################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AccII AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AspI AvrII BamHI BcgI BclI BfaI BfrI BlnI BsiWI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FbaI HindIII HpaI KpnI KspI MaeI MluI MvnI NdeI NheI NotI NruI NspV PacI PflFI PinAI PmeI PshAI PvuI RsrII SacII SalI ScaI SfuI SgrAI SmaI SpeI SphI SspBI SspI StuI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccI 1306 GTAGAC GT^[AC][GT]AC AccIII 2246 TCCGGA T^CCGGA AclI 2830 AACGTT AA^CGTT AfaI 1458 GTAC GT^AC AocI 2289 CCTCAGG CC^T[ACGT]AGG ApaI 543 GGGCCC GGGCC^C AseI 1123 ATTAAT AT^TAAT AsnI 1123 ATTAAT AT^TAAT AspHI 1414 GAGCTC G[AT]GC[AT]^C AviII 1526 TGCGCA TGC^GCA BbeI 2593 GGCGCC GGCGC^C BbrPI 2697 CACGTG CAC^GTG BciVI 2243 GTATCC GTATCC(6/5) BglI 2278 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 271 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2062 GAGACC GGTCTC(1/5) BseAI 2246 TCCGGA T^CCGGA BsiEI 576 CGACCG CG[GA][CT]^CG BsiHKAI 1414 GAGCTC G[AT]GC[AT]^C BspEI 2246 TCCGGA T^CCGGA BspHI 1802 TCATGA T^CATGA BsrBI 2589 GAGCGG CCGCTC(-3/-3) BsrDI 2232 CATTGC GCAATG(2/0) BsrFI 938 GCCGGC [GA]^CCGG[CT] BstEII 1741 GGTGACC G^GT[ACGT]ACC BstPI 1741 GGTGACC G^GT[ACGT]ACC Bsu36I 2289 CCTCAGG CC^T[ACGT]AGG Cfr10I 938 GCCGGC [GA]^CCGG[CT] DrdI 781 GACCTTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 185 CCATGG C^C[GA][CT]GG Eco81I 2289 CCTCAGG CC^T[ACGT]AGG EcoO65I 1741 GGTGACC G^GT[ACGT]ACC EcoT22I 1076 ATGCAT ATGCA^T FseI 937 GGCCGGCC GGCCGG^CC FspI 1526 TGCGCA TGC^GCA HincII 610 GTCAAC GT[CT]^[GA]AC HindII 610 GTCAAC GT[CT]^[GA]AC KasI 2593 GGCGCC G^GCGCC MamI 271 GATGTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 167 CAATTG C^AATTG MroI 2246 TCCGGA T^CCGGA MunI 167 CAATTG C^AATTG NaeI 938 GCCGGC GCC^GGC NarI 2593 GGCGCC GG^CGCC NcoI 185 CCATGG C^CATGG NgoMIV 938 GCCGGC G^CCGGC NsiI 1076 ATGCAT ATGCA^T NspI 340 GCATGT [GA]CATG^[CT] PaeR7I 2190 CTCGAG C^TCGAG PmaCI 2697 CACGTG CAC^GTG PmlI 2697 CACGTG CAC^GTG PshBI 1123 ATTAAT AT^TAAT Psp1406I 2830 AACGTT AA^CGTT RcaI 1802 TCATGA T^CATGA RsaI 1458 GTAC GT^AC SacI 1414 GAGCTC GAGCT^C SbfI 2841 CCTGCAGG CCTGCA^GG SexAI 1640 ACCTGGT A^CC[AT]GGT SfiI 2277 GGCCCAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2593 GGCGCC GGC^GCC SnaBI 69 TACGTA TAC^GTA Sse8387I 2841 CCTGCAGG CCTGCA^GG XcmI 89 CCACCATCCCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2190 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcsI 471 AAATTT [GA]^AATT[CT] 1187 AAATTC AlwNI 1063 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1192 CAGAGCCTG ApoI 471 AAATTT [GA]^AATT[CT] 1187 AAATTC AvaI 2190 CTCGAG C^[CT]CG[GA]G 2887 CTCGGG BalI 2405 TGGCCA TGG^CCA 2861 TGGCCA BbvCI 2597 CCTCAGC CCTCAGC(-5/-2) 853 GCTGAGG BlpI 195 GCTGAGC GC^T[ACGT]AGC 1606 GCTGAGC Bpu1102I 195 GCTGAGC GC^T[ACGT]AGC 1606 GCTGAGC BsaAI 69 TACGTA [CT]AC^GT[GA] 2697 CACGTG BsaWI 578 ACCGGA [AT]^CCGG[AT] 2246 TCCGGA BsmBI 2088 CGTCTC CGTCTC(1/5) 972 GAGACG BsoBI 2190 CTCGAG C^[CT]CG[GA]G 2887 CTCGGG BspMI 2180 ACCTGC ACCTGC(4/8) 2763 GCAGGT CelII 195 GCTGAGC GC^T[ACGT]AGC 1606 GCTGAGC FauI 416 GCGGG CCCGC(4/6) 2445 GCGGG HaeII 2593 GGCGCC [GA]GCGC^[CT] 2850 GGCGCT HgaI 595 GACGC GACGC(5/10) 1167 GCGTC MluNI 2405 TGGCCA TGG^CCA 2861 TGGCCA MscI 2405 TGGCCA TGG^CCA 2861 TGGCCA PpuMI 1097 AGGACCC [GA]G^G[AT]CC[CT] 2099 GGGTCCC SmlI 1147 CTCAAG C^T[CT][GA]AG 2190 CTCGAG TfiI 978 GATTC G^A[AT]TC 1447 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AcyI AluI AlwI Asp700I AvaII BanI BanII BbsI BbvI BcnI BglII BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI PvuII SapI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI