################################## # NO CUT ENZYMES ( 123 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspHI AvaII AviII AvrII BamHI BbvCI BclI BfrI BglI BglII BlnI BsaBI BseAI BsiEI BsiHKAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HindIII HinfI KpnI MamI MfeI MflI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PleI PmeI PpuMI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AcsI 911 GAATTT [GA]^AATT[CT] AgeI 805 ACCGGT A^CCGGT AhdI 286 GACGGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 911 GAATTT [GA]^AATT[CT] Asp700I 489 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 286 GACGGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1185 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1087 CTCGGG C^[CT]CG[GA]G BanII 670 GGGCTC G[GA]GC[CT]^C BbeI 1249 GGCGCC GGCGC^C BbrPI 177 CACGTG CAC^GTG BcgI 1171 CGAAGCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 816 GGATAC GTATCC(6/5) BfaI 710 CTAG C^TAG BlpI 609 GCTGAGC GC^T[ACGT]AGC BpmI 552 CTCCAG CTGGAG(16/14) Bpu1102I 609 GCTGAGC GC^T[ACGT]AGC BsaAI 177 CACGTG [CT]AC^GT[GA] BsaWI 805 ACCGGT [AT]^CCGG[AT] BseRI 1053 CTCCTC GAGGAG(10/8) BsmBI 298 GAGACG CGTCTC(1/5) BsmFI 167 GGGAC GGGAC(10/14) BsoBI 1087 CTCGGG C^[CT]CG[GA]G BspMI 31 ACCTGC ACCTGC(4/8) BstAPI 772 GCATGGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 586 CCATCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 609 GCTGAGC GC^T[ACGT]AGC DraIII 829 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1266 CCGCGG C^C[GA][CT]GG Eam1105I 286 GACGGATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 448 CTCTTC CTCTTC(1/4) HaeII 1249 GGCGCC [GA]GCGC^[CT] HincII 927 GTTAAC GT[CT]^[GA]AC HindII 927 GTTAAC GT[CT]^[GA]AC HpaI 927 GTTAAC GTT^AAC KasI 1249 GGCGCC G^GCGCC Ksp632I 448 CTCTTC CTCTTC(1/4) KspI 1266 CCGCGG CCGC^GG MaeI 710 CTAG C^TAG MslI 519 CATCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1249 GGCGCC GG^CGCC PflFI 1185 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 805 ACCGGT A^CCGGT PmaCI 177 CACGTG CAC^GTG PmlI 177 CACGTG CAC^GTG PshAI 1302 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 1266 CCGCGG CCGC^GG ScaI 629 AGTACT AGT^ACT SfoI 1249 GGCGCC GGC^GCC TaqI 317 TCGA T^CGA Tth111I 1185 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 317 TCGA T^CGA XmnI 489 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### BcnI 339 CCCGG CC^[GC]GG 1200 CCGGG BsmI 70 GAATGC GAATGC(1/-1) 59 GCATTC BsrFI 805 ACCGGT [GA]^CCGG[CT] 1175 GCCGGT BsrI 957 CCAGT ACTGG(1/-1) 1281 CCAGT Cfr10I 805 ACCGGT [GA]^CCGG[CT] 1175 GCCGGT Cfr13I 747 GGGCC G^G[ACGT]CC 1101 GGGCC DpnII 357 GATC ^GATC 738 GATC DraII 746 GGGGCCT [GA]G^G[ACGT]CC[CT] 1100 AGGGCCT EcoO109I 746 GGGGCCT [GA]G^G[ACGT]CC[CT] 1100 AGGGCCT HphI 375 GGTGA GGTGA(8/7) 63 TCACC MaeIII 211 GTCAC ^GT[ACGT]AC 284 GTGAC MboI 357 GATC ^GATC 738 GATC MseI 865 TTAA T^TAA 928 TTAA MspA1I 1003 CCGCTG C[AC]G^C[GT]G 1266 CCGCGG NciI 339 CCCGG CC^[GC]GG 1200 CCGGG NdeII 357 GATC ^GATC 738 GATC PflMI 1017 CCACTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1098 CCAGGGCCTGG Sau3AI 357 GATC ^GATC 738 GATC Sau96I 747 GGGCC G^G[ACGT]CC 1101 GGGCC Tru9I 865 TTAA T^TAA 928 TTAA Tsp45I 211 GTCAC ^GT[GC]AC 284 GTGAC Van91I 1017 CCACTACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1098 CCAGGGCCTGG XcmI 383 CCATTTATACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 518 CCATCTCAGTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI BalI BanI BbsI BbvI BmyI BpuAI BsaHI BsaI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI BstUI Cac8I CfoI DdeI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MaeII MboII MluNI MnlI MscI MspI MvaI MvnI MwoI NlaIII NlaIV PstI RsaI ScrFI SfaNI SfcI TseI Tsp509I TspRI