(Linear) (Six-base) MAPSORT of: KIBB9452.seq Check: 6210 from: 1 to: 1557 KIBB9452.orf With 182 enzymes: SgfI * March 22, 2012 15:45 .. AccI GT'mk_AC Cuts at: 0 1175 1557 Size: 1175 382 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 219 1117 1557 Size: 219 898 440 Fragments arranged by size: 898 440 219 AhdI GACnn_n'nnGTC Cuts at: 0 1350 1557 Size: 1350 207 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 268 1557 Size: 268 1289 AlwNI CAG_nnn'CTG Cuts at: 0 489 690 1107 1557 Size: 489 201 417 450 Fragments arranged by size: 489 450 417 201 ApaI G_GGCC'C Cuts at: 0 1114 1557 Size: 1114 443 ApoI r'AATT_y Cuts at: 0 364 1287 1557 Size: 364 923 270 Fragments arranged by size: 923 364 270 AvaI C'yCGr_G Cuts at: 0 87 460 1557 Size: 87 373 1097 Fragments arranged by size: 1097 373 87 BanI G'GyrC_C Cuts at: 0 632 675 1313 1557 Size: 632 43 638 244 Fragments arranged by size: 638 632 244 43 BanII G_rGCy'C Cuts at: 0 630 1114 1340 1557 Size: 630 484 226 217 Fragments arranged by size: 630 484 226 217 BbeI G_GCGC'C Cuts at: 0 636 1557 Size: 636 921 BbsI GAAGACnn'nnnn_ Cuts at: 0 1069 1463 1557 Size: 1069 394 94 Fragments arranged by size: 1069 394 94 BbvCI CC'TCA_GC Cuts at: 0 52 760 1557 Size: 52 708 797 Fragments arranged by size: 797 708 52 BfrBI ATG'CAT Cuts at: 0 1089 1557 Size: 1089 468 BlpI GC'TnA_GC Cuts at: 0 1407 1557 Size: 1407 150 Bme1580I G_kGCm'C Cuts at: 0 301 680 1114 1557 Size: 301 379 434 443 Fragments arranged by size: 443 434 379 301 BmgBI CAC'GTC Cuts at: 0 710 1557 Size: 710 847 BmrI ACTGGGnnnn_n' Cuts at: 0 211 992 1557 Size: 211 781 565 Fragments arranged by size: 781 565 211 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 327 1148 1409 1557 Size: 327 821 261 148 Fragments arranged by size: 821 327 261 148 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 712 1557 Size: 199 513 845 Fragments arranged by size: 845 513 199 Bpu10I CC'TnA_GC Cuts at: 0 52 619 760 1339 1557 Size: 52 567 141 579 218 Fragments arranged by size: 579 567 218 141 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 823 1147 1557 Size: 823 324 410 Fragments arranged by size: 823 410 324 BsaI GGTCTCn'nnnn_ Cuts at: 0 553 1557 Size: 553 1004 BsaAI yAC'GTr Cuts at: 0 1554 1557 Size: 1554 3 BsaHI Gr'CG_yC Cuts at: 0 143 530 633 1557 Size: 143 387 103 924 Fragments arranged by size: 924 387 143 103 BsaWI w'CCGG_w Cuts at: 0 740 1557 Size: 740 817 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 685 729 766 769 1477 1486 1489 1492 Size: 685 44 37 3 708 9 3 3 Cuts at: 1492 1501 1504 1507 1557 Size: 9 3 3 50 Fragments arranged by size: 708 685 50 44 37 9 9 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 27 294 563 1557 Size: 27 267 269 994 Fragments arranged by size: 994 269 267 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 708 1278 1557 Size: 708 570 279 Fragments arranged by size: 708 570 279 BsiEI CG_ry'CG Cuts at: 0 17 1557 Size: 17 1540 BsiHKAI G_wGCw'C Cuts at: 0 449 1134 1340 1557 Size: 449 685 206 217 Fragments arranged by size: 685 449 217 206 BsiWI C'GTAC_G Cuts at: 0 1442 1557 Size: 1442 115 BsmBI CGTCTCn'nnnn_ Cuts at: 0 150 1557 Size: 150 1407 Bsp1286I G_dGCh'C Cuts at: 0 301 449 630 680 1114 1134 1340 1557 Size: 301 148 181 50 434 20 206 217 Fragments arranged by size: 434 301 217 206 181 148 50 20 BspHI T'CATG_A Cuts at: 0 1358 1557 Size: 1358 199 BsrBI CCG'CTC Cuts at: 0 495 1379 1557 Size: 495 884 178 Fragments arranged by size: 884 495 178 BsrDI GCAATG_nn' Cuts at: 0 1278 1557 Size: 1278 279 BsrFI r'CCGG_y Cuts at: 0 1008 1557 Size: 1008 549 BstAPI GCAn_nnn'nTGC Cuts at: 0 74 1557 Size: 74 1483 BstXI CCAn_nnnn'nTGG Cuts at: 0 177 1557 Size: 177 1380 BstYI r'GATC_y Cuts at: 0 152 335 1557 Size: 152 183 1222 Fragments arranged by size: 1222 183 152 BtgI C'CryG_G Cuts at: 0 44 734 1557 Size: 44 690 823 Fragments arranged by size: 823 690 44 BtsI GCAGTG_nn' Cuts at: 0 1524 1557 Size: 1524 33 DraIII CAC_nnn'GTG Cuts at: 0 219 1557 Size: 219 1338 DrdI GACnn_nn'nnGTC Cuts at: 0 1009 1446 1557 Size: 1009 437 111 Fragments arranged by size: 1009 437 111 EaeI y'GGCC_r Cuts at: 0 14 1557 Size: 14 1543 EagI C'GGCC_G Cuts at: 0 14 1557 Size: 14 1543 EarI CTCTTCn'nnn_ Cuts at: 0 262 1241 1478 1557 Size: 262 979 237 79 Fragments arranged by size: 979 262 237 79 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 22 1439 1557 Size: 22 1417 118 Fragments arranged by size: 1417 118 22 EcoICRI GAG'CTC Cuts at: 0 1338 1557 Size: 1338 219 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 199 219 712 1117 1557 Size: 199 20 493 405 440 Fragments arranged by size: 493 440 405 199 20 EcoNI CCTnn'n_nnAGG Cuts at: 0 1099 1557 Size: 1099 458 EcoO109I rG'GnC_Cy Cuts at: 0 201 880 1104 1193 1557 Size: 201 679 224 89 364 Fragments arranged by size: 679 364 224 201 89 EcoRI G'AATT_C Cuts at: 0 1287 1557 Size: 1287 270 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 489 1557 Size: 489 1068 HaeII r_GCGC'y Cuts at: 0 636 1557 Size: 636 921 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1336 1368 1557 Size: 1336 32 189 Fragments arranged by size: 1336 189 32 HincII GTy'rAC Cuts at: 0 657 1557 Size: 657 900 KasI G'GCGC_C Cuts at: 0 632 1557 Size: 632 925 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1535 1557 Size: 1535 22 MslI CAynn'nnrTG Cuts at: 0 1271 1557 Size: 1271 286 MspA1I CmG'CkG Cuts at: 0 1437 1557 Size: 1437 120 NarI GG'CG_CC Cuts at: 0 633 1557 Size: 633 924 NsiI A_TGCA'T Cuts at: 0 1091 1557 Size: 1091 466 PflMI CCAn_nnn'nTGG Cuts at: 0 393 1557 Size: 393 1164 PfoI T'CCnGG_A Cuts at: 0 148 1184 1557 Size: 148 1036 373 Fragments arranged by size: 1036 373 148 PpuMI rG'GwC_Cy Cuts at: 0 201 1557 Size: 201 1356 PshAI GACnn'nnGTC Cuts at: 0 779 1557 Size: 779 778 PspOMI G'GGCC_C Cuts at: 0 1110 1557 Size: 1110 447 PstI C_TGCA'G Cuts at: 0 702 1557 Size: 702 855 PvuII CAG'CTG Cuts at: 0 1437 1557 Size: 1437 120 SacI G_AGCT'C Cuts at: 0 1340 1557 Size: 1340 217 ScaI AGT'ACT Cuts at: 0 391 1557 Size: 391 1166 SfcI C'TryA_G Cuts at: 0 698 1038 1557 Size: 698 340 519 Fragments arranged by size: 698 519 340 SfoI GGC'GCC Cuts at: 0 634 1557 Size: 634 923 SmaI CCC'GGG Cuts at: 0 89 1557 Size: 89 1468 SmlI C'TyrA_G Cuts at: 0 838 1162 1557 Size: 838 324 395 Fragments arranged by size: 838 395 324 StuI AGG'CCT Cuts at: 0 472 1389 1557 Size: 472 917 168 Fragments arranged by size: 917 472 168 StyI C'CwwG_G Cuts at: 0 197 339 1412 1557 Size: 197 142 1073 145 Fragments arranged by size: 1073 197 145 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 230 525 842 1298 1557 Size: 230 295 317 456 259 Fragments arranged by size: 456 317 295 259 230 TatI w'GTAC_w Cuts at: 0 185 389 1154 1557 Size: 185 204 765 403 Fragments arranged by size: 765 403 204 185 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1113 1145 1297 1329 1557 Size: 1113 32 152 32 228 Fragments arranged by size: 1113 228 152 32 32 Tth111I GACn'n_nGTC Cuts at: 0 1171 1557 Size: 1171 386 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 174 687 1557 Size: 174 513 870 Fragments arranged by size: 870 513 174 XmaI C'CCGG_G Cuts at: 0 87 1557 Size: 87 1470 Enzymes that do cut: AccI AcuI AhdI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI BbvCI BfrBI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BsrBI BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MmeI MslI MspA1I NarI NsiI PflMI PfoI PpuMI PshAI PspOMI PstI PvuII SacI ScaI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BclI BglI BglII BmtI BsaBI BsaXI BsmI BspEI BspMI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmnI ZraI