(Circular) (Six-base) MAPSORT of: pFN21AE4579.seq Check: 8493 from: 1 to: 7060 pFN21AE4579 7060 bp With 182 enzymes: SgfI * March 28, 2012 14:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2069 2341 3256 2069 Size: 272 915 5873 Fragments arranged by size: 5873 915 272 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3297 4870 278 Size: 53 83 186 936 1761 1573 2468 Fragments arranged by size: 2468 1761 1573 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3864 4403 1937 Size: 1927 539 4594 Fragments arranged by size: 4594 1927 539 Acc65I G'GTAC_C Cuts at: 4381 4381 Size: 7060 AclI AA'CG_TT Cuts at: 1563 5187 5560 1563 Size: 3624 373 3063 Fragments arranged by size: 3624 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2264 3112 3164 3355 3590 3656 5123 6507 Size: 632 848 52 191 235 66 1467 1384 Cuts at: 6507 6775 1632 Size: 268 1917 Fragments arranged by size: 1917 1467 1384 848 632 268 235 191 66 52 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6863 AflIII A'CryG_T Cuts at: 1203 5959 6936 1203 Size: 4756 977 1327 Fragments arranged by size: 4756 1327 977 AgeI A'CCGG_T Cuts at: 5891 5891 Size: 7060 AhdI GACnn_n'nnGTC Cuts at: 5789 5789 Size: 7060 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7060 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2244 1184 Size: 1060 6000 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2568 2600 2568 Size: 32 7028 AlwNI CAG_nnn'CTG Cuts at: 2407 2736 4803 6375 2407 Size: 329 2067 1572 3092 Fragments arranged by size: 3092 2067 1572 329 ApaI G_GGCC'C Cuts at: 4226 4226 Size: 7060 ApaLI G'TGCA_C Cuts at: 2171 5117 6273 2171 Size: 2946 1156 2958 Fragments arranged by size: 2958 2946 1156 ApoI r'AATT_y Cuts at: 1493 3957 4369 4595 4747 6856 1493 Size: 2464 412 226 152 2109 1697 Fragments arranged by size: 2464 2109 1697 412 226 152 AseI AT'TA_AT Cuts at: 160 1032 5614 160 Size: 872 4582 1606 Fragments arranged by size: 4582 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7060 AvaI C'yCGr_G Cuts at: 1870 1943 2488 3756 4385 1870 Size: 73 545 1268 629 4545 Fragments arranged by size: 4545 1268 629 545 73 BamHI G'GATC_C Cuts at: 3697 4192 4390 4757 3697 Size: 495 198 367 6000 Fragments arranged by size: 6000 495 367 198 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2466 3674 4381 5836 618 Size: 325 217 627 679 1208 707 1455 1842 Fragments arranged by size: 1842 1455 1208 707 679 627 325 217 BanII G_rGCy'C Cuts at: 1391 2088 2489 3757 4226 4379 1391 Size: 697 401 1268 469 153 4072 Fragments arranged by size: 4072 1268 697 469 401 153 BbeI G_GCGC'C Cuts at: 3678 3678 Size: 7060 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2894 3304 3578 4024 928 Size: 971 995 410 274 446 3964 Fragments arranged by size: 3964 995 971 446 410 274 BbvCI CC'TCA_GC Cuts at: 3220 3220 Size: 7060 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5251 5285 1903 Size: 34 50 34 3230 34 3678 Fragments arranged by size: 3678 3230 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2786 4952 6162 2786 Size: 2166 1210 3684 Fragments arranged by size: 3684 2166 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7060 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2311 3654 5671 Size: 107 122 71 631 605 639 1343 2017 Cuts at: 5671 136 Size: 1525 Fragments arranged by size: 2017 1525 1343 639 631 605 122 107 71 BglII A'GATC_T Cuts at: 3435 4353 7055 3435 Size: 918 2702 3440 Fragments arranged by size: 3440 2702 918 BlpI GC'TnA_GC Cuts at: 4477 4477 Size: 7060 Bme1580I G_kGCm'C Cuts at: 1790 2150 2175 2289 2316 2724 3191 4226 5121 Size: 360 25 114 27 408 467 1035 895 Cuts at: 5121 6277 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1035 895 467 408 360 114 27 25 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7060 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2544 3181 3645 Size: 416 53 83 399 24 1127 637 464 Cuts at: 3645 4791 5749 442 Size: 1146 958 1753 Fragments arranged by size: 1753 1146 1127 958 637 464 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7060 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2234 2348 3433 3542 3575 3599 4283 4297 Size: 995 114 1085 109 33 24 684 14 Cuts at: 4297 5720 1239 Size: 1423 2579 Fragments arranged by size: 2579 1423 1085 995 684 114 109 33 24 14 Bpu10I CC'TnA_GC Cuts at: 2411 3220 2411 Size: 809 6251 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3983 5185 6050 6348 3983 Size: 1202 865 298 4695 Fragments arranged by size: 4695 1202 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5723 882 Size: 617 4224 2219 Fragments arranged by size: 4224 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3285 4851 493 Size: 711 2081 1566 2702 Fragments arranged by size: 2702 2081 1566 711 BsaBI GATnn'nnATC Cuts at: 7054 7054 Size: 7060 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3294 3675 4867 5249 Size: 53 83 186 936 1761 381 1192 382 Cuts at: 5249 5884 275 Size: 635 1451 Fragments arranged by size: 1761 1451 1192 936 635 382 381 186 83 53 BsaWI w'CCGG_w Cuts at: 5493 5891 6165 6312 6723 5493 Size: 398 274 147 411 5830 Fragments arranged by size: 5830 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2348 2378 3020 3050 3515 Size: 30 165 30 945 30 642 30 465 Cuts at: 3515 3545 1178 Size: 30 4693 Fragments arranged by size: 4693 945 642 465 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3086 3392 4361 1186 Size: 1900 306 969 3885 Fragments arranged by size: 3885 1900 969 306 BseYI C'CCAG_C Cuts at: 2163 2309 2369 2865 3741 6263 6759 2163 Size: 146 60 496 876 2522 496 2464 Fragments arranged by size: 2522 2464 876 496 496 146 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2721 3181 3363 3879 3902 1163 Size: 573 985 460 182 516 23 4321 Fragments arranged by size: 4321 985 573 516 460 182 23 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4510 5271 6299 1540 Size: 275 186 2509 761 1028 2301 Fragments arranged by size: 2509 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2175 5121 5206 6277 2175 Size: 2946 85 1071 2958 Fragments arranged by size: 2958 2946 1071 85 BsmI GAATG_Cn' Cuts at: 2263 3430 4571 4664 2263 Size: 1167 1141 93 4659 Fragments arranged by size: 4659 1167 1141 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2150 2175 2289 2316 2489 2724 Size: 399 298 62 25 114 27 173 235 Cuts at: 2724 3191 3757 4226 4379 5121 5206 6277 1391 Size: 467 566 469 153 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 566 469 467 399 298 235 173 153 114 85 62 27 25 BspEI T'CCGG_A Cuts at: 6723 6723 Size: 7060 BspHI T'CATG_A Cuts at: 4949 6679 4949 Size: 1730 5330 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2069 2341 2762 3256 3924 4266 4415 844 Size: 1225 272 421 494 668 342 149 3489 Fragments arranged by size: 3489 1225 668 494 421 342 272 149 BsrBI CCG'CTC Cuts at: 1394 1823 4947 1394 Size: 429 3124 3507 Fragments arranged by size: 3507 3124 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2468 2533 2592 3830 5555 5729 65 Size: 1183 1220 65 59 1238 1725 174 1396 Fragments arranged by size: 1725 1396 1238 1220 1183 174 65 59 BsrFI r'CCGG_y Cuts at: 1700 1810 2320 5704 5891 1700 Size: 110 510 3384 187 2869 Fragments arranged by size: 3384 2869 510 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7060 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7060 BssSI C'ACGA_G Cuts at: 3419 5120 6132 3419 Size: 1701 1012 4347 Fragments arranged by size: 4347 1701 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2026 2407 3195 794 Size: 1232 381 788 4659 Fragments arranged by size: 4659 1232 788 381 BstBI TT'CG_AA Cuts at: 5864 5864 Size: 7060 BstEII G'GTnAC_C Cuts at: 1184 3352 1184 Size: 2168 4892 BstXI CCAn_nnnn'nTGG Cuts at: 2309 2912 2960 2309 Size: 603 48 6409 Fragments arranged by size: 6409 603 48 BstYI r'GATC_y Cuts at: 1972 3435 3697 4192 4353 4390 4757 5142 5159 Size: 1463 262 495 161 37 367 385 17 Cuts at: 5159 6611 6697 6709 7055 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1463 1452 495 385 367 346 262 161 86 37 17 12 Bsu36I CC'TnA_GG Cuts at: 2613 3963 2613 Size: 1350 5710 BtgI C'CryG_G Cuts at: 513 1065 2686 2742 3614 513 Size: 552 1621 56 872 3959 Fragments arranged by size: 3959 1621 872 552 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2710 2752 3751 487 Size: 683 56 1484 42 999 3796 Fragments arranged by size: 3796 1484 999 683 56 42 BtsI GCAGTG_nn' Cuts at: 2823 3597 4576 5370 5390 2823 Size: 774 979 794 20 4493 Fragments arranged by size: 4493 979 794 774 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7060 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7025 DraI TTT'AAA Cuts at: 4364 4364 Size: 7060 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6067 809 Size: 507 4751 1802 Fragments arranged by size: 4751 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2140 4507 5396 Size: 54 1425 50 275 18 310 2367 889 Cuts at: 5396 7025 8 Size: 1629 43 Fragments arranged by size: 2367 1629 1425 889 310 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4507 1537 Size: 275 2695 4090 Fragments arranged by size: 4090 2695 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 2199 4990 1353 Size: 740 106 2791 3423 Fragments arranged by size: 3423 2791 740 106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2240 5647 6021 6167 387 Size: 1064 138 651 3407 374 146 1280 Fragments arranged by size: 3407 1280 1064 651 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2234 2264 2348 3112 3164 3355 3433 Size: 393 602 30 84 764 52 191 78 Cuts at: 3433 3542 3575 3590 3599 3656 4283 4297 5123 Size: 109 33 15 9 57 627 14 826 Cuts at: 5123 5720 6507 6775 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 826 787 764 627 602 597 393 268 191 109 84 78 57 52 33 30 15 14 9 EcoNI CCTnn'n_nnAGG Cuts at: 3182 3182 Size: 7060 EcoO109I rG'GnC_Cy Cuts at: 3175 3216 3526 3762 4222 4223 3175 Size: 41 310 236 460 1 6012 Fragments arranged by size: 6012 460 310 236 41 1 EcoRI G'AATT_C Cuts at: 4369 4747 4369 Size: 378 6682 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2941 2941 Size: 7060 FseI GG_CCGG'CC Cuts at: 2324 2324 Size: 7060 FspI TGC'GCA Cuts at: 5566 5566 Size: 7060 HaeII r_GCGC'y Cuts at: 1678 3233 3678 4302 6207 1678 Size: 1555 445 624 1905 2531 Fragments arranged by size: 2531 1905 1555 624 445 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2618 2650 2932 2964 4151 Size: 32 135 32 1046 32 282 32 1187 Cuts at: 4151 4183 5701 5733 5775 5807 1373 Size: 32 1518 32 42 32 2626 Fragments arranged by size: 2626 1518 1187 1046 282 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4404 4650 669 Size: 988 281 2466 246 3079 Fragments arranged by size: 3079 2466 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7060 HpaI GTT'AAC Cuts at: 4650 4650 Size: 7060 KasI G'GCGC_C Cuts at: 3674 3674 Size: 7060 KpnI G_GTAC'C Cuts at: 4385 4385 Size: 7060 MfeI C'AATT_G Cuts at: 4659 4659 Size: 7060 MluI A'CGCG_T Cuts at: 6936 6936 Size: 7060 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6174 6358 1305 Size: 4869 184 2007 Fragments arranged by size: 4869 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2142 10 Size: 54 1425 343 310 4928 Fragments arranged by size: 4928 1425 343 310 54 MslI CAynn'nnrTG Cuts at: 518 1166 2037 2504 2664 2972 5018 5377 5536 Size: 648 871 467 160 308 2046 359 159 Cuts at: 5536 518 Size: 2042 Fragments arranged by size: 2046 2042 871 648 467 359 308 160 159 MspA1I CmG'CkG Cuts at: 1606 2859 2865 3224 3242 3832 4476 5153 6301 Size: 1253 6 359 18 590 644 677 1148 Cuts at: 6301 6546 1606 Size: 245 2120 Fragments arranged by size: 2120 1253 1148 677 644 590 359 245 18 6 NaeI GCC'GGC Cuts at: 2322 2322 Size: 7060 NarI GG'CG_CC Cuts at: 3675 3675 Size: 7060 NcoI C'CATG_G Cuts at: 513 1065 2686 513 Size: 552 1621 4887 Fragments arranged by size: 4887 1621 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7060 NgoMIV G'CCGG_C Cuts at: 2320 2320 Size: 7060 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7060 NotI GC'GGCC_GC Cuts at: 4507 4507 Size: 7060 NspI r_CATG'y Cuts at: 1132 1229 3085 3587 4054 4418 5963 1132 Size: 97 1856 502 467 364 1545 2229 Fragments arranged by size: 2229 1856 1545 502 467 364 97 PasI CC'CwG_GG Cuts at: 1348 2047 2103 1348 Size: 699 56 6305 Fragments arranged by size: 6305 699 56 PciI A'CATG_T Cuts at: 5959 5959 Size: 7060 PflMI CCAn_nnn'nTGG Cuts at: 1108 3171 3438 1108 Size: 2063 267 4730 Fragments arranged by size: 4730 2063 267 PfoI T'CCnGG_A Cuts at: 2586 2586 Size: 7060 PmeI GTTT'AAAC Cuts at: 4364 4364 Size: 7060 PmlI CAC'GTG Cuts at: 3285 3285 Size: 7060 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5129 5161 6642 6674 5129 Size: 32 1481 32 5515 Fragments arranged by size: 5515 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3175 3216 3526 3762 3175 Size: 41 310 236 6473 Fragments arranged by size: 6473 310 236 41 PshAI GACnn'nnGTC Cuts at: 1613 3446 1613 Size: 1833 5227 PsiI TTA'TAA Cuts at: 4630 4630 Size: 7060 PspOMI G'GGCC_C Cuts at: 4222 4222 Size: 7060 PstI C_TGCA'G Cuts at: 830 2864 3229 3267 4412 830 Size: 2034 365 38 1145 3478 Fragments arranged by size: 3478 2034 1145 365 38 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7060 PvuII CAG'CTG Cuts at: 2859 2865 3224 3242 3832 2859 Size: 6 359 18 590 6087 Fragments arranged by size: 6087 590 359 18 6 SalI G'TCGA_C Cuts at: 1936 4402 1936 Size: 2466 4594 SapI GCTCTTCn'nnn_ Cuts at: 2093 2093 Size: 7060 SbfI CC_TGCA'GG Cuts at: 4412 4412 Size: 7060 ScaI AGT'ACT Cuts at: 5308 7020 5308 Size: 1712 5348 SfcI C'TryA_G Cuts at: 826 1045 2605 2854 2860 3225 3263 3766 4408 Size: 219 1560 249 6 365 38 503 642 Cuts at: 4408 5543 6224 6415 826 Size: 1135 681 191 1471 Fragments arranged by size: 1560 1471 1135 681 642 503 365 249 219 191 38 6 SfoI GGC'GCC Cuts at: 3676 3676 Size: 7060 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7060 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7060 SmaI CCC'GGG Cuts at: 1872 2490 4387 1872 Size: 618 1897 4545 Fragments arranged by size: 4545 1897 618 SmlI C'TyrA_G Cuts at: 820 1017 1943 3998 5164 6065 6327 820 Size: 197 926 2055 1166 901 262 1553 Fragments arranged by size: 2055 1553 1166 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4851 493 Size: 4358 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7060 SphI G_CATG'C Cuts at: 1132 3085 4418 1132 Size: 1953 1333 3774 Fragments arranged by size: 3774 1953 1333 SrfI GCCC'GGGC Cuts at: 2490 2490 Size: 7060 SspI AAT'ATT Cuts at: 5 52 4984 5 Size: 47 4932 2081 Fragments arranged by size: 4932 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7060 StyI C'CwwG_G Cuts at: 513 1065 2232 2686 4143 4499 513 Size: 552 1167 454 1457 356 3074 Fragments arranged by size: 3074 1457 1167 552 454 356 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2614 3627 5087 5104 5257 5442 5887 Size: 1255 1034 1013 1460 17 153 185 445 Cuts at: 5887 325 Size: 1498 Fragments arranged by size: 1498 1460 1255 1034 1013 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5306 7018 96 Size: 274 80 33 51 1443 3329 1712 138 Fragments arranged by size: 3329 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3515 3547 7020 7052 3515 Size: 32 3473 32 3523 Fragments arranged by size: 3523 3473 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7060 XbaI T'CTAG_A Cuts at: 4396 4396 Size: 7060 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3061 3061 Size: 7060 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7060 XmaI C'CCGG_G Cuts at: 1870 2488 4385 1870 Size: 618 1897 4545 Fragments arranged by size: 4545 1897 618 XmnI GAAnn'nnTTC Cuts at: 3641 5189 3641 Size: 1548 5512 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3295 4868 276 Size: 53 83 186 936 1761 1573 2468 Fragments arranged by size: 2468 1761 1573 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FspAI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SwaI