(Circular) (Six-base) MAPSORT of: pFN21AE2384.seq Check: 2569 from: 1 to: 5653 pFN21AE2384 5653 bp With 182 enzymes: SgfI * November 11, 2011 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2074 2191 2074 Size: 117 5536 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2118 3463 278 Size: 53 83 186 936 582 1345 2468 Fragments arranged by size: 2468 1345 936 582 186 83 53 AccI GT'mk_AC Cuts at: 1937 2996 1937 Size: 1059 4594 Acc65I G'GTAC_C Cuts at: 2060 2121 2974 2060 Size: 61 853 4739 Fragments arranged by size: 4739 853 61 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2324 3716 5100 5368 1632 Size: 692 1392 1384 268 1917 Fragments arranged by size: 1917 1392 1384 692 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 2424 4552 5529 1203 Size: 1221 2128 977 1327 Fragments arranged by size: 2128 1327 1221 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5653 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AlwNI CAG_nnn'CTG Cuts at: 2661 2749 2889 3396 4968 2661 Size: 88 140 507 1572 3346 Fragments arranged by size: 3346 1572 507 140 88 ApaLI G'TGCA_C Cuts at: 2245 3710 4866 2245 Size: 1465 1156 3032 Fragments arranged by size: 3032 1465 1156 ApoI r'AATT_y Cuts at: 1493 2366 2962 3188 3340 5449 1493 Size: 873 596 226 152 2109 1697 Fragments arranged by size: 2109 1697 873 596 226 152 AseI AT'TA_AT Cuts at: 160 1032 4207 160 Size: 872 3175 1606 Fragments arranged by size: 3175 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2672 2978 1870 Size: 73 729 306 4545 Fragments arranged by size: 4545 729 306 73 BamHI G'GATC_C Cuts at: 2695 2983 3350 2695 Size: 288 367 4998 Fragments arranged by size: 4998 367 288 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2033 2060 2121 2974 4429 Size: 325 217 627 246 27 61 853 1455 Cuts at: 4429 618 Size: 1842 Fragments arranged by size: 1842 1455 853 627 325 246 217 61 27 BanII G_rGCy'C Cuts at: 1391 2223 2972 1391 Size: 832 749 4072 Fragments arranged by size: 4072 832 749 BbeI G_GCGC'C Cuts at: 2037 2037 Size: 5653 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2294 928 Size: 971 395 4287 Fragments arranged by size: 4287 971 395 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3844 3878 1903 Size: 34 1907 34 3678 Fragments arranged by size: 3678 1907 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 1548 Size: 5653 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2643 4264 136 Size: 107 122 71 631 605 971 1621 1525 Fragments arranged by size: 1621 1525 971 631 605 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 3070 3070 Size: 5653 Bme1580I G_kGCm'C Cuts at: 1790 2143 2249 3714 4870 1790 Size: 353 106 1465 1156 2573 Fragments arranged by size: 2573 1465 1156 353 106 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5653 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2397 3384 4342 Size: 416 53 83 399 24 980 987 958 Cuts at: 4342 442 Size: 1753 Fragments arranged by size: 1753 987 980 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2061 2483 2711 2911 4313 1239 Size: 822 422 228 200 1402 2579 Fragments arranged by size: 2579 1402 822 422 228 200 Bpu10I CC'TnA_GC Cuts at: 2192 2192 Size: 5653 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3778 4643 4941 3778 Size: 865 298 4490 Fragments arranged by size: 4490 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4316 882 Size: 617 2817 2219 Fragments arranged by size: 2817 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2250 2427 3444 493 Size: 711 1046 177 1017 2702 Fragments arranged by size: 2702 1046 1017 711 177 BsaBI GATnn'nnATC Cuts at: 2045 5647 2045 Size: 3602 2051 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 2115 3460 3842 Size: 53 83 186 936 501 81 1345 382 Cuts at: 3842 4477 275 Size: 635 1451 Fragments arranged by size: 1451 1345 936 635 501 382 186 83 81 53 BsaWI w'CCGG_w Cuts at: 2754 4086 4484 4758 4905 5316 2754 Size: 1332 398 274 147 411 3091 Fragments arranged by size: 3091 1332 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2884 2914 1178 Size: 30 165 30 1481 30 3917 Fragments arranged by size: 3917 1481 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 1186 Size: 1045 4608 BseYI C'CCAG_C Cuts at: 4856 5352 4856 Size: 496 5157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5080 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2223 2249 3714 3799 4870 2223 Size: 26 1465 85 1071 3006 Fragments arranged by size: 3006 1465 1071 85 26 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 BsmBI CGTCTCn'nnnn_ Cuts at: 2021 2021 Size: 5653 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2143 2223 2249 2972 3714 3799 4870 Size: 399 353 80 26 723 742 85 1071 Cuts at: 4870 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 723 399 353 85 80 26 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2074 2191 3008 844 Size: 1230 117 817 3489 Fragments arranged by size: 3489 1230 817 117 BsrBI CCG'CTC Cuts at: 1394 1823 3540 1394 Size: 429 1717 3507 Fragments arranged by size: 3507 1717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2398 2811 4148 4322 65 Size: 1183 1150 413 1337 174 1396 Fragments arranged by size: 1396 1337 1183 1150 413 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4297 4484 1700 Size: 110 2487 187 2869 Fragments arranged by size: 2869 2487 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5653 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 3713 4725 3713 Size: 1012 4641 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5653 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 2623 1184 Size: 1439 4214 BstXI CCAn_nnnn'nTGG Cuts at: 2419 2419 Size: 5653 BstYI r'GATC_y Cuts at: 1972 2695 2983 3350 3735 3752 5204 5290 5302 Size: 723 288 367 385 17 1452 86 12 Cuts at: 5302 5648 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 723 385 367 346 288 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2436 513 Size: 552 939 432 3730 Fragments arranged by size: 3730 939 552 432 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4914 Fragments arranged by size: 4914 683 56 BtsI GCAGTG_nn' Cuts at: 3169 3963 3983 3169 Size: 794 20 4839 Fragments arranged by size: 4839 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4660 809 Size: 507 3344 1802 Fragments arranged by size: 3344 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2106 2175 2339 Size: 54 1425 50 275 18 276 69 164 Cuts at: 2339 2433 3100 3989 5618 8 Size: 94 667 889 1629 43 Fragments arranged by size: 1629 1425 889 667 276 275 164 94 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2228 2436 2940 3583 1353 Size: 875 208 504 643 3423 Fragments arranged by size: 3423 875 643 504 208 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2221 2221 Size: 5653 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2324 2483 2711 2911 3716 4313 Size: 393 429 263 159 228 200 805 597 Cuts at: 4313 5100 5368 1239 Size: 787 268 1524 Fragments arranged by size: 1524 805 787 597 429 393 268 263 228 200 159 EcoO109I rG'GnC_Cy Cuts at: 2263 2658 2677 2746 2263 Size: 395 19 69 5170 Fragments arranged by size: 5170 395 69 19 EcoRI G'AATT_C Cuts at: 2962 3340 2962 Size: 378 5275 FspI TGC'GCA Cuts at: 4159 4159 Size: 5653 HaeII r_GCGC'y Cuts at: 1678 2037 4800 1678 Size: 359 2763 2531 Fragments arranged by size: 2763 2531 359 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4294 4326 4368 4400 1373 Size: 32 135 32 2722 32 42 32 2626 Fragments arranged by size: 2722 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2551 2997 3243 669 Size: 988 281 613 446 246 3079 Fragments arranged by size: 3079 988 613 446 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 2551 3243 2551 Size: 692 4961 KasI G'GCGC_C Cuts at: 2033 2033 Size: 5653 KpnI G_GTAC'C Cuts at: 2064 2125 2978 2064 Size: 61 853 4739 Fragments arranged by size: 4739 853 61 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2508 4767 4951 1305 Size: 1203 2259 184 2007 Fragments arranged by size: 2259 2007 1203 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2108 2177 10 Size: 54 1425 343 276 69 3486 Fragments arranged by size: 3486 1425 343 276 69 54 MslI CAynn'nnrTG Cuts at: 518 1166 2621 3611 3970 4129 518 Size: 648 1455 990 359 159 2042 Fragments arranged by size: 2042 1455 990 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3069 3746 4894 5139 1606 Size: 1463 677 1148 245 2120 Fragments arranged by size: 2120 1463 1148 677 245 NarI GG'CG_CC Cuts at: 2034 2034 Size: 5653 NcoI C'CATG_G Cuts at: 513 1065 2004 2436 513 Size: 552 939 432 3730 Fragments arranged by size: 3730 939 552 432 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 3011 4556 1132 Size: 97 1782 1545 2229 Fragments arranged by size: 2229 1782 1545 97 PasI CC'CwG_GG Cuts at: 1348 2661 1348 Size: 1313 4340 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5653 PfoI T'CCnGG_A Cuts at: 2688 2688 Size: 5653 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PmlI CAC'GTG Cuts at: 2427 2427 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2677 2746 2677 Size: 69 5584 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PstI C_TGCA'G Cuts at: 830 2548 3005 830 Size: 1718 457 3478 Fragments arranged by size: 3478 1718 457 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 SacI G_AGCT'C Cuts at: 2223 2223 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SapI GCTCTTCn'nnn_ Cuts at: 2228 2436 2228 Size: 208 5445 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 2600 3901 5613 2600 Size: 1301 1712 2640 Fragments arranged by size: 2640 1712 1301 SfcI C'TryA_G Cuts at: 826 1045 2544 2632 2736 3001 4136 4817 5008 Size: 219 1499 88 104 265 1135 681 191 Cuts at: 5008 826 Size: 1471 Fragments arranged by size: 1499 1471 1135 681 265 219 191 104 88 SfoI GGC'GCC Cuts at: 2035 2035 Size: 5653 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2980 1872 Size: 1108 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3757 4658 4920 820 Size: 197 926 1814 901 262 1553 Fragments arranged by size: 1814 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 3577 5 Size: 47 3525 2081 Fragments arranged by size: 3525 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2004 2436 3092 513 Size: 552 939 432 656 3074 Fragments arranged by size: 3074 939 656 552 432 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2583 3680 3697 3850 4035 4480 325 Size: 1255 1003 1097 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1097 1003 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2598 3899 5611 Size: 274 80 33 51 1443 621 1301 1712 Cuts at: 5611 96 Size: 138 Fragments arranged by size: 1712 1443 1301 621 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2884 2916 5613 5645 2884 Size: 32 2697 32 2892 Fragments arranged by size: 2892 2697 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2053 2443 2617 2053 Size: 390 174 5089 Fragments arranged by size: 5089 390 174 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2978 1870 Size: 1108 4545 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2116 3461 276 Size: 53 83 186 936 582 1345 2468 Fragments arranged by size: 2468 1345 936 582 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SrfI SwaI