Restriction map for
pFN21AE1343
(Linear) (Six-base) MAPSORT of: KIEE1343.seq Check: 8243 from: 1 to: 783
KIEE1343.orf
With 182 enzymes: SgfI *
July 15, 2010 18:36 ..
Acc65I G'GTAC_C
Cuts at: 0 651 783
Size: 651 132
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 407 421 783
Size: 104 303 14 362
Fragments arranged by size:
362 303 104 14
AleI CACnn'nnGTG
Cuts at: 0 51 232 783
Size: 51 181 551
Fragments arranged by size:
551 181 51
ApoI r'AATT_y
Cuts at: 0 250 783
Size: 250 533
AvaI C'yCGr_G
Cuts at: 0 230 783
Size: 230 553
BanI G'GyrC_C
Cuts at: 0 234 651 783
Size: 234 417 132
Fragments arranged by size:
417 234 132
BanII G_rGCy'C
Cuts at: 0 267 783
Size: 267 516
BglII A'GATC_T
Cuts at: 0 675 783
Size: 675 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 158 270 783
Size: 158 112 513
Fragments arranged by size:
513 158 112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 703 783
Size: 430 273 80
Fragments arranged by size:
430 273 80
Bpu10I CC'TnA_GC
Cuts at: 0 266 774 783
Size: 266 508 9
Fragments arranged by size:
508 266 9
BsaWI w'CCGG_w
Cuts at: 0 761 783
Size: 761 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 150 180 583 613 783
Size: 150 30 403 30 170
Fragments arranged by size:
403 170 150 30 30
BseYI C'CCAG_C
Cuts at: 0 723 783
Size: 723 60
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 783
Size: 118 665
Bsp1286I G_dGCh'C
Cuts at: 0 267 783
Size: 267 516
BspEI T'CCGG_A
Cuts at: 0 761 783
Size: 761 22
BspHI T'CATG_A
Cuts at: 0 6 783
Size: 6 777
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 122 783
Size: 122 661
BstXI CCAn_nnnn'nTGG
Cuts at: 0 53 432 783
Size: 53 379 351
Fragments arranged by size:
379 351 53
BstYI r'GATC_y
Cuts at: 0 675 783
Size: 675 108
BtsI GCAGTG_nn'
Cuts at: 0 664 783
Size: 664 119
DraIII CAC_nnn'GTG
Cuts at: 0 141 783
Size: 141 642
EaeI y'GGCC_r
Cuts at: 0 103 783
Size: 103 680
EarI CTCTTCn'nnn_
Cuts at: 0 460 783
Size: 460 323
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 407 421 430 703 783
Size: 104 303 14 9 273 80
Fragments arranged by size:
303 273 104 80 14 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 503 773 783
Size: 503 270 10
Fragments arranged by size:
503 270 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 583 615 783
Size: 583 32 168
Fragments arranged by size:
583 168 32
KpnI G_GTAC'C
Cuts at: 0 655 783
Size: 655 128
MfeI C'AATT_G
Cuts at: 0 638 783
Size: 638 145
MscI TGG'CCA
Cuts at: 0 105 783
Size: 105 678
MslI CAynn'nnrTG
Cuts at: 0 51 232 733 783
Size: 51 181 501 50
Fragments arranged by size:
501 181 51 50
MspA1I CmG'CkG
Cuts at: 0 532 783
Size: 532 251
PvuII CAG'CTG
Cuts at: 0 532 783
Size: 532 251
SfcI C'TryA_G
Cuts at: 0 34 783
Size: 34 749
StuI AGG'CCT
Cuts at: 0 184 556 783
Size: 184 372 227
Fragments arranged by size:
372 227 184
StyI C'CwwG_G
Cuts at: 0 18 783
Size: 18 765
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 477 706 783
Size: 477 229 77
Fragments arranged by size:
477 229 77
TatI w'GTAC_w
Cuts at: 0 143 783
Size: 143 640
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 150 182 783
Size: 150 32 601
Fragments arranged by size:
601 150 32
Enzymes that do cut:
Acc65I AcuI AleI ApoI AvaI BanI BanII BglII
BmrI BpmI Bpu10I BsaWI BsaXI BseYI BsgI Bsp1286I
BspEI BspHI BspMI BstXI BstYI BtsI DraIII EaeI
EarI Eco57MI EcoNI Hin4I KpnI MfeI MscI MslI
MspA1I PvuII SfcI StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BseRI BsiEI
BsiHKAI BsiWI BsmI BsmBI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI