(Circular) (Six-base) MAPSORT of: pFN21AE6343.seq Check: 2224 from: 1 to: 5638 pFN21AE6343 5638 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3448 278 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2981 1937 Size: 1044 4594 Acc65I G'GTAC_C Cuts at: 2670 2959 2670 Size: 289 5349 AclI AA'CG_TT Cuts at: 1563 3765 4138 1563 Size: 2202 373 3063 Fragments arranged by size: 3063 2202 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2404 2566 3701 5085 5353 1632 Size: 772 162 1135 1384 268 1917 Fragments arranged by size: 1917 1384 1135 772 268 162 AfeI AGC'GCT Cuts at: 2104 2104 Size: 5638 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5441 AflIII A'CryG_T Cuts at: 1203 4537 5514 1203 Size: 3334 977 1327 Fragments arranged by size: 3334 1327 977 AgeI A'CCGG_T Cuts at: 4469 4469 Size: 5638 AhdI GACnn_n'nnGTC Cuts at: 4367 4367 Size: 5638 AleI CACnn'nnGTG Cuts at: 1166 2345 1166 Size: 1179 4459 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5638 AlwNI CAG_nnn'CTG Cuts at: 2032 2589 2630 3381 4953 2032 Size: 557 41 751 1572 2717 Fragments arranged by size: 2717 1572 751 557 41 ApaLI G'TGCA_C Cuts at: 3695 4851 3695 Size: 1156 4482 ApoI r'AATT_y Cuts at: 1493 2947 3173 3325 5434 1493 Size: 1454 226 152 2109 1697 Fragments arranged by size: 2109 1697 1454 226 152 AseI AT'TA_AT Cuts at: 160 1032 4192 160 Size: 872 3160 1606 Fragments arranged by size: 3160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 AvaI C'yCGr_G Cuts at: 1870 1943 2216 2875 2963 1870 Size: 73 273 659 88 4545 Fragments arranged by size: 4545 659 273 88 73 BamHI G'GATC_C Cuts at: 2968 3335 2968 Size: 367 5271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2372 2647 2670 2959 Size: 325 217 627 279 306 275 23 289 Cuts at: 2959 4414 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 325 306 289 279 275 217 23 BanII G_rGCy'C Cuts at: 1391 2901 2957 1391 Size: 1510 56 4072 Fragments arranged by size: 4072 1510 56 BbeI G_GCGC'C Cuts at: 2070 2070 Size: 5638 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4667 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 1903 Size: 34 1892 34 3678 Fragments arranged by size: 3678 1892 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3530 4740 3530 Size: 1210 4428 BclI T'GATC_A Cuts at: 1548 1548 Size: 5638 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2123 2729 Size: 107 122 71 631 605 337 114 606 Cuts at: 2729 4249 136 Size: 1520 1525 Fragments arranged by size: 1525 1520 631 606 605 337 122 114 107 71 BglII A'GATC_T Cuts at: 2691 5633 2691 Size: 2942 2696 BlpI GC'TnA_GC Cuts at: 3055 3055 Size: 5638 Bme1580I G_kGCm'C Cuts at: 1790 3699 4855 1790 Size: 1909 1156 2573 Fragments arranged by size: 2573 1909 1156 BmgBI CAC'GTC Cuts at: 1318 2923 1318 Size: 1605 4033 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2179 2235 2646 Size: 416 53 83 399 24 762 56 411 Cuts at: 2646 3369 4327 442 Size: 723 958 1753 Fragments arranged by size: 1753 958 762 723 416 411 399 83 56 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4298 1239 Size: 3059 2579 Bpu10I CC'TnA_GC Cuts at: 2895 2895 Size: 5638 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2282 2459 3763 4628 4926 2282 Size: 177 1304 865 298 2994 Fragments arranged by size: 2994 1304 865 298 177 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4301 882 Size: 617 2802 2219 Fragments arranged by size: 2802 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2343 2347 3429 493 Size: 711 1139 4 1082 2702 Fragments arranged by size: 2702 1139 1082 711 4 BsaBI GATnn'nnATC Cuts at: 2696 5632 2696 Size: 2936 2702 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2067 2188 3445 3827 Size: 53 83 186 936 534 121 1257 382 Cuts at: 3827 4462 275 Size: 635 1451 Fragments arranged by size: 1451 1257 936 635 534 382 186 121 83 53 BsaWI w'CCGG_w Cuts at: 4071 4469 4743 4890 5301 4071 Size: 398 274 147 411 4408 Fragments arranged by size: 4408 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5413 Fragments arranged by size: 5413 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2785 1186 Size: 1599 4039 BseYI C'CCAG_C Cuts at: 2062 4841 5337 2062 Size: 2779 496 2363 Fragments arranged by size: 2779 2363 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2726 1163 Size: 573 990 4075 Fragments arranged by size: 4075 990 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3088 3849 4877 1540 Size: 275 186 1087 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3699 3784 4855 3699 Size: 85 1071 4482 Fragments arranged by size: 4482 1071 85 BsiWI C'GTAC_G Cuts at: 2343 2343 Size: 5638 BsmI GAATG_Cn' Cuts at: 3149 3242 3149 Size: 93 5545 BsmBI CGTCTCn'nnnn_ Cuts at: 2477 2477 Size: 5638 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2901 2957 3699 3784 4855 1391 Size: 399 1111 56 742 85 1071 2174 Fragments arranged by size: 2174 1111 1071 742 399 85 56 BspEI T'CCGG_A Cuts at: 5301 5301 Size: 5638 BspHI T'CATG_A Cuts at: 3527 5257 3527 Size: 1730 3908 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2993 844 Size: 2149 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2311 3525 1394 Size: 429 488 1214 3507 Fragments arranged by size: 3507 1214 488 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2193 4133 4307 65 Size: 1183 945 1940 174 1396 Fragments arranged by size: 1940 1396 1183 945 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4282 4469 1700 Size: 110 2472 187 2869 Fragments arranged by size: 2869 2472 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5638 BssHII G'CGCG_C Cuts at: 1925 2055 1925 Size: 130 5508 BssSI C'ACGA_G Cuts at: 3698 4710 3698 Size: 1012 4626 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5638 BstBI TT'CG_AA Cuts at: 4442 4442 Size: 5638 BstEII G'GTnAC_C Cuts at: 1184 2677 1184 Size: 1493 4145 BstYI r'GATC_y Cuts at: 1972 2691 2968 3335 3720 3737 5189 5275 5287 Size: 719 277 367 385 17 1452 86 12 Cuts at: 5287 5633 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 719 385 367 346 277 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4147 Fragments arranged by size: 4147 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4899 Fragments arranged by size: 4899 683 56 BtsI GCAGTG_nn' Cuts at: 2159 2536 3154 3948 3968 2159 Size: 377 618 794 20 3829 Fragments arranged by size: 3829 794 618 377 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5603 DraI TTT'AAA Cuts at: 2942 2942 Size: 5638 DraIII CAC_nnn'GTG Cuts at: 2389 2654 2389 Size: 265 5373 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4645 809 Size: 507 3329 1802 Fragments arranged by size: 3329 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3085 3974 5603 Size: 54 1425 50 275 18 1255 889 1629 Cuts at: 5603 8 Size: 43 Fragments arranged by size: 1629 1425 1255 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3085 1537 Size: 275 1273 4090 Fragments arranged by size: 4090 1273 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2423 2576 2708 2768 2789 2795 3568 1353 Size: 1070 153 132 60 21 6 773 3423 Fragments arranged by size: 3423 1070 773 153 132 60 21 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4225 4599 4745 387 Size: 1064 138 2636 374 146 1280 Fragments arranged by size: 2636 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2404 2566 3701 4298 5085 5353 1239 Size: 393 772 162 1135 597 787 268 1524 Fragments arranged by size: 1524 1135 787 772 597 393 268 162 EcoO109I rG'GnC_Cy Cuts at: 2627 2627 Size: 5638 EcoRI G'AATT_C Cuts at: 2947 3325 2947 Size: 378 5260 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2779 2779 Size: 5638 FspI TGC'GCA Cuts at: 4144 4144 Size: 5638 HaeII r_GCGC'y Cuts at: 1678 2070 2106 2761 2828 4785 1678 Size: 392 36 655 67 1957 2531 Fragments arranged by size: 2531 1957 655 392 67 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373 Size: 32 135 32 2707 32 42 32 2626 Fragments arranged by size: 2707 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2404 2982 3228 669 Size: 988 281 466 578 246 3079 Fragments arranged by size: 3079 988 578 466 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5638 HpaI GTT'AAC Cuts at: 3228 3228 Size: 5638 KasI G'GCGC_C Cuts at: 2066 2066 Size: 5638 KpnI G_GTAC'C Cuts at: 2674 2963 2674 Size: 289 5349 MfeI C'AATT_G Cuts at: 3237 3237 Size: 5638 MluI A'CGCG_T Cuts at: 5514 5514 Size: 5638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4752 4936 1305 Size: 3447 184 2007 Fragments arranged by size: 3447 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3816 Fragments arranged by size: 3816 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2198 2345 3596 3955 4114 518 Size: 648 1032 147 1251 359 159 2042 Fragments arranged by size: 2042 1251 1032 648 359 159 147 MspA1I CmG'CkG Cuts at: 1606 2062 2586 3054 3731 4879 5124 1606 Size: 456 524 468 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 524 468 456 245 NarI GG'CG_CC Cuts at: 2067 2067 Size: 5638 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4147 Fragments arranged by size: 4147 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5638 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5638 NotI GC'GGCC_GC Cuts at: 3085 3085 Size: 5638 NspI r_CATG'y Cuts at: 1132 1229 2545 2996 4541 1132 Size: 97 1316 451 1545 2229 Fragments arranged by size: 2229 1545 1316 451 97 PasI CC'CwG_GG Cuts at: 1348 2901 1348 Size: 1553 4085 PciI A'CATG_T Cuts at: 4537 4537 Size: 5638 PflMI CCAn_nnn'nTGG Cuts at: 1108 2389 1108 Size: 1281 4357 PfoI T'CCnGG_A Cuts at: 2766 2766 Size: 5638 PmeI GTTT'AAAC Cuts at: 2942 2942 Size: 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3707 3739 5220 5252 3707 Size: 32 1481 32 4093 Fragments arranged by size: 4093 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5638 PsiI TTA'TAA Cuts at: 3208 3208 Size: 5638 PstI C_TGCA'G Cuts at: 830 2082 2166 2990 830 Size: 1252 84 824 3478 Fragments arranged by size: 3478 1252 824 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5638 PvuII CAG'CTG Cuts at: 2062 2062 Size: 5638 SalI G'TCGA_C Cuts at: 1936 2980 1936 Size: 1044 4594 SapI GCTCTTCn'nnn_ Cuts at: 2423 2423 Size: 5638 SbfI CC_TGCA'GG Cuts at: 2082 2990 2082 Size: 908 4730 ScaI AGT'ACT Cuts at: 3886 5598 3886 Size: 1712 3926 SexAI A'CCwGG_T Cuts at: 2385 2673 2385 Size: 288 5350 SfcI C'TryA_G Cuts at: 826 1045 2078 2162 2986 4121 4802 4993 826 Size: 219 1033 84 824 1135 681 191 1471 Fragments arranged by size: 1471 1135 1033 824 681 219 191 84 SfoI GGC'GCC Cuts at: 2068 2068 Size: 5638 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5638 SmaI CCC'GGG Cuts at: 1872 2877 2965 1872 Size: 1005 88 4545 Fragments arranged by size: 4545 1005 88 SmlI C'TyrA_G Cuts at: 820 1017 1943 2216 2297 2474 3742 4643 4905 Size: 197 926 273 81 177 1268 901 262 Cuts at: 4905 820 Size: 1553 Fragments arranged by size: 1553 1268 926 901 273 262 197 177 81 SnaBI TAC'GTA Cuts at: 493 3429 493 Size: 2936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5638 SphI G_CATG'C Cuts at: 1132 2996 1132 Size: 1864 3774 SspI AAT'ATT Cuts at: 5 52 3562 5 Size: 47 3510 2081 Fragments arranged by size: 3510 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5638 StyI C'CwwG_G Cuts at: 513 1065 2004 3077 513 Size: 552 939 1073 3074 Fragments arranged by size: 3074 1073 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2360 2381 2665 3665 3682 3835 4020 Size: 1255 780 21 284 1000 17 153 185 Cuts at: 4020 4465 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1000 780 445 284 185 153 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2577 2661 3884 Size: 274 80 33 51 1443 600 84 1223 Cuts at: 3884 5596 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1223 600 274 138 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5598 5630 5598 Size: 32 5606 Tth111I GACn'n_nGTC Cuts at: 1316 2399 1316 Size: 1083 4555 XbaI T'CTAG_A Cuts at: 2974 2974 Size: 5638 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2200 2383 2200 Size: 183 5455 XhoI C'TCGA_G Cuts at: 1943 2216 1943 Size: 273 5365 XmaI C'CCGG_G Cuts at: 1870 2875 2963 1870 Size: 1005 88 4545 Fragments arranged by size: 4545 1005 88 XmnI GAAnn'nnTTC Cuts at: 3767 3767 Size: 5638 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3446 276 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI