(Linear) (Six-base) MAPSORT of: KIEE4371.seq Check: 4183 from: 1 to: 1170 KIEE4371.orf With 182 enzymes: SgfI * March 22, 2012 17:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 302 1170 Size: 302 868 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 131 577 1170 Size: 131 446 593 Fragments arranged by size: 593 446 131 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 181 547 1170 Size: 181 366 623 Fragments arranged by size: 623 366 181 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 490 522 1170 Size: 490 32 648 Fragments arranged by size: 648 490 32 AlwNI CAG_nnn'CTG Cuts at: 0 1099 1170 Size: 1099 71 ApaI G_GGCC'C Cuts at: 0 13 198 404 1170 Size: 13 185 206 766 Fragments arranged by size: 766 206 185 13 AvaI C'yCGr_G Cuts at: 0 13 1170 Size: 13 1157 BamHI G'GATC_C Cuts at: 0 849 1170 Size: 849 321 BanI G'GyrC_C Cuts at: 0 111 261 864 1170 Size: 111 150 603 306 Fragments arranged by size: 603 306 150 111 BanII G_rGCy'C Cuts at: 0 13 184 198 404 622 808 1170 Size: 13 171 14 206 218 186 362 Fragments arranged by size: 362 218 206 186 171 14 13 BbsI GAAGACnn'nnnn_ Cuts at: 0 219 1170 Size: 219 951 BbvCI CC'TCA_GC Cuts at: 0 1161 1170 Size: 1161 9 BglI GCCn_nnn'nGGC Cuts at: 0 55 359 1170 Size: 55 304 811 Fragments arranged by size: 811 304 55 Bme1580I G_kGCm'C Cuts at: 0 13 116 198 266 404 698 1064 1170 Size: 13 103 82 68 138 294 366 106 Fragments arranged by size: 366 294 138 106 103 82 68 13 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 105 1170 Size: 105 1065 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 613 697 1018 1170 Size: 613 84 321 152 Fragments arranged by size: 613 321 152 84 Bpu10I CC'TnA_GC Cuts at: 0 634 1161 1170 Size: 634 527 9 Fragments arranged by size: 634 527 9 BsaI GGTCTCn'nnnn_ Cuts at: 0 980 1170 Size: 980 190 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 286 763 1089 1170 Size: 286 477 326 81 Fragments arranged by size: 477 326 286 81 BseYI C'CCAG_C Cuts at: 0 197 365 1010 1170 Size: 197 168 645 160 Fragments arranged by size: 645 197 168 160 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 4 1170 Size: 4 1166 BsiEI CG_ry'CG Cuts at: 0 363 1170 Size: 363 807 BsiHKAI G_wGCw'C Cuts at: 0 808 1170 Size: 808 362 Bsp1286I G_dGCh'C Cuts at: 0 13 116 184 198 266 404 622 698 Size: 13 103 68 14 68 138 218 76 Cuts at: 698 808 1064 1170 Size: 110 256 106 Fragments arranged by size: 256 218 138 110 106 103 76 68 68 14 13 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 302 680 1170 Size: 302 378 490 Fragments arranged by size: 490 378 302 BsrBI CCG'CTC Cuts at: 0 584 743 1170 Size: 584 159 427 Fragments arranged by size: 584 427 159 BsrFI r'CCGG_y Cuts at: 0 353 473 551 818 1170 Size: 353 120 78 267 352 Fragments arranged by size: 353 352 267 120 78 BstXI CCAn_nnnn'nTGG Cuts at: 0 705 1170 Size: 705 465 BstYI r'GATC_y Cuts at: 0 542 560 849 1170 Size: 542 18 289 321 Fragments arranged by size: 542 321 289 18 Bsu36I CC'TnA_GG Cuts at: 0 5 107 920 1019 1170 Size: 5 102 813 99 151 Fragments arranged by size: 813 151 102 99 5 BtgI C'CryG_G Cuts at: 0 153 396 1170 Size: 153 243 774 Fragments arranged by size: 774 243 153 BtsI GCAGTG_nn' Cuts at: 0 132 1104 1170 Size: 132 972 66 Fragments arranged by size: 972 132 66 DraIII CAC_nnn'GTG Cuts at: 0 693 1170 Size: 693 477 EaeI y'GGCC_r Cuts at: 0 26 360 825 1101 1170 Size: 26 334 465 276 69 Fragments arranged by size: 465 334 276 69 26 EagI C'GGCC_G Cuts at: 0 360 1170 Size: 360 810 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 340 1170 Size: 340 830 EcoICRI GAG'CTC Cuts at: 0 806 1170 Size: 806 364 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 131 577 613 697 1018 1170 Size: 131 446 36 84 321 152 Fragments arranged by size: 446 321 152 131 84 36 EcoNI CCTnn'n_nnAGG Cuts at: 0 1036 1170 Size: 1036 134 EcoO109I rG'GnC_Cy Cuts at: 0 9 10 194 369 401 489 597 957 Size: 9 1 184 175 32 88 108 360 Cuts at: 957 1041 1157 1170 Size: 84 116 13 Fragments arranged by size: 360 184 175 116 108 88 84 32 13 9 1 HaeII r_GCGC'y Cuts at: 0 61 1170 Size: 61 1109 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1123 1155 1170 Size: 1123 32 15 Fragments arranged by size: 1123 32 15 MscI TGG'CCA Cuts at: 0 28 1103 1170 Size: 28 1075 67 Fragments arranged by size: 1075 67 28 MspA1I CmG'CkG Cuts at: 0 155 258 365 398 912 1014 1170 Size: 155 103 107 33 514 102 156 Fragments arranged by size: 514 156 155 107 103 102 33 NspI r_CATG'y Cuts at: 0 243 795 1170 Size: 243 552 375 Fragments arranged by size: 552 375 243 PfoI T'CCnGG_A Cuts at: 0 494 590 852 964 1170 Size: 494 96 262 112 206 Fragments arranged by size: 494 262 206 112 96 PpuMI rG'GwC_Cy Cuts at: 0 369 1041 1157 1170 Size: 369 672 116 13 Fragments arranged by size: 672 369 116 13 PspOMI G'GGCC_C Cuts at: 0 9 194 400 1170 Size: 9 185 206 770 Fragments arranged by size: 770 206 185 9 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 412 444 1170 Size: 412 32 726 Fragments arranged by size: 726 412 32 PstI C_TGCA'G Cuts at: 0 55 605 662 815 1111 1170 Size: 55 550 57 153 296 59 Fragments arranged by size: 550 296 153 59 57 55 PvuII CAG'CTG Cuts at: 0 258 912 1170 Size: 258 654 258 Fragments arranged by size: 654 258 258 SacI G_AGCT'C Cuts at: 0 808 1170 Size: 808 362 SacII CC_GC'GG Cuts at: 0 156 399 1170 Size: 156 243 771 Fragments arranged by size: 771 243 156 SbfI CC_TGCA'GG Cuts at: 0 55 815 1170 Size: 55 760 355 Fragments arranged by size: 760 355 55 SfcI C'TryA_G Cuts at: 0 51 601 658 811 1107 1170 Size: 51 550 57 153 296 63 Fragments arranged by size: 550 296 153 63 57 51 SphI G_CATG'C Cuts at: 0 795 1170 Size: 795 375 StuI AGG'CCT Cuts at: 0 49 466 1170 Size: 49 417 704 Fragments arranged by size: 704 417 49 StyI C'CwwG_G Cuts at: 0 372 698 899 1142 1170 Size: 372 326 201 243 28 Fragments arranged by size: 372 326 243 201 28 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 576 882 1170 Size: 576 306 288 Fragments arranged by size: 576 306 288 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 850 1170 Size: 850 320 Enzymes that do cut: AarI AcuI AlfI AloI AlwNI ApaI AvaI BamHI BanI BanII BbsI BbvCI BglI Bme1580I BplI BpmI Bpu10I BsaI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I MscI MspA1I NspI PfoI PpuMI PspOMI PsrI PstI PvuII SacI SacII SbfI SfcI SphI StuI StyI TaqII XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DrdI EarI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI