################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BalI BamHI BbeI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI DraI DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI Hin1I HincII HindII HindIII HinfI HpaI KasI MaeI MamI MluI MluNI MroI MscI MseI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PleI PmeI PshAI PshBI Psp1406I PvuI RcaI SalI SapI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tru9I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 869 GGTACC G^GTACC AflIII 539 ACGTGT A^C[GA][CT]GT Alw44I 952 GTGCAC G^TGCAC AlwI 1157 GGATC GGATC(4/5) Aor51HI 691 AGCGCT AGC^GCT ApaI 245 GGGCCC GGGCC^C ApaLI 952 GTGCAC G^TGCAC Asp718I 869 GGTACC G^GTACC AspI 985 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 538 CACGTG CAC^GTG BbsI 844 GTCTTC GAAGAC(2/6) BglII 1263 AGATCT A^GATCT BpuAI 844 GTCTTC GAAGAC(2/6) BsaAI 538 CACGTG [CT]AC^GT[GA] BsaWI 449 ACCGGA [AT]^CCGG[AT] BsgI 445 CTGCAC GTGCAG(16/14) BsmAI 740 GTCTC GTCTC(1/5) BsmBI 739 CGTCTC CGTCTC(1/5) BssHII 1088 GCGCGC G^CGCGC BstAPI 1004 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1263 AGATCT [GA]^GATC[CT] CpoI 901 CGGACCG CG^G[AT]CCG DraIII 568 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 406 CGGCCG C^GGCCG EclXI 406 CGGCCG C^GGCCG Eco47III 691 AGCGCT AGC^GCT Eco52I 406 CGGCCG C^GGCCG EcoNI 779 CCTTCGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FseI 636 GGCCGGCC GGCCGG^CC KpnI 869 GGTACC GGTAC^C KspI 1093 CCGCGG CCGC^GG MfeI 609 CAATTG C^AATTG MflI 1263 AGATCT [GA]^GATC[CT] MslI 753 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 609 CAATTG C^AATTG NspI 35 GCATGT [GA]CATG^[CT] PflFI 985 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 538 CACGTG CAC^GTG PmlI 538 CACGTG CAC^GTG PvuII 359 CAGCTG CAG^CTG RsrII 901 CGGACCG CG^G[AT]CCG SacI 106 GAGCTC GAGCT^C SacII 1093 CCGCGG CCGC^GG SbfI 90 CCTGCAGG CCTGCA^GG ScaI 1169 AGTACT AGT^ACT SmaI 415 CCCGGG CCC^GGG SmlI 697 CTCAAG C^T[CT][GA]AG Sse8387I 90 CCTGCAGG CCTGCA^GG Tsp509I 610 AATT ^AATT Tth111I 985 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1263 AGATCT [GA]^GATC[CT] XmaCI 415 CCCGGG C^CCGGG XmaI 415 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BglI 637 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 856 GCCATCCTGGC BseRI 73 CTCCTC GAGGAG(10/8) 76 CTCCTC BsiEI 406 CGGCCG CG[GA][CT]^CG 717 CGACCG DdeI 742 CTCAG C^T[ACGT]AG 1075 CTGAG DpnII 1158 GATC ^GATC 1264 GATC DsaI 962 CCGTGG C^C[GA][CT]GG 1093 CCGCGG EarI 42 CTCTTC CTCTTC(1/4) 79 CTCTTC EcoT14I 151 CCAAGG C^C[AT][AT]GG 593 CCAAGG HgaI 391 GACGC GACGC(5/10) 420 GACGC Ksp632I 42 CTCTTC CTCTTC(1/4) 79 CTCTTC MaeII 539 ACGT A^CGT 950 ACGT MboI 1158 GATC ^GATC 1264 GATC NdeII 1158 GATC ^GATC 1264 GATC PpuMI 95 AGGACCC [GA]G^G[AT]CC[CT] 411 GGGACCC Sau3AI 1158 GATC ^GATC 1264 GATC SfiI 636 GGCCGGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 855 GGCCATCCTGGCC StuI 69 AGGCCT AGG^CCT 485 AGGCCT StyI 151 CCAAGG C^C[AT][AT]GG 593 CCAAGG ######################################### # 3 OR MORE CUT ENZYMES ( 70 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NgoMIV NlaIII NlaIV PstI RsaI Sau96I ScrFI SfaNI SfcI TaqI TseI Tsp45I TspRI TthHB8I