################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AccI AccII AccIII AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI HindIII KspI MaeI MfeI MluI MroI MspA1I MunI MvnI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AatII 109 GACGTC GACGT^C AcsI 361 GAATTT [GA]^AATT[CT] AflIII 419 ACACGT A^C[GA][CT]GT Alw44I 240 GTGCAC G^TGCAC ApaLI 240 GTGCAC G^TGCAC ApoI 361 GAATTT [GA]^AATT[CT] AvaI 667 CCCGAG C^[CT]CG[GA]G BamHI 690 GGATCC G^GATCC BanII 214 GAGCTC G[GA]GC[CT]^C BbeI 28 GGCGCC GGCGC^C BbrPI 420 CACGTG CAC^GTG BbsI 282 GAAGAC GAAGAC(2/6) BglI 632 GCCCCCTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 282 GAAGAC GAAGAC(2/6) BsaBI 36 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 749 TCCGGT [AT]^CCGG[AT] BseRI 211 GAGGAG GAGGAG(10/8) BsmAI 11 GTCTC GTCTC(1/5) BsmBI 10 CGTCTC CGTCTC(1/5) BsmFI 66 GTCCC GGGAC(10/14) BsoBI 667 CCCGAG C^[CT]CG[GA]G BstEII 618 GGTGACC G^GT[ACGT]ACC BstPI 618 GGTGACC G^GT[ACGT]ACC BstXI 407 CCAACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 690 GGATCC [GA]^GATC[CT] CfoI 29 GCGC GCG^C DsaI 431 CCATGG C^C[GA][CT]GG EcoO65I 618 GGTGACC G^GT[ACGT]ACC EcoT14I 431 CCATGG C^C[AT][AT]GG HaeII 28 GGCGCC [GA]GCGC^[CT] HapII 750 CCGG C^CGG HhaI 29 GCGC GCG^C HinP1I 29 GCGC G^CGC HincII 544 GTTAAC GT[CT]^[GA]AC HindII 544 GTTAAC GT[CT]^[GA]AC HinfI 379 GAGTC G^A[ACGT]TC HpaI 544 GTTAAC GTT^AAC HpaII 750 CCGG C^CGG KasI 28 GGCGCC G^GCGCC MamI 36 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 690 GGATCC [GA]^GATC[CT] MslI 612 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 750 CCGG C^CGG NarI 28 GGCGCC GG^CGCC NcoI 431 CCATGG C^CATGG PleI 379 GAGTC GAGTC(4/5) PmaCI 420 CACGTG CAC^GTG PmlI 420 CACGTG CAC^GTG PstI 539 CTGCAG CTGCA^G SacI 214 GAGCTC GAGCT^C ScaI 593 AGTACT AGT^ACT SfoI 28 GGCGCC GGC^GCC StyI 431 CCATGG C^C[AT][AT]GG TaqI 128 TCGA T^CGA Tsp509I 362 AATT ^AATT TthHB8I 128 TCGA T^CGA XhoII 690 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### Acc65I 55 GGTACC G^GTACC 116 GGTACC AcyI 28 GGCGCC G[GA]^CG[CT]C 109 GACGTC Asp718I 55 GGTACC G^GTACC 116 GGTACC AspHI 214 GAGCTC G[AT]GC[AT]^C 240 GTGCAC BalI 101 TGGCCA TGG^CCA 170 TGGCCA BbvI 712 GCAGC GCAGC(8/12) 845 GCAGC BsaAI 243 CACGTA [CT]AC^GT[GA] 420 CACGTG BsaHI 28 GGCGCC G[GA]^CG[CT]C 109 GACGTC BsiHKAI 214 GAGCTC G[AT]GC[AT]^C 240 GTGCAC BspMI 78 GCAGGT ACCTGC(4/8) 195 GCAGGT BsrDI 807 CATTGC GCAATG(2/0) 386 GCAATG Hin1I 28 GGCGCC G[GA]^CG[CT]C 109 GACGTC KpnI 55 GGTACC GGTAC^C 116 GGTACC MluNI 101 TGGCCA TGG^CCA 170 TGGCCA MscI 101 TGGCCA TGG^CCA 170 TGGCCA PpuMI 671 AGGACCC [GA]G^G[AT]CC[CT] 740 AGGTCCT SapI 436 GAAGAGC GCTCTTC(1/4) 216 GCTCTTC TseI 712 GCAGC G^C[AT]GC 845 GCAGC TspRI 331 CACTG CA[GC]TG[ACGT][ACGT]^ 399 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 52 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AvaII BanI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI SfcI Tru9I Tsp45I XcmI