(Linear) (Six-base) MAPSORT of: KIEE4431.seq Check: 5104 from: 1 to: 1350 KIEE4431.orf With 182 enzymes: SgfI * March 22, 2012 18:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 27 1350 Size: 27 1323 AatII G_ACGT'C Cuts at: 0 19 1350 Size: 19 1331 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 760 811 846 1350 Size: 367 393 51 35 504 Fragments arranged by size: 504 393 367 51 35 AfeI AGC'GCT Cuts at: 0 58 1350 Size: 58 1292 AflII C'TTAA_G Cuts at: 0 1306 1350 Size: 1306 44 AflIII A'CryG_T Cuts at: 0 1162 1350 Size: 1162 188 AhdI GACnn_n'nnGTC Cuts at: 0 349 375 1350 Size: 349 26 975 Fragments arranged by size: 975 349 26 AlwNI CAG_nnn'CTG Cuts at: 0 72 591 720 1350 Size: 72 519 129 630 Fragments arranged by size: 630 519 129 72 ApoI r'AATT_y Cuts at: 0 703 970 1350 Size: 703 267 380 Fragments arranged by size: 703 380 267 AseI AT'TA_AT Cuts at: 0 908 1350 Size: 908 442 AvaI C'yCGr_G Cuts at: 0 634 1350 Size: 634 716 BanI G'GyrC_C Cuts at: 0 319 1350 Size: 319 1031 BbsI GAAGACnn'nnnn_ Cuts at: 0 348 602 1165 1217 1350 Size: 348 254 563 52 133 Fragments arranged by size: 563 348 254 133 52 BbvCI CC'TCA_GC Cuts at: 0 334 650 1350 Size: 334 316 700 Fragments arranged by size: 700 334 316 BglII A'GATC_T Cuts at: 0 500 1350 Size: 500 850 BmgBI CAC'GTC Cuts at: 0 600 1350 Size: 600 750 BmrI ACTGGGnnnn_n' Cuts at: 0 365 875 1245 1350 Size: 365 510 370 105 Fragments arranged by size: 510 370 365 105 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 204 1350 Size: 204 1146 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 742 1036 1350 Size: 742 294 314 Fragments arranged by size: 742 314 294 Bpu10I CC'TnA_GC Cuts at: 0 334 650 1350 Size: 334 316 700 Fragments arranged by size: 700 334 316 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1270 1350 Size: 1270 80 BsaI GGTCTCn'nnnn_ Cuts at: 0 160 1350 Size: 160 1190 BsaBI GATnn'nnATC Cuts at: 0 395 1350 Size: 395 955 BsaHI Gr'CG_yC Cuts at: 0 16 1350 Size: 16 1334 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1033 1063 1350 Size: 1033 30 287 Fragments arranged by size: 1033 287 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 206 211 583 586 1279 1350 Size: 206 5 372 3 693 71 Fragments arranged by size: 693 372 206 71 5 3 BseYI C'CCAG_C Cuts at: 0 252 867 1350 Size: 252 615 483 Fragments arranged by size: 615 483 252 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1147 1350 Size: 1147 203 BsmI GAATG_Cn' Cuts at: 0 421 1350 Size: 421 929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 27 1350 Size: 27 1323 BsrDI GCAATG_nn' Cuts at: 0 1109 1350 Size: 1109 241 BssSI C'ACGA_G Cuts at: 0 84 1350 Size: 84 1266 BstXI CCAn_nnnn'nTGG Cuts at: 0 960 1350 Size: 960 390 BstYI r'GATC_y Cuts at: 0 95 500 1350 Size: 95 405 850 Fragments arranged by size: 850 405 95 BtgI C'CryG_G Cuts at: 0 323 890 1350 Size: 323 567 460 Fragments arranged by size: 567 460 323 BtsI GCAGTG_nn' Cuts at: 0 1230 1276 1350 Size: 1230 46 74 Fragments arranged by size: 1230 74 46 DraI TTT'AAA Cuts at: 0 708 1350 Size: 708 642 DraIII CAC_nnn'GTG Cuts at: 0 409 1350 Size: 409 941 EaeI y'GGCC_r Cuts at: 0 950 1350 Size: 950 400 EarI CTCTTCn'nnn_ Cuts at: 0 173 736 1136 1350 Size: 173 563 400 214 Fragments arranged by size: 563 400 214 173 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 367 742 760 811 846 1036 1350 Size: 367 375 18 51 35 190 314 Fragments arranged by size: 375 367 314 190 51 35 18 EcoO109I rG'GnC_Cy Cuts at: 0 360 1350 Size: 360 990 HaeII r_GCGC'y Cuts at: 0 60 1350 Size: 60 1290 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 217 249 335 361 367 393 651 683 Size: 217 32 86 26 6 26 258 32 Cuts at: 683 1266 1298 1350 Size: 583 32 52 Fragments arranged by size: 583 258 217 86 52 32 32 32 26 26 6 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 44 1350 Size: 44 1306 MscI TGG'CCA Cuts at: 0 952 1350 Size: 952 398 MslI CAynn'nnrTG Cuts at: 0 37 1350 Size: 37 1313 MspA1I CmG'CkG Cuts at: 0 441 720 780 1350 Size: 441 279 60 570 Fragments arranged by size: 570 441 279 60 NcoI C'CATG_G Cuts at: 0 323 1350 Size: 323 1027 PflMI CCAn_nnn'nTGG Cuts at: 0 591 948 1104 1350 Size: 591 357 156 246 Fragments arranged by size: 591 357 246 156 PfoI T'CCnGG_A Cuts at: 0 98 116 584 1115 1350 Size: 98 18 468 531 235 Fragments arranged by size: 531 468 235 98 18 PpuMI rG'GwC_Cy Cuts at: 0 360 1350 Size: 360 990 PsiI TTA'TAA Cuts at: 0 1343 1350 Size: 1343 7 PstI C_TGCA'G Cuts at: 0 64 1350 Size: 64 1286 PvuII CAG'CTG Cuts at: 0 441 720 780 1350 Size: 441 279 60 570 Fragments arranged by size: 570 441 279 60 SfcI C'TryA_G Cuts at: 0 60 1179 1350 Size: 60 1119 171 Fragments arranged by size: 1119 171 60 SmlI C'TyrA_G Cuts at: 0 634 1285 1306 1350 Size: 634 651 21 44 Fragments arranged by size: 651 634 44 21 StyI C'CwwG_G Cuts at: 0 323 1350 Size: 323 1027 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 416 1314 1350 Size: 416 898 36 Fragments arranged by size: 898 416 36 Tth111I GACn'n_nGTC Cuts at: 0 106 1350 Size: 106 1244 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 888 1350 Size: 888 462 XhoI C'TCGA_G Cuts at: 0 634 1350 Size: 634 716 XmnI GAAnn'nnTTC Cuts at: 0 1005 1350 Size: 1005 345 ZraI GAC'GTC Cuts at: 0 17 1350 Size: 17 1333 Enzymes that do cut: AarI AatII AcuI AfeI AflII AflIII AhdI AlwNI ApoI AseI AvaI BanI BbsI BbvCI BglII BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsmI BspMI BsrDI BssSI BstXI BstYI BtgI BtsI DraI DraIII EaeI EarI Eco57MI EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I NcoI PflMI PfoI PpuMI PsiI PstI PvuII SfcI SmlI StyI TaqII Tth111I XcmI XhoI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AgeI AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BanII BbeI BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmtI BsaAI BsaWI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI XbaI XmaI