(Linear) (Six-base) MAPSORT of: KIEE3933.seq Check: 496 from: 1 to: 663 KIEE3933.orf With 182 enzymes: SgfI * March 22, 2012 16:53 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 300 663 Size: 300 363 AfeI AGC'GCT Cuts at: 0 53 663 Size: 53 610 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 628 663 Size: 628 35 AlwNI CAG_nnn'CTG Cuts at: 0 143 379 663 Size: 143 236 284 Fragments arranged by size: 284 236 143 AvaI C'yCGr_G Cuts at: 0 177 336 368 663 Size: 177 159 32 295 Fragments arranged by size: 295 177 159 32 BanI G'GyrC_C Cuts at: 0 37 627 663 Size: 37 590 36 Fragments arranged by size: 590 37 36 BanII G_rGCy'C Cuts at: 0 104 251 527 663 Size: 104 147 276 136 Fragments arranged by size: 276 147 136 104 BbeI G_GCGC'C Cuts at: 0 41 663 Size: 41 622 BbvCI CC'TCA_GC Cuts at: 0 541 663 Size: 541 122 BmgBI CAC'GTC Cuts at: 0 83 663 Size: 83 580 BmrI ACTGGGnnnn_n' Cuts at: 0 254 663 Size: 254 409 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 583 663 Size: 583 80 Bpu10I CC'TnA_GC Cuts at: 0 541 663 Size: 541 122 BsaI GGTCTCn'nnnn_ Cuts at: 0 356 663 Size: 356 307 BsaHI Gr'CG_yC Cuts at: 0 38 663 Size: 38 625 BsaWI w'CCGG_w Cuts at: 0 581 663 Size: 581 82 BseYI C'CCAG_C Cuts at: 0 167 663 Size: 167 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 529 663 Size: 529 134 BsiEI CG_ry'CG Cuts at: 0 362 459 663 Size: 362 97 204 Fragments arranged by size: 362 204 97 BsiHKAI G_wGCw'C Cuts at: 0 88 104 347 473 482 527 663 Size: 88 16 243 126 9 45 136 Fragments arranged by size: 243 136 126 88 45 16 9 Bsp1286I G_dGCh'C Cuts at: 0 88 104 251 347 473 482 527 663 Size: 88 16 147 96 126 9 45 136 Fragments arranged by size: 147 136 126 96 88 45 16 9 BsrFI r'CCGG_y Cuts at: 0 22 34 114 663 Size: 22 12 80 549 Fragments arranged by size: 549 80 22 12 BssHII G'CGCG_C Cuts at: 0 209 253 663 Size: 209 44 410 Fragments arranged by size: 410 209 44 BstAPI GCAn_nnn'nTGC Cuts at: 0 379 486 663 Size: 379 107 177 Fragments arranged by size: 379 177 107 BstXI CCAn_nnnn'nTGG Cuts at: 0 447 663 Size: 447 216 BtgI C'CryG_G Cuts at: 0 16 326 362 587 643 663 Size: 16 310 36 225 56 20 Fragments arranged by size: 310 225 56 36 20 16 BtsI GCAGTG_nn' Cuts at: 0 223 244 663 Size: 223 21 419 Fragments arranged by size: 419 223 21 DraIII CAC_nnn'GTG Cuts at: 0 486 663 Size: 486 177 EaeI y'GGCC_r Cuts at: 0 132 296 359 663 Size: 132 164 63 304 Fragments arranged by size: 304 164 132 63 EagI C'GGCC_G Cuts at: 0 359 663 Size: 359 304 EcoICRI GAG'CTC Cuts at: 0 102 525 663 Size: 102 423 138 Fragments arranged by size: 423 138 102 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 300 583 663 Size: 300 283 80 Fragments arranged by size: 300 283 80 EcoO109I rG'GnC_Cy Cuts at: 0 637 663 Size: 637 26 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 543 663 Size: 543 120 HaeII r_GCGC'y Cuts at: 0 41 55 165 177 315 663 Size: 41 14 110 12 138 348 Fragments arranged by size: 348 138 110 41 14 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 103 135 334 366 425 457 663 Size: 103 32 199 32 59 32 206 Fragments arranged by size: 206 199 103 59 32 32 32 KasI G'GCGC_C Cuts at: 0 37 663 Size: 37 626 MscI TGG'CCA Cuts at: 0 298 663 Size: 298 365 MslI CAynn'nnrTG Cuts at: 0 445 663 Size: 445 218 MspA1I CmG'CkG Cuts at: 0 18 267 328 364 376 386 495 663 Size: 18 249 61 36 12 10 109 168 Fragments arranged by size: 249 168 109 61 36 18 12 10 NarI GG'CG_CC Cuts at: 0 38 663 Size: 38 625 NotI GC'GGCC_GC Cuts at: 0 359 663 Size: 359 304 PstI C_TGCA'G Cuts at: 0 71 663 Size: 71 592 PvuII CAG'CTG Cuts at: 0 267 663 Size: 267 396 RsrII CG'GwC_CG Cuts at: 0 20 663 Size: 20 643 SacI G_AGCT'C Cuts at: 0 104 527 663 Size: 104 423 136 Fragments arranged by size: 423 136 104 SacII CC_GC'GG Cuts at: 0 19 329 365 663 Size: 19 310 36 298 Fragments arranged by size: 310 298 36 19 SfcI C'TryA_G Cuts at: 0 67 663 Size: 67 596 SfoI GGC'GCC Cuts at: 0 39 663 Size: 39 624 SmaI CCC'GGG Cuts at: 0 179 338 370 663 Size: 179 159 32 293 Fragments arranged by size: 293 179 159 32 SrfI GCCC'GGGC Cuts at: 0 179 370 663 Size: 179 191 293 Fragments arranged by size: 293 191 179 StyI C'CwwG_G Cuts at: 0 518 663 Size: 518 145 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 282 525 663 Size: 282 243 138 Fragments arranged by size: 282 243 138 XmaI C'CCGG_G Cuts at: 0 177 336 368 663 Size: 177 159 32 295 Fragments arranged by size: 295 177 159 32 Enzymes that do cut: AcuI AfeI AlfI AlwNI AvaI BanI BanII BbeI BbvCI BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrFI BssHII BstAPI BstXI BtgI BtsI DraIII EaeI EagI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I KasI MscI MslI MspA1I NarI NotI PstI PvuII RsrII SacI SacII SfcI SfoI SmaI SrfI StyI XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmtI BplI BpuEI BsaAI BsaBI BsaXI BseRI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EarI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmnI ZraI