(Circular) (Six-base) MAPSORT of: pFN21AB9452.seq Check: 1770 from: 1 to: 6262 pFN21AB9452 6262 bp With 182 enzymes: SgfI * March 28, 2012 13:35 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4072 278 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3180 3605 1937 Size: 1243 425 4594 Fragments arranged by size: 4594 1243 425 Acc65I G'GTAC_C Cuts at: 3583 3583 Size: 6262 AclI AA'CG_TT Cuts at: 1563 4389 4762 1563 Size: 2826 373 3063 Fragments arranged by size: 3063 2826 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2224 3122 4325 5709 5977 1632 Size: 592 898 1203 1384 268 1917 Fragments arranged by size: 1917 1384 1203 898 592 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6065 AflIII A'CryG_T Cuts at: 1203 5161 6138 1203 Size: 3958 977 1327 Fragments arranged by size: 3958 1327 977 AgeI A'CCGG_T Cuts at: 5093 5093 Size: 6262 AhdI GACnn_n'nnGTC Cuts at: 3355 4991 3355 Size: 1636 4626 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6262 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2273 1184 Size: 1089 5173 AlwNI CAG_nnn'CTG Cuts at: 2494 2695 3112 4005 5577 2494 Size: 201 417 893 1572 3179 Fragments arranged by size: 3179 1572 893 417 201 ApaI G_GGCC'C Cuts at: 3119 3119 Size: 6262 ApaLI G'TGCA_C Cuts at: 4319 5475 4319 Size: 1156 5106 ApoI r'AATT_y Cuts at: 1493 2369 3292 3571 3797 3949 6058 1493 Size: 876 923 279 226 152 2109 1697 Fragments arranged by size: 2109 1697 923 876 279 226 152 AseI AT'TA_AT Cuts at: 160 1032 4816 160 Size: 872 3784 1606 Fragments arranged by size: 3784 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 AvaI C'yCGr_G Cuts at: 1870 1943 2092 2465 3587 1870 Size: 73 149 373 1122 4545 Fragments arranged by size: 4545 1122 373 149 73 BamHI G'GATC_C Cuts at: 3592 3959 3592 Size: 367 5895 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2637 2680 3318 3583 5038 Size: 325 217 627 850 43 638 265 1455 Cuts at: 5038 618 Size: 1842 Fragments arranged by size: 1842 1455 850 638 627 325 265 217 43 BanII G_rGCy'C Cuts at: 1391 2635 3119 3345 3581 1391 Size: 1244 484 226 236 4072 Fragments arranged by size: 4072 1244 484 236 226 BbeI G_GCGC'C Cuts at: 2641 2641 Size: 6262 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3074 3468 928 Size: 971 1175 394 3722 Fragments arranged by size: 3722 1175 971 394 BbvCI CC'TCA_GC Cuts at: 2057 2765 2057 Size: 708 5554 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4453 4487 1903 Size: 34 2516 34 3678 Fragments arranged by size: 3678 2516 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4154 5364 4154 Size: 1210 5052 BclI T'GATC_A Cuts at: 1548 1548 Size: 6262 BfrBI ATG'CAT Cuts at: 3094 3094 Size: 6262 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4873 136 Size: 107 122 71 631 605 337 2864 1525 Fragments arranged by size: 2864 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6257 6257 Size: 6262 BlpI GC'TnA_GC Cuts at: 3412 3679 3412 Size: 267 5995 Bme1580I G_kGCm'C Cuts at: 1790 2306 2685 3119 4323 5479 1790 Size: 516 379 434 1204 1156 2573 Fragments arranged by size: 2573 1204 1156 516 434 379 BmgBI CAC'GTC Cuts at: 1318 2715 1318 Size: 1397 4865 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2216 2997 3993 Size: 416 53 83 399 24 799 781 996 Cuts at: 3993 4951 442 Size: 958 1753 Fragments arranged by size: 1753 996 958 799 781 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6262 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2332 3153 3414 2332 Size: 821 261 5180 Fragments arranged by size: 5180 821 261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 2717 4922 1239 Size: 965 513 2205 2579 Fragments arranged by size: 2579 2205 965 513 Bpu10I CC'TnA_GC Cuts at: 2057 2624 2765 3344 2057 Size: 567 141 579 4975 Fragments arranged by size: 4975 579 567 141 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2828 3152 4387 5252 5550 2828 Size: 324 1235 865 298 3540 Fragments arranged by size: 3540 1235 865 324 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2558 4925 882 Size: 617 1059 2367 2219 Fragments arranged by size: 2367 2219 1059 617 BsaAI yAC'GTr Cuts at: 493 1204 3559 4053 493 Size: 711 2355 494 2702 Fragments arranged by size: 2702 2355 711 494 BsaBI GATnn'nnATC Cuts at: 6256 6256 Size: 6262 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 2535 2638 4069 Size: 53 83 186 936 615 387 103 1431 Cuts at: 4069 4451 5086 275 Size: 382 635 1451 Fragments arranged by size: 1451 1431 936 635 615 387 382 186 103 83 53 BsaWI w'CCGG_w Cuts at: 2745 4695 5093 5367 5514 5925 2745 Size: 1950 398 274 147 411 3082 Fragments arranged by size: 3082 1950 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6037 Fragments arranged by size: 6037 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2690 2734 2771 2774 3482 3491 3494 3497 Size: 1504 44 37 3 708 9 3 3 Cuts at: 3497 3506 3509 3512 1186 Size: 9 3 3 3936 Fragments arranged by size: 3936 1504 708 44 37 9 9 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2032 2299 2568 5465 5961 2032 Size: 267 269 2897 496 2333 Fragments arranged by size: 2897 2333 496 269 267 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2713 3283 1163 Size: 573 977 570 4142 Fragments arranged by size: 4142 977 573 570 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2022 3712 4473 5501 1540 Size: 275 186 21 1690 761 1028 2301 Fragments arranged by size: 2301 1690 1028 761 275 186 21 BsiHKAI G_wGCw'C Cuts at: 2454 3139 3345 4323 4408 5479 2454 Size: 685 206 978 85 1071 3237 Fragments arranged by size: 3237 1071 978 685 206 85 BsiWI C'GTAC_G Cuts at: 3447 3447 Size: 6262 BsmI GAATG_Cn' Cuts at: 3773 3866 3773 Size: 93 6169 BsmBI CGTCTCn'nnnn_ Cuts at: 2155 2155 Size: 6262 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2306 2454 2635 2685 3119 3139 3345 Size: 399 516 148 181 50 434 20 206 Cuts at: 3345 3581 4323 4408 5479 1391 Size: 236 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 516 434 399 236 206 181 148 85 50 20 BspEI T'CCGG_A Cuts at: 5925 5925 Size: 6262 BspHI T'CATG_A Cuts at: 3363 4151 5881 3363 Size: 788 1730 3744 Fragments arranged by size: 3744 1730 788 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3617 844 Size: 2773 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2500 3384 4149 1394 Size: 429 677 884 765 3507 Fragments arranged by size: 3507 884 765 677 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3283 4757 4931 65 Size: 1183 2035 1474 174 1396 Fragments arranged by size: 2035 1474 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3013 4906 5093 1700 Size: 110 1203 1893 187 2869 Fragments arranged by size: 2869 1893 1203 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6262 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6262 BssSI C'ACGA_G Cuts at: 4322 5334 4322 Size: 1012 5250 BstAPI GCAn_nnn'nTGC Cuts at: 794 2079 794 Size: 1285 4977 BstBI TT'CG_AA Cuts at: 5066 5066 Size: 6262 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6262 BstXI CCAn_nnnn'nTGG Cuts at: 2182 2182 Size: 6262 BstYI r'GATC_y Cuts at: 1972 2157 2340 3592 3959 4344 4361 5813 5899 Size: 185 183 1252 367 385 17 1452 86 Cuts at: 5899 5911 6257 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1252 385 367 346 185 183 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2049 2739 513 Size: 552 939 45 690 4036 Fragments arranged by size: 4036 939 690 552 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5523 Fragments arranged by size: 5523 683 56 BtsI GCAGTG_nn' Cuts at: 3529 3778 4572 4592 3529 Size: 249 794 20 5199 Fragments arranged by size: 5199 794 249 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6262 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6227 DraI TTT'AAA Cuts at: 3566 3566 Size: 6262 DraIII CAC_nnn'GTG Cuts at: 2224 2224 Size: 6262 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3014 3451 5269 809 Size: 507 1698 437 1818 1802 Fragments arranged by size: 1818 1802 1698 507 437 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 3709 4598 Size: 54 1425 50 275 18 189 1690 889 Cuts at: 4598 6227 8 Size: 1629 43 Fragments arranged by size: 1690 1629 1425 889 275 189 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2019 3709 1537 Size: 275 207 1690 4090 Fragments arranged by size: 4090 1690 275 207 EarI CTCTTCn'nnn_ Cuts at: 1353 2267 3246 3483 4192 1353 Size: 914 979 237 709 3423 Fragments arranged by size: 3423 979 914 709 237 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 3444 4849 5223 5369 387 Size: 1064 138 438 1417 1405 374 146 1280 Fragments arranged by size: 1417 1405 1280 1064 438 374 146 138 EcoICRI GAG'CTC Cuts at: 3343 3343 Size: 6262 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2224 2717 3122 4325 4922 5709 Size: 393 572 20 493 405 1203 597 787 Cuts at: 5709 5977 1239 Size: 268 1524 Fragments arranged by size: 1524 1203 787 597 572 493 405 393 268 20 EcoNI CCTnn'n_nnAGG Cuts at: 3104 3104 Size: 6262 EcoO109I rG'GnC_Cy Cuts at: 2206 2885 3109 3198 2206 Size: 679 224 89 5270 Fragments arranged by size: 5270 679 224 89 EcoRI G'AATT_C Cuts at: 3292 3571 3949 3292 Size: 279 378 5605 Fragments arranged by size: 5605 378 279 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2494 2494 Size: 6262 FspI TGC'GCA Cuts at: 4768 4768 Size: 6262 HaeII r_GCGC'y Cuts at: 1678 2641 5409 1678 Size: 963 2768 2531 Fragments arranged by size: 2768 2531 963 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3341 3373 4903 4935 4977 Size: 32 135 32 1769 32 1530 32 42 Cuts at: 4977 5009 1373 Size: 32 2626 Fragments arranged by size: 2626 1769 1530 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2662 3606 3852 669 Size: 988 281 724 944 246 3079 Fragments arranged by size: 3079 988 944 724 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6262 HpaI GTT'AAC Cuts at: 3852 3852 Size: 6262 KasI G'GCGC_C Cuts at: 2637 2637 Size: 6262 KpnI G_GTAC'C Cuts at: 3587 3587 Size: 6262 MfeI C'AATT_G Cuts at: 3861 3861 Size: 6262 MluI A'CGCG_T Cuts at: 6138 6138 Size: 6262 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3540 5376 5560 1305 Size: 2235 1836 184 2007 Fragments arranged by size: 2235 2007 1836 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4440 Fragments arranged by size: 4440 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3276 4220 4579 4738 518 Size: 648 2110 944 359 159 2042 Fragments arranged by size: 2110 2042 944 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3442 3678 4355 5503 5748 1606 Size: 1836 236 677 1148 245 2120 Fragments arranged by size: 2120 1836 1148 677 245 236 NarI GG'CG_CC Cuts at: 2638 2638 Size: 6262 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4771 Fragments arranged by size: 4771 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6262 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6262 NotI GC'GGCC_GC Cuts at: 3709 3709 Size: 6262 NsiI A_TGCA'T Cuts at: 3096 3096 Size: 6262 NspI r_CATG'y Cuts at: 1132 1229 3620 5165 1132 Size: 97 2391 1545 2229 Fragments arranged by size: 2391 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6262 PciI A'CATG_T Cuts at: 5161 5161 Size: 6262 PflMI CCAn_nnn'nTGG Cuts at: 1108 2398 1108 Size: 1290 4972 PfoI T'CCnGG_A Cuts at: 2153 3189 2153 Size: 1036 5226 PmeI GTTT'AAAC Cuts at: 3566 3566 Size: 6262 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4331 4363 5844 5876 4331 Size: 32 1481 32 4717 Fragments arranged by size: 4717 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2206 2206 Size: 6262 PshAI GACnn'nnGTC Cuts at: 1613 2784 1613 Size: 1171 5091 PsiI TTA'TAA Cuts at: 3832 3832 Size: 6262 PspOMI G'GGCC_C Cuts at: 3115 3115 Size: 6262 PstI C_TGCA'G Cuts at: 830 2707 3614 830 Size: 1877 907 3478 Fragments arranged by size: 3478 1877 907 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6262 PvuII CAG'CTG Cuts at: 3442 3442 Size: 6262 SacI G_AGCT'C Cuts at: 3345 3345 Size: 6262 SalI G'TCGA_C Cuts at: 1936 3604 1936 Size: 1668 4594 SbfI CC_TGCA'GG Cuts at: 3614 3614 Size: 6262 ScaI AGT'ACT Cuts at: 2396 4510 6222 2396 Size: 2114 1712 2436 Fragments arranged by size: 2436 2114 1712 SfcI C'TryA_G Cuts at: 826 1045 2703 3043 3610 4745 5426 5617 826 Size: 219 1658 340 567 1135 681 191 1471 Fragments arranged by size: 1658 1471 1135 681 567 340 219 191 SfoI GGC'GCC Cuts at: 2639 2639 Size: 6262 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6262 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6262 SmaI CCC'GGG Cuts at: 1872 2094 3589 1872 Size: 222 1495 4545 Fragments arranged by size: 4545 1495 222 SmlI C'TyrA_G Cuts at: 820 1017 1943 2843 3167 4366 5267 5529 820 Size: 197 926 900 324 1199 901 262 1553 Fragments arranged by size: 1553 1199 926 901 900 324 262 197 SnaBI TAC'GTA Cuts at: 493 4053 493 Size: 3560 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6262 SphI G_CATG'C Cuts at: 1132 3620 1132 Size: 2488 3774 SspI AAT'ATT Cuts at: 5 52 4186 5 Size: 47 4134 2081 Fragments arranged by size: 4134 2081 47 StuI AGG'CCT Cuts at: 1517 2477 3394 1517 Size: 960 917 4385 Fragments arranged by size: 4385 960 917 StyI C'CwwG_G Cuts at: 513 1065 2004 2202 2344 3417 3701 513 Size: 552 939 198 142 1073 284 3074 Fragments arranged by size: 3074 1073 939 552 284 198 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2235 2530 2847 3303 4289 4306 4459 Size: 1255 655 295 317 456 986 17 153 Cuts at: 4459 4644 5089 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 986 655 456 445 317 295 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2190 2394 3159 Size: 274 80 33 51 1443 213 204 765 Cuts at: 3159 4508 6220 96 Size: 1349 1712 138 Fragments arranged by size: 1712 1443 1349 765 274 213 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3118 3150 3302 3334 6222 6254 3118 Size: 32 152 32 2888 32 3126 Fragments arranged by size: 3126 2888 152 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3176 1316 Size: 1860 4402 XbaI T'CTAG_A Cuts at: 3598 3598 Size: 6262 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2179 2692 2179 Size: 513 5749 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6262 XmaI C'CCGG_G Cuts at: 1870 2092 3587 1870 Size: 222 1495 4545 Fragments arranged by size: 4545 1495 222 XmnI GAAnn'nnTTC Cuts at: 4391 4391 Size: 6262 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4070 276 Size: 53 83 186 936 2536 2468 Fragments arranged by size: 2536 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI