################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I TspRI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AlwI 416 GGATC GGATC(4/5) AocI 502 CCTAAGG CC^T[ACGT]AGG AvaI 408 CTCGGG C^[CT]CG[GA]G BanI 423 GGCACC G^G[CT][GA]CC BfaI 79 CTAG C^TAG BsaI 295 GGTCTC GGTCTC(1/5) BseRI 61 GAGGAG GAGGAG(10/8) BsgI 345 CTGCAC GTGCAG(16/14) BsiWI 488 CGTACG C^GTACG BsoBI 408 CTCGGG C^[CT]CG[GA]G BspMI 122 ACCTGC ACCTGC(4/8) BstF5I 147 GGATG GGATG(2/0) Bsu36I 502 CCTAAGG CC^T[ACGT]AGG Cac8I 377 GCCAGC GC[ACGT]^[ACGT]GC EaeI 311 TGGCCG [CT]^GGCC[GA] Eco81I 502 CCTAAGG CC^T[ACGT]AGG EcoT14I 237 CCTTGG C^C[AT][AT]GG FokI 147 GGATG GGATG(9/13) HinfI 75 GATTC G^A[ACGT]TC MaeI 79 CTAG C^TAG MaeIII 37 GTAAC ^GT[ACGT]AC MseI 283 TTAA T^TAA MslI 99 CACTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MwoI 546 GCTGGAGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 95 CATG CATG^ PflMI 303 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 214 GACACTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 553 CTGCAG CTGCA^G ScaI 560 AGTACT AGT^ACT SfaNI 498 GATGC GCATC(5/9) SfcI 553 CTGCAG C^T[GA][CT]AG StyI 237 CCTTGG C^C[AT][AT]GG TaqI 320 TCGA T^CGA TfiI 75 GATTC G^A[AT]TC Tru9I 283 TTAA T^TAA Tsp509I 117 AATT ^AATT TthHB8I 320 TCGA T^CGA Van91I 303 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AciI 4 GCGG CCGC(-3/-1) 508 GCGG AlwNI 304 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 429 CAGAGGCTG ApaI 196 GGGCCC GGGCC^C 539 GGGCCC BsmAI 296 GTCTC GTCTC(1/5) 523 GTCTC BsmFI 368 GGGAC GGGAC(10/14) 452 GGGAC BstXI 50 CCAAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 438 CCAGGACTTTGG CfoI 340 GCGC GCG^C 387 GCGC DdeI 358 CTCAG C^T[ACGT]AG 503 CTAAG DpnII 27 GATC ^GATC 417 GATC HhaI 340 GCGC GCG^C 387 GCGC HinP1I 340 GCGC G^CGC 387 GCGC MaeII 349 ACGT A^CGT 487 ACGT MboI 27 GATC ^GATC 417 GATC NdeII 27 GATC ^GATC 417 GATC PpuMI 368 GGGACCC [GA]G^G[AT]CC[CT] 452 GGGACCC Sau3AI 27 GATC ^GATC 417 GATC ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AfaI AluI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cfr13I DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HpaII ItaI MboII MnlI MspI MvaI NciI NlaIV RsaI Sau96I ScrFI TseI