################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI Hin1I HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeII MamI MflI MluI MluNI MroI MscI MspI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI StyI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AflII 799 CTTAAG C^TTAAG AseI 33 ATTAAT AT^TAAT AsnI 33 ATTAAT AT^TAAT BanI 595 GGTGCC G^G[CT][GA]CC BbvI 24 GCAGC GCAGC(8/12) BclI 686 TGATCA T^GATCA BfrI 799 CTTAAG C^TTAAG BpmI 481 CTGGAG CTGGAG(16/14) BstF5I 256 CATCC GGATG(2/0) DraI 525 TTTAAA TTT^AAA DraII 732 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO109I 732 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 619 GAATTC G^AATTC FbaI 686 TGATCA T^GATCA FokI 256 CATCC GGATG(9/13) HaeIII 774 GGCC GG^CC HincII 885 GTTGAC GT[CT]^[GA]AC HindII 885 GTTGAC GT[CT]^[GA]AC HindIII 559 AAGCTT A^AGCTT MfeI 50 CAATTG C^AATTG MslI 905 CACTGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 505 CAGCTG C[AC]G^C[GT]G MunI 50 CAATTG C^AATTG PflMI 58 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 732 GGGTCCC [GA]G^G[AT]CC[CT] PshBI 33 ATTAAT AT^TAAT PvuII 505 CAGCTG CAG^CTG SfaNI 955 GCATC GCATC(5/9) SphI 789 GCATGC GCATG^C SspI 874 AATATT AAT^ATT TaqI 130 TCGA T^CGA TseI 24 GCAGC G^C[AT]GC Tsp45I 903 GTCAC ^GT[GC]AC TthHB8I 130 TCGA T^CGA Van91I 58 CCAGCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AccII 18 CGCG CG^CG 20 CGCG AciI 12 GCGG CCGC(-3/-1) 21 GCGG AocI 247 CCTCAGG CC^T[ACGT]AGG 776 CCTCAGG BsiYI 58 CCAGCAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 159 CCATAGCCAGG BslI 58 CCAGCAAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 159 CCATAGCCAGG BstUI 18 CGCG CG^CG 20 CGCG Bsu36I 247 CCTCAGG CC^T[ACGT]AGG 776 CCTCAGG CfoI 19 GCGC GCG^C 55 GCGC Eco81I 247 CCTCAGG CC^T[ACGT]AGG 776 CCTCAGG HhaI 19 GCGC GCG^C 55 GCGC HinP1I 19 GCGC G^CGC 55 GCGC MvnI 18 CGCG CG^CG 20 CGCG PleI 220 GACTC GAGTC(4/5) 320 GAGTC SfcI 567 CTATAG C^T[GA][CT]AG 825 CTACAG SmlI 305 CTCAAG C^T[CT][GA]AG 799 CTTAAG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AcsI AluI ApoI AvaII BsmFI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HinfI ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV NspI Sau3AI Sau96I ScrFI TfiI Tru9I Tsp509I TspRI