(Circular) (Six-base) MAPSORT of: pFN21AE1343.seq Check: 8388 from: 1 to: 5488 pFN21AE1343 5488 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3298 278 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2831 1937 Size: 894 4594 Acc65I G'GTAC_C Cuts at: 2656 2809 2656 Size: 153 5335 AclI AA'CG_TT Cuts at: 1563 3615 3988 1563 Size: 2052 373 3063 Fragments arranged by size: 3063 2052 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2109 2412 2426 3551 4935 5203 1632 Size: 477 303 14 1125 1384 268 1917 Fragments arranged by size: 1917 1384 1125 477 303 268 14 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5291 AflIII A'CryG_T Cuts at: 1203 4387 5364 1203 Size: 3184 977 1327 Fragments arranged by size: 3184 1327 977 AgeI A'CCGG_T Cuts at: 4319 4319 Size: 5488 AhdI GACnn_n'nnGTC Cuts at: 4217 4217 Size: 5488 AleI CACnn'nnGTG Cuts at: 1166 2056 2237 1166 Size: 890 181 4417 Fragments arranged by size: 4417 890 181 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5488 AlwNI CAG_nnn'CTG Cuts at: 3231 4803 3231 Size: 1572 3916 ApaLI G'TGCA_C Cuts at: 3545 4701 3545 Size: 1156 4332 ApoI r'AATT_y Cuts at: 1493 2255 2797 3023 3175 5284 1493 Size: 762 542 226 152 2109 1697 Fragments arranged by size: 2109 1697 762 542 226 152 AseI AT'TA_AT Cuts at: 160 1032 4042 160 Size: 872 3010 1606 Fragments arranged by size: 3010 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 AvaI C'yCGr_G Cuts at: 1870 1943 2235 2813 1870 Size: 73 292 578 4545 Fragments arranged by size: 4545 578 292 73 BamHI G'GATC_C Cuts at: 2818 3185 2818 Size: 367 5121 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2239 2656 2809 4264 618 Size: 325 217 627 452 417 153 1455 1842 Fragments arranged by size: 1842 1455 627 452 417 325 217 153 BanII G_rGCy'C Cuts at: 1391 2272 2807 1391 Size: 881 535 4072 Fragments arranged by size: 4072 881 535 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4517 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3679 3713 1903 Size: 34 1742 34 3678 Fragments arranged by size: 3678 1742 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3380 4590 3380 Size: 1210 4278 BclI T'GATC_A Cuts at: 1548 1548 Size: 5488 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4099 136 Size: 107 122 71 631 605 2427 1525 Fragments arranged by size: 2427 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2680 5483 2680 Size: 2803 2685 BlpI GC'TnA_GC Cuts at: 2905 2905 Size: 5488 Bme1580I G_kGCm'C Cuts at: 1790 3549 4705 1790 Size: 1759 1156 2573 Fragments arranged by size: 2573 1759 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5488 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2163 2275 3219 Size: 416 53 83 399 24 746 112 944 Cuts at: 3219 4177 442 Size: 958 1753 Fragments arranged by size: 1753 958 944 746 416 399 112 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2435 2708 4148 1239 Size: 1196 273 1440 2579 Fragments arranged by size: 2579 1440 1196 273 Bpu10I CC'TnA_GC Cuts at: 2271 2779 2271 Size: 508 4980 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3613 4478 4776 3613 Size: 865 298 4325 Fragments arranged by size: 4325 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4151 882 Size: 617 2652 2219 Fragments arranged by size: 2652 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3279 493 Size: 711 2075 2702 Fragments arranged by size: 2702 2075 711 BsaBI GATnn'nnATC Cuts at: 5482 5482 Size: 5488 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3295 3677 4312 275 Size: 53 83 186 936 1762 382 635 1451 Fragments arranged by size: 1762 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2766 3921 4319 4593 4740 5151 2766 Size: 1155 398 274 147 411 3103 Fragments arranged by size: 3103 1155 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2155 2185 2588 2618 1178 Size: 30 165 30 752 30 403 30 4048 Fragments arranged by size: 4048 752 403 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5488 BseYI C'CCAG_C Cuts at: 2728 4691 5187 2728 Size: 1963 496 3029 Fragments arranged by size: 3029 1963 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2123 1163 Size: 573 387 4528 Fragments arranged by size: 4528 573 387 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2938 3699 4727 1540 Size: 275 186 937 761 1028 2301 Fragments arranged by size: 2301 1028 937 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3549 3634 4705 3549 Size: 85 1071 4332 Fragments arranged by size: 4332 1071 85 BsmI GAATG_Cn' Cuts at: 2999 3092 2999 Size: 93 5395 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2272 2807 3549 3634 4705 1391 Size: 399 482 535 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 535 482 399 85 BspEI T'CCGG_A Cuts at: 2766 5151 2766 Size: 2385 3103 BspHI T'CATG_A Cuts at: 2011 3377 5107 2011 Size: 1366 1730 2392 Fragments arranged by size: 2392 1730 1366 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2127 2843 844 Size: 1283 716 3489 Fragments arranged by size: 3489 1283 716 BsrBI CCG'CTC Cuts at: 1394 1823 3375 1394 Size: 429 1552 3507 Fragments arranged by size: 3507 1552 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3983 4157 65 Size: 1183 2735 174 1396 Fragments arranged by size: 2735 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4132 4319 1700 Size: 110 2322 187 2869 Fragments arranged by size: 2869 2322 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5488 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5488 BssSI C'ACGA_G Cuts at: 3548 4560 3548 Size: 1012 4476 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5488 BstBI TT'CG_AA Cuts at: 4292 4292 Size: 5488 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5488 BstXI CCAn_nnnn'nTGG Cuts at: 2058 2437 2058 Size: 379 5109 BstYI r'GATC_y Cuts at: 1972 2680 2818 3185 3570 3587 5039 5125 5137 Size: 708 138 367 385 17 1452 86 12 Cuts at: 5137 5483 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 708 385 367 346 138 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3997 Fragments arranged by size: 3997 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4749 Fragments arranged by size: 4749 683 56 BtsI GCAGTG_nn' Cuts at: 2669 3004 3798 3818 2669 Size: 335 794 20 4339 Fragments arranged by size: 4339 794 335 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5488 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5453 DraI TTT'AAA Cuts at: 2792 2792 Size: 5488 DraIII CAC_nnn'GTG Cuts at: 2146 2146 Size: 5488 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4495 809 Size: 507 3179 1802 Fragments arranged by size: 3179 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2108 2935 3824 Size: 54 1425 50 275 18 278 827 889 Cuts at: 3824 5453 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 827 278 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2935 1537 Size: 275 1123 4090 Fragments arranged by size: 4090 1123 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2465 3418 1353 Size: 1112 953 3423 Fragments arranged by size: 3423 1112 953 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4075 4449 4595 387 Size: 1064 138 2486 374 146 1280 Fragments arranged by size: 2486 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2412 2426 2435 2708 3551 4148 Size: 393 477 303 14 9 273 843 597 Cuts at: 4148 4935 5203 1239 Size: 787 268 1524 Fragments arranged by size: 1524 843 787 597 477 393 303 273 268 14 9 EcoNI CCTnn'n_nnAGG Cuts at: 2508 2778 2508 Size: 270 5218 EcoRI G'AATT_C Cuts at: 2797 3175 2797 Size: 378 5110 FspI TGC'GCA Cuts at: 3994 3994 Size: 5488 HaeII r_GCGC'y Cuts at: 1678 4635 1678 Size: 2957 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2588 2620 4129 4161 4203 Size: 32 135 32 1016 32 1509 32 42 Cuts at: 4203 4235 1373 Size: 32 2626 Fragments arranged by size: 2626 1509 1016 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2832 3078 669 Size: 988 281 894 246 3079 Fragments arranged by size: 3079 988 894 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5488 HpaI GTT'AAC Cuts at: 3078 3078 Size: 5488 KpnI G_GTAC'C Cuts at: 2660 2813 2660 Size: 153 5335 MfeI C'AATT_G Cuts at: 2643 3087 2643 Size: 444 5044 MluI A'CGCG_T Cuts at: 5364 5364 Size: 5488 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4602 4786 1305 Size: 3297 184 2007 Fragments arranged by size: 3297 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2110 10 Size: 54 1425 343 278 3388 Fragments arranged by size: 3388 1425 343 278 54 MslI CAynn'nnrTG Cuts at: 518 1166 2056 2237 2738 3446 3805 3964 518 Size: 648 890 181 501 708 359 159 2042 Fragments arranged by size: 2042 890 708 648 501 359 181 159 MspA1I CmG'CkG Cuts at: 1606 2537 2904 3581 4729 4974 1606 Size: 931 367 677 1148 245 2120 Fragments arranged by size: 2120 1148 931 677 367 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3997 Fragments arranged by size: 3997 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5488 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5488 NotI GC'GGCC_GC Cuts at: 2935 2935 Size: 5488 NspI r_CATG'y Cuts at: 1132 1229 2846 4391 1132 Size: 97 1617 1545 2229 Fragments arranged by size: 2229 1617 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5488 PciI A'CATG_T Cuts at: 4387 4387 Size: 5488 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5488 PmeI GTTT'AAAC Cuts at: 2792 2792 Size: 5488 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3557 3589 5070 5102 3557 Size: 32 1481 32 3943 Fragments arranged by size: 3943 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5488 PsiI TTA'TAA Cuts at: 3058 3058 Size: 5488 PstI C_TGCA'G Cuts at: 830 2840 830 Size: 2010 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5488 PvuII CAG'CTG Cuts at: 2537 2537 Size: 5488 SalI G'TCGA_C Cuts at: 1936 2830 1936 Size: 894 4594 SbfI CC_TGCA'GG Cuts at: 2840 2840 Size: 5488 ScaI AGT'ACT Cuts at: 3736 5448 3736 Size: 1712 3776 SfcI C'TryA_G Cuts at: 826 1045 2039 2836 3971 4652 4843 826 Size: 219 994 797 1135 681 191 1471 Fragments arranged by size: 1471 1135 994 797 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5488 SmaI CCC'GGG Cuts at: 1872 2815 1872 Size: 943 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3592 4493 4755 820 Size: 197 926 1649 901 262 1553 Fragments arranged by size: 1649 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3279 493 Size: 2786 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5488 SphI G_CATG'C Cuts at: 1132 2846 1132 Size: 1714 3774 SspI AAT'ATT Cuts at: 5 52 3412 5 Size: 47 3360 2081 Fragments arranged by size: 3360 2081 47 StuI AGG'CCT Cuts at: 1517 2189 2561 1517 Size: 672 372 4444 Fragments arranged by size: 4444 672 372 StyI C'CwwG_G Cuts at: 513 1065 2004 2023 2927 513 Size: 552 939 19 904 3074 Fragments arranged by size: 3074 939 904 552 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2482 2711 3515 3532 3685 3870 4315 Size: 1255 902 229 804 17 153 185 445 Cuts at: 4315 325 Size: 1498 Fragments arranged by size: 1498 1255 902 804 445 229 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2148 3734 5446 Size: 274 80 33 51 1443 171 1586 1712 Cuts at: 5446 96 Size: 138 Fragments arranged by size: 1712 1586 1443 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2155 2187 5448 5480 2155 Size: 32 3261 32 2163 Fragments arranged by size: 3261 2163 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5488 XbaI T'CTAG_A Cuts at: 2824 2824 Size: 5488 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5488 XmaI C'CCGG_G Cuts at: 1870 2813 1870 Size: 943 4545 XmnI GAAnn'nnTTC Cuts at: 3617 3617 Size: 5488 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3296 276 Size: 53 83 186 936 1762 2468 Fragments arranged by size: 2468 1762 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI