(Circular) (Six-base) MAPSORT of: pFN21AE5066.seq Check: 6514 from: 1 to: 7609 pFN21AE5066 7609 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4194 4759 4194 Size: 565 7044 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5419 278 Size: 53 83 186 936 3883 2468 Fragments arranged by size: 3883 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3312 4952 1937 Size: 1375 1640 4594 Fragments arranged by size: 4594 1640 1375 Acc65I G'GTAC_C Cuts at: 4930 4930 Size: 7609 AclI AA'CG_TT Cuts at: 1563 4836 4908 5736 6109 1563 Size: 3273 72 828 373 3063 Fragments arranged by size: 3273 3063 828 373 72 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3116 3312 3981 5672 7056 7324 1632 Size: 1484 196 669 1691 1384 268 1917 Fragments arranged by size: 1917 1691 1484 1384 669 268 196 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7412 AflIII A'CryG_T Cuts at: 1203 6508 7485 1203 Size: 5305 977 1327 Fragments arranged by size: 5305 1327 977 AgeI A'CCGG_T Cuts at: 6440 6440 Size: 7609 AhdI GACnn_n'nnGTC Cuts at: 6338 6338 Size: 7609 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7609 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7609 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3530 3562 3530 Size: 32 7577 AlwNI CAG_nnn'CTG Cuts at: 3073 3202 5352 6924 3073 Size: 129 2150 1572 3758 Fragments arranged by size: 3758 2150 1572 129 ApaI G_GGCC'C Cuts at: 2552 2552 Size: 7609 ApaLI G'TGCA_C Cuts at: 5666 6822 5666 Size: 1156 6453 ApoI r'AATT_y Cuts at: 1493 2476 3192 4918 5144 5296 7405 1493 Size: 983 716 1726 226 152 2109 1697 Fragments arranged by size: 2109 1726 1697 983 716 226 152 AseI AT'TA_AT Cuts at: 160 1032 3129 6163 160 Size: 872 2097 3034 1606 Fragments arranged by size: 3034 2097 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7609 AvaI C'yCGr_G Cuts at: 1870 1943 4195 4892 4934 1870 Size: 73 2252 697 42 4545 Fragments arranged by size: 4545 2252 697 73 42 BamHI G'GATC_C Cuts at: 4939 5306 4939 Size: 367 7242 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2828 4274 4598 4930 6385 Size: 325 217 627 1041 1446 324 332 1455 Cuts at: 6385 618 Size: 1842 Fragments arranged by size: 1842 1455 1446 1041 627 332 325 324 217 BanII G_rGCy'C Cuts at: 1391 2312 2552 3423 3792 4928 1391 Size: 921 240 871 369 1136 4072 Fragments arranged by size: 4072 1136 921 871 369 240 BbeI G_GCGC'C Cuts at: 4602 4602 Size: 7609 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3390 3805 4541 928 Size: 971 1491 415 736 3996 Fragments arranged by size: 3996 1491 971 736 415 BbvCI CC'TCA_GC Cuts at: 2859 4603 2859 Size: 1744 5865 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5800 5834 1903 Size: 34 3863 34 3678 Fragments arranged by size: 3863 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4259 5501 6711 4259 Size: 1242 1210 5157 Fragments arranged by size: 5157 1242 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7609 BfrBI ATG'CAT Cuts at: 3083 3083 Size: 7609 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4289 6220 136 Size: 107 122 71 631 605 2617 1931 1525 Fragments arranged by size: 2617 1931 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2449 2466 2643 3627 7604 2449 Size: 17 177 984 3977 2454 Fragments arranged by size: 3977 2454 984 177 17 BlpI GC'TnA_GC Cuts at: 2201 3612 5026 2201 Size: 1411 1414 4784 Fragments arranged by size: 4784 1414 1411 Bme1580I G_kGCm'C Cuts at: 1790 2552 2825 2833 3430 5670 6826 1790 Size: 762 273 8 597 2240 1156 2573 Fragments arranged by size: 2573 2240 1156 762 597 273 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7609 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2853 4423 5340 Size: 416 53 83 399 24 1436 1570 917 Cuts at: 5340 6298 442 Size: 958 1753 Fragments arranged by size: 1753 1570 1436 958 917 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7609 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2660 3458 3466 4604 6269 1239 Size: 1421 798 8 1138 1665 2579 Fragments arranged by size: 2579 1665 1421 1138 798 8 Bpu10I CC'TnA_GC Cuts at: 2859 3090 3702 4603 2859 Size: 231 612 901 5865 Fragments arranged by size: 5865 901 612 231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3137 5734 6599 6897 3137 Size: 2597 865 298 3849 Fragments arranged by size: 3849 2597 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4061 6272 882 Size: 617 2562 2211 2219 Fragments arranged by size: 2562 2219 2211 617 BsaAI yAC'GTr Cuts at: 493 1204 2076 4704 5400 493 Size: 711 872 2628 696 2702 Fragments arranged by size: 2702 2628 872 711 696 BsaBI GATnn'nnATC Cuts at: 2280 7603 2280 Size: 5323 2286 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2601 3172 4599 5416 Size: 53 83 186 936 1068 571 1427 817 Cuts at: 5416 5798 6433 275 Size: 382 635 1451 Fragments arranged by size: 1451 1427 1068 936 817 635 571 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2583 4251 6042 6440 6714 6861 7272 2583 Size: 1668 1791 398 274 147 411 2920 Fragments arranged by size: 2920 1791 1668 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2970 3000 3530 3560 3705 Size: 30 165 30 1567 30 530 30 145 Cuts at: 3705 3735 4200 4230 1178 Size: 30 465 30 4557 Fragments arranged by size: 4557 1567 530 465 165 145 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2357 3233 3347 3726 1186 Size: 1171 876 114 379 5069 Fragments arranged by size: 5069 1171 876 379 114 BseYI C'CCAG_C Cuts at: 2237 2305 3348 4881 6812 7308 2237 Size: 68 1043 1533 1931 496 2538 Fragments arranged by size: 2538 1931 1533 1043 496 68 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3164 4397 4784 4800 1163 Size: 573 1428 1233 387 16 3972 Fragments arranged by size: 3972 1428 1233 573 387 16 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2584 5059 5820 6848 1540 Size: 275 186 583 2475 761 1028 2301 Fragments arranged by size: 2475 2301 1028 761 583 275 186 BsiHKAI G_wGCw'C Cuts at: 3423 5670 5755 6826 3423 Size: 2247 85 1071 4206 Fragments arranged by size: 4206 2247 1071 85 BsmI GAATG_Cn' Cuts at: 2385 2462 2688 3703 4732 5120 5213 2385 Size: 77 226 1015 1029 388 93 4781 Fragments arranged by size: 4781 1029 1015 388 226 93 77 BsmBI CGTCTCn'nnnn_ Cuts at: 2971 4099 2971 Size: 1128 6481 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2312 2552 2825 2833 3423 3430 3792 Size: 399 522 240 273 8 590 7 362 Cuts at: 3792 4928 5670 5755 6826 1391 Size: 1136 742 85 1071 2174 Fragments arranged by size: 2174 1136 1071 742 590 522 399 362 273 240 85 8 7 BspEI T'CCGG_A Cuts at: 4251 7272 4251 Size: 3021 4588 BspHI T'CATG_A Cuts at: 3807 5498 7228 3807 Size: 1691 1730 4188 Fragments arranged by size: 4188 1730 1691 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4194 4759 4964 844 Size: 3350 565 205 3489 Fragments arranged by size: 3489 3350 565 205 BsrBI CCG'CTC Cuts at: 1394 1823 4596 5496 1394 Size: 429 2773 900 3507 Fragments arranged by size: 3507 2773 900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4236 6104 6278 65 Size: 1183 2988 1868 174 1396 Fragments arranged by size: 2988 1868 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2943 6253 6440 1700 Size: 110 1133 3310 187 2869 Fragments arranged by size: 3310 2869 1133 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7609 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7609 BssSI C'ACGA_G Cuts at: 5669 6681 5669 Size: 1012 6597 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7609 BstBI TT'CG_AA Cuts at: 6413 6413 Size: 7609 BstEII G'GTnAC_C Cuts at: 1184 3746 1184 Size: 2562 5047 BstXI CCAn_nnnn'nTGG Cuts at: 2104 2381 2923 3578 2104 Size: 277 542 655 6135 Fragments arranged by size: 6135 655 542 277 BstYI r'GATC_y Cuts at: 1972 2449 2466 2571 2643 3271 3627 4939 5306 Size: 477 17 105 72 628 356 1312 367 Cuts at: 5306 5691 5708 7160 7246 7258 7604 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1312 628 477 385 367 356 346 105 86 72 17 17 12 Bsu36I CC'TnA_GG Cuts at: 4295 4295 Size: 7609 BtgI C'CryG_G Cuts at: 513 1065 2004 2190 513 Size: 552 939 186 5932 Fragments arranged by size: 5932 939 552 186 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6870 Fragments arranged by size: 6870 683 56 BtsI GCAGTG_nn' Cuts at: 2572 3231 3673 4413 5125 5919 5939 2572 Size: 659 442 740 712 794 20 4242 Fragments arranged by size: 4242 794 740 712 659 442 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7609 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7574 DraI TTT'AAA Cuts at: 4913 4913 Size: 7609 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2792 6616 809 Size: 507 1476 3824 1802 Fragments arranged by size: 3824 1802 1476 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2941 4410 4866 Size: 54 1425 50 275 18 1111 1469 456 Cuts at: 4866 5056 5945 7574 8 Size: 190 889 1629 43 Fragments arranged by size: 1629 1469 1425 1111 889 456 275 190 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5056 1537 Size: 275 3244 4090 Fragments arranged by size: 4090 3244 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2706 3132 3288 3324 3411 3549 3957 4640 Size: 1353 426 156 36 87 138 408 683 Cuts at: 4640 4676 5539 1353 Size: 36 863 3423 Fragments arranged by size: 3423 1353 863 683 426 408 156 138 87 36 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2978 6196 6570 6716 387 Size: 1064 138 1389 3218 374 146 1280 Fragments arranged by size: 3218 1389 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3421 3421 Size: 7609 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2660 3116 3312 3458 3466 3981 4604 Size: 393 1028 456 196 146 8 515 623 Cuts at: 4604 5672 6269 7056 7324 1239 Size: 1068 597 787 268 1524 Fragments arranged by size: 1524 1068 1028 787 623 597 515 456 393 268 196 146 8 EcoNI CCTnn'n_nnAGG Cuts at: 3650 4400 4472 4850 3650 Size: 750 72 378 6409 Fragments arranged by size: 6409 750 378 72 EcoO109I rG'GnC_Cy Cuts at: 2549 3103 3301 4045 4105 4291 4405 4612 2549 Size: 554 198 744 60 186 114 207 5546 Fragments arranged by size: 5546 744 554 207 198 186 114 60 EcoRI G'AATT_C Cuts at: 4918 5296 4918 Size: 378 7231 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2542 2542 Size: 7609 FseI GG_CCGG'CC Cuts at: 2947 2947 Size: 7609 FspI TGC'GCA Cuts at: 3533 6115 3533 Size: 2582 5027 HaeII r_GCGC'y Cuts at: 1678 4602 4859 6756 1678 Size: 2924 257 1897 2531 Fragments arranged by size: 2924 2531 1897 257 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2578 2610 2970 3002 3612 Size: 32 135 32 1006 32 360 32 610 Cuts at: 3612 3644 4118 4150 6250 6282 6324 6356 1373 Size: 32 474 32 2100 32 42 32 2626 Fragments arranged by size: 2626 2100 1006 610 474 360 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2617 4953 5199 669 Size: 988 281 679 2336 246 3079 Fragments arranged by size: 3079 2336 988 679 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7609 HpaI GTT'AAC Cuts at: 5199 5199 Size: 7609 KasI G'GCGC_C Cuts at: 4598 4598 Size: 7609 KpnI G_GTAC'C Cuts at: 4934 4934 Size: 7609 MfeI C'AATT_G Cuts at: 2172 5208 2172 Size: 3036 4573 MluI A'CGCG_T Cuts at: 7485 7485 Size: 7609 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2796 6723 6907 1305 Size: 1491 3927 184 2007 Fragments arranged by size: 3927 2007 1491 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4412 4868 10 Size: 54 1425 343 2580 456 2751 Fragments arranged by size: 2751 2580 1425 456 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2076 2726 2921 5567 5926 6085 518 Size: 648 910 650 195 2646 359 159 2042 Fragments arranged by size: 2646 2042 910 650 648 359 195 159 MspA1I CmG'CkG Cuts at: 1606 2435 3352 4864 4885 5025 5702 6850 7095 Size: 829 917 1512 21 140 677 1148 245 Cuts at: 7095 1606 Size: 2120 Fragments arranged by size: 2120 1512 1148 917 829 677 245 140 21 NaeI GCC'GGC Cuts at: 2945 2945 Size: 7609 NarI GG'CG_CC Cuts at: 4599 4599 Size: 7609 NcoI C'CATG_G Cuts at: 513 1065 2004 2190 513 Size: 552 939 186 5932 Fragments arranged by size: 5932 939 552 186 NdeI CA'TA_TG Cuts at: 387 387 Size: 7609 NgoMIV G'CCGG_C Cuts at: 2943 2943 Size: 7609 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7609 NotI GC'GGCC_GC Cuts at: 5056 5056 Size: 7609 NsiI A_TGCA'T Cuts at: 3085 3085 Size: 7609 NspI r_CATG'y Cuts at: 1132 1229 2349 4967 6512 1132 Size: 97 1120 2618 1545 2229 Fragments arranged by size: 2618 2229 1545 1120 97 PasI CC'CwG_GG Cuts at: 1348 3073 1348 Size: 1725 5884 PciI A'CATG_T Cuts at: 6508 6508 Size: 7609 PflMI CCAn_nnn'nTGG Cuts at: 1108 2843 3073 4758 1108 Size: 1735 230 1685 3959 Fragments arranged by size: 3959 1735 1685 230 PfoI T'CCnGG_A Cuts at: 3180 3223 4581 3180 Size: 43 1358 6208 Fragments arranged by size: 6208 1358 43 PmeI GTTT'AAAC Cuts at: 4913 4913 Size: 7609 PmlI CAC'GTG Cuts at: 4704 4704 Size: 7609 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3530 3562 5678 5710 7191 7223 3530 Size: 32 2116 32 1481 32 3916 Fragments arranged by size: 3916 2116 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3103 4105 3103 Size: 1002 6607 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7609 PsiI TTA'TAA Cuts at: 5179 5179 Size: 7609 PspOMI G'GGCC_C Cuts at: 2548 2548 Size: 7609 PspXI vC'TCGA_Gb Cuts at: 4195 4195 Size: 7609 PstI C_TGCA'G Cuts at: 830 2760 4420 4851 4863 4961 830 Size: 1930 1660 431 12 98 3478 Fragments arranged by size: 3478 1930 1660 431 98 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7609 PvuII CAG'CTG Cuts at: 2435 3352 4864 4885 2435 Size: 917 1512 21 5159 Fragments arranged by size: 5159 1512 917 21 SacI G_AGCT'C Cuts at: 3423 3423 Size: 7609 SalI G'TCGA_C Cuts at: 1936 4951 1936 Size: 3015 4594 SanDI GG'GwC_CC Cuts at: 4105 4105 Size: 7609 SapI GCTCTTCn'nnn_ Cuts at: 3411 4640 4676 3411 Size: 1229 36 6344 Fragments arranged by size: 6344 1229 36 SbfI CC_TGCA'GG Cuts at: 4851 4961 4851 Size: 110 7499 ScaI AGT'ACT Cuts at: 5857 7569 5857 Size: 1712 5897 SexAI A'CCwGG_T Cuts at: 3645 3645 Size: 7609 SfcI C'TryA_G Cuts at: 826 1045 2756 4416 4847 4859 4957 6092 6773 Size: 219 1711 1660 431 12 98 1135 681 Cuts at: 6773 6964 826 Size: 191 1471 Fragments arranged by size: 1711 1660 1471 1135 681 431 219 191 98 12 SfiI GGCCn_nnn'nGGCC Cuts at: 4289 4289 Size: 7609 SfoI GGC'GCC Cuts at: 4600 4600 Size: 7609 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7609 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7609 SmaI CCC'GGG Cuts at: 1872 4936 1872 Size: 3064 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3152 4195 5713 6614 6876 820 Size: 197 926 1209 1043 1518 901 262 1553 Fragments arranged by size: 1553 1518 1209 1043 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2076 5400 493 Size: 1583 3324 2702 Fragments arranged by size: 3324 2702 1583 SpeI A'CTAG_T Cuts at: 152 152 Size: 7609 SphI G_CATG'C Cuts at: 1132 4967 1132 Size: 3835 3774 SspI AAT'ATT Cuts at: 5 52 5533 5 Size: 47 5481 2081 Fragments arranged by size: 5481 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7609 StyI C'CwwG_G Cuts at: 513 1065 2004 2190 3924 3993 4242 4396 4608 Size: 552 939 186 1734 69 249 154 212 Cuts at: 4608 5048 513 Size: 440 3074 Fragments arranged by size: 3074 1734 939 552 440 249 212 186 154 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2387 2528 3956 4509 4750 5636 5653 Size: 1255 807 141 1428 553 241 886 17 Cuts at: 5653 5806 5991 6436 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1428 1255 886 807 553 445 241 185 153 141 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3462 5855 7567 Size: 274 80 33 51 1443 1485 2393 1712 Cuts at: 7567 96 Size: 138 Fragments arranged by size: 2393 1712 1485 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2839 2871 3705 3737 4200 4232 7569 7601 2839 Size: 32 834 32 463 32 3337 32 2847 Fragments arranged by size: 3337 2847 834 463 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7609 XbaI T'CTAG_A Cuts at: 4945 4945 Size: 7609 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2101 2101 Size: 7609 XhoI C'TCGA_G Cuts at: 1943 4195 1943 Size: 2252 5357 XmaI C'CCGG_G Cuts at: 1870 4934 1870 Size: 3064 4545 XmnI GAAnn'nnTTC Cuts at: 2147 2811 3545 4544 4634 5738 2147 Size: 664 734 999 90 1104 4018 Fragments arranged by size: 4018 1104 999 734 664 90 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5417 276 Size: 53 83 186 936 3883 2468 Fragments arranged by size: 3883 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BplI BsiWI BstZ17I DraIII EcoRV FspAI NruI PacI PsrI RsrII SacII SrfI SwaI