################################## # NO CUT ENZYMES ( 158 enzymes) # ################################## AatII AccI AccII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MaeI MaeII MamI MluI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 651 GGTACC G^GTACC AccIII 761 TCCGGA T^CCGGA AciI 627 CCGC CCGC(-3/-1) AcsI 250 GAATTT [GA]^AATT[CT] AlwI 324 GATCC GGATC(4/5) ApoI 250 GAATTT [GA]^AATT[CT] Asp718I 651 GGTACC G^GTACC AvaI 230 CTCGGG C^[CT]CG[GA]G AvaII 195 GGACC G^G[AT]CC BalI 103 TGGCCA TGG^CCA BanII 263 GGGCTC G[GA]GC[CT]^C BglII 675 AGATCT A^GATCT BmyI 263 GGGCTC G[AGT]GC[ACT]^C BsaWI 761 TCCGGA [AT]^CCGG[AT] BseAI 761 TCCGGA T^CCGGA BsgI 133 CTGCAC GTGCAG(16/14) BsmAI 526 GTCTC GTCTC(1/5) BsoBI 230 CTCGGG C^[CT]CG[GA]G Bsp1286I 263 GGGCTC G[AGT]GC[ACT]^C BspEI 761 TCCGGA T^CCGGA BspHI 6 TCATGA T^CATGA BspMI 113 ACCTGC ACCTGC(4/8) BstYI 675 AGATCT [GA]^GATC[CT] CfoI 28 GCGC GCG^C DraIII 136 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 103 TGGCCA [CT]^GGCC[GA] EarI 454 CTCTTC CTCTTC(1/4) EcoT14I 18 CCAAGG C^C[AT][AT]GG HhaI 28 GCGC GCG^C HinP1I 28 GCGC G^CGC HphI 778 GGTGA GGTGA(8/7) KpnI 651 GGTACC GGTAC^C Ksp632I 454 CTCTTC CTCTTC(1/4) MfeI 638 CAATTG C^AATTG MflI 675 AGATCT [GA]^GATC[CT] MluNI 103 TGGCCA TGG^CCA MroI 761 TCCGGA T^CCGGA MscI 103 TGGCCA TGG^CCA MseI 568 TTAA T^TAA MspA1I 530 CAGCTG C[AC]G^C[GT]G MunI 638 CAATTG C^AATTG PvuII 530 CAGCTG CAG^CTG RcaI 6 TCATGA T^CATGA SfcI 34 CTACAG C^T[GA][CT]AG StyI 18 CCAAGG C^C[AT][AT]GG Tru9I 568 TTAA T^TAA XhoII 675 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 144 GTAC GT^AC 652 GTAC BanI 234 GGTGCC G^G[CT][GA]CC 651 GGTACC BcnI 360 CCCGG CC^[GC]GG 656 CCCGG BpmI 409 CTGGAG CTGGAG(16/14) 682 CTGGAG BstF5I 153 GGATG GGATG(2/0) 320 GGATG BstXI 46 CCACTTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 425 CCAACACTTTGG Cac8I 242 GCCTGC GC[ACGT]^[ACGT]GC 419 GCAAGC DpnII 324 GATC ^GATC 676 GATC EcoNI 499 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 769 CCTTGCTCAGG FokI 153 GGATG GGATG(9/13) 320 GGATG MboI 324 GATC ^GATC 676 GATC NciI 360 CCCGG CC^[GC]GG 656 CCCGG NdeII 324 GATC ^GATC 676 GATC NlaIII 7 CATG CATG^ 465 CATG NlaIV 234 GGTGCC GG[ACGT]^[ACGT]CC 651 GGTACC RsaI 144 GTAC GT^AC 652 GTAC Sau3AI 324 GATC ^GATC 676 GATC StuI 182 AGGCCT AGG^CCT 554 AGGCCT TaqI 212 TCGA T^CGA 536 TCGA Tsp45I 610 GTGAC ^GT[GC]AC 779 GTGAC TthHB8I 212 TCGA T^CGA 536 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AluI BbvI BsaJI BsiYI BslI BsmFI BsrI BssKI BstNI Cfr13I DdeI EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI MaeIII MboII MnlI MslI MspI MvaI MwoI PleI Sau96I ScrFI SfaNI TseI Tsp509I TspRI