(Circular) (Six-base) MAPSORT of: pFN21AE6341.seq Check: 904 from: 1 to: 5977 pFN21AE6341 5977 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2116 2116 Size: 5977 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 278 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3320 1937 Size: 1383 4594 Acc65I G'GTAC_C Cuts at: 2874 3298 2874 Size: 424 5553 AclI AA'CG_TT Cuts at: 1563 4104 4477 1563 Size: 2541 373 3063 Fragments arranged by size: 3063 2541 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4040 5424 5692 1632 Size: 2408 1384 268 1917 Fragments arranged by size: 2408 1917 1384 268 AfeI AGC'GCT Cuts at: 2698 2698 Size: 5977 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5780 AflIII A'CryG_T Cuts at: 1203 2544 4876 5853 1203 Size: 1341 2332 977 1327 Fragments arranged by size: 2332 1341 1327 977 AgeI A'CCGG_T Cuts at: 4808 4808 Size: 5977 AhdI GACnn_n'nnGTC Cuts at: 4706 4706 Size: 5977 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5977 AlwNI CAG_nnn'CTG Cuts at: 2279 2947 3015 3720 5292 2279 Size: 668 68 705 1572 2964 Fragments arranged by size: 2964 1572 705 668 68 ApaI G_GGCC'C Cuts at: 2254 2254 Size: 5977 ApaLI G'TGCA_C Cuts at: 2957 4034 5190 2957 Size: 1077 1156 3744 Fragments arranged by size: 3744 1156 1077 ApoI r'AATT_y Cuts at: 1493 3286 3512 3664 5773 1493 Size: 1793 226 152 2109 1697 Fragments arranged by size: 2109 1793 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4531 160 Size: 872 3499 1606 Fragments arranged by size: 3499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 AvaI C'yCGr_G Cuts at: 1870 1943 2232 2254 2420 2892 2930 3302 1870 Size: 73 289 22 166 472 38 372 4545 Fragments arranged by size: 4545 472 372 289 166 73 38 22 BamHI G'GATC_C Cuts at: 3307 3674 3307 Size: 367 5610 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2128 2593 2874 3298 4753 Size: 325 217 627 341 465 281 424 1455 Cuts at: 4753 618 Size: 1842 Fragments arranged by size: 1842 1455 627 465 424 341 325 281 217 BanII G_rGCy'C Cuts at: 1391 2115 2254 2784 3296 1391 Size: 724 139 530 512 4072 Fragments arranged by size: 4072 724 530 512 139 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2842 928 Size: 971 943 4063 Fragments arranged by size: 4063 971 943 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4168 4202 1903 Size: 34 2231 34 3678 Fragments arranged by size: 3678 2231 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3869 5079 3869 Size: 1210 4767 BclI T'GATC_A Cuts at: 1548 1548 Size: 5977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2648 2867 4588 Size: 107 122 71 631 605 976 219 1721 Cuts at: 4588 136 Size: 1525 Fragments arranged by size: 1721 1525 976 631 605 219 122 107 71 BglII A'GATC_T Cuts at: 3268 5972 3268 Size: 2704 3273 BlpI GC'TnA_GC Cuts at: 3394 3394 Size: 5977 Bme1580I G_kGCm'C Cuts at: 1790 2254 2598 2961 4038 5194 1790 Size: 464 344 363 1077 1156 2573 Fragments arranged by size: 2573 1156 1077 464 363 344 BmgBI CAC'GTC Cuts at: 1318 2956 1318 Size: 1638 4339 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2995 3708 4666 Size: 416 53 83 399 24 1578 713 958 Cuts at: 4666 442 Size: 1753 Fragments arranged by size: 1753 1578 958 713 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5977 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2247 2247 Size: 5977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4637 1239 Size: 3398 2579 Bpu10I CC'TnA_GC Cuts at: 3080 3080 Size: 5977 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2687 4102 4967 5265 2687 Size: 1415 865 298 3399 Fragments arranged by size: 3399 1415 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4640 882 Size: 617 3141 2219 Fragments arranged by size: 3141 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2545 3768 493 Size: 711 1341 1223 2702 Fragments arranged by size: 2702 1341 1223 711 BsaBI GATnn'nnATC Cuts at: 5971 5971 Size: 5977 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3784 4166 4801 275 Size: 53 83 186 936 2251 382 635 1451 Fragments arranged by size: 2251 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2454 4410 4808 5082 5229 5640 2454 Size: 1956 398 274 147 411 2791 Fragments arranged by size: 2791 1956 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5752 Fragments arranged by size: 5752 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2069 2072 1186 Size: 883 3 5091 Fragments arranged by size: 5091 883 3 BseYI C'CCAG_C Cuts at: 2121 2439 5180 5676 2121 Size: 318 2741 496 2422 Fragments arranged by size: 2741 2422 496 318 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2435 1163 Size: 573 699 4705 Fragments arranged by size: 4705 699 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2414 2725 3427 4188 5216 1540 Size: 275 186 413 311 702 761 1028 2301 Fragments arranged by size: 2301 1028 761 702 413 311 275 186 BsiHKAI G_wGCw'C Cuts at: 2115 2583 2631 2826 2961 4038 4123 5194 2115 Size: 468 48 195 135 1077 85 1071 2898 Fragments arranged by size: 2898 1077 1071 468 195 135 85 48 BsmI GAATG_Cn' Cuts at: 3488 3581 3488 Size: 93 5884 BsmBI CGTCTCn'nnnn_ Cuts at: 2750 2750 Size: 5977 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2254 2583 2598 2631 2784 2826 Size: 399 325 139 329 15 33 153 42 Cuts at: 2826 2961 3296 4038 4123 5194 1391 Size: 135 335 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 335 329 325 153 139 135 85 42 33 15 BspEI T'CCGG_A Cuts at: 5640 5640 Size: 5977 BspHI T'CATG_A Cuts at: 3866 5596 3866 Size: 1730 4247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2116 2277 2955 3332 844 Size: 1272 161 678 377 3489 Fragments arranged by size: 3489 1272 678 377 161 BsrBI CCG'CTC Cuts at: 1394 1823 3864 1394 Size: 429 2041 3507 Fragments arranged by size: 3507 2041 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4472 4646 65 Size: 1183 3224 174 1396 Fragments arranged by size: 3224 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2603 2642 2909 2961 3000 3129 4621 Size: 110 793 39 267 52 39 129 1492 Cuts at: 4621 4808 1700 Size: 187 2869 Fragments arranged by size: 2869 1492 793 267 187 129 110 52 39 39 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5977 BssHII G'CGCG_C Cuts at: 1925 3093 1925 Size: 1168 4809 BssSI C'ACGA_G Cuts at: 4037 5049 4037 Size: 1012 4965 BstAPI GCAn_nnn'nTGC Cuts at: 794 3015 794 Size: 2221 3756 BstBI TT'CG_AA Cuts at: 4781 4781 Size: 5977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5977 BstYI r'GATC_y Cuts at: 1972 3268 3307 3674 4059 4076 5528 5614 5626 Size: 1296 39 367 385 17 1452 86 12 Cuts at: 5626 5972 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1296 385 367 346 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2967 3098 513 Size: 552 1902 131 3392 Fragments arranged by size: 3392 1902 552 131 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5238 Fragments arranged by size: 5238 683 56 BtsI GCAGTG_nn' Cuts at: 2658 3493 4287 4307 2658 Size: 835 794 20 4328 Fragments arranged by size: 4328 835 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5942 DraI TTT'AAA Cuts at: 3281 3281 Size: 5977 DraIII CAC_nnn'GTG Cuts at: 2578 2578 Size: 5977 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4984 809 Size: 507 3668 1802 Fragments arranged by size: 3668 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2382 2411 2434 Size: 54 1425 50 275 18 552 29 23 Cuts at: 2434 2640 3424 4313 5942 8 Size: 206 784 889 1629 43 Fragments arranged by size: 1629 1425 889 784 552 275 206 54 50 43 29 23 18 EagI C'GGCC_G Cuts at: 1537 1812 2411 3424 1537 Size: 275 599 1013 4090 Fragments arranged by size: 4090 1013 599 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2053 2090 3907 1353 Size: 700 37 1817 3423 Fragments arranged by size: 3423 1817 700 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2315 2791 4564 4938 5084 387 Size: 1064 138 726 476 1773 374 146 1280 Fragments arranged by size: 1773 1280 1064 726 476 374 146 138 EcoICRI GAG'CTC Cuts at: 2113 2113 Size: 5977 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 4040 4637 5424 5692 1239 Size: 393 2408 597 787 268 1524 Fragments arranged by size: 2408 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2788 2788 Size: 5977 EcoO109I rG'GnC_Cy Cuts at: 2101 2250 2251 2417 2899 2101 Size: 149 1 166 482 5179 Fragments arranged by size: 5179 482 166 149 1 EcoRI G'AATT_C Cuts at: 3286 3664 3286 Size: 378 5599 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2785 2785 Size: 5977 FseI GG_CCGG'CC Cuts at: 2646 2646 Size: 5977 FspI TGC'GCA Cuts at: 4483 4483 Size: 5977 HaeII r_GCGC'y Cuts at: 1678 2279 2335 2700 2710 5124 1678 Size: 601 56 365 10 2414 2531 Fragments arranged by size: 2531 2414 601 365 56 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3119 3151 4618 4650 4692 Size: 32 135 32 1547 32 1467 32 42 Cuts at: 4692 4724 1373 Size: 32 2626 Fragments arranged by size: 2626 1547 1467 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3321 3567 669 Size: 988 281 1383 246 3079 Fragments arranged by size: 3079 1383 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5977 HpaI GTT'AAC Cuts at: 3567 3567 Size: 5977 KpnI G_GTAC'C Cuts at: 2878 3302 2878 Size: 424 5553 MfeI C'AATT_G Cuts at: 2614 3576 2614 Size: 962 5015 MluI A'CGCG_T Cuts at: 5853 5853 Size: 5977 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5091 5275 1305 Size: 3786 184 2007 Fragments arranged by size: 3786 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4155 Fragments arranged by size: 4155 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2762 3935 4294 4453 518 Size: 648 1596 1173 359 159 2042 Fragments arranged by size: 2042 1596 1173 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2299 2366 2439 3100 3393 4070 5218 5463 Size: 693 67 73 661 293 677 1148 245 Cuts at: 5463 1606 Size: 2120 Fragments arranged by size: 2120 1148 693 677 661 293 245 73 67 NaeI GCC'GGC Cuts at: 2605 2644 3002 2605 Size: 39 358 5580 Fragments arranged by size: 5580 358 39 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5425 NdeI CA'TA_TG Cuts at: 387 387 Size: 5977 NgoMIV G'CCGG_C Cuts at: 2603 2642 3000 2603 Size: 39 358 5580 Fragments arranged by size: 5580 358 39 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5977 NotI GC'GGCC_GC Cuts at: 3424 3424 Size: 5977 NspI r_CATG'y Cuts at: 1132 1229 2044 3335 4880 1132 Size: 97 815 1291 1545 2229 Fragments arranged by size: 2229 1545 1291 815 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5977 PciI A'CATG_T Cuts at: 4876 4876 Size: 5977 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5977 PmeI GTTT'AAAC Cuts at: 3281 3281 Size: 5977 PmlI CAC'GTG Cuts at: 2545 2545 Size: 5977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4046 4078 5559 5591 4046 Size: 32 1481 32 4432 Fragments arranged by size: 4432 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2101 2417 2101 Size: 316 5661 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5977 PsiI TTA'TAA Cuts at: 3547 3547 Size: 5977 PspOMI G'GGCC_C Cuts at: 2250 2250 Size: 5977 PstI C_TGCA'G Cuts at: 830 2074 2100 2304 3011 3329 830 Size: 1244 26 204 707 318 3478 Fragments arranged by size: 3478 1244 707 318 204 26 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5977 PvuII CAG'CTG Cuts at: 2366 2366 Size: 5977 RsrII CG'GwC_CG Cuts at: 2907 2907 Size: 5977 SacI G_AGCT'C Cuts at: 2115 2115 Size: 5977 SacII CC_GC'GG Cuts at: 3101 3101 Size: 5977 SalI G'TCGA_C Cuts at: 1936 3319 1936 Size: 1383 4594 SanDI GG'GwC_CC Cuts at: 2417 2417 Size: 5977 SbfI CC_TGCA'GG Cuts at: 2100 3329 2100 Size: 1229 4748 ScaI AGT'ACT Cuts at: 3176 4225 5937 3176 Size: 1049 1712 3216 Fragments arranged by size: 3216 1712 1049 SfcI C'TryA_G Cuts at: 826 1045 2070 2096 2300 3007 3325 4460 5141 Size: 219 1025 26 204 707 318 1135 681 Cuts at: 5141 5332 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1025 707 681 318 219 204 191 26 SfiI GGCCn_nnn'nGGCC Cuts at: 2648 2867 2648 Size: 219 5758 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5977 SmaI CCC'GGG Cuts at: 1872 2422 3304 1872 Size: 550 882 4545 Fragments arranged by size: 4545 882 550 SmlI C'TyrA_G Cuts at: 820 1017 1943 2702 4081 4982 5244 820 Size: 197 926 759 1379 901 262 1553 Fragments arranged by size: 1553 1379 926 901 759 262 197 SnaBI TAC'GTA Cuts at: 493 3768 493 Size: 3275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5977 SphI G_CATG'C Cuts at: 1132 3335 1132 Size: 2203 3774 SspI AAT'ATT Cuts at: 5 52 3901 5 Size: 47 3849 2081 Fragments arranged by size: 3849 2081 47 StuI AGG'CCT Cuts at: 1517 2076 2492 1517 Size: 559 416 5002 Fragments arranged by size: 5002 559 416 StyI C'CwwG_G Cuts at: 513 1065 2156 2598 3416 513 Size: 552 1091 442 818 3074 Fragments arranged by size: 3074 1091 818 552 442 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4004 4021 4174 4359 4804 325 Size: 1255 2424 17 153 185 445 1498 Fragments arranged by size: 2424 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3174 4223 5935 Size: 274 80 33 51 1443 1197 1049 1712 Cuts at: 5935 96 Size: 138 Fragments arranged by size: 1712 1443 1197 1049 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5937 5969 5937 Size: 32 5945 Tth111I GACn'n_nGTC Cuts at: 1316 2993 1316 Size: 1677 4300 XbaI T'CTAG_A Cuts at: 3313 3313 Size: 5977 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5977 XmaI C'CCGG_G Cuts at: 1870 2420 3302 1870 Size: 550 882 4545 Fragments arranged by size: 4545 882 550 XmnI GAAnn'nnTTC Cuts at: 4106 4106 Size: 5977 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 276 Size: 53 83 186 936 2251 2468 Fragments arranged by size: 2468 2251 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoRV FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI SapI SexAI SfoI SrfI SwaI XcmI