(Circular) (Six-base) MAPSORT of: pFN21AE9526.seq Check: 6762 from: 1 to: 6019 pFN21AE9526 6019 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3829 278 Size: 53 83 186 936 2293 2468 Fragments arranged by size: 2468 2293 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3362 1937 Size: 1425 4594 Acc65I G'GTAC_C Cuts at: 3340 3340 Size: 6019 AclI AA'CG_TT Cuts at: 1563 4146 4519 1563 Size: 2583 373 3063 Fragments arranged by size: 3063 2583 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2224 2440 3182 4082 5466 5734 1632 Size: 592 216 742 900 1384 268 1917 Fragments arranged by size: 1917 1384 900 742 592 268 216 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5822 AflIII A'CryG_T Cuts at: 1203 4918 5895 1203 Size: 3715 977 1327 Fragments arranged by size: 3715 1327 977 AgeI A'CCGG_T Cuts at: 2810 4850 2810 Size: 2040 3979 AhdI GACnn_n'nnGTC Cuts at: 2296 4748 2296 Size: 2452 3567 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6019 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6019 AlwNI CAG_nnn'CTG Cuts at: 3076 3109 3219 3762 5334 3076 Size: 33 110 543 1572 3761 Fragments arranged by size: 3761 1572 543 110 33 ApaLI G'TGCA_C Cuts at: 4076 5232 4076 Size: 1156 4863 ApoI r'AATT_y Cuts at: 1493 2916 3328 3554 3706 5815 1493 Size: 1423 412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1423 412 226 152 AseI AT'TA_AT Cuts at: 160 1032 4573 160 Size: 872 3541 1606 Fragments arranged by size: 3541 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6019 AvaI C'yCGr_G Cuts at: 1870 1943 3092 3344 1870 Size: 73 1149 252 4545 Fragments arranged by size: 4545 1149 252 73 BamHI G'GATC_C Cuts at: 3349 3716 3349 Size: 367 5652 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2221 3183 3254 3340 4795 Size: 325 217 627 434 962 71 86 1455 Cuts at: 4795 618 Size: 1842 Fragments arranged by size: 1842 1455 962 627 434 325 217 86 71 BanII G_rGCy'C Cuts at: 1391 2679 3338 1391 Size: 1288 659 4072 Fragments arranged by size: 4072 1288 659 BbeI G_GCGC'C Cuts at: 3258 3258 Size: 6019 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2230 2617 2811 928 Size: 971 331 387 194 4136 Fragments arranged by size: 4136 971 387 331 194 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3165 3199 4210 4244 1903 Size: 34 50 34 1144 34 1011 34 3678 Fragments arranged by size: 3678 1144 1011 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2815 3911 5121 2815 Size: 1096 1210 3713 Fragments arranged by size: 3713 1210 1096 BclI T'GATC_A Cuts at: 1548 1548 Size: 6019 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4630 136 Size: 107 122 71 631 605 2958 1525 Fragments arranged by size: 2958 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6014 6014 Size: 6019 BlpI GC'TnA_GC Cuts at: 2615 3436 2615 Size: 821 5198 Bme1580I G_kGCm'C Cuts at: 1790 2226 2844 3188 4080 5236 1790 Size: 436 618 344 892 1156 2573 Fragments arranged by size: 2573 1156 892 618 436 344 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6019 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3280 3750 4708 Size: 416 53 83 399 24 1863 470 958 Cuts at: 4708 442 Size: 1753 Fragments arranged by size: 1863 1753 958 470 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6019 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2542 4679 1239 Size: 1303 2137 2579 Fragments arranged by size: 2579 2137 1303 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4144 5009 5307 4144 Size: 865 298 4856 Fragments arranged by size: 4856 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2139 2204 3299 4682 882 Size: 617 640 65 1095 1383 2219 Fragments arranged by size: 2219 1383 1095 640 617 65 BsaAI yAC'GTr Cuts at: 493 1204 2184 3810 493 Size: 711 980 1626 2702 Fragments arranged by size: 2702 1626 980 711 BsaBI GATnn'nnATC Cuts at: 6013 6013 Size: 6019 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2166 3018 3255 3826 Size: 53 83 186 936 633 852 237 571 Cuts at: 3826 4208 4843 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 852 635 633 571 382 237 186 83 53 BsaWI w'CCGG_w Cuts at: 2810 4452 4850 5124 5271 5682 2810 Size: 1642 398 274 147 411 3147 Fragments arranged by size: 3147 1642 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2199 2229 3260 3290 1178 Size: 30 165 30 796 30 1031 30 3907 Fragments arranged by size: 3907 1031 796 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3049 1186 Size: 1863 4156 BseYI C'CCAG_C Cuts at: 2817 5222 5718 2817 Size: 2405 496 3118 Fragments arranged by size: 3118 2405 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2139 2450 2455 3170 1163 Size: 573 403 311 5 715 4012 Fragments arranged by size: 4012 715 573 403 311 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3469 4230 5258 1540 Size: 275 186 1468 761 1028 2301 Fragments arranged by size: 2301 1468 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4080 4165 5236 4080 Size: 85 1071 4863 Fragments arranged by size: 4863 1071 85 BsmI GAATG_Cn' Cuts at: 2064 2081 3530 3623 2064 Size: 17 1449 93 4460 Fragments arranged by size: 4460 1449 93 17 BsmBI CGTCTCn'nnnn_ Cuts at: 2297 2297 Size: 6019 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2679 2844 3188 3338 4080 4165 Size: 399 436 453 165 344 150 742 85 Cuts at: 4165 5236 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 453 436 399 344 165 150 85 BspEI T'CCGG_A Cuts at: 5682 5682 Size: 6019 BspHI T'CATG_A Cuts at: 3908 5638 3908 Size: 1730 4289 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2045 3374 844 Size: 1201 1329 3489 Fragments arranged by size: 3489 1329 1201 BsrBI CCG'CTC Cuts at: 1394 1823 3906 1394 Size: 429 2083 3507 Fragments arranged by size: 3507 2083 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4514 4688 65 Size: 1183 3266 174 1396 Fragments arranged by size: 3266 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2810 3180 4663 4850 1700 Size: 110 1000 370 1483 187 2869 Fragments arranged by size: 2869 1483 1000 370 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6019 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6019 BssSI C'ACGA_G Cuts at: 4079 5091 4079 Size: 1012 5007 BstAPI GCAn_nnn'nTGC Cuts at: 794 2783 794 Size: 1989 4030 BstBI TT'CG_AA Cuts at: 4823 4823 Size: 6019 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6019 BstXI CCAn_nnnn'nTGG Cuts at: 2598 2598 Size: 6019 BstYI r'GATC_y Cuts at: 1972 3349 3716 4101 4118 5570 5656 5668 6014 Size: 1377 367 385 17 1452 86 12 346 Cuts at: 6014 1972 Size: 1977 Fragments arranged by size: 1977 1452 1377 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3271 513 Size: 552 2206 3261 Fragments arranged by size: 3261 2206 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5280 Fragments arranged by size: 5280 683 56 BtsI GCAGTG_nn' Cuts at: 2167 2467 2575 3158 3535 4329 4349 2167 Size: 300 108 583 377 794 20 3837 Fragments arranged by size: 3837 794 583 377 300 108 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6019 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5984 DraI TTT'AAA Cuts at: 3323 3323 Size: 6019 DraIII CAC_nnn'GTG Cuts at: 2839 2839 Size: 6019 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5026 809 Size: 507 3710 1802 Fragments arranged by size: 3710 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2646 2700 2787 Size: 54 1425 50 275 18 816 54 87 Cuts at: 2787 2982 3117 3466 4355 5984 8 Size: 195 135 349 889 1629 43 Fragments arranged by size: 1629 1425 889 816 349 275 195 135 87 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3466 1537 Size: 275 1654 4090 Fragments arranged by size: 4090 1654 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2459 3949 1353 Size: 1106 1490 3423 Fragments arranged by size: 3423 1490 1106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4606 4980 5126 387 Size: 1064 138 3017 374 146 1280 Fragments arranged by size: 3017 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2224 2440 2542 3182 4082 4679 5466 Size: 393 592 216 102 640 900 597 787 Cuts at: 5466 5734 1239 Size: 268 1524 Fragments arranged by size: 1524 900 787 640 597 592 393 268 216 102 EcoO109I rG'GnC_Cy Cuts at: 2752 3106 2752 Size: 354 5665 EcoRI G'AATT_C Cuts at: 3328 3706 3328 Size: 378 5641 FspI TGC'GCA Cuts at: 4525 4525 Size: 6019 HaeII r_GCGC'y Cuts at: 1678 3258 5166 1678 Size: 1580 1908 2531 Fragments arranged by size: 2531 1908 1580 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2199 2231 2282 2314 2576 Size: 32 135 32 627 32 51 32 262 Cuts at: 2576 2608 2739 2771 4660 4692 4734 4766 1373 Size: 32 131 32 1889 32 42 32 2626 Fragments arranged by size: 2626 1889 627 262 135 131 51 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2934 3363 3609 669 Size: 988 281 996 429 246 3079 Fragments arranged by size: 3079 996 988 429 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6019 HpaI GTT'AAC Cuts at: 2934 3609 2934 Size: 675 5344 KasI G'GCGC_C Cuts at: 3254 3254 Size: 6019 KpnI G_GTAC'C Cuts at: 3344 3344 Size: 6019 MfeI C'AATT_G Cuts at: 3618 3618 Size: 6019 MluI A'CGCG_T Cuts at: 5895 5895 Size: 6019 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5133 5317 1305 Size: 3828 184 2007 Fragments arranged by size: 3828 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2648 2702 2984 10 Size: 54 1425 343 816 54 282 3045 Fragments arranged by size: 3045 1425 816 343 282 54 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 3977 4336 4495 518 Size: 648 1362 1449 359 159 2042 Fragments arranged by size: 2042 1449 1362 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3010 3273 3435 4112 5260 5505 1606 Size: 1404 263 162 677 1148 245 2120 Fragments arranged by size: 2120 1404 1148 677 263 245 162 NarI GG'CG_CC Cuts at: 3255 3255 Size: 6019 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5467 NdeI CA'TA_TG Cuts at: 387 387 Size: 6019 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6019 NotI GC'GGCC_GC Cuts at: 3466 3466 Size: 6019 NspI r_CATG'y Cuts at: 1132 1229 3377 4922 1132 Size: 97 2148 1545 2229 Fragments arranged by size: 2229 2148 1545 97 PasI CC'CwG_GG Cuts at: 1348 2398 1348 Size: 1050 4969 PciI A'CATG_T Cuts at: 4918 4918 Size: 6019 PflMI CCAn_nnn'nTGG Cuts at: 1108 3028 3109 1108 Size: 1920 81 4018 Fragments arranged by size: 4018 1920 81 PmeI GTTT'AAAC Cuts at: 3323 3323 Size: 6019 PmlI CAC'GTG Cuts at: 2184 2184 Size: 6019 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4088 4120 5601 5633 4088 Size: 32 1481 32 4474 Fragments arranged by size: 4474 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3311 1613 Size: 1698 4321 PsiI TTA'TAA Cuts at: 3589 3589 Size: 6019 PstI C_TGCA'G Cuts at: 830 2162 2634 2928 3264 3371 830 Size: 1332 472 294 336 107 3478 Fragments arranged by size: 3478 1332 472 336 294 107 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6019 SacII CC_GC'GG Cuts at: 3274 3274 Size: 6019 SalI G'TCGA_C Cuts at: 1936 3361 1936 Size: 1425 4594 SbfI CC_TGCA'GG Cuts at: 3371 3371 Size: 6019 ScaI AGT'ACT Cuts at: 2636 4267 5979 2636 Size: 1631 1712 2676 Fragments arranged by size: 2676 1712 1631 SfcI C'TryA_G Cuts at: 826 1045 2158 2335 2404 2630 2924 3260 3367 Size: 219 1113 177 69 226 294 336 107 Cuts at: 3367 4502 5183 5374 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1113 681 336 294 226 219 191 177 107 69 SfoI GGC'GCC Cuts at: 3256 3256 Size: 6019 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6019 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6019 SmaI CCC'GGG Cuts at: 1872 3346 1872 Size: 1474 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4123 5024 5286 820 Size: 197 926 2180 901 262 1553 Fragments arranged by size: 2180 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3810 493 Size: 3317 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6019 SphI G_CATG'C Cuts at: 1132 3377 1132 Size: 2245 3774 SspI AAT'ATT Cuts at: 5 52 3943 5 Size: 47 3891 2081 Fragments arranged by size: 3891 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6019 StyI C'CwwG_G Cuts at: 513 1065 3458 513 Size: 552 2393 3074 Fragments arranged by size: 3074 2393 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2085 2590 3301 4046 4063 4216 4401 Size: 1255 505 505 711 745 17 153 185 Cuts at: 4401 4846 325 Size: 445 1498 Fragments arranged by size: 1498 1255 745 711 505 505 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2634 2970 4265 Size: 274 80 33 51 1443 657 336 1295 Cuts at: 4265 5977 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1295 657 336 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3260 3292 5979 6011 3260 Size: 32 2687 32 3268 Fragments arranged by size: 3268 2687 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3193 1316 Size: 1877 4142 XbaI T'CTAG_A Cuts at: 3355 3355 Size: 6019 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2395 2530 2395 Size: 135 5884 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6019 XmaI C'CCGG_G Cuts at: 1870 3344 1870 Size: 1474 4545 XmnI GAAnn'nnTTC Cuts at: 2498 4148 2498 Size: 1650 4369 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3827 276 Size: 53 83 186 936 2293 2468 Fragments arranged by size: 2468 2293 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI