: 5143 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5368 BseYI C'CCAG_C Cuts at: 2172 4571 5067 2172 Size: 2399 496 2473 Fragments arranged by size: 2473 2399 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2534 1163 Size: 573 798 3997 Fragments arranged by size: 3997 798 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2367 2464 2818 3579 4607 1540 Size: 275 186 366 97 354 761 1028 2301 Fragments arranged by size: 2301 1028 761 366 354 275 186 97 BsiHKAI G_wGCw'C Cuts at: 2093 2109 2352 2478 2487 2532 3429 3514 4585 Size: 16 243 126 9 45 897 85 1071 Cuts at: 4585 2093 Size: 2876 Fragments arranged by size: 2876 1071 897 243 126 85 45 16 9 BsmI GAATG_Cn' Cuts at: 2879 2972 2879 Size: 93 5275 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2109 2256 2352 2478 2487 2532 Size: 399 303 16 147 96 126 9 45 Cuts at: 2532 2687 3429 3514 4585 1391 Size: 155 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 303 155 147 126 96 85 45 16 9 BspEI T'CCGG_A Cuts at: 5031 5031 Size: 5368 BspHI T'CATG_A Cuts at: 3257 4987 3257 Size: 1730 3638 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2723 844 Size: 1879 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3255 1394 Size: 429 1432 3507 Fragments arranged by size: 3507 1432 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3863 4037 65 Size: 1183 2615 174 1396 Fragments arranged by size: 2615 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2027 2039 2119 4012 4199 1700 Size: 110 217 12 80 1893 187 2869 Fragments arranged by size: 2869 1893 217 187 110 80 12 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5368 BssHII G'CGCG_C Cuts at: 1925 2214 2258 1925 Size: 289 44 5035 Fragments arranged by size: 5035 289 44 BssSI C'ACGA_G Cuts at: 3428 4440 3428 Size: 1012 4356 BstAPI GCAn_nnn'nTGC Cuts at: 794 2384 2491 794 Size: 1590 107 3671 Fragments arranged by size: 3671 1590 107 BstBI TT'CG_AA Cuts at: 4172 4172 Size: 5368 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5368 BstXI CCAn_nnnn'nTGG Cuts at: 2452 2452 Size: 5368 BstYI r'GATC_y Cuts at: 1972 2698 3065 3450 3467 4919 5005 5017 5363 Size: 726 367 385 17 1452 86 12 346 Cuts at: 5363 1972 Size: 1977 Fragments arranged by size: 1977 1452 726 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2021 2331 2367 2592 2648 513 Size: 552 939 17 310 36 225 56 3233 Fragments arranged by size: 3233 939 552 310 225 56 36 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4629 Fragments arranged by size: 4629 683 56 BtsI GCAGTG_nn' Cuts at: 2228 2249 2884 3678 3698 2228 Size: 21 635 794 20 3898 Fragments arranged by size: 3898 794 635 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5368 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5333 DraI TTT'AAA Cuts at: 2672 2672 Size: 5368 DraIII CAC_nnn'GTG Cuts at: 2491 2491 Size: 5368 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4375 809 Size: 507 3059 1802 Fragments arranged by size: 3059 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 2301 2364 Size: 54 1425 50 275 18 307 164 63 Cuts at: 2364 2815 3704 5333 8 Size: 451 889 1629 43 Fragments arranged by size: 1629 1425 889 451 307 275 164 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2364 2815 1537 Size: 275 552 451 4090 Fragments arranged by size: 4090 552 451 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3298 1353 Size: 1945 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3955 4329 4475 387 Size: 1064 138 2366 374 146 1280 Fragments arranged by size: 2366 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2107 2530 2107 Size: 423 4945 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2305 2588 3431 4028 4815 5083 1239 Size: 393 673 283 843 597 787 268 1524 Fragments arranged by size: 1524 843 787 673 597 393 283 268 EcoO109I rG'GnC_Cy Cuts at: 2642 2642 Size: 5368 EcoRI G'AATT_C Cuts at: 2677 3055 2677 Size: 378 4990 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2548 2548 Size: 5368 FspI TGC'GCA Cuts at: 3874 3874 Size: 5368 HaeII r_GCGC'y Cuts at: 1678 2046 2060 2170 2182 2320 4515 1678 Size: 368 14 110 12 138 2195 2531 Fragments arranged by size: 2531 2195 368 138 110 14 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2108 2140 2339 2371 2430 Size: 32 135 32 536 32 199 32 59 Cuts at: 2430 2462 4009 4041 4083 4115 1373 Size: 32 1547 32 42 32 2626 Fragments arranged by size: 2626 1547 536 199 135 59 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2712 2958 669 Size: 988 281 774 246 3079 Fragments arranged by size: 3079 988 774 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5368 HpaI GTT'AAC Cuts at: 2958 2958 Size: 5368 KasI G'GCGC_C Cuts at: 2042 2042 Size: 5368 KpnI G_GTAC'C Cuts at: 2693 2693 Size: 5368 MfeI C'AATT_G Cuts at: 2967 2967 Size: 5368 MluI A'CGCG_T Cuts at: 5244 5244 Size: 5368 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4482 4666 1305 Size: 3177 184 2007 Fragments arranged by size: 3177 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2303 10 Size: 54 1425 343 471 3075 Fragments arranged by size: 3075 1425 471 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2450 3326 3685 3844 518 Size: 648 1284 876 359 159 2042 Fragments arranged by size: 2042 1284 876 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2272 2333 2369 2381 2391 2500 2784 Size: 417 249 61 36 12 10 109 284 Cuts at: 2784 3461 4609 4854 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 417 284 249 245 109 61 36 12 10 NarI GG'CG_CC Cuts at: 2043 2043 Size: 5368 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3877 Fragments arranged by size: 3877 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5368 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5368 NotI GC'GGCC_GC Cuts at: 2364 2815 2364 Size: 451 4917 NspI r_CATG'y Cuts at: 1132 1229 2726 4271 1132 Size: 97 1497 1545 2229 Fragments arranged by size: 2229 1545 1497 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5368 PciI A'CATG_T Cuts at: 4267 4267 Size: 5368 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5368 PmeI GTTT'AAAC Cuts at: 2672 2672 Size: 5368 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3437 3469 4950 4982 3437 Size: 32 1481 32 3823 Fragments arranged by size: 3823 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5368 PsiI TTA'TAA Cuts at: 2938 2938 Size: 5368 PstI C_TGCA'G Cuts at: 830 2076 2720 830 Size: 1246 644 3478 Fragments arranged by size: 3478 1246 644 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5368 PvuII CAG'CTG Cuts at: 2272 2272 Size: 5368 RsrII CG'GwC_CG Cuts at: 2025 2025 Size: 5368 SacI G_AGCT'C Cuts at: 2109 2532 2109 Size: 423 4945 SacII CC_GC'GG Cuts at: 2024 2334 2370 2024 Size: 310 36 5022 Fragments arranged by size: 5022 310 36 SalI G'TCGA_C Cuts at: 1936 2710 1936 Size: 774 4594 SbfI CC_TGCA'GG Cuts at: 2720 2720 Size: 5368 ScaI AGT'ACT Cuts at: 3616 5328 3616 Size: 1712 3656 SfcI C'TryA_G Cuts at: 826 1045 2072 2716 3851 4532 4723 826 Size: 219 1027 644 1135 681 191 1471 Fragments arranged by size: 1471 1135 1027 681 644 219 191 SfoI GGC'GCC Cuts at: 2044 2044 Size: 5368 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5368 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5368 SmaI CCC'GGG Cuts at: 1872 2184 2343 2375 2695 1872 Size: 312 159 32 320 4545 Fragments arranged by size: 4545 320 312 159 32 SmlI C'TyrA_G Cuts at: 820 1017 1943 3472 4373 4635 820 Size: 197 926 1529 901 262 1553 Fragments arranged by size: 1553 1529 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3159 493 Size: 2666 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5368 SphI G_CATG'C Cuts at: 1132 2726 1132 Size: 1594 3774 SrfI GCCC'GGGC Cuts at: 2184 2375 2184 Size: 191 5177 SspI AAT'ATT Cuts at: 5 52 3292 5 Size: 47 3240 2081 Fragments arranged by size: 3240 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5368 StyI C'CwwG_G Cuts at: 513 1065 2004 2523 2807 513 Size: 552 939 519 284 3074 Fragments arranged by size: 3074 939 552 519 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3395 3412 3565 3750 4195 325 Size: 1255 1815 17 153 185 445 1498 Fragments arranged by size: 1815 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3614 5326 96 Size: 274 80 33 51 1443 1637 1712 138 Fragments arranged by size: 1712 1637 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5328 5360 5328 Size: 32 5336 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5368 XbaI T'CTAG_A Cuts at: 2704 2704 Size: 5368 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2287 2530 2011 Size: 276 243 4849 Fragments arranged by size: 4849 276 243 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5368 XmaI C'CCGG_G Cuts at: 1870 2182 2341 2373 2693 1870 Size: 312 159 32 320 4545 Fragments arranged by size: 4545 320 312 159 32 XmnI GAAnn'nnTTC Cuts at: 3497 3497 Size: 5368 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3176 276 Size: 53 83 186 936 1642 2468 Fragments arranged by size: 2468 1642 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI SanDI SapI SexAI SfiI SwaI