(Linear) (Six-base) MAPSORT of: KIEE5066.seq Check: 8479 from: 1 to: 2904 KIEE5066.orf With 182 enzymes: SgfI * December 5, 2014 12:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2189 2754 2904 Size: 2189 565 150 Fragments arranged by size: 2189 565 150 AccI GT'mk_AC Cuts at: 0 1307 2904 Size: 1307 1597 AclI AA'CG_TT Cuts at: 0 2831 2904 Size: 2831 73 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1111 1307 1976 2904 Size: 1111 196 669 928 Fragments arranged by size: 1111 928 669 196 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1525 1557 2904 Size: 1525 32 1347 Fragments arranged by size: 1525 1347 32 AlwNI CAG_nnn'CTG Cuts at: 0 1068 1197 2904 Size: 1068 129 1707 Fragments arranged by size: 1707 1068 129 ApaI G_GGCC'C Cuts at: 0 547 2904 Size: 547 2357 ApoI r'AATT_y Cuts at: 0 471 1187 2904 Size: 471 716 1717 Fragments arranged by size: 1717 716 471 AseI AT'TA_AT Cuts at: 0 1124 2904 Size: 1124 1780 AvaI C'yCGr_G Cuts at: 0 2190 2887 2904 Size: 2190 697 17 Fragments arranged by size: 2190 697 17 BanI G'GyrC_C Cuts at: 0 823 2269 2593 2904 Size: 823 1446 324 311 Fragments arranged by size: 1446 823 324 311 BanII G_rGCy'C Cuts at: 0 307 547 1418 1787 2904 Size: 307 240 871 369 1117 Fragments arranged by size: 1117 871 369 307 240 BbeI G_GCGC'C Cuts at: 0 2597 2904 Size: 2597 307 BbsI GAAGACnn'nnnn_ Cuts at: 0 1385 1800 2536 2904 Size: 1385 415 736 368 Fragments arranged by size: 1385 736 415 368 BbvCI CC'TCA_GC Cuts at: 0 854 2598 2904 Size: 854 1744 306 Fragments arranged by size: 1744 854 306 BciVI GTATCCnnnnn_n' Cuts at: 0 2254 2904 Size: 2254 650 BfrBI ATG'CAT Cuts at: 0 1078 2904 Size: 1078 1826 BglI GCCn_nnn'nGGC Cuts at: 0 2284 2904 Size: 2284 620 BglII A'GATC_T Cuts at: 0 444 461 638 1622 2904 Size: 444 17 177 984 1282 Fragments arranged by size: 1282 984 444 177 17 BlpI GC'TnA_GC Cuts at: 0 196 1607 2904 Size: 196 1411 1297 Fragments arranged by size: 1411 1297 196 Bme1580I G_kGCm'C Cuts at: 0 547 820 828 1425 2904 Size: 547 273 8 597 1479 Fragments arranged by size: 1479 597 547 273 8 BmrI ACTGGGnnnn_n' Cuts at: 0 848 2418 2904 Size: 848 1570 486 Fragments arranged by size: 1570 848 486 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 655 1453 1461 2599 2904 Size: 655 798 8 1138 305 Fragments arranged by size: 1138 798 655 305 8 Bpu10I CC'TnA_GC Cuts at: 0 854 1085 1697 2598 2904 Size: 854 231 612 901 306 Fragments arranged by size: 901 854 612 306 231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1132 2904 Size: 1132 1772 BsaI GGTCTCn'nnnn_ Cuts at: 0 2056 2904 Size: 2056 848 BsaAI yAC'GTr Cuts at: 0 71 2699 2904 Size: 71 2628 205 Fragments arranged by size: 2628 205 71 BsaBI GATnn'nnATC Cuts at: 0 275 2904 Size: 275 2629 BsaHI Gr'CG_yC Cuts at: 0 596 1167 2594 2904 Size: 596 571 1427 310 Fragments arranged by size: 1427 596 571 310 BsaWI w'CCGG_w Cuts at: 0 578 2246 2904 Size: 578 1668 658 Fragments arranged by size: 1668 658 578 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 965 995 1525 1555 1700 1730 2195 2225 Size: 965 30 530 30 145 30 465 30 Cuts at: 2225 2904 Size: 679 Fragments arranged by size: 965 679 530 465 145 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 352 1228 1342 1721 2904 Size: 352 876 114 379 1183 Fragments arranged by size: 1183 876 379 352 114 BseYI C'CCAG_C Cuts at: 0 232 300 1343 2876 2904 Size: 232 68 1043 1533 28 Fragments arranged by size: 1533 1043 232 68 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1159 2392 2779 2795 2904 Size: 1159 1233 387 16 109 Fragments arranged by size: 1233 1159 387 109 16 BsiEI CG_ry'CG Cuts at: 0 579 2904 Size: 579 2325 BsiHKAI G_wGCw'C Cuts at: 0 1418 2904 Size: 1418 1486 BsmI GAATG_Cn' Cuts at: 0 380 457 683 1698 2727 2904 Size: 380 77 226 1015 1029 177 Fragments arranged by size: 1029 1015 380 226 177 77 BsmBI CGTCTCn'nnnn_ Cuts at: 0 966 2094 2904 Size: 966 1128 810 Fragments arranged by size: 1128 966 810 Bsp1286I G_dGCh'C Cuts at: 0 307 547 820 828 1418 1425 1787 2904 Size: 307 240 273 8 590 7 362 1117 Fragments arranged by size: 1117 590 362 307 273 240 8 7 BspEI T'CCGG_A Cuts at: 0 2246 2904 Size: 2246 658 BspHI T'CATG_A Cuts at: 0 1802 2904 Size: 1802 1102 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 2189 2754 2904 Size: 2189 565 150 Fragments arranged by size: 2189 565 150 BsrBI CCG'CTC Cuts at: 0 2591 2904 Size: 2591 313 BsrDI GCAATG_nn' Cuts at: 0 2231 2904 Size: 2231 673 BsrFI r'CCGG_y Cuts at: 0 938 2904 Size: 938 1966 BstEII G'GTnAC_C Cuts at: 0 1741 2904 Size: 1741 1163 BstXI CCAn_nnnn'nTGG Cuts at: 0 99 376 918 1573 2904 Size: 99 277 542 655 1331 Fragments arranged by size: 1331 655 542 277 99 BstYI r'GATC_y Cuts at: 0 444 461 566 638 1266 1622 2904 Size: 444 17 105 72 628 356 1282 Fragments arranged by size: 1282 628 444 356 105 72 17 Bsu36I CC'TnA_GG Cuts at: 0 2290 2904 Size: 2290 614 BtgI C'CryG_G Cuts at: 0 185 2904 Size: 185 2719 BtsI GCAGTG_nn' Cuts at: 0 567 1226 1668 2408 2904 Size: 567 659 442 740 496 Fragments arranged by size: 740 659 567 496 442 DrdI GACnn_nn'nnGTC Cuts at: 0 787 2904 Size: 787 2117 EaeI y'GGCC_r Cuts at: 0 936 2405 2861 2904 Size: 936 1469 456 43 Fragments arranged by size: 1469 936 456 43 EarI CTCTTCn'nnn_ Cuts at: 0 701 1127 1283 1319 1406 1544 1952 2635 Size: 701 426 156 36 87 138 408 683 Cuts at: 2635 2671 2904 Size: 36 233 Fragments arranged by size: 701 683 426 408 233 156 138 87 36 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 973 2904 Size: 973 1931 EcoICRI GAG'CTC Cuts at: 0 1416 2904 Size: 1416 1488 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 655 1111 1307 1453 1461 1976 2599 2904 Size: 655 456 196 146 8 515 623 305 Fragments arranged by size: 655 623 515 456 305 196 146 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 1645 2395 2467 2845 2904 Size: 1645 750 72 378 59 Fragments arranged by size: 1645 750 378 72 59 EcoO109I rG'GnC_Cy Cuts at: 0 544 1098 1296 2040 2100 2286 2400 2607 Size: 544 554 198 744 60 186 114 207 Cuts at: 2607 2904 Size: 297 Fragments arranged by size: 744 554 544 297 207 198 186 114 60 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 537 2904 Size: 537 2367 FseI GG_CCGG'CC Cuts at: 0 942 2904 Size: 942 1962 FspI TGC'GCA Cuts at: 0 1528 2904 Size: 1528 1376 HaeII r_GCGC'y Cuts at: 0 2597 2854 2904 Size: 2597 257 50 Fragments arranged by size: 2597 257 50 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 573 605 965 997 1607 1639 2113 2145 Size: 573 32 360 32 610 32 474 32 Cuts at: 2145 2904 Size: 759 Fragments arranged by size: 759 610 573 474 360 32 32 32 32 HincII GTy'rAC Cuts at: 0 612 2904 Size: 612 2292 KasI G'GCGC_C Cuts at: 0 2593 2904 Size: 2593 311 MfeI C'AATT_G Cuts at: 0 167 2904 Size: 167 2737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 791 2904 Size: 791 2113 MscI TGG'CCA Cuts at: 0 2407 2863 2904 Size: 2407 456 41 Fragments arranged by size: 2407 456 41 MslI CAynn'nnrTG Cuts at: 0 71 721 916 2904 Size: 71 650 195 1988 Fragments arranged by size: 1988 650 195 71 MspA1I CmG'CkG Cuts at: 0 430 1347 2859 2880 2904 Size: 430 917 1512 21 24 Fragments arranged by size: 1512 917 430 24 21 NaeI GCC'GGC Cuts at: 0 940 2904 Size: 940 1964 NarI GG'CG_CC Cuts at: 0 2594 2904 Size: 2594 310 NcoI C'CATG_G Cuts at: 0 185 2904 Size: 185 2719 NgoMIV G'CCGG_C Cuts at: 0 938 2904 Size: 938 1966 NsiI A_TGCA'T Cuts at: 0 1080 2904 Size: 1080 1824 NspI r_CATG'y Cuts at: 0 344 2904 Size: 344 2560 PasI CC'CwG_GG Cuts at: 0 1068 2904 Size: 1068 1836 PflMI CCAn_nnn'nTGG Cuts at: 0 838 1068 2753 2904 Size: 838 230 1685 151 Fragments arranged by size: 1685 838 230 151 PfoI T'CCnGG_A Cuts at: 0 1175 1218 2576 2904 Size: 1175 43 1358 328 Fragments arranged by size: 1358 1175 328 43 PmlI CAC'GTG Cuts at: 0 2699 2904 Size: 2699 205 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1525 1557 2904 Size: 1525 32 1347 Fragments arranged by size: 1525 1347 32 PpuMI rG'GwC_Cy Cuts at: 0 1098 2100 2904 Size: 1098 1002 804 Fragments arranged by size: 1098 1002 804 PspOMI G'GGCC_C Cuts at: 0 543 2904 Size: 543 2361 PspXI vC'TCGA_Gb Cuts at: 0 2190 2904 Size: 2190 714 PstI C_TGCA'G Cuts at: 0 755 2415 2846 2858 2904 Size: 755 1660 431 12 46 Fragments arranged by size: 1660 755 431 46 12 PvuII CAG'CTG Cuts at: 0 430 1347 2859 2880 2904 Size: 430 917 1512 21 24 Fragments arranged by size: 1512 917 430 24 21 SacI G_AGCT'C Cuts at: 0 1418 2904 Size: 1418 1486 SanDI GG'GwC_CC Cuts at: 0 2100 2904 Size: 2100 804 SapI GCTCTTCn'nnn_ Cuts at: 0 1406 2635 2671 2904 Size: 1406 1229 36 233 Fragments arranged by size: 1406 1229 233 36 SbfI CC_TGCA'GG Cuts at: 0 2846 2904 Size: 2846 58 SexAI A'CCwGG_T Cuts at: 0 1640 2904 Size: 1640 1264 SfcI C'TryA_G Cuts at: 0 751 2411 2842 2854 2904 Size: 751 1660 431 12 50 Fragments arranged by size: 1660 751 431 50 12 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2284 2904 Size: 2284 620 SfoI GGC'GCC Cuts at: 0 2595 2904 Size: 2595 309 SmlI C'TyrA_G Cuts at: 0 1147 2190 2904 Size: 1147 1043 714 Fragments arranged by size: 1147 1043 714 SnaBI TAC'GTA Cuts at: 0 71 2904 Size: 71 2833 StyI C'CwwG_G Cuts at: 0 185 1919 1988 2237 2391 2603 2904 Size: 185 1734 69 249 154 212 301 Fragments arranged by size: 1734 301 249 212 185 154 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 382 523 1951 2504 2745 2904 Size: 382 141 1428 553 241 159 Fragments arranged by size: 1428 553 382 241 159 141 TatI w'GTAC_w Cuts at: 0 1457 2904 Size: 1457 1447 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 834 866 1700 1732 2195 2227 2904 Size: 834 32 834 32 463 32 677 Fragments arranged by size: 834 834 677 463 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 96 2904 Size: 96 2808 XhoI C'TCGA_G Cuts at: 0 2190 2904 Size: 2190 714 XmnI GAAnn'nnTTC Cuts at: 0 142 806 1540 2539 2629 2904 Size: 142 664 734 999 90 275 Fragments arranged by size: 999 734 664 275 142 90 Enzymes that do cut: AarI AccI AclI AcuI AloI AlwNI ApaI ApoI AseI AvaI BanI BanII BbeI BbsI BbvCI BciVI BfrBI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SacI SanDI SapI SbfI SexAI SfcI SfiI SfoI SmlI SnaBI StyI TaqII TatI TstI XcmI XhoI XmnI Enzymes that do not cut: AatII Acc65I AfeI AflII AflIII AgeI AhdI AleI AlfI ApaLI AscI AsiSI AvrII BaeI BamHI BcgI BclI BmgBI BmtI BplI BsiWI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII EagI EcoRI EcoRV FspAI HindIII HpaI KpnI MluI NdeI NheI NotI NruI PacI PciI PmeI PshAI PsiI PsrI PvuI RsrII SacII SalI ScaI SgfI SgrAI SmaI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XmaI ZraI